Aminotransferase and oxidoreductase nucleic acids and polypeptides and methods of using

ABSTRACT

The invention provides for aminotransferase and oxidoreductase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such aminotransferase and oxidoreductase nucleic acids and polypeptides.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a National Stage application under 35 U.S.C. §371 and claims benefit under 35 U.S.C. §119(a) of International Application No. PCT/US2008/014137 having an International Filing Date of Dec. 31, 2008, which claims the benefit of priority under 35 U.S.C. §119(e) of U.S. Application No. 61/018,814 having a filing date of Jan. 3, 2008.

CROSS REFERENCE TO RELATED APPLICATIONS

This application claims benefit of priority under 35 U.S.C. 119(e) to U.S. Application No. 61/018,814 filed Jan. 3, 2008, which is incorporated herein by reference in its entirety.

INCORPORATION BY REFERENCE

A Sequence Listing is being filed concurrently with the filing of this application under PCT AI §801(a). The accompanying Sequence Listing, identified as 07_(—)0245WO01seq.txt, is herein incorporated by reference.

Appendix 1 is being filed concurrently with the filing of this application under PCT AI §801(a). The accompanying Appendix, identified as 07_(—)0245WO01app.txt, is a table related to the Sequence Listing and is herein incorporated by reference.

TECHNICAL FIELD

This invention relates to nucleic acids and polypeptides, and more particularly to nucleic acids and polypeptides encoding aminotransferases and oxidoreductases as well as methods of using such aminotransferases and oxidoreductases.

BACKGROUND

An aminotransferase enzyme catalyzes a transamination reaction between an amino acid and an alpha-keto acid. Alpha-aminotransferases catalyze a reaction that removes the amino group from an amino acid, forming an alpha-keto acid, and transferring the amino group to a reactant α-keto acid, converting the keto acid into an amino acid. Therefore, an aminotransferase is useful in the production of amino acids.

An oxidoreductase enzyme such as a dehydrogenase catalyzes a reaction that oxidizes a substrate by transferring one or more protons and a pair of electrons to an acceptor (e.g., transfers electrons from a reductant to an oxidant). Therefore, an oxidoreductase is useful in catalyzing the oxidative deamination of amino acids to keto acids or the reductive amination of keto acids to amino acids.

SUMMARY

This disclosure provides for a number of different aminotransferase and oxidoreductase polypeptides and the nucleic acids encoding such aminotransferase and oxidoreductase polypeptides. This disclosure also provides for methods of using such aminotransferase and oxidoreductase nucleic acids and polypeptides.

In one aspect, the invention provides for methods of converting tryptophan to indole-3-pyruvate (or, alternatively, indole-3-pyruvate to tryptophan). Such methods include combining tryptophan (or indole-3-pyruvate) with a) one or more nucleic acid molecules chosen from the group consisting of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295, 297, 299, 301, 303, 305, 307, 309, 311, 313, 315, 317, 319, 321, 323, 325, 327, 329, 331, 333, 335, 337, 339, 341, 343, 345, 347, 349, 351, 353, 355, 357, 359, 361, 363, 365, 367, 369, 371, 373, 375, 377, 379, 381, 383, 385, 387, 389, 391, 393, 395, 397, 399, 401, 403, 405, 407, 409, 411, 413, 415, 417, 419, 421, 423, 425, 427, 429, 431, 433, 435, 437, 439, 441, 443, 445, 447, 449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469, 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499, 501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525, 527, 529, 531, 533, 535, 537, 539, 541, 543, 545, 547, 549, 551, 553, 555, 557, 559, 561, 563, 565, 567, 569, 571, 573, 575, 577, 579, 581, 583, 585, 587 589, 591, 593, 595, 597, 599, 601, 603, 605, 607, 609, 611, 613, 615, 617, 619, 621, 623, 625, 627, 629, 631, 633, 635, 637, 639, 641, 643, 645, 647, 649, 651, 653, 655, 657, 659, 661, 663, 665, 667, 669, 671, 673, 675, 677, 679, 681, 683, 685, 687, 689, 691, 693, 695, 697, 699, 701, 703, 705, 707, 709, 711, 713, 715, 716, 719, 721, 723, 725, 727, 729, 731, 733, 735, 737, 739, 741, 743, 745, 747, 749, 751, 753, 755, 757, 759, 761, 763, 765, 767, 769, 771, 773, 775, 777, 779, 781, 783, 785, 787, 789, 791, 793, 795, 797, 799, 801, 803, 805, 807, 809, 811, 813, 815, 817, 819, 821, 823, 825, 827, 829, 831, 833, 835, 837, 839, 841, 843, 845, 847, 849, 851, 853, 855, 857, 859, 861, 863, 865, 867, 869, 871, 873, 875, 877, 879, 881, 883, 885, 887, 889, 891, 893, 895, 897, 899, 901, 903, 905, 907, 909, 911, 913, 915, 917, 919, 921, 923, 925, 927, 929, 931, 933, 935, 937, 939, 941, 943, 945, 947, 949, 951, 953, 955, 957, 959, 961, 963, 965, 967, 969, 971, 973, and 975, wherein the one or more nucleic acid molecules encode polypeptides having aminotransferase (AT) or oxidoreductase activity; b) a variant of a), wherein the variant encodes a polypeptide having AT or oxidoreductase activity; c) a fragment of a) or b), wherein the fragment encodes a polypeptide having AT or oxidoreductase activity; d) one or more polypeptides chosen from the group consisting of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 220 G240N, 220 T241N, SEQ ID NO:220 having one or more of the mutations shown in Table 43 or Table 52, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 308, 310, 312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348, 350, 352, 354, 356, 358, 360, 362, 364, 366, 368, 370, 372, 374, 376, 378, 380, 382, 384, 386, 388, 390, 392, 394, 396, 398, 400, 402, 404, 406, 408, 410, 412, 414, 416, 418, 420, 422, 424, 426, 428, 430, 432, 434, 436, 438, 440, 442, 444, 446, 448, 450, 452, 454, 456, 458, 460, 462, 464, 466, 468, 470, 472, 474, 476, 478, 480, 482, 484, 486, 488, 490, 492, 494, 496, 498, 500, 502, 504, 506, 508, 510, 512, 514, 516, 518, 520, 522, 524, 526, 528, 530, 532, 534, 536, 538, 540, 542, 544, 546, 548, 550, 552, 554, 556, 558, 560, 562, 564, 566, 568, 570, 572, 574, 576, 578, 580, 582, 584, 586, 588, 590, 592, 594, 596, 598, 600, 602, 604, 606, 608, 610, 612, 614, 616, 618, 620, 622, 624, 626, 628, 630, 632, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, 684, 686, 688, 690, 692, 694, 696, 698, 700, 702, 704, 706, 708, 710, 712, 714, 716, 718, 720, 722, 724, 726, 728, 730, 732, 734, 736, 738, 740, 742, 744, 746, 748, 750, 752, 754, 756, 758, 760, 762, 764, 766, 768, 770, 772, 774, 776, 778, 780, 782, 784, 786, 788, 790, 792, 794, 796, 798, 800, 802, 804, 806, 808, 810, 812, 814, 816, 818, 820, 822, 824, 826, 828, 830, 832, 834, 836, 838, 840, 842, 844, 846, 848, 850, 852, 854, 856, 858, 860, 862, 864, 866, 868, 870, 870 T242N, 872, 874, 876, 878, 880, 882, 884, 886, 888, 890, 892, 894, 896, 898, 900, 902, 904, 906, 908, 910, 912, 914, 916, 918, 920, 922, 924, 926, 928, 930, 932, 934, 936, 938, 940, 942, 944, 946, 948, 950, 952, 954, 956, 958, 960, 962, 964, 966, 968, 970, 972, 974, 976, 1069, 1070, 1071, 1072 and 1073, wherein the one or more polypeptides has AT or oxidoreductase activity; e) a variant of d), wherein the variant has AT or oxidoreductase activity; or f) a fragment of d) or e), wherein the fragment has AT or oxidoreductase activity.

In another aspect, the invention provides methods of converting MP to monatin (or, alternatively, monatin to MP). Such methods generally include combining MP (or monatin) with a) one or more nucleic acid molecules chosen from the group consisting of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295, 297, 299, 301, 303, 305, 307, 309, 311, 313, 315, 317, 319, 321, 323, 325, 327, 329, 331, 333, 335, 337, 339, 341, 343, 345, 347, 349, 351, 353, 355, 357, 359, 361, 363, 365, 367, 369, 371, 373, 375, 377, 379, 381, 383, 385, 387, 389, 391, 393, 395, 397, 399, 401, 403, 405, 407, 409, 411, 413, 415, 417, 419, 421, 423, 425, 427, 429, 431, 433, 435, 437, 439, 441, 443, 445, 447, 449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469, 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499, 501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525, 527, 529, 531, 533, 535, 537, 539, 541, 543, 545, 547, 549, 551, 553, 555, 557, 559, 561, 563, 565, 567, 569, 571, 573, 575, 577, 579, 581, 583, 585, 587 589, 591, 593, 595, 597, 599, 601, 603, 605, 607, 609, 611, 613, 615, 617, 619, 621, 623, 625, 627, 629, 631, 633, 635, 637, 639, 641, 643, 645, 647, 649, 651, 653, 655, 657, 659, 661, 663, 665, 667, 669, 671, 673, 675, 677, 679, 681, 683, 685, 687, 689, 691, 693, 695, 697, 699, 701, 703, 705, 707, 709, 711, 713, 715, 716, 719, 721, 723, 725, 727, 729, 731, 733, 735, 737, 739, 741, 743, 745, 747, 749, 751, 753, 755, 757, 759, 761, 763, 765, 767, 769, 771, 773, 775, 777, 779, 781, 783, 785, 787, 789, 791, 793, 795, 797, 799, 801, 803, 805, 807, 809, 811, 813, 815, 817, 819, 821, 823, 825, 827, 829, 831, 833, 835, 837, 839, 841, 843, 845, 847, 849, 851, 853, 855, 857, 859, 861, 863, 865, 867, 869, 871, 873, 875, 877, 879, 881, 883, 885, 887, 889, 891, 893, 895, 897, 899, 901, 903, 905, 907, 909, 911, 913, 915, 917, 919, 921, 923, 925, 927, 929, 931, 933, 935, 937, 939, 941, 943, 945, 947, 949, 951, 953, 955, 957, 959, 961, 963, 965, 967, 969, 971, 973, and 975, wherein the one or more nucleic acid molecules encode polypeptides having aminotransferase (AT) or oxidoreductase activity; b) a variant of a), wherein the variant encodes a polypeptide having AT or oxidoreductase activity; c) a fragment of a) or b), wherein the fragment encodes a polypeptide having AT or oxidoreductase activity; d) one or more polypeptides chosen from the group consisting of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 220 G240N, 220 T241N, SEQ ID NO:220 having one or more of the mutations shown in Table 43 or Table 52, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 308, 310, 312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348, 350, 352, 354, 356, 358, 360, 362, 364, 366, 368, 370, 372, 374, 376, 378, 380, 382, 384, 386, 388, 390, 392, 394, 396, 398, 400, 402, 404, 406, 408, 410, 412, 414, 416, 418, 420, 422, 424, 426, 428, 430, 432, 434, 436, 438, 440, 442, 444, 446, 448, 450, 452, 454, 456, 458, 460, 462, 464, 466, 468, 470, 472, 474, 476, 478, 480, 482, 484, 486, 488, 490, 492, 494, 496, 498, 500, 502, 504, 506, 508, 510, 512, 514, 516, 518, 520, 522, 524, 526, 528, 530, 532, 534, 536, 538, 540, 542, 544, 546, 548, 550, 552, 554, 556, 558, 560, 562, 564, 566, 568, 570, 572, 574, 576, 578, 580, 582, 584, 586, 588, 590, 592, 594, 596, 598, 600, 602, 604, 606, 608, 610, 612, 614, 616, 618, 620, 622, 624, 626, 628, 630, 632, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, 684, 686, 688, 690, 692, 694, 696, 698, 700, 702, 704, 706, 708, 710, 712, 714, 716, 718, 720, 722, 724, 726, 728, 730, 732, 734, 736, 738, 740, 742, 744, 746, 748, 750, 752, 754, 756, 758, 760, 762, 764, 766, 768, 770, 772, 774, 776, 778, 780, 782, 784, 786, 788, 790, 792, 794, 796, 798, 800, 802, 804, 806, 808, 810, 812, 814, 816, 818, 820, 822, 824, 826, 828, 830, 832, 834, 836, 838, 840, 842, 844, 846, 848, 850, 852, 854, 856, 858, 860, 862, 864, 866, 868, 870, 870 T242N, 872, 874, 876, 878, 880, 882, 884, 886, 888, 890, 892, 894, 896, 898, 900, 902, 904, 906, 908, 910, 912, 914, 916, 918, 920, 922, 924, 926, 928, 930, 932, 934, 936, 938, 940, 942, 944, 946, 948, 950, 952, 954, 956, 958, 960, 962, 964, 966, 968, 970, 972, 974, 976, 1069, 1070, 1071, 1072 and 1073, wherein the one or more polypeptides has AT or oxidoreductase activity; e) a variant of d), wherein the variant has AT or oxidoreductase activity; or f) a fragment of d) or e), wherein the fragment has AT or oxidoreductase activity.

In one embodiment, the one or more nucleic acid molecules are chosen from the group consisting of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 865, 867, 869, 871, 873, 875, 877, 879, 881, 883, 885, 887, 889, 891, 893, 895, 897, 899, 901, 903, 905, 907, 909, 911, 913, 915, 917, 919, 921, 923, 925, 927, 929, 931, 933, 935, 937, 939, 941, 943, 945, 947, 949, 951, 953, 955, 957, 959, 969, 971, 973, and 975. In another embodiment, the one or more polypeptides are chosen from the group consisting of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 220 G240N, 220 T241N, SEQ ID NO:220 having one or more of the mutations shown in Table 43 or Table 52, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 866, 868, 870, 870 T242N, 872, 874, 876, 878, 880, 882, 884, 886, 888, 890, 892, 894, 896, 898, 900, 902, 904, 906, 908, 910, 912, 914, 916, 918, 920, 922, 924, 926, 928, 930, 932, 934, 936, 938, 940, 942, 944, 946, 948, 950, 952, 954, 956, 958, 960, 970, 972, 974, and 976.

In certain embodiments, the nucleic acid molecule has a sequence selected from the group consisting of SEQ ID NOs:945, 891, 893, 219, 175, 1063, 1065, and 1067. In certain embodiments, the polypeptide has a sequence selected from the group consisting of SEQ ID NOs:946, 892, 894, 220, 176, 1064, 1066, and 1068. In certain embodiments, the polypeptide has a sequence that corresponds to the consensus sequence shown in SEQ ID NO:1069 or 1070. In certain embodiments, the polypeptide has a sequence that corresponds to the consensus sequence shown in SEQ ID NO:1071, 1072, and 1073.

In some embodiments, the variant is a nucleic acid molecule that has at least 80% (e.g., at least 85%, at least 90%, at least 95%, or at least 99%) sequence identity to SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 865, 867, 869, 871, 873, 875, 877, 879, 881, 883, 885, 887, 889, 891, 893, 895, 897, 899, 901, 903, 905, 907, 909, 911, 913, 915, 917, 919, 921, 923, 925, 927, 929, 931, 933, 935, 937, 939, 941, 943, 945, 947, 949, 951, 953, 955, 957, 959, 969, 971, 973, and 975.

In some embodiments, the variant is a polypeptide that has at least 80% (e.g., at least 85%, at least 90%, at least 95%, or at least 99%) sequence identity to SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 220 G240N, 220 T241N, SEQ ID NO:220 having one or more of the mutations shown in Table 43 or Table 52, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 866, 868, 870, 870 T242N, 872, 874, 876, 878, 880, 882, 884, 886, 888, 890, 892, 894, 896, 898, 900, 902, 904, 906, 908, 910, 912, 914, 916, 918, 920, 922, 924, 926, 928, 930, 932, 934, 936, 938, 940, 942, 944, 946, 948, 950, 952, 954, 956, 958, 960, 970, 972, 974, and 976.

In one embodiment, the variant is a polypeptide that has at least 65% (e.g., at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or at least 99%) sequence identity to SEQ ID NO:220. In another embodiment, the variant is a polypeptide that has at least 80% (e.g., at least 85%, at least 90%, at least 95%, or at least 99%) sequence identity to SEQ ID NO:870. In yet another embodiment, the variant is a polypeptide that has at least 65% (e.g., at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or at least 99%) sequence identity to SEQ ID NO:894.

In certain embodiments, the variant is a mutant. Representative mutants include, without limitation, a mutant having a mutation at the residue that aligns with residue 243 of DAT 4978 (e.g., SEQ ID NO:870 T242N, SEQ ID NO:220 G240N, or SEQ ID NO:220 T241N). In one embodiment, the variant is a nucleic acid molecule that has been codon optimized. In certain embodiments, the variant polypeptide is a chimeric polypeptide.

In certain embodiments, a nucleic acid molecule is contained within an expression vector and can be, for example, overexpressed. In certain embodiments, the aminotransferase or oxidoreductase polypeptide is immobilized on a solid support. In certain embodiments, the tryptophan or the MP is a substituted tryptophan or a substituted MP. A representative tryptophan is 6-chloro-D-tryptophan.

In another aspect, the invention provides methods of converting tryptophan to indole-3-pyruvate (or, alternatively, indole-3-pyruvate to tryptophan). Such methods typically include combining tryptophan (or indole-3-pyruvate) with a) one or more nucleic acid molecules chosen from the group consisting of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 865, 867, 869, 871, 873, 875, 877, 879, 881, 883, 885, 887, 889, 891, 893, 895, 897, 899, 901, 903, 905, 907, 909, 911, 913, 915, 917, 919, 921, 923, 925, 927, 929, 931, 933, 935, 937, 939, 941, 943, 945, 947, 949, 951, 953, 955, 957, 959, 969, 971, 973, and 975, wherein the one or more nucleic acid molecules encode polypeptides having D-aminotransferase (DAT) activity; b) a variant of a), wherein the variant encodes a polypeptide having DAT activity; c) a fragment of a) or b), wherein the fragment encodes a polypeptide having DAT activity; d) one or more polypeptides chosen from the group consisting of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 220 G240N, 220 T241N, SEQ ID NO:220 having one or more of the mutations shown in Table 43 or Table 52, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 866, 868, 870, 870 T242N, 872, 874, 876, 878, 880, 882, 884, 886, 888, 890, 892, 894, 896, 898, 900, 902, 904, 906, 908, 910, 912, 914, 916, 918, 920, 922, 924, 926, 928, 930, 932, 934, 936, 938, 940, 942, 944, 946, 948, 950, 952, 954, 956, 958, 960, 970, 972, 974, 976, 1069, 1070, 1071, 1072 and 1073, wherein the one or more polypeptides has DAT activity; e) a variant of d), wherein the variant has DAT activity; or f) a fragment of d) or e), wherein the fragment has DAT activity.

In still another aspect, the invention provides for methods of converting MP to monatin (or, alternatively, monatin to MP). Such methods generally include combining MP (or monatin) with a) one or more nucleic acid molecules chosen from the group consisting of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 865, 867, 869, 871, 873, 875, 877, 879, 881, 883, 885, 887, 889, 891, 893, 895, 897, 899, 901, 903, 905, 907, 909, 911, 913, 915, 917, 919, 921, 923, 925, 927, 929, 931, 933, 935, 937, 939, 941, 943, 945, 947, 949, 951, 953, 955, 957, 959, 969, 971, 973, and 975, wherein the one or more nucleic acid molecules encode polypeptides having DAT activity; b) a variant of a), wherein the variant encodes a polypeptide having DAT activity; c) a fragment of a) or b), wherein the fragment encodes a polypeptide having DAT activity; d) one or more polypeptides chosen from the group consisting of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 220 G240N, 220 T241N, SEQ ID NO:220 having one or more of the mutations shown in Table 43 or Table 52, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 866, 868, 870, 870 T242N, 872, 874, 876, 878, 880, 882, 884, 886, 888, 890, 892, 894, 896, 898, 900, 902, 904, 906, 908, 910, 912, 914, 916, 918, 920, 922, 924, 926, 928, 930, 932, 934, 936, 938, 940, 942, 944, 946, 948, 950, 952, 954, 956, 958, 960, 970, 972, 974, 976, 1069, 1070, 1071, 1072 and 1073, wherein the one or more polypeptides has DAT activity; e) a variant of d), wherein the variant has DAT activity; or f) a fragment of d) or e), wherein the fragment has DAT activity.

In certain embodiments, the nucleic acid molecule or polypeptide has a sequence selected from the group consisting of SEQ ID NO:945, 891, 893, 219, 175, 1063, 1065, and 1067. In certain embodiments, the polypeptide has a sequence that corresponds to a consensus sequence shown in SEQ ID NO:1069, 1070, 1071, 1072 or 1073. In some embodiments, the tryptophan or the MP is a substituted tryptophan or a substituted MP. A representative substituted tryptophan is 6-chloro-D-tryptophan.

In still another aspect, the invention provides methods of making monatin. Generally, such methods include contacting tryptophan, under conditions in which monatin is produced, with a C3 carbon source and a) one or more nucleic acid molecules chosen from the group consisting of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 865, 867, 869, 871, 873, 875, 877, 879, 881, 883, 885, 887, 889, 891, 893, 895, 897, 899, 901, 903, 905, 907, 909, 911, 913, 915, 917, 919, 921, 923, 925, 927, 929, 931, 933, 935, 937, 939, 941, 943, 945, 947, 949, 951, 953, 955, 957, 959, 969, 971, 973, and 975, wherein the one or more nucleic acid molecules encode polypeptides having D-aminotransferase (DAT) activity; b) a variant of a), wherein the variant encodes a polypeptide having DAT activity; c) a fragment of a) or b), wherein the fragment encodes a polypeptide having DAT activity; d) one or more polypeptides chosen from the group consisting of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 220 G240N, 220 T241N, SEQ ID NO:220 having one or more of the mutations shown in Table 43 or Table 52, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 866, 868, 870, 870 T242N, 872, 874, 876, 878, 880, 882, 884, 886, 888, 890, 892, 894, 896, 898, 900, 902, 904, 906, 908, 910, 912, 914, 916, 918, 920, 922, 924, 926, 928, 930, 932, 934, 936, 938, 940, 942, 944, 946, 948, 950, 952, 954, 956, 958, 960, 970, 972, 974, 976, 1069, 1070, 1071, 1072 and 1073, wherein the one or more polypeptides has DAT activity; e) a variant of d), wherein the variant has DAT activity; or f) a fragment of d) or e), wherein the fragment has DAT activity.

In some embodiments, the nucleic acid molecule is chosen from the group consisting of SEQ ID NO: 945, 891, 893, 219, 175, 1063, 1065, and 1067. In some embodiments, the C3 carbon source is selected from the group consisting of pyruvate, oxaloacetate, and serine. In some embodiments, the method further comprises adding or including a synthase/lyase (EC 4.1.2.- or 4.1.3.-) polypeptide. Representative synthase/lyase (EC 4.1.2.- or 4.1.3.-) polypeptides include aldolases such as KHG aldolases (EC 4.1.3.16) or HMG aldolases (EC 4.1.3.16).

In some embodiments of the above-indicated methods, the monatin produced is R,R monatin. In some embodiments, the monatin produced is S,R monatin. In certain embodiments, the tryptophan is a substituted tryptophan such as, for example, 6-chloro-D-tryptophan.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including definitions, will control.

The details of one or more embodiments of the invention are set forth in the accompanying drawings and the description below. Other features, objects, and advantages of the invention will be apparent from the drawings and detailed description, and from the claims.

DESCRIPTION OF DRAWINGS

FIG. 1 is an alignment of SEQ ID NO:894, 1066, 1064, and 1068.

FIG. 2 is an alignment of SEQ ID NO:870, 910, and several Bacillus sequences. Consensus sequences A and B (SEQ ID NO:1069 and 1070, respectively) directed toward the novel portions of SEQ ID NO:870 were developed from this alignment.

FIG. 3 is an alignment of SEQ ID NO:946, 894, 892, 220, 176, 1064, 1066, and 1068. Consensus sequences C, D and E (SEQ ID NO:1071, 1072 and 1073, respectively) were developed from this alignment.

FIG. 4 is a model of 3DAA-D-amino acid aminotransferase, with numbered residues indicating those sites selected for TMCA^(SM) evolution, as described in detail below.

Like reference symbols in the various drawings indicate like elements.

DETAILED DESCRIPTION

Disclosed herein are a number of different nucleic acid molecules encoding polypeptides having aminotransferase (AT) activity (e.g., transaminase activity). Specifically disclosed are a number of D-aminotransferases (DATs). DATs catalyze a transamination reaction (e.g., D-alanine+2-oxoglutarate<=>pyruvate+D-glutamate). Also provided are a number of different nucleic acid molecules encoding polypeptides having oxidoreductase activity (e.g., dehydrogenase activity). Oxidoreductases such as dehydrogenases catalyze an oxidation-reduction reaction (e.g., D-amino acid+H₂O+acceptor<=>a 2-oxo acid+NH₃+reduced acceptor). The nucleic acids or polypeptides disclosed herein can be used to convert tryptophan to indole-3-pyruvate and/or 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid (“monatin precursor” or “MP”) to monatin.

Isolated Nucleic Acid Molecules and Purified Polypeptides

The present invention is based, in part, on the identification of nucleic acid molecules encoding polypeptides having aminotransferase (AT) activity, herein referred to as “AT” nucleic acid molecules or polypeptides, where appropriate. The present invention also is based, in part, on the identification of nucleic acid molecules encoding polypeptides having oxidoreductase activity, herein referred to as “oxidoreductase” nucleic acid molecules or polypeptides, where appropriate.

Particular nucleic acid molecules described herein include the sequences shown in SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295, 297, 299, 301, 303, 305, 307, 309, 311, 313, 315, 317, 319, 321, 323, 325, 327, 329, 331, 333, 335, 337, 339, 341, 343, 345, 347, 349, 351, 353, 355, 357, 359, 361, 363, 365, 367, 369, 371, 373, 375, 377, 379, 381, 383, 385, 387, 389, 391, 393, 395, 397, 399, 401, 403, 405, 407, 409, 411, 413, 415, 417, 419, 421, 423, 425, 427, 429, 431, 433, 435, 437, 439, 441, 443, 445, 447, 449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469, 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499, 501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525, 527, 529, 531, 533, 535, 537, 539, 541, 543, 545, 547, 549, 551, 553, 555, 557, 559, 561, 563, 565, 567, 569, 571, 573, 575, 577, 579, 581, 583, 585, 587 589, 591, 593, 595, 597, 599, 601, 603, 605, 607, 609, 611, 613, 615, 617, 619, 621, 623, 625, 627, 629, 631, 633, 635, 637, 639, 641, 643, 645, 647, 649, 651, 653, 655, 657, 659, 661, 663, 665, 667, 669, 671, 673, 675, 677, 679, 681, 683, 685, 687, 689, 691, 693, 695, 697, 699, 701, 703, 705, 707, 709, 711, 713, 715, 716, 719, 721, 723, 725, 727, 729, 731, 733, 735, 737, 739, 741, 743, 745, 747, 749, 751, 753, 755, 757, 759, 761, 763, 765, 767, 769, 771, 773, 775, 777, 779, 781, 783, 785, 787, 789, 791, 793, 795, 797, 799, 801, 803, 805, 807, 809, 811, 813, 815, 817, 819, 821, 823, 825, 827, 829, 831, 833, 835, 837, 839, 841, 843, 845, 847, 849, 851, 853, 855, 857, 859, 861, 863, 865, 867, 869, 871, 873, 875, 877, 879, 881, 883, 885, 887, 889, 891, 893, 895, 897, 899, 901, 903, 905, 907, 909, 911, 913, 915, 917, 919, 921, 923, 925, 927, 929, 931, 933, 935, 937, 939, 941, 943, 945, 947, 949, 951, 953, 955, 957, 959, 961, 963, 965, 967, 969, 971, 973, and 975. As used herein, the term “nucleic acid molecule” can include DNA molecules and RNA molecules, analogs of DNA or RNA generated using nucleotide analogs. A nucleic acid molecule of the invention can be single-stranded or double-stranded, depending upon its intended use. Nucleic acid molecules of the invention include molecules that have at least 75% sequence identity (e.g., at least 80%, 85%, 90%, 95%, or 99% sequence identity) to any of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295, 297, 299, 301, 303, 305, 307, 309, 311, 313, 315, 317, 319, 321, 323, 325, 327, 329, 331, 333, 335, 337, 339, 341, 343, 345, 347, 349, 351, 353, 355, 357, 359, 361, 363, 365, 367, 369, 371, 373, 375, 377, 379, 381, 383, 385, 387, 389, 391, 393, 395, 397, 399, 401, 403, 405, 407, 409, 411, 413, 415, 417, 419, 421, 423, 425, 427, 429, 431, 433, 435, 437, 439, 441, 443, 445, 447, 449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469, 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499, 501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525, 527, 529, 531, 533, 535, 537, 539, 541, 543, 545, 547, 549, 551, 553, 555, 557, 559, 561, 563, 565, 567, 569, 571, 573, 575, 577, 579, 581, 583, 585, 587 589, 591, 593, 595, 597, 599, 601, 603, 605, 607, 609, 611, 613, 615, 617, 619, 621, 623, 625, 627, 629, 631, 633, 635, 637, 639, 641, 643, 645, 647, 649, 651, 653, 655, 657, 659, 661, 663, 665, 667, 669, 671, 673, 675, 677, 679, 681, 683, 685, 687, 689, 691, 693, 695, 697, 699, 701, 703, 705, 707, 709, 711, 713, 715, 716, 719, 721, 723, 725, 727, 729, 731, 733, 735, 737, 739, 741, 743, 745, 747, 749, 751, 753, 755, 757, 759, 761, 763, 765, 767, 769, 771, 773, 775, 777, 779, 781, 783, 785, 787, 789, 791, 793, 795, 797, 799, 801, 803, 805, 807, 809, 811, 813, 815, 817, 819, 821, 823, 825, 827, 829, 831, 833, 835, 837, 839, 841, 843, 845, 847, 849, 851, 853, 855, 857, 859, 861, 863, 865, 867, 869, 871, 873, 875, 877, 879, 881, 883, 885, 887, 889, 891, 893, 895, 897, 899, 901, 903, 905, 907, 909, 911, 913, 915, 917, 919, 921, 923, 925, 927, 929, 931, 933, 935, 937, 939, 941, 943, 945, 947, 949, 951, 953, 955, 957, 959, 961, 963, 965, 967, 969, 971, 973, and 975 and that have functional AT or oxidoreductase activity.

In calculating percent sequence identity, two sequences are aligned and the number of identical matches of nucleotides or amino acid residues between the two sequences is determined. The number of identical matches is divided by the length of the aligned region (i.e., the number of aligned nucleotides or amino acid residues) and multiplied by 100 to arrive at a percent sequence identity value. It will be appreciated that the length of the aligned region can be a portion of one or both sequences up to the full-length size of the shortest sequence. It will be appreciated that a single sequence can align differently with other sequences and hence, can have different percent sequence identity values over each aligned region. It is noted that the percent identity value is usually rounded to the nearest integer. For example, 78.1%, 78.2%, 78.3%, and 78.4% are rounded down to 78%, while 78.5%, 78.6%, 78.7%, 78.8%, and 78.9% are rounded up to 79%. It is also noted that the length of the aligned region is always an integer.

The alignment of two or more sequences to determine percent sequence identity is performed using the algorithm described by Altschul et al. (1997, Nucleic Acids Res., 25:3389-3402) as incorporated into BLAST (basic local alignment search tool) programs, available at ncbi.nlm.nih.gov on the World Wide Web. BLAST searches can be performed to determine percent sequence identity between an AT nucleic acid molecule described herein and any other sequence or portion thereof aligned using the Altschul et al. algorithm. BLASTN is the program used to align and compare the identity between nucleic acid sequences, while BLASTP is the program used to align and compare the identity between amino acid sequences. When utilizing BLAST programs to calculate the percent identity between a sequence of the invention and another sequence, the default parameters of the respective programs are used.

Nucleic acid molecules of the invention, for example, those between about 10 and about 50 nucleotides in length, can be used, under standard amplification conditions, to amplify an AT or oxidoreductase nucleic acid molecule. Amplification of an AT or oxidoreductase nucleic acid can be for the purpose of detecting the presence or absence of an AT or oxidoreductase nucleic acid molecule or for the purpose of obtaining (e.g., cloning) an AT or oxidoreductase nucleic acid molecule. As used herein, standard amplification conditions refer to the basic components of an amplification reaction mix, and cycling conditions that include multiple cycles of denaturing the template nucleic acid, annealing the oligonucleotide primers to the template nucleic acid, and extension of the primers by the polymerase to produce an amplification product (see, for example, U.S. Pat. Nos. 4,683,195; 4,683,202; 4,800,159; and 4,965,188). The basic components of an amplification reaction mix generally include, for example, each of the four deoxynucleoside triphosphates, (e.g., dATP, dCTP, dTTP, and dGTP, or analogs thereof), oligonucleotide primers, template nucleic acid, and a polymerase enzyme. Template nucleic acid is typically denatured at a temperature of at least about 90° C., and extension from primers is typically performed at a temperature of at least about 72° C. In addition, variations to the original PCR methods (e.g., anchor PCR, RACE PCR, or ligation chain reaction (LCR)) have been developed and are known in the art. See, for example, Landegran et al., 1988, Science, 241:1077-1080; and Nakazawa et al., 1994, Proc. Natl. Acad. Sci. USA, 91:360-364).

The annealing temperature can be used to control the specificity of amplification. The temperature at which primers anneal to template nucleic acid must be below the Tm of each of the primers, but high enough to avoid non-specific annealing of primers to the template nucleic acid. The Tm is the temperature at which half of the DNA duplexes have separated into single strands, and can be predicted for an oligonucleotide primer using the formula provided in section 11.46 of Sambrook et al. (1989, Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.). Non-specific amplification products are detected as bands on a gel that are not the size expected for the correct amplification product.

Nucleic acid molecules of the invention, for example, those between about 10 and several hundred nucleotides in length (up to several thousand nucleotides in length), can be used, under standard hybridization conditions, to hybridize to an AT or oxidoreductase nucleic acid molecule. Hybridization to an AT or oxidoreductase nucleic acid molecule can be for the purpose of detecting or obtaining an AT or oxidoreductase nucleic acid molecule. As used herein, standard hybridization conditions between nucleic acid molecules are discussed in detail in Sambrook et al. (1989, Molecular Cloning: A Laboratory Manual, 2^(nd) Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.; Sections 7.37-7.57, 9.47-9.57, 11.7-11.8, and 11.45-11.57). For oligonucleotide probes less than about 100 nucleotides, Sambrook et al. discloses suitable Southern blot conditions in Sections 11.45-11.46. The Tm between a sequence that is less than 100 nucleotides in length and a second sequence can be calculated using the formula provided in Section 11.46. Sambrook et al. additionally discloses prehybridization and hybridization conditions for a Southern blot that uses oligonucleotide probes greater than about 100 nucleotides (see Sections 9.47-9.52). Hybridizations with an oligonucleotide greater than 100 nucleotides generally are performed 15-25° C. below the Tm. The Tm between a sequence greater than 100 nucleotides in length and a second sequence can be calculated using the formula provided in Sections 9.50-9.51 of Sambrook et al. Additionally, Sambrook et al. recommends the conditions indicated in Section 9.54 for washing a Southern blot that has been probed with an oligonucleotide greater than about 100 nucleotides.

The conditions under which membranes containing nucleic acids are prehybridized and hybridized, as well as the conditions under which membranes containing nucleic acids are washed to remove excess and non-specifically bound probe can play a significant role in the stringency of the hybridization. For example, hybridization and washing may be carried out under conditions of low stringency, moderate stringency or high stringency. Such conditions are described, for example, in Sambrook et al. section 11.45-11.46. The conditions used to achieve a particular level of stringency will vary, depending on the nature of the nucleic acids being hybridized. For example, the length, degree of complementarity, nucleotide sequence composition (e.g., G/C vs. A/T nucleotide content) and nucleic acid type (e.g., RNA v. DNA) of the hybridizing regions of the nucleic acids can be considered in selecting hybridization conditions. For example, washing conditions can be made more stringent by decreasing the salt concentration in the wash solutions and/or by increasing the temperature at which the washes are performed.

The amount of hybridization can be quantitated directly on a membrane or from an autoradiograph using, for example, a PhosphorImager or a Densitometer (Molecular Dynamics, Sunnyvale, Calif.). It is understood by those of skill in the art that interpreting the amount of hybridization can be affected by, for example, the specific activity of the labeled oligonucleotide probe, the number of probe-binding sites on the target nucleic acid, and the amount of exposure of an autoradiograph or other detection medium. It will be readily appreciated that, although any number of hybridization, washing and detection conditions can be used to examine hybridization of a probe nucleic acid molecule to immobilized target nucleic acids, it is more important to examine hybridization of a probe to target nucleic acids under identical hybridization, washing, and detection conditions. Preferably, the target nucleic acids are on the same membrane. It can be appreciated by those of skill in the art that appropriate positive and negative controls should be performed with every set of amplification or hybridization reactions to avoid uncertainties related to contamination and/or non-specific annealing of oligonucleotide primers or probes.

Oligonucleotide primers or probes specifically anneal or hybridize to one or more AT or oxidoreductase nucleic acids. For amplification, a pair of oligonucleotide primers generally anneal to opposite strands of the template nucleic acid, and should be an appropriate distance from one another such that the polymerase can effectively polymerize across the region and such that the amplification product can be readily detected using, for example, electrophoresis. Oligonucleotide primers or probes can be designed using, for example, a computer program such as OLIGO (Molecular Biology Insights Inc., Cascade, Colo.) to assist in designing oligonucleotides. Typically, oligonucleotide primers are 10 to 30 or 40 or 50 nucleotides in length (e.g., 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides in length), but can be longer or shorter if appropriate amplification conditions are used.

Non-limiting representative pairs of oligonucleotide primers that were used to amplify D-aminotransferase (DAT) nucleic acid molecules are shown in Tables 2-8 (SEQ ID NOs:978-1062 and 1074-1083), Table 46 (SEQ ID NOs:1084-1103) and Table 54 (SEQ ID NOs:1104-1125). The sequences shown in SEQ ID NOs:978-1062 and 1074-1083 are non-limiting examples of oligonucleotide primers that can be used to amplify AT nucleic acid molecules. Oligonucleotides in accordance with the invention can be obtained by restriction enzyme digestion of an AT or oxidoreductase nucleic acid molecules or can be prepared by standard chemical synthesis and other known techniques.

As used herein, an “isolated” nucleic acid molecule is a nucleic acid molecule that is separated from other nucleic acid molecules that are usually associated with the isolated nucleic acid molecule. Thus, an “isolated” nucleic acid molecule includes, without limitation, a nucleic acid molecule that is free of sequences that naturally flank one or both ends of the nucleic acid in the genome of the organism from which the isolated nucleic acid is derived (e.g., a cDNA or genomic DNA fragment produced by PCR or restriction endonuclease digestion). Such an isolated nucleic acid molecule is generally introduced into a vector (e.g., a cloning vector, or an expression vector) for convenience of manipulation or to generate a fusion nucleic acid molecule. In addition, an isolated nucleic acid molecule can include an engineered nucleic acid molecule such as a recombinant or a synthetic nucleic acid molecule. A nucleic acid molecule existing among hundreds to millions of other nucleic acid molecules within, for example, a nucleic acid library (e.g., a cDNA, or genomic library) or a portion of a gel (e.g., agarose, or polyacrylamine) containing restriction-digested genomic DNA is not to be considered an isolated nucleic acid.

Isolated nucleic acid molecules described herein having AT or oxidoreductase activity can be obtained using techniques routine in the art, many of which are described in the Examples herein. For example, isolated nucleic acids within the scope of the invention can be obtained using any method including, without limitation, recombinant nucleic acid technology, the polymerase chain reaction (e.g., PCR, e.g., direct amplification or site-directed mutagenesis), and/or nucleic acid hybridization techniques (e.g., Southern blotting). General PCR techniques are described, for example in PCR Primer: A Laboratory Manual, Dieffenbach & Dveksler, Eds., Cold Spring Harbor Laboratory Press, 1995. Recombinant nucleic acid techniques include, for example, restriction enzyme digestion and ligation, which can be used to isolate an AT or oxidoreductase nucleic acid molecule as described herein. Isolated nucleic acids of the invention also can be chemically synthesized, either as a single nucleic acid molecule or as a series of oligonucleotides.

Techniques for the manipulation of nucleic acids, such as, e.g., subcloning, labeling probes (e.g., random-primer labeling using Klenow polymerase, nick translation, amplification), sequencing, hybridization, amplification and the like are well described in the scientific and patent literature, see, e.g., Sambrook et al., Eds., 1989, Molecular Cloning: A Laboratory Manual (2^(nd) Ed.), Vols 1-3, Cold Spring Harbor Laboratory; Current Protocols in Molecular Biology, 1997, Ausubel, Ed. John Wiley & Sons, Inc., New York; Laboratory Techniques in Biochemistry and Molecular Biology: Hybridization with Nucleic Acid Probes, Part I. Theory and Nucleic Acid Preparation, Tijssen, Ed. Elsevier, N.Y. (1993).

Purified AT or oxidoreductase polypeptides, as well as polypeptide fragments having AT or oxidoreductase activity, are within the scope of the invention. AT polypeptides refer to polypeptides that catalyze a reaction between an amino group and a keto acid. Specifically, a transamination reaction by a DAT involves removing an amino group from an amino acid leaving behind an alpha-keto acid, and transferring the amino group to the reactant alpha-keto acid, thereby converting the alpha-keto acid into an amino acid. The predicted amino acid sequences of AT polypeptides are shown in SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, SEQ ID NO:220 having one or more of the mutations shown in Table 43 or Table 52, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 866, 868, 870, 872, 874, 876, 878, 880, 882, 884, 886, 888, 890, 892, 894, 896, 898, 900, 902, 904, 906, 908, 910, 912, 914, 916, 918, 920, 922, 924, 926, 928, 930, 932, 934, 936, 938, 940, 942, 944, 946, 948, 950, 952, 954, 956, 958, 960, 970, 972, 974, and 976. An oxidoreductase polypeptide refers to a polypeptide that catalyzes an oxidation-reduction reaction, and the predicted amino acid sequences of oxidoreductase polypeptides are shown in SEQ ID NOs:252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 308, 310, 312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348, 350, 352, 354, 356, 358, 360, 362, 364, 366, 368, 370, 372, 374, 376, 378, 380, 382, 384, 386, 388, 390, 392, 394, 396, 398, 400, 402, 404, 406, 408, 410, 412, 414, 416, 418, 420, 422, 424, 426, 428, 430, 432, 434, 436, 438, 440, 442, 444, 446, 448, 450, 452, 454, 456, 458, 460, 462, 464, 466, 468, 470, 472, 474, 476, 478, 480, 482, 484, 486, 488, 490, 492, 494, 496, 498, 500, 502, 504, 506, 508, 510, 512, 514, 516, 518, 520, 522, 524, 526, 528, 530, 532, 534, 536, 538, 540, 542, 544, 546, 548, 550, 552, 554, 556, 558, 560, 562, 564, 566, 568, 570, 572, 574, 576, 578, 580, 582, 584, 586, 588, 590, 592, 594, 596, 598, 600, 602, 604, 606, 608, 610, 612, 614, 616, 618, 620, 622, 624, 626, 628, 630, 632, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, 684, 686, 688, 690, 692, 694, 696, 698, 700, 702, 704, 706, 708, 710, 712, 714, 716, 718, 720, 722, 724, 726, 728, 730, 732, 734, 736, 738, 740, 742, 744, 746, 748, 750, 752, 754, 756, 758, 760, 762, 764, 766, 768, 770, 772, 774, 776, 778, 780, 782, 784, 786, 788, 790, 792, 794, 796, 798, 800, 802, 804, 806, 808, 810, 812, 814, 816, 818, 820, 822, 824, 826, 828, 830, 832, 834, 836, 838, 840, 842, 844, 846, 848, 850, 852, 854, 856, 858, 860, 862, 864, 962, 964, 966 and 968.

The term “purified” polypeptide as used herein refers to a polypeptide that has been separated from cellular components that naturally accompany it. Typically, a polypeptide is considered “purified” when it is at least partially free from the proteins and naturally occurring molecules with which it is naturally associated. The extent of enrichment or purity of an AT or oxidoreductase polypeptide can be measured using any appropriate method, e.g., column chromatography, polyacrylamide gel electrophoresis, or HPLC analysis.

The invention also provides for AT and oxidoreductase polypeptides that differ in sequence from SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, SEQ ID NO:220 having one or more of the mutations shown in Table 43 or Table 52, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 308, 310, 312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348, 350, 352, 354, 356, 358, 360, 362, 364, 366, 368, 370, 372, 374, 376, 378, 380, 382, 384, 386, 388, 390, 392, 394, 396, 398, 400, 402, 404, 406, 408, 410, 412, 414, 416, 418, 420, 422, 424, 426, 428, 430, 432, 434, 436, 438, 440, 442, 444, 446, 448, 450, 452, 454, 456, 458, 460, 462, 464, 466, 468, 470, 472, 474, 476, 478, 480, 482, 484, 486, 488, 490, 492, 494, 496, 498, 500, 502, 504, 506, 508, 510, 512, 514, 516, 518, 520, 522, 524, 526, 528, 530, 532, 534, 536, 538, 540, 542, 544, 546, 548, 550, 552, 554, 556, 558, 560, 562, 564, 566, 568, 570, 572, 574, 576, 578, 580, 582, 584, 586, 588, 590, 592, 594, 596, 598, 600, 602, 604, 606, 608, 610, 612, 614, 616, 618, 620, 622, 624, 626, 628, 630, 632, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, 684, 686, 688, 690, 692, 694, 696, 698, 700, 702, 704, 706, 708, 710, 712, 714, 716, 718, 720, 722, 724, 726, 728, 730, 732, 734, 736, 738, 740, 742, 744, 746, 748, 750, 752, 754, 756, 758, 760, 762, 764, 766, 768, 770, 772, 774, 776, 778, 780, 782, 784, 786, 788, 790, 792, 794, 796, 798, 800, 802, 804, 806, 808, 810, 812, 814, 816, 818, 820, 822, 824, 826, 828, 830, 832, 834, 836, 838, 840, 842, 844, 846, 848, 850, 852, 854, 856, 858, 860, 862, 864, 866, 868, 870, 872, 874, 876, 878, 880, 882, 884, 886, 888, 890, 892, 894, 896, 898, 900, 902, 904, 906, 908, 910, 912, 914, 916, 918, 920, 922, 924, 926, 928, 930, 932, 934, 936, 938, 940, 942, 944, 946, 948, 950, 952, 954, 956, 958, 960, 962, 964, 966, 968, 970, 972, 974, and 976. For example, the skilled artisan will appreciate that changes can be introduced into an AT or oxidoreductase polypeptide (e.g., SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, SEQ ID NO:220 having one or more of the mutations shown in Table 43 or Table 52, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 308, 310, 312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348, 350, 352, 354, 356, 358, 360, 362, 364, 366, 368, 370, 372, 374, 376, 378, 380, 382, 384, 386, 388, 390, 392, 394, 396, 398, 400, 402, 404, 406, 408, 410, 412, 414, 416, 418, 420, 422, 424, 426, 428, 430, 432, 434, 436, 438, 440, 442, 444, 446, 448, 450, 452, 454, 456, 458, 460, 462, 464, 466, 468, 470, 472, 474, 476, 478, 480, 482, 484, 486, 488, 490, 492, 494, 496, 498, 500, 502, 504, 506, 508, 510, 512, 514, 516, 518, 520, 522, 524, 526, 528, 530, 532, 534, 536, 538, 540, 542, 544, 546, 548, 550, 552, 554, 556, 558, 560, 562, 564, 566, 568, 570, 572, 574, 576, 578, 580, 582, 584, 586, 588, 590, 592, 594, 596, 598, 600, 602, 604, 606, 608, 610, 612, 614, 616, 618, 620, 622, 624, 626, 628, 630, 632, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, 684, 686, 688, 690, 692, 694, 696, 698, 700, 702, 704, 706, 708, 710, 712, 714, 716, 718, 720, 722, 724, 726, 728, 730, 732, 734, 736, 738, 740, 742, 744, 746, 748, 750, 752, 754, 756, 758, 760, 762, 764, 766, 768, 770, 772, 774, 776, 778, 780, 782, 784, 786, 788, 790, 792, 794, 796, 798, 800, 802, 804, 806, 808, 810, 812, 814, 816, 818, 820, 822, 824, 826, 828, 830, 832, 834, 836, 838, 840, 842, 844, 846, 848, 850, 852, 854, 856, 858, 860, 862, 864, 866, 868, 870, 872, 874, 876, 878, 880, 882, 884, 886, 888, 890, 892, 894, 896, 898, 900, 902, 904, 906, 908, 910, 912, 914, 916, 918, 920, 922, 924, 926, 928, 930, 932, 934, 936, 938, 940, 942, 944, 946, 948, 950, 952, 954, 956, 958, 960, 962, 964, 966, 968, 970, 972, 974, and 976) or in an AT or oxidoreductase nucleic acid molecule (e.g., SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295, 297, 299, 301, 303, 305, 307, 309, 311, 313, 315, 317, 319, 321, 323, 325, 327, 329, 331, 333, 335, 337, 339, 341, 343, 345, 347, 349, 351, 353, 355, 357, 359, 361, 363, 365, 367, 369, 371, 373, 375, 377, 379, 381, 383, 385, 387, 389, 391, 393, 395, 397, 399, 401, 403, 405, 407, 409, 411, 413, 415, 417, 419, 421, 423, 425, 427, 429, 431, 433, 435, 437, 439, 441, 443, 445, 447, 449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469, 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499, 501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525, 527, 529, 531, 533, 535, 537, 539, 541, 543, 545, 547, 549, 551, 553, 555, 557, 559, 561, 563, 565, 567, 569, 571, 573, 575, 577, 579, 581, 583, 585, 587 589, 591, 593, 595, 597, 599, 601, 603, 605, 607, 609, 611, 613, 615, 617, 619, 621, 623, 625, 627, 629, 631, 633, 635, 637, 639, 641, 643, 645, 647, 649, 651, 653, 655, 657, 659, 661, 663, 665, 667, 669, 671, 673, 675, 677, 679, 681, 683, 685, 687, 689, 691, 693, 695, 697, 699, 701, 703, 705, 707, 709, 711, 713, 715, 716, 719, 721, 723, 725, 727, 729, 731, 733, 735, 737, 739, 741, 743, 745, 747, 749, 751, 753, 755, 757, 759, 761, 763, 765, 767, 769, 771, 773, 775, 777, 779, 781, 783, 785, 787, 789, 791, 793, 795, 797, 799, 801, 803, 805, 807, 809, 811, 813, 815, 817, 819, 821, 823, 825, 827, 829, 831, 833, 835, 837, 839, 841, 843, 845, 847, 849, 851, 853, 855, 857, 859, 861, 863, 865, 867, 869, 871, 873, 875, 877, 879, 881, 883, 885, 887, 889, 891, 893, 895, 897, 899, 901, 903, 905, 907, 909, 911, 913, 915, 917, 919, 921, 923, 925, 927, 929, 931, 933, 935, 937, 939, 941, 943, 945, 947, 949, 951, 953, 955, 957, 959, 961, 963, 965, 967, 969, 971, 973, and 975), thereby leading to changes in the amino acid sequence of the encoded polypeptide. AT and oxidoreductase polypeptides that differ in sequence from SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, SEQ ID NO:220 having one or more of the mutations shown in Table 43 or Table 52, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 308, 310, 312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348, 350, 352, 354, 356, 358, 360, 362, 364, 366, 368, 370, 372, 374, 376, 378, 380, 382, 384, 386, 388, 390, 392, 394, 396, 398, 400, 402, 404, 406, 408, 410, 412, 414, 416, 418, 420, 422, 424, 426, 428, 430, 432, 434, 436, 438, 440, 442, 444, 446, 448, 450, 452, 454, 456, 458, 460, 462, 464, 466, 468, 470, 472, 474, 476, 478, 480, 482, 484, 486, 488, 490, 492, 494, 496, 498, 500, 502, 504, 506, 508, 510, 512, 514, 516, 518, 520, 522, 524, 526, 528, 530, 532, 534, 536, 538, 540, 542, 544, 546, 548, 550, 552, 554, 556, 558, 560, 562, 564, 566, 568, 570, 572, 574, 576, 578, 580, 582, 584, 586, 588, 590, 592, 594, 596, 598, 600, 602, 604, 606, 608, 610, 612, 614, 616, 618, 620, 622, 624, 626, 628, 630, 632, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, 684, 686, 688, 690, 692, 694, 696, 698, 700, 702, 704, 706, 708, 710, 712, 714, 716, 718, 720, 722, 724, 726, 728, 730, 732, 734, 736, 738, 740, 742, 744, 746, 748, 750, 752, 754, 756, 758, 760, 762, 764, 766, 768, 770, 772, 774, 776, 778, 780, 782, 784, 786, 788, 790, 792, 794, 796, 798, 800, 802, 804, 806, 808, 810, 812, 814, 816, 818, 820, 822, 824, 826, 828, 830, 832, 834, 836, 838, 840, 842, 844, 846, 848, 850, 852, 854, 856, 858, 860, 862, 864, 866, 868, 870, 872, 874, 876, 878, 880, 882, 884, 886, 888, 890, 892, 894, 896, 898, 900, 902, 904, 906, 908, 910, 912, 914, 916, 918, 920, 922, 924, 926, 928, 930, 932, 934, 936, 938, 940, 942, 944, 946, 948, 950, 952, 954, 956, 958, 960, 962, 964, 966, 968, 970, 972, 974, and 976 and that retain aminotransferase and oxidoreductase activity, respectively, readily can be identified by screening methods routinely used in the art.

For example, changes can be introduced into an AT or oxidoreductase nucleic acid coding sequence that lead to conservative and/or non-conservative amino acid substitutions at one or more amino acid residues in the encoded AT or oxidoreductase polypeptide. Changes in nucleic acid sequences can be generated by standard techniques, such as site-directed mutagenesis, PCR-mediated mutagenesis of a nucleic acid encoding such a polypeptide, or directed evolution. In addition, changes in the polypeptide sequence can be introduced randomly along all or part of the AT or oxidoreductase polypeptide, such as by saturation mutagenesis of the corresponding nucleic acid. Alternatively, changes can be introduced into a nucleic acid or polypeptide sequence by chemically synthesizing a nucleic acid molecule or polypeptide having such changes.

A “conservative amino acid substitution” is one in which one amino acid residue is replaced with a different amino acid residue having a similar side chain. Similarity between amino acid residues has been assessed in the art. For example, Dayhoff et al. (1978, in Atlas of Protein Sequence and Structure, 5(Suppl. 3):345-352) provides frequency tables for amino acid substitutions that can be employed as a measure of amino acid similarity. Examples of conservative substitutions include, for example, replacement of an aliphatic amino acid with another aliphatic amino acid; replacement of a serine with a threonine or vice versa; replacement of an acidic residue with another acidic residue; replacement of a residue bearing an amide group with another residue bearing an amide group; exchange of a basic residue with another basic residue; or replacement of an aromatic residue with another aromatic residue. A non-conservative substitution is one in which an amino acid residue is replaced with an amino acid residue that does not have a similar side chain.

Changes in a nucleic acid can be introduced using one or more mutagens. Mutagens include, without limitation, ultraviolet light, gamma irradiation, or chemical mutagens (e.g., mitomycin, nitrous acid, photoactivated psoralens, sodium bisulfite, nitrous acid, hydroxylamine, hydrazine or formic acid). Other mutagens are analogues of nucleotide precursors, e.g., nitrosoguanidine, 5-bromouracil, 2-aminopurine, or acridine. Intercalating agents such as proflavine, acriflavine, quinacrine and the like can also be used.

Changes also can be introduced into an AT or oxidoreductase nucleic acid and/or polypeptide by methods such as error-prone PCR, shuffling, oligonucleotide-directed mutagenesis, assembly PCR, sexual PCR mutagenesis, in vivo mutagenesis, cassette mutagenesis, recursive ensemble mutagenesis, exponential ensemble mutagenesis, gene reassembly (e.g., GeneReassembly, see, e.g., U.S. Pat. No. 6,537,776), Gene Site Saturation Mutagenesis (GSSM), synthetic ligation reassembly (SLR), or a combination thereof. Changes also can be introduced into polypeptides by methods such as recombination, recursive sequence recombination, phosphothioate-modified DNA mutagenesis, uracil-containing template mutagenesis, gapped duplex mutagenesis, point mismatch repair mutagenesis, repair-deficient host strain mutagenesis, chemical mutagenesis, radiogenic mutagenesis, deletion mutagenesis, restriction-selection mutagenesis, restriction-purification mutagenesis, artificial gene synthesis, ensemble mutagenesis, chimeric nucleic acid multimer creation, or any combination thereof.

An AT or oxidoreductase nucleic acid can be codon optimized if so desired. For example, a non-preferred or a less preferred codon can be identified and replaced with a preferred or neutrally used codon encoding the same amino acid as the replaced codon. A preferred codon is a codon over-represented in coding sequences in genes in the host cell and a non-preferred or less preferred codon is a codon under-represented in coding sequences in genes in the host cell, thereby modifying the nucleic acid to increase its expression in a host cell. An AT or oxidoreductase nucleic acid can be optimized for particular codon usage from any host cell (e.g., any of the host cells described herein). See, for example, U.S. Pat. No. 5,795,737 for a representative description of codon optimization. In addition to codon optimization, a nucleic acid can undergo directed evolution. See, for example, U.S. Pat. No. 6,361,974.

Other changes also are within the scope of this disclosure. For example, one, two, three, four or more amino acids can be removed from the carboxy- and/or amino-terminal ends of an aminotransferase or oxidoreductase polypeptide without significantly altering the biological activity. In addition, one or more amino acids can be changed to increase or decrease the pl of a polypeptide. In some embodiments, a residue can be changed to, for example, a glutamate. Also provided are chimeric aminotransferase or oxidoreductase polypeptides. For example, a chimeric AT or oxidoreductase polypeptide can include portions of different binding or catalytic domains. Methods of recombining different domains from different polypeptides and screening the resultant chimerics to find the best combination for a particular application or substrate are routine in the art.

One particular change in sequence that was exemplified herein involves the residue corresponding to residue 243 in a DAT from ATCC Accession No. 4978 (DAT 4978). In one instance, the polypeptide sequence of the SEQ ID NO:870 DAT was aligned with DAT 4978 and the residue in SEQ ID NO:870 that aligns with position 243 in DAT 4978 was identified (residue 242) and changed from Thr to Asn (SEQ ID NO:870 T242N). In another instance, the polypeptide sequence of the SEQ ID NO:220 DAT was aligned with DAT 4978 and the residue in SEQ ID NO:220 that aligns with position 243 in DAT 4978 was identified (either residue 240 or 241) and changed from Gly to Asn or Thr to Asn, respectively (SEQ ID NO:220 G240N and SEQ ID NO:220 T241N). Those of skill in the art can readily identify the residue that corresponds to residue 243 from DAT 4978 in any of the DATs disclosed herein and introduce a change into the polypeptide sequence at that particular residue. A number of additional DAT mutants were made and are listed in Tables 43 and 52.

It is noted that SEQ ID NO:894 is a novel DAT, for which the closest sequence in the public databases exhibits only 60% sequence identity to the SEQ ID NO:894 polypeptide. Therefore, polypeptides of the invention include sequences that have at least, for example, 65% sequence identity (e.g., at least 70%, 75%, 80%, 85%, 90%, 95%, or 99% sequence identity) to SEQ ID NO:894 and that have functional DAT activity. In addition, SEQ ID NO:870 also is a novel DAT and has 76% sequence identity to a Bacillus DAT polypeptide and 69% sequence identity to a B. sphaericus DAT polypeptide. Therefore, polypeptides of the invention include sequences having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, or 99% sequence identity) to SEQ ID NO:870 and that have DAT activity. Further, SEQ ID NO:220 is a novel DAT that has 62% sequence identity to a DAT polypeptide from C. beijerinckii. Therefore, polypeptides of the invention include sequences having at least 65% sequence identity (e.g., at least 70%, 75%, 80%, 85%, 90%, 95%, or 99% sequence identity) to SEQ ID NO:220.

In one instance, SEQ ID NO:870 and 910 were aligned with published DATs and a consensus sequence was determined. The consensus sequence for SEQ ID NO:870-like DAT polypeptides that make it unique from the rest of the group of Bacillus-like DAT polypeptides is shown in SEQ ID NO:1069 (consensus sequence A). In another instance, SEQ ID NO:176, 220, 892, 894 and 946 were aligned and a consensus sequence was determined. The consensus sequence for this group of DATs is shown in SEQ ID NO:1071 (consensus sequence C). SEQ ID NO:1070 (consensus sequence B) represents a slightly more conservative consensus sequence relative to SEQ ID NO:1069 (consensus sequence A), while SEQ ID NO:1072 (consensus sequence D) and SEQ ID NO:1073 (consensus sequence E) correspond to slightly more conservative consensus sequences relative to SEQ ID NO:1071 (consensus sequence C). It is expected that polypeptides having a consensus sequence that corresponds to consensus sequence A, B, C, D or E as disclosed herein would exhibit DAT activity.

A fragment of an aminotransferase and oxidoreductase nucleic acid or polypeptide refers to a portion of a full-length aminotransferase and oxidoreductase nucleic acid or polypeptide. As used herein, “functional fragments” are those fragments of an aminotransferase or oxidoreductase polypeptide that retain the respective enzymatic activity. “Functional fragments” also refer to fragments of an aminotransferase or oxidoreductase nucleic acid that encode a polypeptide that retains the respective anzymatic activity. For example, functional fragments can be used in in vitro or in vivo reactions to catalyze transaminase or oxidation-reduction reactions, respectively.

AT or oxidoreductase polypeptides can be obtained (e.g., purified) from natural sources (e.g., a biological sample) by known methods such as DEAE ion exchange, gel filtration, and hydroxyapatite chromatography. Natural sources include, but are not limited to, microorganisms such as bacteria and yeast. A purified AT or oxidoreductase polypeptide also can be obtained, for example, by cloning and expressing an AT or oxidoreductase nucleic acid (e.g., SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295, 297, 299, 301, 303, 305, 307, 309, 311, 313, 315, 317, 319, 321, 323, 325, 327, 329, 331, 333, 335, 337, 339, 341, 343, 345, 347, 349, 351, 353, 355, 357, 359, 361, 363, 365, 367, 369, 371, 373, 375, 377, 379, 381, 383, 385, 387, 389, 391, 393, 395, 397, 399, 401, 403, 405, 407, 409, 411, 413, 415, 417, 419, 421, 423, 425, 427, 429, 431, 433, 435, 437, 439, 441, 443, 445, 447, 449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469, 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499, 501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525, 527, 529, 531, 533, 535, 537, 539, 541, 543, 545, 547, 549, 551, 553, 555, 557, 559, 561, 563, 565, 567, 569, 571, 573, 575, 577, 579, 581, 583, 585, 587 589, 591, 593, 595, 597, 599, 601, 603, 605, 607, 609, 611, 613, 615, 617, 619, 621, 623, 625, 627, 629, 631, 633, 635, 637, 639, 641, 643, 645, 647, 649, 651, 653, 655, 657, 659, 661, 663, 665, 667, 669, 671, 673, 675, 677, 679, 681, 683, 685, 687, 689, 691, 693, 695, 697, 699, 701, 703, 705, 707, 709, 711, 713, 715, 716, 719, 721, 723, 725, 727, 729, 731, 733, 735, 737, 739, 741, 743, 745, 747, 749, 751, 753, 755, 757, 759, 761, 763, 765, 767, 769, 771, 773, 775, 777, 779, 781, 783, 785, 787, 789, 791, 793, 795, 797, 799, 801, 803, 805, 807, 809, 811, 813, 815, 817, 819, 821, 823, 825, 827, 829, 831, 833, 835, 837, 839, 841, 843, 845, 847, 849, 851, 853, 855, 857, 859, 861, 863, 865, 867, 869, 871, 873, 875, 877, 879, 881, 883, 885, 887, 889, 891, 893, 895, 897, 899, 901, 903, 905, 907, 909, 911, 913, 915, 917, 919, 921, 923, 925, 927, 929, 931, 933, 935, 937, 939, 941, 943, 945, 947, 949, 951, 953, 955, 957, 959, 961, 963, 965, 967, 969, 971, 973, and 975) and purifying the resultant polypeptide using, for example, any of the known expression systems including, but not limited to, glutathione S-transferase (GST), pGEX (Pharmacia Biotech Inc), pMAL (New England Biolabs, Beverly, Mass.) or pRIT5 (Pharmacia, Piscataway, N.J.)). In addition, a purified AT or oxidoreductase polypeptide can be obtained by chemical synthesis using, for example, solid-phase synthesis techniques (see e.g., Roberge, 1995, Science, 269:202; Merrifield, 1997, Methods Enzymol., 289:3-13).

A purified AT or oxidoreductase polypeptide or a fragment thereof can be used as an immunogen to generate polyclonal or monoclonal antibodies that have specific binding affinity for one or more AT or oxidoreductase polypeptides. Such antibodies can be generated using standard techniques that are used routinely in the art. Full-length AT or oxidoreductase polypeptides or, alternatively, antigenic fragments of AT or oxidoreductase polypeptides can be used as immunogens. An antigenic fragment of an AT or oxidoreductase polypeptide usually includes at least 8 (e.g., 10, 15, 20, or 30) amino acid residues of an AT or oxidoreductase polypeptide (e.g., having the sequence shown in SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, SEQ ID NO:220 having one or more of the mutations shown in Table 43 or Table 52, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 308, 310, 312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348, 350, 352, 354, 356, 358, 360, 362, 364, 366, 368, 370, 372, 374, 376, 378, 380, 382, 384, 386, 388, 390, 392, 394, 396, 398, 400, 402, 404, 406, 408, 410, 412, 414, 416, 418, 420, 422, 424, 426, 428, 430, 432, 434, 436, 438, 440, 442, 444, 446, 448, 450, 452, 454, 456, 458, 460, 462, 464, 466, 468, 470, 472, 474, 476, 478, 480, 482, 484, 486, 488, 490, 492, 494, 496, 498, 500, 502, 504, 506, 508, 510, 512, 514, 516, 518, 520, 522, 524, 526, 528, 530, 532, 534, 536, 538, 540, 542, 544, 546, 548, 550, 552, 554, 556, 558, 560, 562, 564, 566, 568, 570, 572, 574, 576, 578, 580, 582, 584, 586, 588, 590, 592, 594, 596, 598, 600, 602, 604, 606, 608, 610, 612, 614, 616, 618, 620, 622, 624, 626, 628, 630, 632, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, 684, 686, 688, 690, 692, 694, 696, 698, 700, 702, 704, 706, 708, 710, 712, 714, 716, 718, 720, 722, 724, 726, 728, 730, 732, 734, 736, 738, 740, 742, 744, 746, 748, 750, 752, 754, 756, 758, 760, 762, 764, 766, 768, 770, 772, 774, 776, 778, 780, 782, 784, 786, 788, 790, 792, 794, 796, 798, 800, 802, 804, 806, 808, 810, 812, 814, 816, 818, 820, 822, 824, 826, 828, 830, 832, 834, 836, 838, 840, 842, 844, 846, 848, 850, 852, 854, 856, 858, 860, 862, 864, 866, 868, 870, 872, 874, 876, 878, 880, 882, 884, 886, 888, 890, 892, 894, 896, 898, 900, 902, 904, 906, 908, 910, 912, 914, 916, 918, 920, 922, 924, 926, 928, 930, 932, 934, 936, 938, 940, 942, 944, 946, 948, 950, 952, 954, 956, 958, 960, 962, 964, 966, 968, 970, 972, 974, and 976), and encompasses an epitope of an AT or oxidoreductase polypeptide such that an antibody (e.g., polyclonal or monoclonal; chimeric or humanized) raised against the antigenic fragment has specific binding affinity for one or more AT or oxidoreductase polypeptides.

Polypeptides can be detected and quantified by any method known in the art including, but not limited to, nuclear magnetic resonance (NMR), spectrophotometry, radiography (protein radiolabeling), electrophoresis, capillary electrophoresis, high performance liquid chromatography (HPLC), thin layer chromatography (TLC), hyperdiffusion chromatography, various immunological methods, e.g. immunoprecipitation, immunodiffusion, immuno-electrophoresis, radioimmunoassays (RIAs), enzyme-linked immunosorbent assays (ELISAs), immuno-fluorescent assays, gel electrophoresis (e.g., SDS-PAGE), staining with antibodies, fluorescent activated cell sorter (FACS), pyrolysis mass spectrometry, Fourier-Transform Infrared Spectrometry, Raman spectrometry, GC-MS, and LC-Electrospray and cap-LC-tandem-electrospray mass spectrometries, and the like. Novel bioactivities can also be screened using methods, or variations thereof, described in U.S. Pat. No. 6,057,103. Furthermore, one or more, or, all the polypeptides of a cell can be measured using a protein array.

Methods of Using DAT or Oxidoreductase Nucleic Acids and Polypeptides

The AT or oxidoreductase polypeptides or the AT or oxidoreductase nucleic acids encoding such AT and oxidoreductase polypeptides, respectively, can be used to facilitate the conversion of tryptophan to indole-3-pyruvate and/or to facilitate the conversion of MP to monatin (or the reverse reaction). It is noted that the reactions described herein are not limited to any particular method, unless otherwise stated. The reactions disclosed herein can take place, for example, in vivo, in vitro, or a combination thereof.

Constructs containing AT or oxidoreductase nucleic acid molecules are provided. Constructs, including expression vectors, suitable for expressing an AT or oxidoreductase polypeptide are commercially available and/or readily produced by recombinant DNA technology methods routine in the art. Representative constructs or vectors include, without limitation, replicons (e.g., RNA replicons, bacteriophages), autonomous self-replicating circular or linear DNA or RNA, a viral vector (e.g., an adenovirus vector, a retroviral vector or an adeno-associated viral vector), a plasmid, a phage, a phagemid, a cosmid, a fosmid, a bacteriophage or an artificial chromosome. The cloning vehicle can comprise an artificial chromosome comprising a bacterial artificial chromosome (BAC), a bacteriophage P1-derived vector (PAC), a yeast artificial chromosome (YAC), or a mammalian artificial chromosome (MAC). Exemplary vectors include, without limitation, pBR322 (ATCC 37017), pKK223-3, pSVK3, pBPV, pMSG, and pSVL (Pharmacia Fine Chemicals, Uppsala, Sweden), GEM1 (Promega Biotec, Madison, Wis., USA) pQE70, pQE60, pQE-9 (Qiagen), pD10, psiX174 pBluescript II KS, pNH8A, pNH16a, pNH18A, pNH46A, pSV2CAT, pOG44, pXT1, pSG (Stratagene), ptrc99a, pKK223-3, pKK233-3, DR540, pRIT5 (Pharmacia), pKK232-8 and pCM7. See, also, U.S. Pat. No. 5,217,879 for a description of representative plasmids, viruses, and the like.

A vector or construct containing an AT or oxidoreductase nucleic acid molecule can have elements necessary for expression operably linked to the AT or oxidoreductase nucleic acid. Elements necessary for expression include nucleic acid sequences that direct and regulate expression of nucleic acid coding sequences. One example of an element necessary for expression is a promoter sequence. Promoter sequences are sequences that are capable of driving transcription of a coding sequence. A promoter sequence can be, for example, an AT or oxidoreductase promoter sequence, or a non-AT or non-oxidoreductase promoter sequence. Non-AT and non-oxidoreductase promoters include, for example, bacterial promoters such as lacZ, T3, T7, gpt, lambda PR, lambdaPL and trp as well as eukaryotic promoters such as CMV immediate early, HSV thymidine kinase, early and late SV40, LTRs from retrovirus, and mouse metallothionein I. Promoters also can be, for example, constitutive, inducible, and/or tissue-specific. A representative constitutive promoter is the CaMV 35S; representative inducible promoters include, for example, arabinose, tetracycline-inducible and salicylic acid-responsive promoters.

Additional elements necessary for expression can include introns, enhancer sequences (e.g., an SV40 enhancer), response elements, or inducible elements that modulate expression of a nucleic acid. Elements necessary for expression can include a leader or signal sequence. See, for example, SEQ ID NO:156, which is a DAT polypeptide having a leader sequence. Elements necessary for expression also can include, for example, a ribosome binding site for translation initiation, splice donor and acceptor sites, and a transcription terminator. Elements necessary for expression can be of bacterial, yeast, insect, mammalian, or viral origin, and vectors or constructs can contain a combination of elements from different origins. Elements necessary for expression are described, for example, in Goeddel, 1990, Gene Expression Technology: Methods in Enzymology, 185, Academic Press, San Diego, Calif.

A vector or construct as described herein further can include sequences such as those encoding a selectable marker (e.g., genes encoding dihydrofolate reductase or genes conferring neomycin resistance for eukaryotic cells; genes conferring tetracycline or ampicillin resistance for E. coli; and the gene encoding TRP1 for S. cerevisiae), sequences that can be used in purification of an AT or oxidoreductase polypeptide (e.g., 6×His tag), and one or more sequences involved in replication of the vector or construct (e.g., origins of replication). In addition, a vector or construct can contain, for example, one or two regions that have sequence homology for integrating the vector or construct. Vectors and constructs for genomic integration are well known in the art.

As used herein, operably linked means that a promoter and/or other regulatory element(s) are positioned in a vector or construct relative to an AT or oxidoreductase nucleic acid in such a way as to direct or regulate expression of the AT or oxidoreductase nucleic acid. Generally, promoter and other elements necessary for expression that are operably linked to a transcribed sequence are physically contiguous to the transcribed sequence, i.e., they are cis-acting. Some transcriptional regulatory sequences such as enhancers, however, need not be physically contiguous or located in close proximity to the coding sequences whose expression they enhance.

Also provided are host cells. Host cells generally contain a nucleic acid sequence of the invention, e.g., a sequence encoding an AT or an oxidoreductase, or a vector or construct as described herein. The host cell may be any of the host cells familiar to those skilled in the art such as prokaryotic cells or eukaryotic cells including bacterial cells, fungal cells, yeast cells, mammalian cells, insect cells, or plant cells. Exemplary bacterial cells include any species within the genera Escherichia, Bacillus, Streptomyces, Salmonella, Pseudomonas and Staphylococcus, including, e.g., E. coli, L. lactis, B. subtilis, B. cereus, S. typhimurium, P. fluorescens. Exemplary fungal cells include any species of Aspergillus, and exemplary yeast cells include any species of Pichia, Saccharomyces, Schizosaccharomyces, or Schwanniomyces, including P. pastoris, S. cerevisiae, or S. pombe. Exemplary insect cells include any species of Spodoptera or Drosophila, including Drosophila S2 and Spodoptera Sf9. Exemplary animal cells include CHO, COS, Bowes melanoma, C127, 3T3, HeLa and BHK cell lines. See, for example, Gluzman, 1981, Cell, 23:175. The selection of an appropriate host is within the abilities of those skilled in the art.

Techniques for introducing nucleic acid into a wide variety of cells are well known and described in the technical and scientific literature. A vector or construct can be introduced into host cells using any of a variety of techniques, including transformation, transfection, transduction, viral infection, gene guns, or Ti-mediated gene transfer. Particular methods include calcium phosphate transfection, DEAE-Dextran mediated transfection, lipofection, or electroporation (Davis et al., 1986, Basic Methods in Molecular Biology). Exemplary methods include CaPO₄ precipitation, liposome fusion, lipofection (e.g., LIPOFECTIN™), electroporation, viral infection, etc. The AT or oxidoreductase nucleic acids may stably integrate into the genome of the host cell (for example, with retroviral introduction) or may exist either transiently or stably in the cytoplasm (i.e. through the use of traditional plasmids, utilizing standard regulatory sequences, selection markers, etc.).

The content of host cells usually is harvested by centrifugation, disrupted by physical or chemical means, and the resulting crude extract retained for further purification. Microbial cells employed for expression of proteins can be disrupted by any convenient method, including freeze-thaw cycling, sonication, mechanical disruption, or the use of cell lysing agents. Such methods are well known to those skilled in the art. The expressed polypeptide or fragment thereof can be recovered and purified from cell cultures by methods including, but not limited to, precipitation (e.g., ammonium sulfate or ethanol), acid extraction, chromatography (e.g., anion or cation exchange, phosphocellulose, hydrophobic interaction, affinity, hydroxylapatite and lectin). If desired, high performance liquid chromatography (HPLC) can be employed for final purification steps.

Cell-free translation systems can also be employed to produce a polypeptide of the invention. Cell-free translation systems can use mRNAs transcribed from a DNA construct comprising a promoter operably linked to a nucleic acid encoding the polypeptide or fragment thereof. In some aspects, the DNA construct may be linearized prior to conducting an in vitro transcription reaction. The transcribed mRNA is then incubated with an appropriate cell-free translation extract, such as a rabbit reticulocyte extract, to produce the desired polypeptide or fragment thereof.

An AT or oxidoreductase polypeptide, a fragment, or a variant thereof can be assayed for activity by any number of methods. Methods of detecting or measuring the activity of an enzymatic polypeptide generally include combining a polypeptide, fragment or variant thereof with an appropriate substrate and determining whether the amount of substrate decreases and/or the amount of product or by-product increases. The substrates used to evaluate the activity of DATs disclosed herein typically were tryptophan and/or R-MP, and the products were indole-3-pyruvate and/or R,R-monatin. In addition to a tryptophan or MP substrate, it is expected that polypeptides disclosed herein also will utilize substituted tryptophan and/or MP substrates such as, without limitation, chlorinated tryptophan or 5-hydroxytryptophan. In addition, a by-product of the conversion of MP to monatin (e.g., 4-hydroxy-4-methyl glutamic acid (HMG)) can be monitored or measured.

Methods for evaluating AT activity are described, for example, in Sugio et al., 1995, Biochemistry, 34:9661-9669; Ro et al., 1996, FEBS Lett., 398:141-145; or Gutierrez et al., 2000, Eur. J. Biochem., 267, 7218-7223. In addition, methods for evaluating dehydrogenase activity are described, for example, in Lee et al., 2006, AEM, 72(2):1588-1594; and Mayer, 2002, J. Biomolecular Screening, 7(2):135-140. In addition, methods of evaluating candidate polypeptides for DAT activity are described in Part A and Part B of the Example section herein. For the purposes of determining whether or not a polypeptide falls within the scope of the invention, the methods described in Part B of the Example section are employed.

Typically, an AT or oxidoreductase polypeptide exhibits activity in the range of between about 0.05 to 20 units (e.g., about 0.05, 0.10, 0.20, 0.30, 0.40, 0.50, 0.60, 0.70, 0.80, 0.90, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 19.5 or more units). As used herein, a unit equals one μmol of product released per minute per mg of enzyme. In one embodiment, one unit of activity for an AT polypeptide is one μmol of alpha-keto acid or ketone produced per minute per mg of enzyme (formed from the respective alpha-amino acid or amine). In an alternative embodiment, one unit of activity for an aminotransferase polypeptide is one μmol of alpha-amino acid or amine produced per minute per mg of enzyme (formed from the respective alpha-keto acid or ketone).

The conversion of tryptophan to indole-3-pyruvate or the conversion of MP to monatin using one or more of the AT or oxidoreductase nucleic acids or polypeptides disclosed herein can be performed in vitro or in vivo, in solution or in a host cell, in series or in parallel. When one or more reactions are performed in vitro, the desired ingredients for the reaction(s) can be combined by admixture in an aqueous reaction medium or solution and maintained for a period of time sufficient for the desired product(s) to be produced. Alternatively, one or more AT or oxidoreductase polypeptides used in the one or more of the reactions described herein can be immobilized onto a solid support. Examples of solid supports include those that contain epoxy, aldehyde, chelators, or primary amine groups. Specific examples of suitable solid supports include, but are not limited to, Eupergit® C (Rohm and Haas Company, Philadelphia, Pa.) resin beads and SEPABEADS® EC-EP (Resindion).

To generate indole-3-pyruvate from typtophan or monatin from MP in vivo, an AT or oxidoreductase nucleic acid (e.g., an expression vector) can be introduced into any of the host cells described herein. Depending upon the host cell, many or all of the co-factors (e.g., a metal ion, a co-enzyme, a pyridoxal-phosphate, or a phosphopanthetheine) and/or substrates necessary for the conversion reactions to take place can be provided in the culture medium. After allowing the in vitro or in vivo reaction to proceed, the efficiency of the conversion can be evaluated by determining whether the amount of substrate has decreased or the amount of product has increased.

In some embodiments, the activity of one or more of the AT or oxidoreductase polypeptides disclosed herein can be improved or optimized using any number of strategies known to those of skill in the art. For example, the in vivo or in vitro conditions under which one or more reactions are performed such as pH or temperature can be adjusted to improve or optimize the activity of a polypeptide. In addition, the activity of a polypeptide can be improved or optimized by re-cloning the AT or oxidoreductase nucleic acid into a different vector or construct and/or by using a different host cell. For example, a host cell can be used that has been genetically engineered or selected to exhibit increased uptake or production of tryptophan (see, for example, U.S. Pat. No. 5,728,555). Further, the activity of an AT or oxidoreductase polypeptide can be improved or optimized by ensuring or assisting in the proper folding of the polypeptide (e.g., by using chaperone polypeptides) or in the proper post-translational modifications such as, but not limited to, acetylation, acylation, ADP-ribosylation, amidation, glycosylation, hydroxylation, iodination, methylation, myristolyation, oxidation, pegylation, phosphorylation, prenylation, selenoylation, sulfation, disulfide bond formation, and demethylation as well as covalent attachment of molecules such as flavin, a heme moiety, a nucleotide or nucleotide derivative, a lipid or lipid derivative, and/or a phosphytidylinositol. In addition, the solubility of a polypeptide can be increased using any number of methods known in the art such as, but not limited to, low temperature expression or periplasmic expression.

A number of polypeptides were identified herein that exhibit DAT activity using tryptophan and/or MP as a substrate. Specifically, SEQ ID NO:950, 946, 948, 892, 894, 866, 870, 870 T242N, 872, 874, 878, 880, 882, 884, 902, 910, 918, 176, 178, 154, 220, 156, 216, 238, 224, 230, 232, 214, CbDAT, CaDAT and LsDAT exhibit DAT activity. Notably, SEQ ID NO:946, 892, 894, 220, 176, 1064, 1066 and 1068 exhibited very high activity under the conditions described in Part B of the Examples. It is noted that SEQ ID NO:220 and 894 produced low levels of the HMG by-product during the conversion of MP to monatin.

Use of Aminotransferase or Oxidoreductase Nucleic Acids or Polypeptides in the Production of Monatin

One or more of the DAT polypeptides disclosed herein can be used in the production of monatin. Monatin is a high-intensity sweetener having the chemical formula:

Monatin includes two chiral centers leading to four potential stereoisomeric configurations. The R,R configuration (the “R,R stereoisomer” or “R,R monatin”); the S,S configuration (the “S,S stereoisomer” or “S,S monatin”); the R,S configuration (the “R,S stereoisomer” or “R,S monatin”); and the S,R configuration (the “S,R stereoisomer” or “S,R monatin”). As used herein, unless stated otherwise, the term “monatin” is used to refer to compositions including all four stereoisomers of monatin, compositions including any combination of monatin stereoisomers, (e.g., a composition including only the R,R and S,S, stereoisomers of monatin), as well as a single isomeric form (or any of the salts thereof). Due to various numbering systems for monatin, monatin is known by a number of alternative chemical names, including: 2-hydroxy-2-(indol-3-ylmethyl)-4-aminoglutaric acid; 4-amino-2-hydroxy-2-(1H-indol-3-ylmethyl)-pentanedioic acid; 4-hydroxy-4-(3-indolylmethyl)glutamic acid; and, 3-(1-amino-1,3-dicarboxy-3-hydroxy-but-4-yl)indole.

Methods of producing various stereoisomers of monatin (e.g., R,R monatin) are disclosed in, for example, WO 07/133,183 and WO 07/103,389. One or more of the DAT polypeptides disclosed herein, in the presence of tryptophan, can be used in methods known to those of skill in the art to make a monatin composition. As disclosed in both WO 07/133,183 and WO 07/103,389, the conversion of indole-3-pyruvate (or derivatives thereof; see, for example, WO 07/103,389) to 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid (“monatin precursor” or “MP”) dictates the first chiral center of monatin, while the conversion of MP to monatin dictates the second chiral center. In on embodiment, one or more of the conversions required to produce monatin is catalyzed by more than one enzyme, for example, a mixture of enzymes, so that the resulting composition or preparation contains a desired percentage (e.g., minimum and/or maximum) of one or more of the monatin stereoisomers (e.g., R,R monatin). Alternatively, monatin made by two separate engineered pathways according to the methods of the invention be combined to produce a composition or preparation containing such desired percentage of each monatin stereoisomer(s).

Monatin that is produced utilizing one or more of the AT polypeptides disclosed herein can be at least about 0.5-30% R,R-monatin by weight of the total monatin produced. In other embodiments, the monatin produced using one or more of the polypeptides or biosynthetic pathways disclosed herein, is greater than 30% R,R-monatin, by weight of the total monatin produced; for example, the R,R-monatin is 40%, 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 99% of the total monatin produced. Alternatively, various amounts of two or more preparations of monatin can be combined so as to result in a preparation that is a desired percentage of R,R-monatin. For example, a monatin preparation that is 30% R,R-monatin can be combined with a monatin preparation that is 90% R,R-monatin; if equal amounts of 30% and 90% R,R-monatin preparations are combined, the resulting monatin preparation would be 60% R,R-monatin.

Monatin produced using one or more of the DAT polypeptides disclosed herein can be for example, a derivative. “Monatin derivatives” have the following structure:

wherein, R_(a), R_(b), R_(c), R_(d), and R_(e) each independently represent any substituent selected from a hydrogen atom, a hydroxyl group, a C₁-C₃ alkyl group, a C₁-C₃ alkoxy group, an amino group, or a halogen atom, such as an iodine atom, bromine atom, chlorine atom, or fluorine atom. However, R_(a), R_(b), R_(c), R_(d), and R_(e) cannot simultaneously all be hydrogen. Alternatively, R_(b) and R_(c), and/or R_(d) and R_(e) may together form a C₁-C₄ alkylene group, respectively. “Substituted monatin” refers to, for example, halogenated or chlorinated monatin or monatin derivatives. See, for example, U.S. Publication No. 2005/0118317.

Monatin derivatives also can be used as sweeteners. For example, chlorinated D-tryptophan, particularly 6-chloro-D-tryptophan, which has structural similarities to R,R monatin, has been identified as a non-nutritive sweetener. Similarly, halogenated and hydroxy-substituted forms of monatin have been found to be sweet. See, for example, U.S. Publication No. 2005/0118317. Substituted indoles have been shown in the literature to be suitable substrates for PLP-enzymes and have yielded substituted tryptophans. See, for example, Fukuda et al., 1971, Appl. Environ. Microbiol., 21:841-43. The halogen does not appear to sterically hinder the catalytic mechanism or the enantiospecificity of the enzyme. Therefore, halogens and hydroxyl groups should be substitutable for hydrogen, particularly on positions 1-4 of the benzene ring in the indole of tryptophan, without interfering in subsequent conversions to D- or L-tryptophan, indole-3-pyruvate, MP, or monatin.

One or more of the DAT polypeptides disclosed herein, with or without one or more additional polypeptides, can be used in the production of monatin. A DAT polypeptide (or nucleic acid molecule encoding such a DAT polypeptide) can be used in the conversion of tryptophan to indole-3-pyruvic acid (in the presence of an amino acceptor) and in the conversion of MP to monatin. The intermediate step between those two reactions is the conversion of indole-3-pyruvic acid to MP, which requires the presence of a C3 carbon source such as pyruvate, oxaloacetate or serine. The use of a DAT polypeptide in the conversion step from MP to monatin results in the R configuration at the second chiral center. It is noted that SEQ ID NO:946, 950, 220 and 948 produced high amounts of R,R monatin.

The conversion of indole-3-pyruvate (or indole-3-pyruvic acid) to MP can occur in the absence of an enzyme (i.e., an aldol condensation), but also can be facilitated by a polypeptide. The chirality at the first chiral center is determined by the enantiospecificity of the reaction converting indole-3-pyruvate to MP. If the MP formation reaction is not facilitated by an enzyme, a racemic mixture of R-MP and S-MP is typically formed in the absence of a chiral auxiliary. Enzymes that facilitate the conversion of indole-3-pyruvate to MP include, for example, a synthase/lyase (EC 4.1.3.- and 4.1.2.-), specifically those in classes EC 4.1.3.16 and EC 4.1.3.17. These classes include carbon-carbon synthases/lyases, such as aldolases that catalyze the condensation of two carboxylic acid substrates. Enzyme class EC 4.1.3.- are those synthases/lyases that form carbon-carbon bonds utilizing oxo-acid substrates (such as indole-3-pyruvate) as the electrophile, while EC 4.1.2.- are synthases/lyases that form carbon-carbon bonds utilizing aldehyde substrates (such as benzaldehyde) as the electrophile. For example, KHG aldolase (EC 4.1.3.16) and HMG aldolase (EC 4.1.3.17), are known to convert indole-3-pyruvate and pyruvate to MP. Herein, the term HMG aldolase is used to mean any polypeptide with 4-hydroxy-4-methyl-2-oxoglutarate aldolase activity. Suitable examples of HMG aldolases include Comamonas testosteroni ProA and Sinorhizobium meliloti ProA (NCBI Accession No. CAC46344).

When one or more of the conversion reactions in the pathway to producing monatin are to be performed in vivo, a person of ordinary skill in the art may optimize production of monatin in a microorganism, including R,R monatin, by various routine methods. Such a microorganism can be one that naturally is better than other microorganisms in one or more of the following characteristics, or that has been modified to exhibit one or more of the following characterisitics, which typically result in improved production of monatin (relative to the microorganism before such modification). Such characteristics include, without limitation, an increase in the ability of a microorganism to take-up tryptophan (e.g., D-tryptophan); an increase in the ability of a microorganism to take-up indole-3-pyruvate; a decrease in the ability of a microorganism to secrete indole-3-pyruvate; a decrease in the amount of degradation of indole-3-pyruvate in the microorganism; and/or a decrease in the toxicity of D-tryptophan to a microorganism. Such characteristics and strategies for obtaining microorganisms exhibiting one or more such characteristics are described in, for example, WO 07/133,183 and WO 07/103,389.

Monatin, or an intermediate of the tryptophan to monatin biosynthetic pathway (including indole-3-pyruvate and MP) produced using one or more of the AT or oxidoreductase polypeptides disclosed herein can be purified from the components of the reaction. In one embodiment, the monatin or an intermediate can be purified simply by removing the substance that is to be purified from the enzyme preparation in which it was synthesized. In other embodiments, monatin or an intermediate is purified from a preparation in which it was synthesized so that the resulting “purified” composition or preparation is at least about 5-60% monatin by weight of total organic compounds. In another embodiment, the monatin or an intermediate can be purified to a degree of purity of at least about 70%, 80%, 90%, 95% or 99% by weight of total organic compounds. The monatin produced using one or more of the polypeptides or biosynthetic pathways disclosed herein can be purified from the components of the reaction by any method known to a person of ordinary skill in the art (e.g., repeatedly recrystallization).

In accordance with the present invention, there may be employed conventional molecular biology, microbiology, biochemical, and chemical techniques within the skill of the art. Such techniques are explained fully in the literature. The invention will be further described in the following examples, which do not limit the scope of the invention described in the claims.

EXAMPLES

The aminotransferases and oxidoreductases described herein were obtained using a selection strategy. In this selection strategy, environmental DNA libraries were constructed in a bacterial host strain that exhibits L-tryptophan auxotrophy. Library clones were innoculated onto media containing D-tryptophan (but lacking L-tryptophan). The only clones that could grow are those that expressed a gene on one of the discrete environmental DNA fragments that encoded an enzyme active on D-tryptophan. For example, clones were identified that expressed an active tryptophan racemase and were able to convert D-tryptophan to L-tryptophan. Additionally, clones were identified that expressed an oxidoreductase (such as an amino acid oxidase or a dehydrogenase) that could convert D-tryptophan to an intermediate that the host cell could, in turn, convert to L-tryptophan. In the case of oxidoreductases such as amino acid oxidases and dehydrogenases, one such intermediate is indole-3-pyruvate.

The Examples in Part A describe the methodologies used for initial characterization of the candidate DAT and oxidoreductase nucleic acids and the encoded polypeptides. Further characterization of particular nucleic acids and polypeptides is described in Part B.

Part A Example 1 Growth and Assay Procedures #1

Enzyme Preparation

Glycerol stocks were used to inoculate flasks containing 50 mL of LB medium with the appropriate antibiotic. The starter cultures were grown overnight at 37° C. with shaking at 230 rpm and the OD_(600nm) was checked. The starter culture was used to inoculate 400 mL to OD_(600nm) of 0.05. The culture was incubated at 37° C. with shaking at 230 rpm.

Cultures were induced with 1 mM IPTG when the OD_(600nm), was between 0.5 and 0.8 and incubated at 30° C. and 230 rpm overnight. Cultures were harvested by pelleting cells by centrifugation at 4000 rpm for 15 minutes. The supernatant was poured off and the pellet was either frozen for later use or resuspended in 20 mL of 50 mM sodium phosphate buffer (pH 7.5) supplemented with 26 U/mL of DNAse and lysed using a microfluidizer (Microfluidics Corporation, Newton, Mass.) per the manufacturer's instructions. The clarified lysate was collected and centrifuged at 11,000 rpm for 30 minutes. The supernatant was collected in a clean tube and filtered through a 0.2 μm filter. Five mL aliquots of the clarified lysate were placed in a vial and freeze-dried using a lyophilizer (Virtis Company, Gardinier, N.Y.) per the manufacturer's instructions. Approximately 1 mL of the clarified lysate was retained for protein quantitation using the Bio-Rad Protein Assay Reagent (Bio-Rad, Hercules, Calif.) and SDS-PAGE analysis. Then, the amount of protein in each lyophilized sample was calculated.

Activity Assay

Enzymes for activity assays were prepared in 50 mM sodium phosphate pH 7.5. DAT assays were usually performed using approximately 1 mg/mL total protein.

DAT Assay Using RR-Monatin Substrate

Twenty-five mM RR-monatin, 25 mM pyruvic acid sodium salt, 0.08 mM PLP, 90 mM sodium phosphate pH 8.0 and 0.8 mg/mL DAT (total protein) prepared as described above (under ‘Enzyme Preparation’ section) were combined and incubated at 30° C. at 300 rpm. At various timepoints (generally 0, 2, 4, and 24 hours), 50 μL of the reaction product was transferred to 150 μL of ice cold acetonitrile, and the sample vortexed for 30 seconds. Samples were centrifuged at 13,200 rpm for 10 minutes at 4° C., and the supernatant was passed through a 0.45 μm filter. The filtrate was diluted 10-fold in methanol, and samples were analyzed by LC/MS/MS to monitor the D-alanine formed (described in this Example below under ‘LC/MS/MS method for detecting D-alanine or R,R-monatin’ section).

DAT Assay Using Tryptophan Substrate

Ten mM D-tryptophan, 25 mM pyruvic acid sodium salt, 0.08 mM PLP, 90 mM sodium phosphate pH 8.0, and 0.8 mg/mL DAT (total protein) prepared as described above (under ‘Enzyme Preparation’ section) were combined and incubated at 30° C. and 300 rpm. At timepoints (generally 0, 2, 4, and 24 hours), 50 μL of the reaction product was transferred to 150 μL of ice cold acetonitrile, vortexed for 30 seconds, and centrifuged at 13,200 rpm for 10 minutes at 4° C. The supernatant was passed through a 0.45 μm filter and the filtrate was diluted 10-fold in methanol. Samples were analyzed by LC/MS/MS to monitor the D-alanine formed (described in this Example below under ‘LC/MS/MS method for detecting D-alanine or R,R-monatin’ section).

LC/MS/MS Method for Detecting D-Alanine or R,R-Monatin

LC/MS/MS screening was achieved by injecting samples from 96-well plates using a CTCPal auto-sampler (LEAP Technologies, Carrboro, N.C.) into a 30/70H₂O/Acn (0.1% formic acid) mixture provided by LC-10ADvp pumps (Shimadzu, Kyoto, Japan) at 1.0 mL/min through a Zorbax Eclipse XDB-C8 (2.1×50 mm) column and into the API4000 TurboIon-Spray triple-quad mass spectrometer (Applied Biosystems, Foster City, Calif.).

Ion spray and Multiple Reaction Monitoring (MRM) were performed for the analytes of interest in the positive ion mode. alanine: parent/daughter ions: 90.12/44.25 monatin: parent/daughter ions: 293.11/130.15.

Example 2 Activity of DATs Using Assay Procedures #1

The vector pSE420-cHis is a derivative of pSE420 (Invitrogen, Carlsbad, Calif.). For pSE420-cHis, the vector was cut with NcoI and Hind III, and ligated with C-His. C-His: 5′-CCA TGG GAG GAT CCA GAT CTC ATC ACC ATC ACC ATC ACT AAG CTT (SEQ ID NO:977). The expression of the His-tag in this vector depends on the choice of host and stop codon. That is, if a TAG stop codon and a supE host are used, the His-tag is expressed; if a TAG stop codon and a non supE host are used, the His-tag is not expressed. Unless indicated otherwise, the His-tag was not expressed in these experiments.

The DAT subclones were in the pSE420-cHis vector/E. coli HMS174 host (Novagen, San Diego, Calif.) with the exception of the following subclones: SEQ ID NO:930, 932, 936 were in the pET101 D-Topo vector/BL21Star(DE3) host (Invitrogen, Carlsbad, Calif.); SEQ ID NO:934 was in the pET101 D-Topo vector/BL21 Codon PlusRIL host (Stratagene, La Jolla, Calif.); SEQ ID NO:938, 942, 944, 946 were in the pSE420 vector/XL1Blue host (Stratagene, La Jolla, Calif.); SEQ ID NO:940, 948, 950, 962 and 966 were in the pSE420-c-His vector/XL1Blue host (Stratagene, La Jolla, Calif.); and SEQ ID NO:928 was in the pQET1 vector/M15pREP4 host (pQET1 described in U.S. Pat. Nos. 5,814,473 and 6,074,860; M15pREP4 from Qiagen; Valencia, Calif.).

The subclones were grown, lysed and lyophilized according to the procedures described in Example 1. Samples were tested for activity on R,R-monatin as well as D-tryptophan (as described in Example 1). For the monatin DAT assay, DATs were incubated with 25 mM R,R-monatin, 25 mM pyruvic acid sodium salt, and 0.08 mM PLP (pH 8) at 30° C. For the D-tryptophan DAT assay, DATs were incubated with 10 mM D-tryptophan, 25 mM pyruvic acid sodium salt, and 0.08 mM PLP (pH 8) at 30° C. All DATs were loaded at 0.8 mg/mL total protein in both assays.

At indicated timepoints, 50 μL of the reaction product was added to 150 μL of ice-cold acetonitrile. Samples were vortexed for 30 seconds and the supernatant was then diluted ten-fold in methanol. Samples were then analyzed by LC/MS/MS (as described in Example 1) to monitor the D-alanine formed. The tables below show the D-aminotransferase activity on both substrates.

TABLE 1 Activity of D-aminotransferase subclones on R,R-monatin and D-tryptophan Activity on Activity on R,R-monatin D-tryptophan μg/mL D-alanine μg/mL D-alanine formed at formed at Relative SEQ ID NO: indicated hour indicated hour Expression 928 30@24 hr NT + 938 122@24 hr  NT ++ 940  5@24 hr NT ND 942 12@24 hr NT ND 944 75@24 hr NT ND 946 39@24 hr NT ND 948 200@0.5 hr   441@0.5 hr  ND 950 75@0.5 hr  452@0.5 hr  ND 962 NT NT + 964  7@24 hr ND@24 hr ++ 966 NT NT ++ 968 6.7@24 hr   52@24 hr +++ 886 (expressed in NT NT ++ XL1Blue cells) 886 (expressed in 15.4@24 hr   143@24 hr +++ E. coli HMS174 cells) NT, not tested; ND, not detected under conditions used; +, low expression, ++, moderate expression, +++, high expression Activity on Activity on R,R-monatin D-tryptophan μg/mL D-alanine μg/mL D-alanine formed at formed at Relative Subclone name indicated hour indicated hour Expression 888 (expressed in NT NT ++ XL1 Blue cells) 888 (expressed in  7@24 hr 317@24 hr +++ HMS174 cells) 890 (expressed in NT NT ++ XL1 Blue cells) 890 (expressed in  54@24 hr 278@24 hr +++ HMS174 cells) 892 (expressed in NT NT + XL1 Blue cells) 892 (expressed in 113@24 hr  <5@24 hr ++ HMS174 cells) 894 (expressed in NT NT ND XL1 Blue cells) 894 (expressed in  16@24 hr 116@24 hr + HMS174 cells) 866  28@24 hr NT +++ 868 <1@24 hr NT +++ 970 10.8@24 hr  NT + 870 123.5@24 hr   NT +++ 872 62.3@24 hr  NT +++ 874 46.5@24 hr  NT +++ 876  44@24 hr NT ++ 878  37@24 hr NT +++ 972 <5@24 hr NT + 880 72.4@24 hr  79.6@24 hr  + 882 158.8@24 hr    344@2 hr +++ 884 290@24 hr  363@2 hr ++ 896  54@24 hr  450@2 hr +++ 898 466@24 hr 300@24 hr + 900 135@24 hr 154@24 hr + 902 280@24 hr 130@24 hr ++ 904 170@24 hr 140@24 hr + 906 700@24 hr 500@24 hr +++ 908  55@24 hr  45@24 hr + insoluble 910 384@24 hr 240@24 hr +++ 912 NT NT ND 914 NT NT ND 916 NT NT ND 918 NT NT ND 920 NT NT ND 922 NT NT ND 924 NT NT ND 926 NT NT ND 974 (expressed in NT NT ND HMS174 cells) 974 (expressed in NT NT ND XL1 Blue cells) 930 NT NT ND 932 NT NT ND 934 NT NT ND 936 NT NT ND 976 (expressed in NT  <5@24 hr ++ LX1 Blue cells) NT, not tested; ND, not detected; +, low expression; ++, moderate expression; +++, high expression

It should be noted that there are only very conservative differences between the subclones listed above and their native sequences that are also in the sequence listing. For example, quite often, a start or stop codon was modified to be more efficient for expression in E. coli. It is expected that cloning of the wildtype sequences would give similar results in terms of DAT activity. For clarification purposes, the following table shows the relationship between a number of the clones and subclones described herein.

Clone/ Sequence type subclone SEQ ID (clone or pair NO: Activity subclone) 1 31, 32 D-AT Clone 1 867, 868 D-AT Subclone 2 955, 956 D-AT Clone 2 929, 930 D-AT Subclone 3 957, 958 D-AT Clone 3 931, 932 D-AT Subclone 4 959, 960 D-AT Clone 4 935, 936 D-AT Subclone 5 41, 42 D-AT Clone 5 869, 870 D-AT Subclone 6 7, 8 D-AT Clone 6 943, 944 D-AT Subclone 7 11, 12 D-AT Clone 7 941, 942 D-AT Subclone 8 83, 84 D-AT Clone 8 879, 880 D-AT Subclone 9 151, 152 D-AT Clone 9 913, 914 D-AT Subclone 10 951, 952 D-AT Clone 10 933, 934 D-AT Subclone 11 75, 76 D-AT Clone 11 881, 882 D-AT Subclone 12 87, 88 D-AT Clone 12 883, 884 D-AT Subclone 13 163, 164 D-AT Clone 13 921, 922 D-AT Subclone 14 145, 146 D-AT Clone 14 919, 920 D-AT Subclone 15 149, 150 D-AT Clone 15 925, 926 D-AT Subclone 16 147, 148 D-AT Clone 16 915, 916 D-AT Subclone 17 15, 16 D-AT Clone 17 947, 948 D-AT Subclone 18 17, 18 D-AT Clone 18 949, 950 D-AT Subclone 19 3, 4 D-AT Clone 19 937, 938 D-AT Subclone 20 5, 6 D-AT Clone 20 939, 940 D-AT Subclone 21 161, 162 D-AT Clone 21 923, 924 D-AT Subclone 22 953, 954 D-AT Clone 22 927, 928 D-AT Subclone 23 19, 20 D-AT Clone 23 885, 886 D-AT Subclone 24 21, 22 D-AT Clone 24 891, 892 D-AT Subclone 25 23, 24 D-AT Clone 25 893, 894 D-AT Subclone 26 13, 14 D-AT Clone 26 945, 946 D-AT Subclone 27 143, 144 D-AT Clone 27 917, 918 D-AT Subclone 28 43, 44 D-AT Clone 28 871, 872 D-AT Subclone 29 45, 46 D-AT Clone 29 873, 874 D-AT Subclone 30 49, 50 D-AT Clone 30 897, 898 D-AT Subclone 31 51, 52 D-AT Clone 31 875, 876 D-AT Subclone 32 37, 38 D-AT Clone 32 877, 878 D-AT Subclone 33 25, 26 D-AT Clone 33 889, 890 D-AT Subclone 34 27, 28 D-AT Clone 34 887, 888 D-AT Subclone 35 131, 132 D-AT Clone 35 909, 910 D-AT Subclone 36 53, 54 D-AT Clone 36 865, 866 D-AT Subclone 37 29, 30 D-AT Clone 37 895, 896 D-AT Subclone 38 125, 126 D-AT Clone 38 907, 908 D-AT Subclone 39 133, 134 D-AT Clone 39 911, 912 D-AT Subclone 40 127, 128 D-AT Clone 40 899, 900 D-AT Subclone 41 137, 138 D-AT Clone 41 901, 902 D-AT Subclone 42 139, 140 D-AT Clone 42 903, 904 D-AT Subclone 43 129, 130 D-AT Clone 43 905, 906 D-AT Subclone 44 33, 34 D-AT Clone 44 969, 970 D-AT Subclone 45 219, 220 D-AT Clone 45 973, 974 D-AT Subclone 46 39, 40 D-AT Clone 46 971, 972 D-AT Subclone 47 1, 2 D-AT Clone 47 975, 976 D-AT Subclone 48 253, 254 Dehydrogenase Clone 48 961, 962 Dehydrogenase Subclone

Part B Example 3 Detection of Monatin, MP, Tryptophan, Alanine, and HMG

This example describes the analytical methodology associated with the further characterization of selected D-aminotransferase (DAT) enzymes.

LC/MS/MS Multiple Reaction Monitoring (MRM) Analysis of Monatin and Tryptophan

Analyses of mixtures for monatin and tryptophan derived from biochemical reactions were performed using a Waters/Micromass® liquid chromatography-tandem mass spectrometry (LC/MS/MS) instrument including a Waters 2795 liquid chromatograph with a Waters 996 Photo-Diode Array (PDA) absorbance monitor placed in series between the chromatograph and a Micromass® Quattro Ultima® triple quadrupole mass spectrometer. LC separations were made using an Xterra MS C8 reversed-phase chromatography column, 2.1 mm×250 mm at 40° C. The LC mobile phase consisted of A) water containing 0.3% formic acid and 10 mM ammonium formate and B) methanol containing 0.3% formic acid and 10 mM ammonium formate.

The gradient elution was linear from 5% B to 45% B, 0-8.5 min, linear from 45% B to 90% B, 8.5-9 min, isocratic from 90% B to 90% B, 9-12.5 min, linear from 90% B to 5% B, 12.5-13 min, with a 4 min re-equilibration period between runs. The flow rate was 0.27 mL/min, and PDA absorbance was monitored from 210 nm to 400 nm. All parameters of the ESI-MS were optimized and selected based on generation of protonated molecular ions ([M+H]+) of the analytes of interest, and production of characteristic fragment ions. The following instrumental parameters were used for LC/MS/MS Multiple Reaction Monitoring (MRM) analysis of monatin and tryptophan: Capillary: 3.5 kV; Cone: 40 V; Hex 1: 20 V; Aperture: 0 V; Hex 2: 0 V; Source temperature: 100° C.; Desolvation temperature: 350° C.; Desolvation gas: 500 L/h; Cone gas: 50 L/h; Low mass resolution (Q1): 12.0; High mass resolution (Q1): 12.0; Ion energy: 0.2; Entrance: −5 V; Collision Energy: 8; Exit: 1 V; Low mass resolution (Q2): 15; High mass resolution (Q2): 15; Ion energy (Q2): 3.5; Multiplier: 650. Four monatin-specific parent-to-daughter MRM transitions and one tryptophan specific parent-to-daughter transition are used to specifically detect monatin and tryptophan in in vitro and in vivo reactions. The transitions monitored are 293.08 to 157.94, 293.08 to 167.94, 293.08 to 130.01, and 293.08 to 256.77. Tryptophan is monitored with the MRM transition 205.0 to 146.0. For internal standard quantification of monatin and tryptophan, four calibration standards containing four different ratios of each analyte to d₅-tryptophan and d₅-monatin, are analyzed. These data are subjected to a linear least squares analysis to form a calibration curve for monatin and tryptophan. To each sample is added a fixed amount of d₅-tryptophan and d₅-monatin (d₅-monatin was synthesized from d₅-tryptophan according to the methods from WO 2003/091396 A2), and the response ratios (monatin/d₅-monatin; tryptophan/d₅-tryptophan) in conjunction with the calibration curves described above are used to calculate the amount of each analyte in the mixtures. Parent-to-daughter mass transitions monitored for d₅-tryptophan and d₅-monatin are 210.0 to 150.0, and 298.1 to 172.0 and 298.1 to 162.00 respectively.

Chiral LC/MS/MS (MRM) Measurement of Monatin

Determination of the stereoisomer distribution of monatin in biochemical reactions was accomplished by derivatization with 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide (FDAA), followed by reversed-phase LC/MS/MS MRM measurement.

Derivatization of Monatin with FDAA

To 50 μL of sample or standard and 10 μL of internal standard was added 100 μL of a 1% solution of FDAA in acetone. Twenty μL of 1.0 M sodium bicarbonate was added, and the mixture was incubated for 1 h at 40° C. with occasional mixing. The sample was removed and cooled, and neutralized with 20 μL of 2.0 M HCl (more HCl may be required to effect neutralization of a buffered biological mixture). After degassing was complete, samples were ready for analysis by LC/MS/MS.

LC/MS/MS Multiple Reaction Monitoring for the Determination of the Stereoisomer Distribution of Monatin

Analyses were performed using the LC/MS/MS instrumentation described in the previous sections. The LC separations capable of separating all four stereoisomers of monatin (specifically FDAA-monatin) were performed on a Phenomenex Luna® 2.0×250 mm (3 μm) C18 reversed phase chromatography column at 40° C. The LC mobile phase consisted of A) water containing 0.05% (mass/volume) ammonium acetate and B) acetonitrile. The elution was isocratic at 13% B, 0-2 min, linear from 13% B to 30% B, 2-15 min, linear from 30% B to 80% B, 15-16 min, isocratic at 80% B 16-21 min, and linear from 80% B to 13% B, 21-22 min, with a 8 min re-equilibration period between runs. The flow rate was 0.23 mL/min, and PDA absorbance was monitored from 200 nm to 400 nm. All parameters of the ESI-MS were optimized and selected based on generation of deprotonated molecular ions ([M−H]⁻) of FDAA-monatin, and production of characteristic fragment ions.

The following instrumental parameters were used for LC/MS analysis of monatin in the negative ion ESI/MS mode: Capillary: 3.0 kV; Cone: 40 V; Hex 1: 15 V; Aperture: 0.1 V; Hex 2: 0.1 V; Source temperature: 120° C.; Desolvation temperature: 350° C.; Desolvation gas: 662 L/h; Cone gas: 42 L/h; Low mass resolution (Q1): 14.0; High mass resolution (Q1): 15.0; Ion energy: 0.5; Entrance: 0 V; Collision Energy: 20; Exit: 0 V; Low mass resolution (Q2): 15; High mass resolution (Q2): 14; Ion energy (Q2): 2.0; Multiplier: 650. Three FDAA-monatin-specific parent-to-daughter transitions were used to specifically detect FDAA-monatin in in vitro and in vivo reactions. The transitions monitored for monatin were 542.97 to 267.94, 542.97 to 499.07, and 542.97 to 525.04. Monatin internal standard derivative mass transition monitored was 548.2 to 530.2. Identification of FDAA-monatin stereoisomers was based on chromatographic retention time as compared to purified monatin stereoisomers, and mass spectral data. An internal standard was used to monitor the progress of the reaction and for confirmation of retention time of the S,S stereoisomer.

Liquid Chromatography-Post Column Fluorescence Detection of Amino Acids, Including Tryptophan, Monatin, Alanine, and HMG

Procedure for Trytophan, Monatin, and Alanine

Liquid chromatography with post-column fluorescence detection for the determination of amino acids in biochemical reactions was performed on a Waters 2690 LC system or equivalent combined with a Waters 474 scanning fluorescence detector, and a Waters post-column reaction module (LC/OPA method). The LC separations were performed on an Interaction-Sodium loaded ion exchange column at 60° C. Mobile phase A was Pickering Na 328 buffer (Pickering Laboratories, Inc.; Mountain View, Calif.). Mobile phase B was Pickering Na 740 buffer. The gradient elution was from 0% B to 100% B, 0-20 min, isocratic at 100% B, 20-30 min, and linear from 100% B to 0% B, 30-31 min, with a 20 min re-equilibration period between runs. The flow rate for the mobile phase was 0.5 mL/min. The flow rate for the OPA post-column derivatization solution was 0.5 mL/min. The fluorescence detector settings were EX 338 nm and Em 425 nm. Norleucine was employed as an internal standard for the analysis. Identification of amino acids was based on chromatographic retention time data for purified standards.

Procedure for HMG

Samples from biochemical reactions were cleaned up by solid phase extraction (SPE) cartridges containing C18 as the packing material and 0.6% acetic acid as the eluent. The collected fraction from SPE was then brought up to a known volume and analyzed using HPLC post-column O-Phthaladehyde (OPA) derivatization with a florescence detector. Chromatographic separation was made possible using a Waters 2695 liquid chromatography system and two Phenomenex AquaC18 columns in series; a 2.1 mm×250 mm column with 5 μm particles, and a 2.1 mm×150 mm column with 3 μm particles. The temperature of the column was 40° C. and the column isocratic flow rate was 0.18 mL/min. The mobile phase was 0.6% acetic acid. OPA post-column derivatization and detection system consists of a Waters Reagent Manager (RMA), a reaction coil chamber, a temperature control module for the reaction coil chamber, and a Waters 2847 Florescent detector. The OPA flow rate was set at 0.16 mL/min, and the reaction coil chamber was set to 80° C. The florescence detector was set with an excitation wavelength of 348 nm and an emission wavelength of 450 nm. Other parameters controlling detector sensitivity, such as signal gain and attenuation, were set to experimental needs. Quantification of HMG was based off of the molar response of glutamic acid.

Detection of MP by LC/MS

Liquid chromatography separations were made using Waters 2690 liquid chromatography system and a 2.1 mm×50 mm Agilent Eclipse XDB-C18 1.8 μm reversed-phase chromatography column with flow rate at 0.25 mL/min and gradient conditions as follows:

Time (min) A % B % 0.00 95 5 0.2 95 5 1.2 5 95 4.5 5 95 5.0 95 5 10 95 5

The mobile phase A was 0.3% (v/v) formic acid with 10 mM ammonium formate, and mobile phase B was 0.3% formic acid w/10 mM ammonium formate in 50:50 methanol/acetonitrile. The column temperature was 40° C.

Parameters for the Micromass ZQ quadrupole mass spectrometer operating in negative electrospray ionization mode (−ESI) were set as follows: Capillary: 2.2 kV; Cone: 35 V; Extractor: 4 V; RF lens: 1 V; Source temperature: 120° C.; Desolvation temperature: 380° C.; Desolvation gas: 600 L/h; Cone gas: Off; Low mass resolution: 15.0; High mass resolution: 15.0; Ion energy: 0.2; Multiplier: 650. Single ion monitoring MS experiment was set up to allow detection selectively for m/z 290.3, 210.3, 184.3, and 208.4. The m/z 208.4 is the deprotonated molecular [M−H]⁻ ion of the internal standard d₅-tryptophan.

Detection of MP by LC/MS/MS

LC separations were made using Waters HPLC liquid chromatography system and a 2.1 mm×50 mm Agilent Eclipse XDB-C18 1.8 μm reversed-phase chromatography column with flow rate at 0.25 mL/min and gradient conditions are as follows:

Time (min) A % B % 0.00 95 5 0.7 95 5 3.0 5 95 4.0 5 95 4.3 95 5 6.0 95 5

Mobile phase A was 0.3% (v/v) formic acid with 10 mM ammonium formate, and B was 0.3% formic acid with 10 mM ammonium formate in 50:50 methanol/acetonitrile. The column temperature was 40° C.

Parameters on Waters Premier XE triple quadrupole mass spectrometer for LC/MS/MS Multiple Reaction Monitoring (MRM) experiments operating in negative electrospray ionization mode (−ESI) were set as the following; Capillary: 3.0 kV; Cone: 25 V; Extractor: 3 V; RF lens: 0 V; Source temperature: 120° C.; Desolvation temperature: 350° C.; Desolvation gas: 650 L/hr; Cone gas: 47 L/hr; Low mass resolution (Q1): 13.5; High mass resolution (Q1): 13.5; Ion energy (Q1): 0.5 V; Entrance: 1 V; Collision Energy: 18 V; Exit 1: 19; Low mass resolution (Q2): 15; High mass resolution (Q2): 15; Ion Energy (Q2): 2.0; Multiplier: 650. Four parent-to-daughter MRM transitions were monitored to selectively detect Monatin precursor (MP) and d₅-Monatin precursor (d₅-MP); d₅-MP was used as an internal standard (I.S.). The four MRM transitions were 290.1 to 184.1, 290.1 to 210.1, 290.1 to 228.1, and 295.1 to 189.1. Two of these transitions, 290.1 to 184.1 for MP, and 295.1 to 189.1 for d₅-MP, were used for generating calibration curves and for quantification purposes. Transitions of 290.1 to 210.1 and 290.1 to 228.1 were used as qualitative secondary confirmation of MP.

Production of Monatin and MP for Standards and for Assays

Production of Monatin

A racemic mixture of R,R and S,S monatin was synthetically produced as described in U.S. Pat. No. 5,128,482. The R,R and S,S monatin were separated by a derivatization and a hydrolysis step. Briefly, the monatin racemic mixture was esterified, the free amino group was blocked with carbamazepine (CBZ), a lactone was formed, and the S,S lactone was selectively hydrolyzed using an immobilized protease enzyme. The monatin can also be separated as described in Bassoli et al., Eur. J. Org. Chem., 8:1652-1658, (2005).

MP Production

R-MP was produced by the transamination of R,R monatin using AT-103 broad range D-aminotransferase (BioCatalytics, Pasadena, Calif.) in 0.1 M potassium phosphate buffer, using sodium pyruvate as the amino acceptor. S-MP was produced by the transamination of S,S monatin using AT-102 L-aminotransferase (BioCatalytics) in 0.1 M potassium phosphate buffer, using sodium pyruvate as the amino acceptor. Both reactions were carried out at 30° C. and at a pH of approximately 8.0-8.3, for approximately 20 hours. Both compounds were purified using preparative scale HPLC with a Rohm and Haas (Philadelphia, Pa.) hydrophobic resin (XAD™ 1600), eluting in water. Samples containing greater than 90% purity monatin precursor were collected and freeze-dried.

Example 4 Protein Preparation Methods

This example describes the methodology used for cloning, expression, cell extract preparation, protein purification, and protein quantification for secondary characterization of selected DATs.

Those of skill in the art would realize that the presence of activity in a polypeptide encoded from a subcloned (e.g., a fragment) or otherwise modified (e.g., tagged) nucleic acid is considered predictive of the presence of activity in the corresponding polypeptide encoded from the full-length or wild type nucleic acid.

Amplification of DAT-Encoding Genes for Cloning into Topo Plasmids

PCR reactions for Topo cloning (using either Pfu Turbo or Cloned Pfu from Stratagene) were as follows: 1× recommended buffer for the polymerase enzyme, 0.2 mM dNTPs, 0.5 μM of each primer, and 1 μl per 50 μl of reaction of the polymerase (2.5 units). The reactions contained approximately 5-100 ng of template DNA per reaction. A 94° C. hot start for 2 minutes was used for PCRs, as well as a melting temperature of 94° C. The annealing temperature was dependent on the Tm of the primers, and was either 30 or 60 seconds. The extension time (at 72° C.) was at least 2 min per kb. The reaction products were normally separated on a 1×TAE 1% agarose gel, and bands of appropriate sizes were purified with QIAquick Gel Extraction Kit as recommend by the manufacturer except an elution volume of 10 to 50 μl was used. Volumes of 1 to 4 μl of the purified PCR product were used for ligation with the pCRII-Topo Blunt plasmid (Invitrogen, Carlsbad, Calif.) as recommended by the manufacturer.

Cloning of DATs in pET30a for Untagged Expression

The DATs having the sequence shown in SEQ ID NO:945, 947, 949, 891, 893, 869, 873, 877, 881, 883, and 895 (encoding the polypeptides having the sequence of SEQ ID NO:946, 948, 950, 892, 894, 870, 874, 878, 882, 884, and 896) were amplified from plasmids or PCR products with Pfu Turbo (Stratagene, La Jolla, Calif.) and primers adding a Nde I at the 5′ end and either a Not I or BamH I restriction site at the 3′ end. The PCR fragments were cloned into pCR-Blunt II-Topo (Invitrogen, Carlsbad, Calif.) as recommended by the manufacturer. The sequence was verified by sequencing (Agencourt, Beverly, Mass.) and inserts with the correct sequences were then released from the vector using the appropriate restriction enzymes and ligated into the Nde I and Not I (or BamH I) restriction sites of pET30a. See Table 2 for specific primers.

The DAT nucleic acid having the sequence of SEQ ID NO:155 (encoding the polypeptide having SEQ ID NO:156) was amplified with Pfu Turbo (Stratagene) and primers adding a Nde I and Hind III restriction site at the 5′ and 3′ end, respectively. The PCR fragments were digested using Nde I and Hind III restriction enzymes and ligated into the Nde I and Hind III restriction sites of pET30a. See Table 2 for specific primers. It should be noted that the polypeptide having the sequence of SEQ ID NO:156 appeared to contain the following leader sequence with a probability of 0.991 (as determined by SignalP, as discussed in Nielsen, 1997, Protein Engineering, 10:1-6): KNSPIIAAYRAATPGSAAA (SEQ ID NO:1084). The nucleic acid encoding this DAT polypeptide was cloned with the apparent leader sequence.

TABLE 2 Primers for amplification Amplifies SEQ SEQ ID ID NO PCR primers NO: 945 5′-CCGCCCCATATGAACGCACTAGGATATTACAACGGAAAATGG-3′ 978 5′-GGCGGATCCTTATCCAAAGAATTCGGCACGAGCTGTC-3′ 979 947 5′-CCGCCCCATATGCGCGAAATTGTTTTTTTGAATGGG-3′ 980 5′-CGGATCCCTAAACCATCTCAAAAAACTTTTGCTGAATAAACCGTG-3′ 981 949 5′-CCGCCCCATATGTTGGATGAACGGATGGTGTTCATTAAC-3′ 982 5′-GGCGGATCCCTAGTCCACGGCATAGAGCCACTCGG-3′ 983 891 5′-GGCCGCATATGGACGCACTGGGATATTACAACGGAAAATG-3′ 984 5′-GGCCGCGGCCGCCTATGCCTTTCTCCACTCAGGCGTGTAGC-3′ 985 893 5′-GGCCGCATATGGACGCACTGGGATATTACAACGGAAAATG-3′ 986 5′-GGCCGCGGCCGCCTATACTGTGCTCCACTCAGGCGTGTAGCC-3′ 987 869 5′-CATATGTATTCATTATGGAATGATCAAATAGTGAAGG-3′ 988 5′-GCGGCCGCCTATTTATTCGTAAAAGGTGTTGGAATTTTCG-3′ 989 873 5′-CATATGAGCACCCCGCCGACCAATC-3′ 990 5′-GCGGCCGCCTAGGCCGCCTTCACTTCACGCTC-3′ 991 877 5′-CATATGAGCACCCCGCCAACCAATTC-3′ 992 5′-GCGGCCGCCTACGCGGCCTTCACTTCGCGC-3′ 993 881 5′-TCCAGGCATATGAGCACAGTATATTTAAATGGCC-3′ 994 5′-CCAGTAGCGGCCGCCTAACACTCAACACTATACTTATGC-3′ 995 883 5′-TCTAGGCATATGGTTTATCTGAACGGGCG-3′ 996 5′-ACTGTAGCGGCGGCCTATCCGAGGGACGCGTTGG-3′ 997 895 5′-CATATGAAAGAGCTGGGCTATTACAACGGAAAAATC-3′ 998 5′-GCGGCCGCCTATGACCTCCACCCCTGATTTCCAAAATAC-3′ 999 155 5′-CTAGGATTCCATATGAAGAATTCGCCGATCATC-3′ 1000 5′-CGAAGCTTCAACAGCGGCCGCTTAAAG-3′ 1001 Site-Directed Mutagenesis

Site-directed mutagenesis was performed using QuikChange Multi Site-Directed Mutagenesis Kit (Stratagene, La Jolla, Calif.) according to the manufacturer's instructions. To generate the SEQ ID NO:870 T242N mutant, the pET30a untagged construct described in Example 10 was used as the template. To generate SEQ ID NO:220 G240N and SEQ ID NO:220 T241N mutants, the pET30a construct with a C-terminal his tag described in Example 10 was used as the template. The mutagenic primers used are listed below in Table 3. All the desired mutations were confirmed by DNA sequencing.

TABLE 3 Primer sequences Mutant polypeptide designation SEQ ID (SEQ ID NO) Sequence NO: 870 T242N 5′-AATTATTTGTTTCATCAACAAATTCTGAAATTACGCCGGTTATTG-3′ 1002 220 G240N 5′-CTTGTGTCCAGCAGCAACACACTCGGCCTTAG-3′ 1003 220 T241N 5′-GTCCAGCAGCGGCAACCTCGGCCTTAGCGCC-3′ 1004 Cloning of DAT PCR Products in pET30a for the Expression as Untagged Protein

DAT nucleic acids having the sequences shown in SEQ ID NO:177, 179, 153, 165, 181, 217, 187, 189, 207, 219, 215, 195, 199, 197, 209, 201, 221, 235, 203, 237, 239, 223, 225, 227, 229, 231, 245, 213, 155, 169, 171, 167, 173, and 175 (encoding DAT polypeptides having the sequence shown in SEQ ID NO:178, 180, 154, 166, 182, 218, 188, 190, 208, 220, 216, 196, 200, 198, 210, 202, 222, 236, 204, 238, 240, 224, 226, 228, 230, 232, 246, 214, 156, 170, 172, 168, 174, and 176) were received as PCR products with Nde I and Not I compatible ends, as well as extraneous nucleotides to improve cutting efficiencies.

The DAT PCR products contained an NdeI restriction enzyme site at the 5′ end and a NotI site at the 3′ end. The PCR fragments were first cloned into pCR4 TOPO or pCR-Blunt II-TOPO vector (Invitrogen). After the DNA sequences were verified by sequencing, the DAT genes were released from the TOPO plasmids by the digestion of NdeI and NotI and ligated into the pET30a vector which had been cut using the same restriction enzymes. DAT genes containing either an NdeI or NotI site internally were amplified using primers with compatible restriction enzyme sites and cloned into pET30a. For example, the DAT nucleic acid having the SEQ ID NO:155 (encoding the polypeptide having the sequence of SEQ ID NO:156) was reamplified from the original PCR product using NdeI and HindIII restriction sites for cloning into pET30a.

Cloning of DATs in pET30a for the Expression as the C-His-Tagged Fusion Protein

Nucleic acids encoding DAT 4978 and DAT 4978 T243N (described in Example 6), SEQ ID NO:870 (expressed from plasmid pSE420-cHis), and SEQ ID NO:870 T242N, SEQ ID NO:176 and SEQ ID NO:220 (untagged versions expressed from pET30) were re-amplified with Pfu Turbo (Stratagene) and primers that placed an XhoI site immediately upstream of the stop codons. PCR fragments were cloned into pCR-Blunt II-Topo (Invitrogen, Carlsbad, Calif.) as recommended by the manufacturer or directly cloned into the Nde I and Xho I restriction sites of pET30a The sequence was verified by sequencing (Agencourt, Beverly, Mass.) and an insert with the correct sequence was then released from the vector using Nde I and Xho I restriction enzymes and the insert was ligated into the Nde I and Xho I restriction sites of pET30a. See Table 4 for specific primers and plasmids names.

TABLE 4 Primer sequences Polypeptide SEQ designation ID (SEQ ID NO) Sequence NO 870 and 5′-CATATGTATTCATTATGGAATGATCAAATAGTGAAGG-3′ 1005 870 T242N 5′-CTCGAGTTTATTCGTAAAAGGTGTTGGAATTTTCGTTTC-3′ 1006 DAT4978 and 5′-CATATGAGTTATAGCTTATGGAATGACCAAATTGTGAATG-3′ 1007 DAT4978T243N 5′-CTCGAGTGCGCGAATACCTTTTGGGATTTTCGTATC-3′ 1008 220 5′-CTAGGATCTCATATGGACGCACTGGGATATTAC-3′ 1009 5′-GCCTCGAGTACCCTGCTCCACTCAGG-3′ 1010 176 5′-CTAGGATTCCATATGGACGCGCTTGGCTATTAC-3′ 1011 5′-GCCTCGAGTACCCTGCTCCACGCAG-3′ 1012 Cloning of CbDAT and CaDAT

A Clostridium beijerinckii D-amino-transferase was PCR amplified using Pfu Turbo (Stratagene) and C. beijerinckii genomic DNA with PCR primers containing a 5′ NdeI and a 3′ NotI restriction site. Genomic DNA was extracted from C. beijerinckii (ATCC 51743) using the Purrgene genomic DNA purification kit (Gentra Systems, Minneapolis, Minn.) per the manufacturer's instructions.

The 824 bp PCR product was gel extracted using a Qiagen Gel Extraction Kit and TOPO cloned into pCR-Blunt II-Topo (Invitrogen). After verifying the sequence, the gene was ligated to Nde I/Not I cut pET28b and pET30a vectors using a Rapid Ligation kit (Roche).

The C. acetobutylicum DAT was amplified by PCR using genomic DNA (ATCC 824) and the Stratagene Optiprime PCR Kit with PCR primers containing a 5′ NdeI and a 3′ NotI restriction site. The successful PCR reaction was cloned into the pCR4 TOPO vector and TOPO clones were sequenced. A positive TOPO clone was digested with restriction enzymes NdeI and NotI and the DAT fragment ligated into pET30a vector digested with the same restriction enzymes.

TABLE 5 Primer sequences Designation Sequence SEQ ID NO: CbDAT1 5′-GGTTCATATGGAGAATTTAGGTTATTA-3′ 1013 5′-GGAAGCGGCCGCATATTCTACCTCCTATTCTG-3′ 1014 CaDAT2 5′-GGTTCATATGAAAGATTTAGGATATTACAATGGAGAATAC-3′ 1015 5′GGAAGCGGCCGCTTAATTTGTTTCTTCCAAAAATTCATTAAG-3′ 1016 In Vitro Synthesis of LsDAT

The Lactobacillus salivarius DAT was assembled using a revised method based on Stemmer et al., 1995, Gene, 164:49-53. Briefly, 43 oligonucleotides (primarily 40 mers) were ordered from IDT based on the gene sequence and its complementary DNA sequence, with 20 basepair overlaps between the sense and antisense strands. See Table 6 for the primer list. The primers were diluted to 250 μM in water and 5 μL of each primer was mixed together in a microfuge tube. PCR was carried out as follows: per 100 μL reaction, 1.5 μL of the primer pool, 4 μL dNTPs, 1×XL PCR buffer, 1 mM magnesium acetate, 2 μL rTth polymerase (Roche, Indianapolis, Ind.), and 0.25 μL Pfu polymerase (Stratagene, La Jolla, Calif.) were added. A 3 minute hot start was done at 94° C., followed by 15 cycles of 94° C. for 30 seconds, 42° C. for 30 seconds, and 68° C. for 15 seconds. Ten more cycles were done with an extension time of 30 seconds (at 68° C.). Ten more cycles were performed with an extension time of 75 seconds. Lastly, a chain extension step was done for seven minutes at 68° C.

TABLE 6 Oligos used to synthesis LsDAT Designation Sequence (5′ → 3′) SEQ ID NO: F1: ATGAAGCAAG TTGGATACTA CAATGGTACT ATCGCTGATT 1017 F2: TAAATGAACT TAAGGTGCCT GCTACTGATC GTGCACTTTA 1018 F3: TTTTGGTGAT GGTTGCTACG ATGCAACTAC ATTTAAGAAC 1019 F4: AATGTTGCAT TTGCCTTAGA AGATCATCTT GATCGTTTTT 1020 F5: ATAATAGTTG TCGCCTACTA GAGATCGATT TCCCTTTAAA 1021 F6: TCGCGATGAA CTTAAAGAAA AGCTTTACGC TGTTATTGAT 1022 F7: GCTAACGAAG TTGATACTGG TATCCTTTAT TGGCAAACTT 1023 F8: CACGTGGTTC TGGTTTACGT AACCATATTT TCCCAGAAGA 1024 F9: TAGCCAACCT AATTTATTAA TTTTTACTGC TCCTTATGGT 1025 F10: TTAGTTCCAT TTGATACTGA ATATAAACTT ATATCTCGCG 1026 F11: AAGACACTCG CTTCTTACAT TGCAATATTA AAACTTTGAA 1027 F12: TTTACTTCCA AACGTTATTG CAAGTCAAAA GGCTAATGAA 1028 F13: AGTCATTGCC AAGAAGTGGT CTTCCATCGC GGTGACAGAG 1029 F14: TTACAGAATG TGCACACTCT AACATCTTAA TTCTAAAAGA 1030 F15: TGGCGTTCTT TGCTCCCCAC CTAGAGATAA TTTAATCTTG 1031 F16: CCAGGAATTA CTTTGAAACA TCTCTTGCAA TTAGCAAAAG 1032 F17: AAAATAATAT TCCTACTTCC GAAGCACCAT TCACTATGGA 1033 F18: TGATCTTAGA AATGCTGATG AAGTTATTGT TAGTTCTTCA 1034 F19: GCTTGTCTAG GTATTCGCGC AGTCGAGCTT GATGGTCAGC 1035 F20: CTGTTGGTGG AAAAGATGGA AAGACTTTAA AGATCTTGCA 1036 F21: AGATGCTTAT GCTAAGAAAT ATAATGCTGA AACTGTAAGT CGTTAA 1037 R1: TAGTATCCAA CTTGCTTCAT 1038 R2: AGGCACCTTA AGTTCATTTA AATCAGCGAT AGTACCATTG 1039 R3: CGTAGCAACC ATCACCAAAA TAAAGTGCAC GATCAGTAGC 1040 R4: TCTAAGGCAA ATGCAACATT GTTCTTAAAT GTAGTTGCAT 1041 R5: TAGTAGGCGA CAACTATTAT AAAAACGATC AAGATGATCT 1042 R6: TTTCTTTAAG TTCATCGCGA TTTAAAGGGA AATCGATCTC 1043 R7: CCAGTATCAA CTTCGTTAGC ATCAATAACA GCGTAAAGCT 1044 R8: ACGTAAACCA GAACCACGTG AAGTTTGCCA ATAAAGGATA 1045 R9: TTAATAAATT AGGTTGGCTA TCTTCTGGGA AAATATGGTT 1046 R10: TCAGTATCAA ATGGAACTAA ACCATAAGGA GCAGTAAAAA 1047 R11: ATGTAAGAAG CGAGTGTCTT CGCGAGATAT AAGTTTATAT 1048 R12: CAATAACGTT TGGAAGTAAA TTCAAAGTTT TAATATTGCA 1049 R13: ACCACTTCTT GGCAATGACT TTCATTAGCC TTTTGACTTG 1050 R14: AGAGTGTGCA CATTCTGTAA CTCTGTCACC GCGATGGAAG 1051 R15: GTGGGGAGCA AAGAACGCCA TCTTTTAGAA TTAAGATGTT 1052 R16: TGTTTCAAAG TAATTCCTGG CAAGATTAAA TTATCTCTAG 1053 R17: GGAAGTAGGA ATATTATTTT CTTTTGCTAA TTGCAAGAGA 1054 R18: CATCAGCATT TCTAAGATCA TCCATAGTGA ATGGTGCTTC 1055 R19: GCGCGAATAC CTAGACAAGC TGAAGAACTA ACAATAACTT 1056 R20: TCCATCTTTT CCACCAACAG GCTGACCATC AAGCTCGACT 1057 R21: ATTTCTTAGC ATAAGCATCT TGCAAGATCT TTAAAGTCTT 1058 R22: TTAACGACTT ACAGTTTCAG CATTAT 1059

A secondary amplification with primers L. sal DAT R Not I and L. sal DAT F Nde I (below) resulted in a band of the correct molecular weight. See Table 7 for these secondary amplification primer sequences.

TABLE 7 Primer sequences Designation Sequence (5′ → 3′) SEQ ID NO: L. sal DAT R NotI TTGGCCAAGCGGCCGCTTAACGACTTACAGTTT 1060 L. sal DAT F NdeI GGTTCCAAGGCATATGAAGCAAGTTGGATACTA 1061

The secondary PCR reaction was set up the same as above with the exception that only 2 primers were added. For the PCR template, 2.5 μl of the primary PCR reaction was used. A 3 minute hot start was done at 94° C., followed by 10 cycles of 94° C. for 30 seconds, 42° C. for 30 seconds, and 68° C. for 15 seconds. Ten more cycles were done with an increased annealing temp of 48° C. for 30 seconds with an extension time of 30 seconds (at 68° C.). Lastly, a chain extension step was done for seven minutes at 68° C.

The fragment was cloned into a pCR-BluntII-TOPO vector and the TOPO clones were sequenced. A positive TOPO clone was cut with NdeI and NotI and the DAT fragment ligated into pET30a vector digested with the same restriction enzymes.

Enzyme Preparation

E. coli strain BL21(DE3) was used as the host strain for the expression of DATs from pET-derived plasmids. E. coli strain TOP10 was used in all other DAT constructs. Single colonies of desired constructs were typically innoculated into Overnight Express II medium (Novagen) containing the appropriate amount of antibiotics. Following cultivation at 30° C. overnight, the cells were harvested by centrifugation when the OD₆₀₀ was greater than 10. Alternatively, overnight cultures were utilized to innoculate cultures in LB medium containing the appropriate antibiotics. The cultures were grown at 30° C. to an OD₆₀₀ of 0.5 to 0.9 and protein expression was induced with 1 mM IPTG for 4 h at the same temperature.

Cell extracts were prepared by adding 5 mL per g of cell pellet or 5 mL per 50 mL of overnight culture, of BugBuster® (primary amine-free) Extraction Reagent (EMD Biosciences/Novagen catalog #70923) with 5 μL/mL of Protease Inhibitor Cocktail II (EMD Bioscience/Calbiochem catalog #539132), 1 μl/ml of Benzonase® Nuclease (EMD Biosciences/Novagen catalog #70746), and 0.033 μl/ml of r-Lysozyme™ solution (EMD Biosciences/Novagen catalog #71110) to the cells. The cell resuspension was incubated at room temperature for 15 min with gentle shaking. Following centrifugation at 16,100 rcf for 20 min at 4° C., the supernatant was removed as the cell-free extract.

Prior to using the enzyme preparation for monatin reactions, detergents and low molecular weight compounds were removed from the cell-free extract by passage through a PD-10 column (GE Healthcare, Piscataway, N.J.) that was previously equilibrated with potassium phosphate buffer (100 mM, pH 7.8) or EPPS buffer (100 mM, pH 8.2) containing 0.05 mM of PLP. The protein was eluted using the equilibration buffer. Protein concentrations were typically determined using the BioRad Coomassie plate assay (also known as the Bradford assay) plate assay with BSA (Pierce) as the standard. Occasionally, the BCA (Pierce) microtiter plate assay was used for protein determination, where noted. To estimate the concentration of the D-aminotransferase in the cell-free extracts, 1 mg/mL samples were loaded on the Experion (Bio-Rad, Hercules, Calif.) electrophoresis system and the Experion Software (Version 2.0.132.0) was used to calculate the percentage of the soluble DAT protein in the cell-free extract. Alternatively, SDS-PAGE analysis was done and visual estimation was used to estimate percentage of expression.

The His-tagged fusion proteins were purified using either the GE Healthcare Chelating Sepharose Fast Flow resin or Novagen His-Bind columns. The purification using the Sepharose resin involved loading the cell-free extract onto a column that was previously equilibrated with potassium phosphate buffer (100 mM, pH 7.8) containing 200 mM of sodium chloride and 0.050 mM of PLP. The column was then washed successively using 3-5 column volumes of the equilibration buffer, 3-5 column volumes of the equilibration buffer containing 25 mM of imidazole and 3-5 column volumes of the equilibration buffer containing 50-100 mM of imidazole. The His-tagged protein was eluted off the column using 3-5 column volumes of the equilibration buffer containing 500 mM of imidazole. The eluate was concentrated using the Amicon (Billerica, Mass.) Centricon-70. The imidazole and sodium chloride salts in the concentrated protein solution were removed by passage through PD-10 desalting columns that were previously equilibrated using potassium phosphate buffer (100 mM, pH 7.8) (for DAT4978 and DAT4978 T243N) or EPPS buffer (100 mM, pH 8.2) (for SEQ ID NO:870 and SEQ ID NO:870 T242N) containing 50 μM of PLP. Protein concentrations were determined using Bio-Rad Protein Assay (Bio-Rad) and Albumin (Pierce) as a standard. Aliquots (0.5-1 mL) of the purified enzyme were stored at −80° C. until use. The purification of the His-tagged protein using the His-Bind columns followed the manufacture's instruction. The eluate from the column was desalted using the PD10 column as described above.

Example 5 Assay Procedures #2 for D-Aminotransferase Activity

Monatin Production Assay (Standard)

The following components were combined: 100 mM EPPS, pH 8.2; 200 mM sodium pyruvate; 100 mM of D-tryptophan; 50 μM PLP; 1 mM MgCl₂; 0.01% Tween-80; 50 μg/mL of aldolase described in Example 6 (cell-free extract was used; the aldolase concentration was estimated based on the percentage reading from Experion chip) and an appropriate amount of DAT (typically 0.1-1 mg/mL).

Except for the PLP stock solution and the protein solutions, all other reagents were made using oxygen-free deionized water and stored in the anaerobic chamber. The reactions were set up in the anaerobic chamber at room temperature with constant gentle mixing. To take a time point, formic acid was added into an aliquot of the reaction mixture to a final concentration of 2% (v/v). Following centrifugation at 16,100 RCF for 5 min using a bench-top microfuge, the supernantant was filtered through a 0.2 μm nylon membrane filter. Samples were then diluted 20- to 100-fold with water prior to analysis by LC/MS/MS.

D-Tryptophan Transamination Assay

To compare the D-tryptophan transamination activities of certain D-aminotransferases, the following assays were performed. The assay mix contained: 0.5 mg/mL of cellular extract protein containing D-AT; 40 mM potassium phosphate pH 8.0; 20 mM D-tryptophan; 100 mM sodium pyruvate; and 50 μM PLP. The assays were incubated at 37° C. for 30 minutes and then placed on ice.

The extent of reaction was followed by measuring the amount of indole-3-pyruvate formed using the following assay: to 5 μl, 10 μl and 20 μl of reaction mix, 200 μl of the following solution was added: 0.5 mM sodium arsenate; 0.5 mM EDTA; and 50 mM sodium tetraborate (pH 8.5). Absorbance of the indole-3-pyruvate enol-borate complex at 325 nm was compared to a standard curve of indole-3-pyruvate prepared in the same solution.

Alanine formation can also be used to follow the extent of the D-tryptophan transamination reactions. Alanine concentrations were determined as described in Example 3.

R,R Monatin Transamination Assay

Assay conditions (final volume 2 mL) included: 0.01% Tween; 100 mM EPPS pH 8.2; 100 mM sodium pyruvate; approximately 3 mM R,R monatin; 0.5 mg/mL DAT; and 50 μM PLP. The extent of reaction was monitored by detection of alanine or R-MP formed using the protocols described in Example 3.

Example 6 Method for Obtaining DATs and an Aldolase

This method described the cloning of the aldolase used in monatin formation reactions with the D-aminotransferases, and D-aminotransferases previously isolated that were used for comparative purposes.

Aldolase

The aldolase used in monatin production assays from D-tryptophan was isolated and subcloned into pET vectors as described in WO 2007/103389 (referred to in that application as the aldolase of SEQ ID NO:276 encoded by the nucleic acid of SEQ ID NO:275).

DAT and DAT4978 T243N

A D-aminotransferase from ATCC #4978 (DAT 4978) was cloned as described in U.S. Publication No. 2006/0252135. A T243N mutant was made using the pET30 (untagged) DAT 4978 construct.

The primer for mutagenesis was designed following the suggestions listed in the Stratagene Multi-Change kit (La Jolla, Calif.). The primer was 5′-phosphorylated. Mutagenesis was done using the Stratagene Multi-Change kit following the manufacturer's instructions. The mutagenic oligonucleotide sequence is shown in Table 8.

TABLE 8 Mutagenic oligonucleotide sequences Mutant name Amino acid change Primer SEQ ID NO: DAT4978T243N T243N 5′-GTGATTGTTTCATCAACGAATTCAGAAGTAACGCC-3′ 1062

E. coli XL10-Gold cells (Stratagene) were transformed and the resultant purified plasmid preparations were sequenced to verify that the correct mutations were incorporated. The plasmid containing the DAT 4978 T243N was then transformed into E. coli BL21 (DE3) expression host B. sphaericus DAT

A D-aminotransferase from B. sphaericus (ATCC number 10208) was cloned as described in US 2006/0252135. The protein was prepared as described in the same reference.

Example 7 Analysis of DATs

DAT polypeptides having the sequence shown in SEQ ID NO:928, 930, 932, 934, 936, 938, 940, 942, 944, 946, 948, and 950 were produced by expressing the corresponding nucleic acid in the vectors and in the compatible E. coli expression hosts described in Example 2. One skilled in the art can synthesize the genes encoding these D-aminotransferases using assembly PCR techniques such as those described in Example 4. Overnight cultures in LB medium containing carbenicillin (100 μg/mL) were diluted 100× in 100 mL of the same medium and grown in a 500 mL baffled flask. The culture was grown at 30° C. to an OD₆₀₀ of 0.5 to 0.9, and protein expression was induced with 1 mM IPTG for 4 h at the same temperature. Samples for total protein were taken immediately prior to harvesting. Cells were harvested by centrifugation and washed once with 10 mL of potassium phosphate buffer pH 7.8. Cells were immediately frozen at −80° C. until cell extracts were prepared.

Cell extracts were prepared and desalted as described in Example 4 using 100 mM potassium phosphate as the buffer to elute and equilibrate the PD10 column. Total protein and DAT concentrations were determined as described.

Transamination of R,R monatin with pyruvate as the amino acceptor were performed as described in Example 5 except that 10 mM R,R monatin was utilized. Initial analyses of alanine, monatin, and monatin precursor levels were not consistent with each other and results were considered qualitative. The DAT polypeptide having the sequence of SEQ ID NO:948 appeared to show monatin precursor formation.

For further confirmation of activity, a monatin formation assay was done as described in the methods with a DAT concentration of approximately 0.2 mg/mL. As a control, 0.2 mg/mL concentration of purified B. sphaericus DAT was evaluated. After 2 and 21 hr, an aliquot was taken and formic acid was added to a final concentration of 2%, and the samples were frozen. Samples were then thawed, spun and filtered. Samples were analyzed for monatin using LC/MS/MS methodology and for tryptophan and alanine using the LC/OPA post-column fluorescence methodology described in Example 3. The DAT polypeptides having the sequence of SEQ ID NO:946 and 950 were capable of R,R monatin formation under the conditions tested. The DAT polypeptide having the sequence of SEQ ID NO:948 showed a loss of tryptophan and an increase in alanine formation, demonstrating its activity as a D-tryptophan transaminase. The DAT polypeptide having the sequence of SEQ ID NO:946 expressed well as determined by the amount of total protein but was not very soluble, which explains some inconsistent results. The DAT polypeptides having the sequence shown in SEQ ID NO:930, 932, 940, 942, and 944 did not yield visible bands on analysis with SDS-PAGE and, therefore, may be active if produced under different conditions. See Table 9 for results.

TABLE 9 Activity of DATs Monatin [mM] Monatin [mM] DAT Polypeptide (SEQ ID NO) Time = 2 hr Time = 21 hr 928 nd nd 930 nd nd 932 nd nd 934 nd nd 936 nd nd 938 nd nd 940 nd nd 942 nd nd 944 nd nd 946 0.1 0.6 948 nd nd 950 0.4 3.1 B. sphaericus control DAT 0.8 4.4 nd = not detected under conditions tested Analysis of DAT Polypeptides in pET30a

The DAT polypeptides having the sequence of SEQ ID NO:946, 948, and 950 were subcloned into pET30a as described in Example 4. Duplicate cultures of E. coli strain BL21 DE3 containing the DATs in pET30a were grown overnight in Overnight Express II (Solution 1-6, Novagen) at both 25 and 30° C. As a control, a strain containing pET30a plasmid without an insert was also grown. Cells were collected at an OD₆₀₀ of 5-10. Cells were harvested by centrifugation and washed once with 10 mL of 100 mM potassium phosphate buffer pH 7.8. Cells were frozen at −80° C. until further processed.

Cell extracts were prepared as described in Example 4 using 100 mM potassium phosphate as the buffer to elute and equilibrate the PD10 columns. Total protein and DAT protein concentrations were determined as described. The DAT polypeptide having the sequence of SEQ ID NO:946 expressed well at 30° C. in the total protein fraction, but was not soluble as viewed by SDS-PAGE. The DAT polypeptides having the sequence of SEQ ID NO:948 and 950 expressed at the higher temperature also, but were soluble.

A monatin formation assay was done as described in Example 5 except with a DAT concentration of 0.1 mg/mL for the polypeptide of SEQ ID NO:946 (0.5 mg/mL for all others). As a positive control, purified B. sphaericus DAT at a 0.5 mg/mL concentration was also assayed. After 2 and 21 hr, an aliquot was taken, formic acid was added to a final concentration of 2%, and the samples were frozen until further processed. Samples were then thawed, spun and filtered. Samples were analyzed for monatin using LC/MS/MS, and for tryptophan and alanine using LC/OPA post-column fluorescence detection methods described in Example 3. The results are shown in Table 10. D-tryptophan consumption and alanine formation were shown for all the D-aminotransferases tested indicating that they all have activity on D-tryptophan. Under these conditions, only DAT polypeptides having the sequence of SEQ ID NO:946 and 948 appeared to have activity for monatin formation. It is possible that expression or stability differences between the two host systems are the reason why activity is seen in some cases but not in others.

TABLE 10 Monatin [mM] Monatin [mM] DAT polypeptide (SEQ ID NO) time = 2 hr time = 21 hr pET30 (negative control) nd nd 946 0.4 1.8 948 nd 0.2 950 nd nd B. sphaericus positive control 1.8 8.6 nd, not detected under conditions tested

Example 8 Analysis of DATs in pSE420-cHis

DAT polypeptides having the sequence shown in SEQ ID NOs:886, 888, 890, 892 and 894 DATs were produced from the pSE420-cHis vector in E. coli HMS174. One skilled in the art can synthesize the genes encoding these D-aminotransferases using assembly PCR techniques such as those described in Example 4. Overnight cultures of the various DAT constructs were grown in LB medium containing ampicillin (100 μg/mL) at 30° C. Fifty mL of the same medium was inoculated the next day with 1 mL of the overnight cultures. The cultures were grown at 30° C. until the OD_(600 nm) reached approximately 0.5 and then induced with 1 mM IPTG. The cultures were further incubated for 4 h at 30° C. and then harvested by centrifugation at 3800 rcf for 15 min. The cells were washed with 1.5 mL of 50 mM potassium phosphate, pH 7.0 and centrifuged again. The supernatant was decanted and the cell pellets were weighed.

Cell extracts were prepared as described in the methods using 100 mM potassium phosphate as the buffer to elute and equilibrate the column. Total and DAT concentrations were determined as described except BCA (Pierce) was used instead of Bradford for total protein determination. Two different vector only cultures were grown in the same E. coli hosts as the cloned DATs. All of the proteins produced visible bands on SDS-PAGE gels, but to differing degrees of solubility. Polypeptides having the sequence of SEQ ID NO:892 were not very soluble.

To compare the D-tryptophan transamination activities of each of the enzymes, the D-tryptophan transamination assay and the R,R monatin transamination assay described in Example 5 were performed. The D-tryptophan aminotransferase targeted using a final concentration of 0.5 mg/mL of cellular extract containing D-aminotransferase and 0.1 mg/mL of the purified B. sphaericus DAT as a control. Quantification of the DATs in the cellular extracts was difficult due to the low levels of soluble polypeptides. The DAT polypeptides having the sequence shown in SEQ ID NO:888, 892 and 894 showed good activity with D-tryptophan as a substrate during the 30 minute reaction. DAT polypeptides having the sequence shown in SEQ ID NO:886 and 890 had measurable activity above the no-enzyme control, but exhibited little activity under the conditions tested.

Monatin transamination experiments were performed at room temperature, taking samples after 0.5, 1 and 2 hours targeting 0.5 mg/mL of each DAT, including the purified positive control from B. sphaericus. The R,R monatin transamination samples were then analyzed for monatin and alanine. The amount of monatin remaining was quantified by LC/MS/MS; alanine formation was measured using the post-column derivatization method in Example 3. Under the conditions tested, the DAT polypeptides having the sequence shown in SEQ ID NOs:892 and 894 were active. The DAT polypeptide having the sequence of SEQ ID NO:894 appeared to have the highest activity for conversion of R,R monatin to R-MP. The trends were consistent when alanine formation was assayed. The alanine production numbers (in mM) for the various timepoints are shown in Table 11.

TABLE 11 Alanine formation (mM) from R,R monatin transamination reactions DAT polypeptide (SEQ ID NO) 0.5 hr 1 hr 2 hr vector control 1 0.139 0.185 0.215 vector control 2 0.179 0.242 0.301 886 0.128 0.203 0.242 888 0.13 0.203 0.275 890 0.112 0.153 0.176 892 1.034 1.587 2.167 894 2.2 2.52 2.663 BsphDAT(purified) 0.287 0.519 0.894 no enzyme 0.043 0.035 0.037

DAT nucleic acids having the sequence shown in SEQ ID NO:891 and 893 were subcloned into pET30 as described in Example 4. These constructs were transformed into a variety of E. coli hosts carrying the DE3 lysogen for expression from a T7 promoter, including both K-12 and B strains of E. coli, and one strain that carried the pLysS plasmid. The clones were expressed in OvernightExpress System II as described in Example 4, with and without the addition of 0.5 mM pyridoxine, and analyzed by SDS-PAGE or Experion for expression. From these experiments, it became apparent that the proteins were expressing mostly in the insoluble fraction. Pyridoxine helped improve solubility to a small degree as did lowering the temperature from 37 to 30° C. for induction. Further work was done in cloning systems designed to maximize soluble expression (see Example 16-22).

Example 9 Analysis of CaDAT, CbDAT, and LsDAT in pET30a

The amino acid sequence shown in SEQ ID NO:894 was used to search for similar proteins available in the public databases. Three DATs were found that had similarity to SEQ ID NO:894. They were from Lactobacillus salivarus (47% identical at the protein level), Clostridium beijerinckii (57% identical at the protein level), and Clostridium acetobutylicum (60% identical at the protein level). The gene and protein sequences and their accession numbers are shown at the end of this example. FIG. 1 is an alignment showing the consensus regions of these SEQ ID NO:894-like proteins. One can see a high degree of consensus regions indicating structural similarities.

These nucleic acids were cloned into pET30a, and the corresponding polypeptides expressed and tested for activity as described herein.

CbDAT

The D-aminotransferase from Clostridium beijerinckii (CbDAT) was cloned into pET30a (untagged) BL21 (DE3) and expressed using Overnight Express II (Novagen). The cells were collected at an optical density at 600 nm of approximately 9 and centrifuged at 4000 rcf for 15 min. The cells were washed once with 100 mM potassium phosphate pH 7.8 (cold), and spun again.

Cell extracts were prepared as described herein using 100 mM EPPS pH 8.2 as the buffer to elute and equilibrate the column. Total protein and DAT protein concentrations were determined as described except the BCA method (Pierce) was used instead of the Bradford (Coomassie) assay. The CbDAT expressed well but was only partially soluble.

A monatin formation assay was done as described in Example 5 but the activity of CbDAT (0.5 mg/mL) was also studied at pH 7.4 (with potassium phosphate as a buffer). As a control, purified B. sphaericus DAT (1 mg/mL) was assayed at pH 8.2. After 1, 2, 4, 8, and 23 hrs, aliquots were taken and formic acid was added to a final concentration of 2%. Samples were frozen at −80° C. until analyzed. Samples were then thawed, spun and filtered. Samples were analyzed for monatin using the LC/MS/MS methodology described in Example 3. Results are shown in Table 12. The amount of monatin produced were slightly higher for the assays carried out at pH 8.2. Similar experiments were performed with the polypeptides expressed from pET28 with an N-terminal His-tag. The activity of the tagged version appeared to be slightly less than that of the untagged, but still easily detectable.

TABLE 12 Activity over time Monatin Monatin Monatin Monatin Monatin (ppm) (ppm) (ppm) (ppm) (ppm) DAT Enzyme 1 hr 2 hr 4 hr 8 hr 23 hr CbDAT pET30 45 126 280 428 502 (7.4 mg/mL) CbDAT pET30 67 189 344 436 568 (8.2 mg/mL) B. sphaericus 531 968 1742 2310 3706 DAT (1 mg/mL) CaDAT and LsDAT

The D-aminotransferases from Lactobacillus salivarus (LsDAT) and Clostridium acetobutylicum (CaDAT) were cloned into pET30a (untagged) BL21(DE3) and expressed using Overnight Express II (Novagen). The cells were collected when the culture reached an optical density at 600 nm of approximately 9 by centrifugation at 4000 rcf for 15 minutes.

Cell extracts were prepared as described herein using 100 mM EPPS pH 8.2 as the buffer to elute and equilibrate the column. Total protein and DAT protein concentrations were determined using the BCA (Pierce) protocol. Both enzymes expressed well and were soluble.

The assay was performed at room temperature under anaerobic conditions. As a control, purified B. sphaericus D-aminotransferase was assayed. Approximately 0.5 mg/mL of each DAT was used. After 0.5, 1, 2, 4, 6, 8 and 22 hr an aliquot was taken and formic acid added to a final concentration of 2% and the samples were frozen. Samples were then thawed, spun and filtered. Samples were analyzed for monatin using the LC/MS/MS methodology described in Example 3. Results are shown in Table 13.

TABLE 13 Monatin Monatin Monatin Monatin Monatin Monatin DAT (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) Monatin (ppm) polypeptide 0.5 hr 1 hr 2 hr 4 hr 6 hr 8 hr 22 hr B. sphaericus 76.6 194.4 457.6 860.8 1186 1770 2546 LsDAT 2.8 6.4 14.6 33 52 69.8 173 CaDAT 50.2 141.2 318.6 543.4 612 1144 668

The homologs of the DAT having the sequence shown in SEQ ID NO:894 were active. Since the homologs showing the conserved sequence above were all active in monatin formation assays, it is expected that any D-aminotransferase containing the consensus sequences described herein would also be active, although their primary sequence identity is as low as 47%. There has been no evidence before this work that these unique D-aminotransferases, with low homology to the more characterized Bacillus D-aminotransferase, would have activity for monatin or would be broad specificity enzymes.

DNA Sequence CaDAT (ACCESSION AE001437 AE007513-AE007868; VERSION AE001437.1 GI: 25168256; nucleotides 914049 . . . 914891) (SEQ ID NO: 1063) 1 atgaaagatt taggatatta caatggagaa tacgacttaa ttgaaaatat gaaaatacca 61 atgaatgatc gtgtatgcta ttttggtgat ggtgtttatg atgctactta tagtagaaac 121 cataatatat ttgcactaga tgagcatatt gaccgatttt ataatagtgc cgagctttta 181 agaattaaaa ttccatatac aaagaaggaa atgaaagagc ttttaaagga tatggttaaa 241 aaggttgata gcggagaaca atttgtatat tggcaggtta ctagaggtac tggcatgcgt 301 aatcatgctt ttttgagtga ggatgttaag gctaatattt ggattgtttt aaagccacta 361 aaggtaaaag atatgtcaaa aaaattaaaa ctaataacat tagaggatac tagattttta 421 cattgtaaca taaaaacctt aaatttgctt cctagtgtaa ttgcagcaca aaaaactgaa 481 gaagcaggct gccaggaagc agtatttcat agaggagata gagttactga atgtgctcat 541 agtaatgttt caattataaa ggatgagatt ttaaaaactg cgccaacaga taatcttatt 601 ttgccgggaa tagcaagggc gcatcttata aaaatgtgca aaaaatttga gatacctgta 661 gatgaaactc catttacatt aaaggagtta attaatgcgg atgaagttat agttacaagt 721 tcagggcaat tttgtatgac tgcttgtgag atagatggaa gacctgtagg cggaaaagcg 781 ccagatatta ttaaaaagct tcagactgcc ttacttaatg aatttttgga agaaacaaat 841 taa Protein Sequence CaDAT (ACCESSION NP_347428; VERSION NP_347428.1 GI: 15894079) (SEQ ID NO: 1064) 1 MKDLGYYNGE YDLIENMKIP MNDRVCYFGD GVYDATYSRN HNIFALDEHI DRFYNSAELL 61 RIKIPYTKKE MKELLKDMVK KVDSGEQFVY WQVTRGTGMR NHAFLSEDVK ANIWIVLKPL 121 KVKDMSKKLK LITLEDTRFL HCNIKTLNLL PSVIAAQKTE EAGCQEAVFH RGDRVTECAH 181 SNVSIIKDEI LKTAPTDNLI LPGIARAHLI KMCKKFEIPV DETPFTLKEL INADEVIVTS 241 SGQFCMTACE IDGRPVGGKA PDIIKKLQTA LLNEFLEETN DNA Sequence CbDAT (ACCESSION CP000721 AALO01000000 AALO01000001- AALO01000089 VERSION CP000721.1 GI: 149901357; nucleotides 3213484 . . . 3212636) (SEQ ID NO: 1065) 1 atggagaatt taggttatta taatggaaag tttggattat tagaggaaat gacagtacca 61 atgcttgatc gtgtttgcta ttttggagat ggagtttatg atgctactta tagcagaaat 121 cacaagattt ttgcattgga ggagcatatt gaaagatttt acaacagcgc tggtttatta 181 ggaattaaaa ttccttattc aaaggagcaa gtaaaagaaa tccttaagga gatggtatta 241 aaggttgatt caggagaaca atttgtatat tggcaaatta ctagaggaac tggaatgaga 301 aatcatgctt ttcctggaga tgaggtccct gcaaatctat ggattatgtt aaagccttta 361 aatattaagg atatgtcaca aaaattaaag ttaatcactt tagaagacac tagattttta 421 cactgtaata tcaaaacctt aaatttatta ccaagtgtaa ttgcatctca aaaaactgaa 481 gaggcaggat gtcaggaagc tgtatttcat agaggggata gagtaactga atgtgcacat 541 agcaatgtat caattattaa ggatggtata ttaaaaactg ctccaacaga caatttaatt 601 ttaccaggta tagcaagagc tcaccttatt aaaatgtgta aatcctttaa tattcctgta 661 gatgaaacag catttacctt gaaggaatta atggaggcag atgaagttat agttactagt 721 tcaggtcaat tttgtatggc aaccagtgaa atagatggaa tacctgtagg gggaaaagca 781 ccagagcttg taaagaaatt acaagatgca ttgttaaatg agttcttaga agaaacaaaa 841 acagaatag Protein Sequence CbDAT (ACCESSION YP_001309869 VERSION YP_001309869.1 GI: 150017615) (SEQ ID NO: 1066) 1 MENLGYYNGK FGLLEEMTVP MLDRVCYFGD GVYDATYSRN HKIFALEEHI ERFYNSAGLL 61 GIKIPYSKEQ VKEILKEMVL KVDSGEQFVY WQITRGTGMR NHAFPGDEVP ANLWIMLKPL 121 NIKDMSQKLK LITLEDTRFL HCNIKTLNLL PSVIASQKTE EAGCQEAVFH RGDRVTECAH 181 SNVSIIKDGI LKTAPTDNLI LPGIARAHLI KMCKSFNIPV DETAFTLKEL MEADEVIVTS 241 SGQFCMATSE IDGIPVGGKA PELVKKLQDA LLNEFLEETK TE DNA Sequence LsDAT (ACCESSION CP000233 VERSION CP000233.1 GI: 90820184; nucleotides 1750082 . . . 1750927) (SEQ ID NO: 1067) 1 atgaagcaag ttggatacta caatggtact atcgctgatt taaatgaact taaggtgcct 61 gctactgatc gtgcacttta ttttggtgat ggttgctacg atgcaactac atttaagaac 121 aatgttgcat ttgccttaga agatcatctt gatcgttttt ataatagttg tcgcctacta 181 gagatcgatt tccctttaaa tcgcgatgaa cttaaagaaa agctttacgc tgttattgat 241 gctaacgaag ttgatactgg tatcctttat tggcaaactt cacgtggttc tggtttacgt 301 aaccatattt tcccagaaga tagccaacct aatttattaa tttttactgc tccttatggt 361 ttagttccat ttgatactga atataaactt atatctcgcg aagacactcg cttcttacat 421 tgcaatatta aaactttgaa tttacttcca aacgttattg caagtcaaaa ggctaatgaa 481 agtcattgcc aagaagtggt cttccatcgc ggtgacagag ttacagaatg tgcacactct 541 aacatcttaa ttctaaaaga tggcgttctt tgctccccac ctagagataa tttaatcttg 601 ccaggaatta ctttgaaaca tctcttgcaa ttagcaaaag aaaataatat tcctacttcc 661 gaagcaccat tcactatgga tgatcttaga aatgctgatg aagttattgt tagttcttca 721 gcttgtctag gtattcgcgc agtcgagctt gatggtcagc ctgttggtgg aaaagatgga 781 aagactttaa agatcttgca agatgcttat gctaagaaat ataatgctga aactgtaagt 841 cgttaa Protein Sequence LsDAT (ACCESSION YP_536555 VERSION YP_536555.1 GI: 90962639) (SEQ ID NO: 1068) 1 MKQVGYYNGT IADLNELKVP ATDRALYFGD GCYDATTFKN NVAFALEDHL DRFYNSCRLL 61 EIDFPLNRDE LKEKLYAVID ANEVDTGILY WQTSRGSGLR NHIFPEDSQP NLLIFTAPYG 121 LVPFDTEYKL ISREDTRFLH CNIKTLNLLP NVIASQKANE SHCQEVVFHR GDRVTECAHS 181 NILILKDGVL CSPPRDNLIL PGITLKHLLQ LAKENNIPTS EAPFTMDDLR NADEVIVSSS 241 ACLGIRAVEL DGQPVGGKDG KTLKILQDAY AKKYNAETVS R

Example 10 Analysis of DATs

HMS174 E. coli containing the DAT nucleic acids having the sequence of SEQ ID NO:865, 867, 869, 871, 873, 875, and 877 in vector pSE420-cHis were obtained and streaked on agar plates containing LB medium with ampicillin. One skilled in the art can synthesize the genes encoding these D-aminotransferases using assembly PCR techniques such as those described in Example 4. Single colonies were used to inoculate 3 mL of LB medium containing ampicillin (100 μg/mL). Five hundred μl of the overnight culture was used to inoculate 50 mL of the same medium in 250 mL baffled flasks. The cells were grown at 30° C. to approximately an OD_(600nm) of 0.5. IPTG was added to a final concentration of 1 mM. Cells were induced at 30° C. for 4 hours and collected by centrifugation.

Cell extracts were prepared as described in Example 4. Total protein and DAT concentrations were determined as described in Example 4. The DATs all appeared to express well, and most of them showed a high degree of solubility.

A monatin formation assay was done as described in Example 5 except with a DAT concentration of 0.1 mg/mL and the aldolase at a concentration of 10 μg/mL. As a control, 0.1 mg/mL of purified B. sphaericus DAT was assayed. After 6 and 22 hours, an aliquot was taken and formic acid added to a final concentration of 2%, and the samples were frozen. Samples were then thawed, spun and filtered. Samples were analyzed for monatin concentrations using the LC/MS/MS methodology described in Example 3. Under the conditions tested, SEQ ID NO:870, 874 and 878 all appeared to have high activity in the 3-step monatin formation assay. DAT polypeptides having the sequences shown in SEQ ID NOs:866, 872, and 876 also had activity in the monatin formation pathway but not to the same extent as did polypeptides having the sequence shown in SEQ ID NOs:870, 874 and 878 under the conditions tested. Table 14 shows the results for monatin formation (in ppm).

TABLE 14 Monatin formation assay DAT polypeptide (SEQ ID NO) 6 hr 22 hr 866 17.4 76 868 nd nd 870 132 836 872 13.8 50 874 281.6 798 876 2.4 12 878 223.4 576 B. sphaericus DAT 175.6 616 nd, not detected under conditions tested Further Analysis of Polypeptides Having the Sequence of SEQ ID NO:870, 874 and 878 in pET30a

Cultures of E. coli BL21 DE3 transformed with pET30a plasmids containing nucleic acids encoding the above-indicated DATs were grown overnight in 50 mL of Overnight Express II (Solution 1-6, Novagen) at 30° C. As a positive control, a strain containing the DAT from ATCC #4978 in pET30a was also grown and induced (described in Example 6). Cells were collected at an OD_(600nm) of 5-10, harvested by centrifugation and frozen at −80° C. until further processed.

Cell extracts were prepared as described in the Example 4. Total protein and DAT concentrations were determined as described in Example 4.

A monatin formation assay was done as described in Example 5 except with a DAT polypeptide concentration of 0.5 mg/mL for SEQ ID NO:870 and a concentration of 0.275 mg/mL for each of SEQ ID NO:874 and 878. As a control, DAT4978 and purified B. sphaericus DAT were assayed at 0.5 mg/mL concentration. After 0.5, 1, 2, 4, 6.5, 9, 24 and 22 hr, an aliquot was taken and formic acid added to a final concentration of 2% and the samples were frozen. Samples were then thawed, spun and filtered. Samples were analyzed for monatin using the LC/MS/MS methodology described in Example 3. The results are shown in Table 15 (in ppm of monatin formed).

TABLE 15 DAT polypeptide (SEQ ID NO) 0.5 hr 1 hr 2 hr 4 hr 6.5 hr 9 hr 24 hr 4978 DAT 18 85.6 283.4 673.2 890 1226 2020 870 14.4 71 279.4 736 1340 1680 3362 874 63.8 182.6 415.6 674 888 938 1154 878 97.8 244.4 607 912.2 1068 1174 1356 B. sphaericus 44.6 142.8 375.2 813 1294 1382 2746

All three of the subcloned DATs (encoding polypeptides having the sequence of SEQ ID NO:870, 874, and 878) expressed well in the pET system and yielded soluble protein. The polypeptide having the sequence shown in SEQ ID NO:870 gave the highest amount of expression in the soluble fraction and exhibited high activity that did not appear to diminish over time in comparison to the DAT polypeptides having the sequence of SEQ ID NO:874 and 878.

Comparison Between Wild Type and Mutant DAT Polypeptides

A mutant polypeptide in which the residue of SEQ ID NO:870 was changed from a T to a N (SEQ ID NO:870 T242N) was constructed as described in Example 4 and expressed and compared to DAT4978, DAT4978 T243N (described in Example 6), B. sphaericus and wildtype SEQ ID NO:870.

Cultures of BL21 DE3 in which the wild type and mutant polypeptides having the sequence of SEQ ID NO:870, 870 T242N, DAT4978 and DAT4978 T243N were expressed from the pET30a vector, were grown in 50 mL of Overnight Express (Novagen) in a 250 mL baffled flask overnight at 30° C. and 250 rpm. The cells were collected by centrifugation when they reached an optical density at 600 nm of over 10. Cell extracts were prepared as described in Example 4, and total protein and DAT concentrations were determined as described in Example 4. All of the DATs tested were highly expressed and soluble, all near 30% as determined using the Experion software. The polypeptide having the sequence of SEQ ID NO:870 T242N had the highest expression, which was predicted to be 36.3% of the total soluble protein.

A monatin formation assay was done as described in Example 5 at a DAT polypeptide concentration of 0.5 mg/mL. As a control, 0.5 mg/mL of purified B. sphaericus DAT was assayed. After 0.5, 1 2, 4, 6.5, 9 and 23.25 hr, an aliquot was taken, formic acid added to the aliquot to a final concentration of 2% and the samples frozen. Samples were then thawed, spun and filtered. Samples were analyzed for monatin, tryptophan, alanine and 4-hydroxy-4-methyl glutamic acid (HMG) as described in Example 3.

In the last time point, an additional aliquot was taken to determine % R,R monatin by the FDAA-derivatization method described in Example 3.

Monatin formation numbers (ppm) are presented in Table 16 below. The percent R,R is given in the right-hand column, for the 23.25 hr timepoint.

TABLE 16 DAT polypeptide(SEQ ID NO) 0.5 hr 1 hr 2 hr 4 hr 6.5 hr 9 hr 23.25 hr % R,R Wild type DAT 4978 11 57 216 472 694 942 1616 95.0 4978 T243N 74 237 542.6 1106 1396 1784 2202 99.0 870 15.6 74.4 269.6 702 1250 1522 2788 97.8 870 T242N 49.4 194 655.2 1496 2212 2666 3670 99.5 B. sphaericus 40.6 144 372 800 1090 1458 2434 97.2

The activity of the T242N mutant of the SEQ ID NO:870 polypeptide was very high, and was better than the positive controls and higher than the wildtype form of DAT polypeptides. The percentage of R,R monatin formed by this mutant was also higher than any of the other benchmark enzymes. The analysis of the amount of HMG (a by-product) formed is qualitative, but it appears that, at the 9 hour and 23.25 hour timepoints, similar amounts of HMG were formed by DAT 4978 T243N polypeptides and SEQ ID NO:870 T242N polypeptides.

The DAT polypeptide having the sequence shown in SEQ ID NO:870 is a novel protein, exhibiting 76% sequence identity to the closest known D-aminotransferase (Bacillus YM-1 D-aminotransferase) and 69% amino acid sequence identity to the B. sphaericus DAT described in Example 6. FIG. 2 shows an alignment of this novel enzyme with other published DATS, and one can see the residues that make this enzyme unique and may account for its superior activity.

The highly active DAT polypeptide having the sequence shown in SEQ ID NO:910 (more similar to B. sphaericus type DATs; see Example 12) is also shown in the alignment. As an example of the uniqueness of the SEQ ID NO:870 polypeptide, in the region surrounding amino acid residue 54-55 (B. sphaericus numbering) in the alignment of FIG. 2, it is clear that the Bacillus-like DATs have a high degree of conservation whereas SEQ ID NO:870 has the residues EC rather than AS. As another example, in the highly conserved region surrounding residue 135 of the alignment shown in FIG. 2, the SEQ ID NO:870 polypeptide has a more hydrophilic residue (T) versus predominantly valine residues. The core sequence that represents the SEQ ID NO:870 enzyme, but excludes previously known broad specificity D-aminotransferases and highly related homologs, is shown as Consensus Sequences A and B. It is expected that any polypeptide containing one of these consensus sequences would exhibit DAT activity and be active in monatin formation pathway steps.

Consensus Sequence A (SEQ ID NO: 1069) Y.*LWND.*IV.*EDRGYQFGDG.*YEV.*KVY.*G.*FT.*EH.*DR.*YECAEKI.*PYTK.*H.*L LH.*L.*E.*N.*TG.*YFQ.*TRGVA.*RVHNFPAGN.*Q.*V.*SGT.*K.*F.*R.*N.*KGVKAT.* TED.*RWLRCDIKSLNLLGAVLAKQEAIEKGCYEA.*LHR.*G.*TE.*SS.*N.*GIK.*GTLYT HPA.*N.*ILRGITR.*V.*TCAKEIE.*PV.*Q.*T.*K.*LEMDE.*V.*S.*SE.*TP.*I.*DG.*KI.*N G.*G.*WTR.*LQ.*F.*K.*P. Consensus Sequence B (SEQ ID NO: 1070) Y[ST]LWND[QK]IV.[DE].{2}[VI].[IV].{2}EDRGYQFGDG[IV]YEV[IV]KVY[ND]G.[ML]F T.{2}EH[IV]DR.YECAEKI[RK][LIV].[IV]PYTK.{3}H[QK]LLH.L[VI]E.N.[LV].TG[HN][IVL] YFQ[IV]TRGVA.RVHNFPAGN[VI]Q.V[LI]SGT.K.F.R.{3}N.[EQ]KGVKAT.TED[IV]RWL RCDIKSLNLLGAVLAKQEAIEKGCYEA[IV]LHR.G.[VI]TE.SS.N[VI][FY]GIK[DN]GTLYT HPA[ND]N.ILRGITR.V[IV][LI]TCAKEIE[LMI]PV.[EQ]Q.{2}T.K.{2}LEMDE[LIVM].V[ST] S.[TS]SE[IV]TP[VI]I[DE][IVL]DG.KI.NG.{2}G[ED]WTR[KQ]LQ.{2}F.{2}K[IL]P..

Similar to PERL regular expression convention language, “.*” indicates that any number of amino acid residues may be present from any of the 20 proteinogenic amino acids; [ ] indicates that any one of the amino acids in the brackets can be present; “.{#}” indicates that any of the 20 proteinogenic amino acids can be present as long as the number of residues matches the number {#} indicated in the brackets.

With respect to the use of “.*” in Consensus sequence A, the number of amino acids at any of the “.*” positions can vary, for example, from 0 to about 20 residues (see, for example, Consensus sequence B (SEQ ID NO:1070)) or from about 20 residues up to about 100 residues, or the number of amino acids can be much larger, for example, up to 1000 or more residues. Without limitation, an insertion at one or more of the “.*” positions can correspond to, for example, a domain such as (but not limited to) a chitinase binding domain (e.g., from Pyrococcus furiosus (Accession No. 2CZN_A) or P. burkholderia (Accession No. YP_(—)331531) or a cellulose binding domain (e.g., from Cellulomonas fimi (Accession No. 1 EXH_A) or Clostridium stercorarium (Accession No. 1UY1_A). In some embodiments (without limitation), five or less of the positions designated “.*” each contain an insertion of, for example, greater than about 20 residues (e.g., greater than about 100 residues). In other embodiments (without limitation), five or more of the positions designated “.*” each contains an insertion of less than about 100 residues (e.g., less than about 20 residues, e.g., 3, 5, 10, 20, 25, 30, 40, 50, 60, 70, 75, 80, 90 or 95 residues)). The activity of a polypeptide having a sequence that corresponds to one or more of the consensus sequences disclosed herein and containing any number of residues inserted at one or more of the “.*” positions can be evaluated using methods that are described herein.

Non-limiting representative polypeptides that contain the consensus sequence shown in SEQ ID NO:1069 include the polypeptide having the sequence shown in SEQ ID NO:870 and Consensus sequence B (SEQ ID NO:1070).

Comparison Between SEQ ID NO:870, 870 T242N, DAT 4978 and DAT 4978 T243N, Tagged and Untagged

E. coli BL21 DE3 cells expressing the polypeptides having the sequence of SEQ ID NO:870, 870 T242N, DAT 4978 and DAT 4978 T243N were grown in 50 mL of Overnight Express (Novagen) in a 250 mL baffled flask overnight at 30° C. and 250 rpm. The cells were collected by centrifugation when the optical density at 600 nm was greater than 10. Cell extracts were prepared as described in Example 4. Total protein and DAT concentrations were determined as described in Example 4.

E. coli BL21 DE3 cultures expressing C-terminal tagged SEQ ID NO:870, 870 T242N, DAT 4978 and DAT 4978 T243N polypeptides were grown in 200 mL of Overnight Express (Novagen) in a 1000 mL baffled flask overnight at 30° C. and 250 rpm. Two cultures of each clone were grown. The cells were pooled and collected by centrifugation when at an optical density at 600 nm of over 10. Cell extracts were created by the addition of 50 mL of Bug Buster Primary Amine Free (Novagen) with 50 μl of Benzonase Nuclease (Novagen), 0.75 μl of rLysozyme (Novagen), and 250 μl of Protease Inhibitor Cocktail II (Calbiochem). The cells were incubated for 15 minutes at room temperature with gentle rocking. The extracts were centrifuged at 45,000×g for 10 minutes.

The His-tagged proteins were purified as described in the methods section using GE Healthcare (Piscataway, N.J.) Chelating Sepharose™ Fast Flow resin. The purified protein was desalted using a PD10 column into 100 mM potassium phosphate, pH 7.8 (for DAT 4978 and DAT 4978 T243N polypeptides) or 100 mM EPPS pH 8.2 (for SEQ ID NO:870 and 870 T242N polypeptides), both buffers contained 50 μM PLP.

An R,R monatin formation assay was performed as described in Example 5 with a DAT concentration of 0.5 mg/mL. Aliquots were taken at 2.25, 4.5, 9 and 24 hours, pH adjusted with formic acid, and frozen. An extra aliquot was taken at the final time point without formic acid addition for determination of stereoisomeric distribution using the FDAA derivatization method described in Example 3. The samples were thawed and centrifuged for 5 minutes and the supernatant filtered with a 0.2 μm nylon membrane filter. Samples were submitted for monatin analysis using the LC/MS/MS method described in Example 3. The results are shown in Table 17, in ppm monatin formed. The far right column is the % R,R monatin formed at the end of the experiment.

TABLE 17 DAT polypeptide (SEQ ID NO) 2.25 hr 4.5 hr 9 hr 24 hr % R,R DAT 4978 330 676 1470 2384 92.3 DAT 4978 T243N 1392 2856 4068 3688 98.2 870 395 952 1896 2998 97.8 870 T242N 1416 2936 3868 3976 99.3 DAT 4978 6xHis tagged 362 887 1664 2818 96.5 DAT 4978 T243N 6xHis tagged 1364 2298 3464 4440 98.9 870 6xHis tagged 228 688 1508 3138 98.1 870 T242N 6xHis tagged 746 2020 3962 4552 99.5

The overall activity and stereospecificity of the C-terminally tagged and untagged enzymes are very similar. In addition, it is expected that the presence of activity in a polypeptide encoded from a subcloned nucleic acid is predictive of the presence of activity in the corresponding polypeptide encoded from the full-length or wild type nucleic acid.

Example 11 Analysis of DATs

E. coli HMS174 containing DAT nucleic acids in the pSE420-cHis vector encoding the polypeptides having the sequence of SEQ ID NO:880, 882, and 884 were streaked onto agar plates containing LB medium with ampicillin. One skilled in the art can synthesize the genes encoding these D-aminotransferases using assembly PCR techniques such as those described in Example 4. Single colonies were used to innoculate 3 mL of LB medium containing ampicillin (100 μg/mL). Five hundred μl was used to inoculate 50 mL of the same medium in a 250 baffled flask. The cells were grown at 30° C. to approximately an OD_(600nm) of 0.4, and IPTG was added to a final concentration of 1 mM. Cells were grown at 30° C. for 4 hours and collected by centrifugation.

Cell extracts were prepared as described in the Example 4. Total protein and DAT polypeptide concentrations were determined as described in Example 4. SEQ ID NO:882 and 884 expressed well and were present at high levels in the soluble fraction.

An R,R monatin formation assay was performed as described in Example 5 using approximately 0.5 mg/mL of each DAT polypeptide (except that 0.35 mg/mL of the SEQ ID NO:880 polypeptide was utilized). After 2, 8, and 23 hours, an aliquot was taken, formic acid was added to a final concentration of 2%, and the samples were frozen. Samples were then thawed, spun and filtered. Samples were analyzed for monatin using the LC/MS/MS methodology described in Example 3. Results are shown in Table 18.

At the last time point, an extra aliquot was taken (without pH adjustment) to determine the stereoisomeric distribution of the monatin produced using the FDAA derivatization methodology described in Example 3. The percentage of R,R produced is shown in the right hand column of Table 18 below, the balance is predominantly S,R monatin.

TABLE 18 Polypeptide monatin ppm monatin ppm monatin ppm % R,R (SEQ ID NO) (2 hr) (8 hr) (23 hr) (23 hr) 880 31.6 140 176 97.5 882 31.6 872 2790 99.3 884 79.4 644 1610 100 B. sphaericus 337 1518 2538 96.7

Polypeptides having the sequence shown in SEQ ID NO:882 and 884 exhibited good activity in the monatin formation reactions from D-tryptophan.

The stereopurity of the monatin produced was higher when using these DATs as compared to the B. sphaericus control enzyme. The DAT nucleic acids encoding DAT polypeptides having the sequence of SEQ ID NO:882 and 884 were subcloned into pET30a vectors as described in Example 4.

Analysis of DAT Polypeptides Having the Sequence of SEQ ID NO:882 and 884 Expressed from the pET30a Vector

Cultures of E. coli BL21 DE3 containing nucleic acids encoding DAT polypeptides having the sequence of SEQ ID NO:882 and 884 in the pET30a vector were grown in 50 mL of Overnight Express (Novagen) in a 250 mL baffled flask overnight at 30° C. and 250 rpm. The cells were collected by centrifugation when the optical density at 600 nm was greater than 10.

Cell extracts were prepared as described in Example 4. Total protein and DAT polypeptide concentrations were determined as described. Total and soluble protein samples were analyzed using a 4-15% gradient acrylamide gel as well as by the Experion system. Expression was predicted to be approximately 30% by the Experion software. Visible bands were seen for both the total protein and soluble protein (cell-free extract) fractions.

A monatin formation assay was performed as described in Example 5 with both 0.5 and 2 mg/mL DAT polypeptide concentrations. Purified B. sphaericus DAT was used as a control. After 2, 4.5, 9, 24, 36 and 48 hrs, an aliquot was taken, formic acid was added to a final concentration of 2%, and the samples were frozen. Samples were then thawed, spun and filtered. Samples were analyzed for monatin using the LC/MS/MS methodology described in Example 3. The samples were qualitatively analyzed for HMG levels. Additional aliquots were taken (without pH adjustment) for stereoisomeric analysis using the FDAA derivatization methodology described in Example 3. The results are shown in Tables 19 and 20.

TABLE 19 DAT Monatin Monatin Monatin Monatin Monatin Monatin polypeptide (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (SEQ ID NO) 2 hrs 4.5 hrs 9 hrs 24 hrs 36 hrs 48 hrs 882 61 274 780 1802 2172 2170 (0.5 mg/mL) 882 (2 mg/mL) 985 2452 3232 3128 3082 3158 884 149 362 656 1394 1756 2158 (0.5 mg/mL) 884 (2 mg/mL) 811 1628 2466 2988 3178 2864 B. sphaericus 362 860 1268 2362 2532 2804 (0.5 mg/mL) B. sphaericus 1335 2344 3154 3866 3842 4008 (2 mg/mL)

TABLE 20 Stereopurities of monatin produced at selected timepoints DAT polypeptide 24 hrs 48 hrs (SEQ ID NO) (% R,R) (% R,R) 882 (2 mg/mL) 95.4 94.1 884 (2 mg/mL) 99.6 99.4 Bs DAT (2 mg/mL) 95.8 93.3

Polypeptides having the sequence shown in SEQ ID NO:882 and 884 exhibited good monatin formation activity and stereospecifity, and appeared to produce less HMG than the B. sphaericus control during the initial timepoints. Polypeptides having the sequence of SEQ ID NO:882 exhibited similar initial monatin formation rates but appeared to have plateaued in this experiment at a lower monatin titer.

Example 12 Analysis of DATs in pSE420-cHis, and of a DAT in pET30a

The open reading frames encoding DAT polypeptides having the sequence of SEQ ID NO:898, 900, 902, 904, 906, 910, and 896 were evaluated. One of ordinary skill in the art can synthesize the genes encoding these D-aminotransferases using assembly PCR techniques such as those described in Example 4.

A culture of E. coli BL21 DE3 containing a nucleic acid encoding a DAT polypeptide having the sequence shown in SEQ ID NO:896 in the pET30a vector (subcloned as described in Example 4) was grown in 50 mL of Overnight Express (Novagen) in a 250 mL baffled flask overnight at 30° C. and 250 rpm. The cells were collected by centrifugation when the optical density at 600 nm was greater than 10.

Top10 (Invitrogen, Carlsbad, Calif.) E. coli cells were transformed with the pSE420-cHis plasmid containing the DAT nucleic acids having the sequence shown in SEQ ID NO:897, 899, 901, 903, 905, and 909 and plated on LB medium containing ampicillin (100 μg/mL). Five hundred μl of an overnight culture was used to inoculate 50 mL of the same medium in a 250 baffled flask. The cells were grown at 30° C. to an OD_(600nm) of approximately 0.4 and IPTG was added to a final concentration of 1 mM. Cells were grown at 30° C. for 4 hours and collected by centrifugation.

Cell extracts were prepared as described in Example 4. Soluble protein and estimated DAT concentrations were determined as described in Example 4.

An R,R monatin formation assay was performed as described in Example 5 with DAT polypeptide concentrations of 0.5 mg/mL, except that 0.3 mg/mL of the polypeptide having the sequence of SEQ ID NO:896 was used; 0.06 mg/mL of the polypeptide having the sequence of SEQ ID NO:898 was used; 0.4 mg/mL of the polypeptide having the sequence shown in SEQ ID NO:900 was used; 0.1 mg/mL of the polypeptide having the sequence of SEQ ID NO:902 was used; and 0.12 mg/mL of the polypeptide having the sequence of SEQ ID NO:904 was used. As positive controls, SEQ ID NO:870, 870 T242N, and purified B. sphaericus were tested at DAT polypeptide concentrations of 0.5 mg/mL. After 2, 6, and 24 hours, an aliquot was taken, formic acid was added to a final concentration of 2% and the samples were frozen. Samples were then thawed, spun and filtered. Samples were analyzed for monatin using the LC/MS/MS methodology described in Example 3. Additional aliquots were taken for stereoisomeric distribution analysis and were not treated with formic acid. The results for the 24 hour time point are shown in Table 21. The DAT nucleic acid encoding the DAT polypeptide having the sequence shown in SEQ ID NO:908 was not subcloned and could not be assayed.

TABLE 21 Polypeptide (SEQ monatin monatin monatin 24 hr ID NO) 2 hrs (ppm) 6 hrs (ppm) 24 hrs (ppm) % R,R B. sphaericus 261 1203 2604 95.6 870 193 1067 2490 96.8 870 T242N 813 2230 3380 98.8 896 30 127 286 99.8 898 nd 3 15 95.7 900 4 16 56 92.9 902 144 411 1209 96.7 904 nd 1 4 92.3 906 14 18 25 98 910 487 1154 2770 94.5 nd = not detectable under conditions tested

DAT polypeptides having the sequence shown in SEQ ID NO:910 and 902 had high levels of activity for the monatin formation reactions, and produced fairly high levels of R,R monatin. Results indicated that the DAT polypeptide having the sequence shown in SEQ ID NO:870 exhibited comparable activity to that of the wildtype polypeptide having the sequence shown in SEQ ID NO:910 under the conditions tested; however, the T242N mutation in the SEQ ID NO:870 polypeptide makes a large improvement in activity and stereospecificity of the enzyme.

Example 13 Analysis of DATs

Plasmids (pSE420-cHis) containing the nucleic acid sequences encoding SEQ ID NO:912, 914, 916, 918, 920, 922, 924, and 926 DATs were obtained. One skilled in the art could clone the genes using any number of gene assembly protocols such as the one described in Example 4.

E. coli Top10 (Invitrogen) cells were transformed with the pSE420-cHis plasmids containing DAT polypeptides having the sequence of SEQ ID NO:912, 914, 916, 918, 920, 922, 924, and 926 and plated on LB medium containing ampicillin (100 μg/mL). Five hundred μl of the overnight culture was used to inoculate 50 mL of the same medium into 250 mL baffled flasks. Cultures were grown at 30° C. to an OD_(600nm) of approximately 0.4. IPTG was added to a final concentration of 1 mM. Cells were grown at 30° C. for 4 hours and collected by centrifugation.

Cell extracts were prepared as described in Example 4. Total soluble protein and DAT protein concentrations were determined as described in Example 4. Most of the DAT polypeptides that were expressed were soluble except for the SEQ ID NO:916 polypeptide, which was only partially soluble.

An R,R monatin formation assay was performed as described in Example 5, with a DAT polypeptide concentration targeted to about 0.25 mg/mL; except that 0.1 mg/mL of the SEQ ID NO:922 polypeptide was used and 0.2 mg/mL of the SEQ ID NO:926 polypeptide was used. As a positive control, purified B. sphaericus DAT was tested at a 0.25 mg/mL concentration. After 2, 8 and 24 hours, an aliquot was taken and formic acid was added to a final concentration of 2%, and the samples were frozen. Samples were then thawed, spun and filtered. Samples were analyzed for monatin using the LC/MS/MS methodology described in Example 3. The results are shown in Table 22.

TABLE 22 Polypeptides monatin (ppm) monatin (ppm) monatin (ppm) (SEQ ID NO) 2 hours 8 hours 24 hours B. sphaericus 179 774 1482 912 0.2 2 2 914 4 22 62 916 0.6 2 6 918 158.8 402 496 920 5 40 96 922 0.2 2 2 924 nd nd nd 926 1.2 12 30 nd = not detected under conditions assayed

DAT nucleic acids having the sequence shown in SEQ ID NO:169, 171, 167, 173 and 175 (encoding DAT polypeptides having the sequence shown in SEQ ID NO:170, 172, 168, 174 and 176) were obtained as PCR products and were subcloned in pET30a as described in Example 4. One of ordinary skill in the art could reconstruct the genes using any number of gene assembly methods such as the one described in Example 4.

E. coli BL21 DE3 cells containing DAT nucleic acids having the sequence of SEQ ID NO:169, 171, 167, 173 and 175 in the pET30a vector were grown in 50 mL of Overnight Express (Novagen) in a 250 mL baffled flask, overnight at 30° C. and 250 rpm. The cells were collected by centrifugation when the optical density at 600 nm was greater than 10.

Cell extracts were prepared as described in the Example 4. Total soluble protein and DAT protein concentrations were determined as described in Example 4. The polypeptide having the sequence of SEQ ID NO:170 (encoded by the DAT nucleic acid having the sequence of SEQ ID NO:169) did not appear to be soluble, which may have impeded activity assays.

An R,R monatin formation assay was performed as described in Example 5 using a DAT polypeptide concentration of 0.5 mg/mL, except that 0.25 mg/mL of the SEQ ID NO:170 polypeptide was used. As a positive control, purified B. sphaericus DAT was tested at a 0.5 mg/mL concentration. After 2, 8 and 24 hours, an aliquot was taken, formic acid was added to a final concentration of 2%, and the samples were frozen. Samples were then thawed, spun and filtered, and analyzed for monatin using the LC/MS/MS methodology described in Example 3. Results are shown in Table 23.

TABLE 23 Polypeptides monatin (ppm) monatin (ppm) monatin (ppm) (SEQ ID NO) 2 hr 8 hr 24 hr B. sphaericus 456 1502 2970 170 2 8 14 172 5 20 60 168 15 68 186 174 1 4 8 176 451 1508 2744

Samples (without pH adjustment) were analyzed to determine % R,R using the FDAA derivatization protocol described in Example 3. The monatin produced by DAT polypeptide having the sequence shown in SEQ ID NO:176 was 99.6% R,R after 24 hrs compared to that produced by B. sphaericus, which was 95.2% R,R at the same timepoint. The activity and stereopurity resulting from the DAT polypeptide having the sequence of SEQ ID NO:176 were both quite high, and the corresponding nucleic acid was subcloned as a C-terminal tagged protein as described in Example 4 for more quantitative studies.

Characterization of SEQ ID NO:176 C-His-Tagged Protein

The nucleic acid having SEQ ID NO:175, which encodes the polypeptide having the sequence of SEQ ID NO:176, was cloned into pET30a without a stop codon so that it could be expressed as a fusion protein with a 6×His-tag on the C-terminus. The protein was purified using the His-bind resin described in Example 4. When the fusion protein was eluted from the PD-10 desalting column, a yellow precipitate formed in the solution. A yellow residue was also observed on the column. The yellow color usually is indicative of the presence of a PLP-binding protein. In an effort to prevent the precipitation of the PLP-binding protein at the desalting step, different buffers (100 mM phosphate and 100 mM EPPS with or without 10% glycerol) at two pH values (7.8 and 8.2) were utilized. None of the buffers tried appeared to completely prevent the precipitation.

The monatin assay was performed using a well-mixed heterogeneous protein solution and a DAT polypeptide concentration of 0.5 mg/mL. The results are shown in Table 24. The purified SEQ ID NO:176 DAT polypeptide (C-tagged) showed comparable activity to the positive control DAT polypeptide from B. sphaericus; however, the activity appeared to be lower than the activity exhibited by the mutant polypeptides having the sequence of SEQ ID NO:870 T242N or SEQ ID NO:870 T242N.

TABLE 24 Monatin Production (ppm) Polypeptide (SEQ ID NO) 2 hr 4 hr 8 hr 24 hr B. sphaericus 262 676 1044 2150 870 332 678 1346 2826 870 T242N 942 1938 2834 4004 176 208 392 732 1806

Example 14 Evaluation of DATs

The open reading frames encoding 29 DATs were obtained as PCR products. It is noted that one of ordinary skill in the art can synthesize the genes encoding the DATs using assembly techniques such as those described in Example 4. The DAT nucleic acids were subcloned into the pET30a vector and expressed as untagged proteins as described in Example 4. The desalted cell-free extracts (prepared as described in Example 4) were used in monatin formation assays. A DAT polypeptide concentration of 0.5 mg/mL was used for the monatin assay except for the following polypeptides (amounts used in parentheses): the SEQ ID NO:156 polypeptide (0.4 mg/mL), the SEQ ID NO:182 polypeptide (0.45 mg/mL), the SEQ ID NO:240 polypeptide (0.47 mg/mL), and the SEQ ID NO:204 polypeptide (0.42 mg/mL).

Most of the DAT polypeptides showed undetectable to low monatin production under the conditions assayed as compared to positive control enzymes. Most of the expressed DAT polypeptides were soluble as determined by the Experion; however, the polypeptides having SEQ ID NO:204 and 240 were expressed at very low levels and may not have been very soluble. On the other hand, the polypeptide having SEQ ID NO:220 was predicted to be 68% of the total soluble protein as judged by the Experion software.

The monatin production results are shown in Table 25 and 26. At 24 h, the DAT polypeptide having SEQ ID NO:156 and 214 produced 40-50% of monatin as compared to the positive control enzyme, the DAT from B. sphaericus. The most active DAT polypeptide was the SEQ ID NO:220 polypeptide. Approximately 4 h after the reaction was started, the monatin concentration reached a maximum. It is expected that the mature protein of SEQ ID NO:156 (without the predicted leader sequence) is the active component of the DAT polypeptide and, therefore, the protein can be produced recombinantly with the leader sequence absent.

TABLE 25 Monatin Formed (ppm) Polypeptide (SEQ ID NO) 2 hr 4 hr 8 hr 24 hr 178 11 24 62 194 180 nd nd nd nd 154 52 103 166 178 182 nd nd nd nd 218 1.6 2.8 274 12 188 2.4 5.2 10 22 190 3.8 9.4 22 42 208 1 1.2 nd nd 220 2418 3563 3812 3882 196 1 1.8 nd 8 156 64.8 156 296 796 B. sphaericus 422 791 1302 2124 nd = not detectable under conditions assayed

TABLE 26 Monatin Formed (ppm) Polypeptide (SEQ ID NO) 2 4 8 24 166 nd nd 1 nd 216 69 91 109 134 200 nd nd 1 1 198 nd nd nd nd 210 1.6 3.8 6.2 15.2 202 3.4 6.2 12.2 29.8 222 3.6 7 12.8 25.8 236 nd nd nd nd 204 nd nd nd 3.6 238 10.4 21.8 46.4 115.6 240 3 6 12.4 32.2 224 39.8 85 171.8 268.8 226 2.6 5.8 12.4 30.6 228 4.2 9.8 21.4 66.8 230 9.2 21.8 42.2 94.4 232 3.6 9.4 21.6 57 246 nd nd nd nd 214 160 327 694 1346 B. sphaericus 393 986 1624 2597 nd = not detectable under conditions assayed

The high activity of the SEQ ID NO:220 polypeptide was confirmed in another monatin formation assay where the SEQ ID NO:220 polypeptide was compared to the SEQ ID NO:870, 870 T242N, and B. sphaericus DAT polypeptides. A DAT polypeptide concentration of 0.1 mg/mL and 0.5 mg was used for each of the DAT polypeptides assayed. The results are shown in Table 27. Due to the high degree of activity of the DAT polypeptides assayed, the monatin samples had to be diluted 100-fold to be within the quantitative range of the instruments used (as opposed to a typical 10- or 20-fold dilution).

TABLE 27 Monatin Formed (ppm) DAT Polypeptide (SEQ ID NO) 2 hr 4 hr 8 hr 24 hr B. sphaericus (0.1 mg/mL) 74 170 309 728 B. sphaericus (0.5 mg/mL) 510 921 1068 2704 870 (0.1 mg/mL) 28 81 179 706 870 (0.5 mg/mL) 399 847 1466 2916 870 T242N (0.1 mg/mL) 93.2 245.8 582.4 1270 870 T242N (0.5 mg/mL) 1158.8 2026 3202 4126 220 (0.1 mg/mL) 965.8 1512 2330 3788 220 (0.5 mg/mL) 2950 4302 4618 4488

The percentage of R,R formed by the DAT polypeptide having SEQ ID NO:220 in the above experiments was determined using the FDAA derivatization methodology described in Example 3. The DAT polypeptide having the sequence of SEQ ID NO:220 is highly stereospecific, producing 99.3% R,R monatin at 24 hours as compared to 92.9% R,R for B. sphaericus. In another assay, the SEQ ID NO:220 polypeptide produced 99.8% R,R-monatin.

The DAT polypeptide having the sequence of SEQ ID NO:220 is a novel protein that is 62% identical at the protein level to the C. beijerinckii DAT polypeptide shown in Example 9. The SEQ ID NO:220 polypeptide has 86%-90% primary sequence homology to other highly active DAT polypeptides (e.g., those having the sequence shown in SEQ ID NO:892 and 894 (Example 8), 946 (Example 7) and 176 (Example 13)). These highly active and novel DAT polypeptides were uncharacterized prior to this work, and these enzymes exhibited higher activity and stereospecificity for R,R monatin production reactions than any of the published Bacillus-like D-aminotransferases. FIG. 3 shows an alignment of these related D-aminotransferases and the consensus sequence motifs they have in common are described below.

Consensus Sequence C (SEQ ID NO: 1071) M.*GYYNG.*P.*DR.*FGDG.*YDAT.*N.*FAL.*H.*RF.*NS.*LL.*I.*K.*YWQ.*RG.*G.*R.* H.*F.*N.*I.*P.*KLI.*DTRF.*HCNIKTLNL.*P.*VIA.*Q.*E.*C.*E.*VFHRG.*VTECAHSN.*I. *NLIL.*G.*HL.*P.*E.*F.*L.*ADE.*V.*SS.*DG.*GGK.*K.*Q.*T Consensus Sequence D (SEQ ID NO: 1072) M.{3}GYYNG.{10}P.{2}DR.{3}FGDG.YDAT.{3}N.{3}FAL.{2}H.{2}RF.NS.{2}LL.I.{9}K. {17}YWQ.{2}RG.G.R.H.F.{5,7}N.{2}I.{3}P.{10}KLI.{3}DTRF.HCNIKTLNL.P.VIA.Q.{3}E. {2}C.E.VFHRG.{2}VTECAHSN.{2}I.{11}DNLIL.G.{4}HL.{9}P.{2}E.{2}F.{4}L.{2}ADE. {2}V.SS.{10}DG.{3}GGK.{5}K.{2}Q.{10}T Consensus Sequence E (SEQ ID NO: 1073) M.*[LV]GYYNG.*[LI].*[ML].*[VI]P.*DR.*[YF]FGDG.*YDAT.*N.*FAL[DE][ED]H[IL][DE] RF.*NS.*LL*I.*[KR].*[EQ][LMV]K[KE].*[MV].*[DE].*[VL]YWQ.*[TS]RG[TS]G.*R[NS] H.*F.*N[LI].*I.*P.*[IVL].*[KE].*KLI[TS].*[ED]DTRF.*HCNIKTLNL[IL]P[NS]VIA[SA]Q[R K].*E.*C.*E.*VFHRG[ED].*VTECAHSN[V1].*I[IL][KR][ND].*[TS].*DNLIL.*G.*HL[LI][Q K].*[IV]P.*E.*F[TS][LM].*[ED]L.*ADE[VI][LI]V[ST]SS.*[LM1].*[IL]DG.*GGK.[LVI]K.* [IL]Q.*[EK][FY].*T

Similar to PERL regular expression convention language (perldoc.perl.org), “.*” indicates that any number of amino acid residues may be present from any of the 20 proteinogenic amino acids; [ ] indicates that any one of the amino acids in the brackets can be present; “.{#}” indicates that any of the 20 proteinogenic amino acids can be present as long as the number of residues matches the number (#) indicated in the brackets.

With respect to the use of “.*” in Consensus sequence C, the number of amino acids at any of the “.*” positions can vary, for example, from about 0 to about 20 residues (see, for example, Consensus sequences D (SEQ ID NO:1072) and E (SEQ ID NO:1073)) or from about 20 residues up to about 100 residues, or the number of amino acids can be much larger, for example, up to 1000 or more residues. Without limitation, an insertion at one or more of the “.*” positions can correspond to, for example, a domain such as (but not limited to) a chitinase binding domain (e.g., from Pyrococcus litriosus (Accession No. 2CZN_A) or P. burkholderia (Accession No. YP_(—)331531) or a cellulose binding domain (e.g., from Cellulomonas fimi (Accession No. 1EXH_A) or Clostridium stercorarium (Accession No. 1UY1_A). In some embodiments (without limitation), five or less of the positions designated “.*” each contain an insertion of, for example, greater than about 20 residues (e.g., greater than about 100 residues). In other embodiments (without limitation), five or more of the positions designated “.*” each contains an insertion of less than about 100 residues (e.g., less than about 20 residues, e.g., 3, 5, 10, 20, 25, 30, 40, 50, 60, 70, 75, 80, 90, or 95 residues). The activity of a polypeptide having a sequence that corresponds to one or more of the consensus sequences disclosed herein and containing any number of residues inserted at one or more of the “.*” positions can be evaluated using methods that are described herein.

Non-limiting representative polypeptide that contain the consensus sequence shown in SEQ ID NO:1071 include SEQ ID NO:220, 892, 894, 176 and 946 and Consensus sequences D (SEQ ID NO:1072) and E (SEQ ID NO:1073)). It is expected that any D-aminotransferase exhibiting any of consensus sequences C, D, or E would be active in monatin formation pathway steps.

Characterization of a c-His-Tagged Polypeptide Having the Sequence of SEQ ID NO:220

The DAT nucleic acid having the sequence of SEQ ID NO:219 (encoding the polypeptide of SEQ ID NO:220) was cloned into pET30a without a stop codon such that it was expressed as a fusion protein with a 6×His-tag on the C-terminus (as described in Example 4). The fusion protein was purified using the His-Bind column (Novagen) described in Example 4. The eluate from the PD-10 desalting column formed a yellow precipitate. Yellow residue was also observed on the column. Monatin assays were done using a well-mixed heterogenous protein solution. Amounts of DAT polypeptide used are indicated in parentheses in the far left column. The notation w/Trp indicates that the enzyme was incubated with 10 mM D-tryptophan overnight on ice. Results are shown in Table 28.

TABLE 28 Monatin formed (ppm) Polypeptide (SEQ ID NO) 2 hr 4 hr 8 hr 24 hr B. sphaericus (0.5 mg/mL) 382 660 1021 1986 870 T242N (0.1 mg/mL) 69 205 412 1074 870 T242N w/Trp (0.1 mg/mL) 63 163 383 978 870 T242N (0.5 mg/mL) 919 1698 2356 3130 870 T242N w/ Trp (0.5 mg/mL) 772 1519 2294 3023 220 (0.1 mg/mL) 847 1462 2202 3004 220 (0.1 mg/mL) 811 1522 2202 2887 220 w/ Trp (0.1 mg/mL) 537 1080 1590 2401 220 (0.5 mg/mL) 2885 3446 3813 4066 220 w/ Trp (0.5 mg/mL) 1933 3223 3939 3911

The reaction containing 0.1 mg/mL of the SEQ ID NO:220 polypeptide showed a similar monatin formation time course as the reaction containing 0.5 mg/mL of the SEQ ID NO:870 T242N polypeptide. Addition of D-tryptophan (10 mM) to the solution containing the purified protein eliminated the precipitation. Activity loss was observed for the sample in which SEQ ID NO:220 was incubated with D-tryptophan (10 mM) overnight on ice, but no negative effect was observed when the SEQ ID NO:870 T242N polypeptide was treated with D-tryptophan (10 mM). The presence of HMG was also analyzed qualitatively for reactions catalyzed by the SEQ ID NO:220 polypeptide by comparing peak areas. When both DAT polypeptides were utilized at a concentration of 0.5 mg/mL, the reaction catalyzed by the SEQ ID NO:220 polypeptide formed around 40% of the HMG compared to the reaction containing the SEQ ID NO:870 T242N polypeptide. Earlier time points show an even more pronounced difference between the two enzymes.

In an attempt to prevent the protein precipitation during the purification of the SEQ ID NO:220 polypeptide, DTT (5 mM) was included in all the buffers including the Bugbuster reagent, the buffers for His-Bind column and the buffer for PD-10 column. No precipitation was observed after the desalting column, and no negative effect was observed on the activity of the SEQ ID NO:220 polypeptide when DTT was included during purification or added into purified protein solution at a concentration of 2 mM. Data for monatin formation assays are shown in Table 29. The amount of DAT polypeptide used is indicated in the left column in parentheses. “added DTT” indicates that 2 mM DTT was added to resolubilize the protein after purification; and “purified w/DTT” indicates that 5 mM DTT was present throughout the purification.

TABLE 29 Monatin Formed (ppm) Polypeptide (SEQ ID NO) 2 hr 4 hr 24 hr B. sphaericus 426 965 2638 (0.5 mg/mL) 870 T242N (0.5 mg/mL) 977 1916 4227 220 (0.1 mg/mL) 1214 2163 3964 220 (0.5 mg/mL) 3534 4246 4415 220 added DTT (0.1 mg/mL) 1287 2202 3566 220 added DTT (0.4 mg/mL) 3495 4833 5082 220 purified w/ DTT (0.1 mg/mL) 1204 2169 3997 220 purified w/ DTT (0.5 mg/mL) 3562 4110 4353

The highly desirable properties of the SEQ ID NO:220 polypeptide make it an excellent candidate for further mutagenesis or directed evolution experiments.

Site-Directed Mutagenesis of the SEQ ID NO:220 Polypeptide

A loop region of the DAT polypeptide related to the Bacillus DAT polypeptide was identified as being important for the substrate specificity and stereospecificity of the enzymes (Ro et al., 1996, FEBS Lett, 398:141-145; Sugio et al., 1995, Biochemistry 34:9661-9669; and EP 1 580 268). One key residue in this region is a T at residue 242 (in the DAT polypeptide from ATCC #4978, this position corresponds to a Tat residue 243). A T242N mutant of the SEQ ID NO:870 polypeptide showed improvement in both activity and stereospecificity, as did the T243N mutant of DAT 4978 (see Example 10). Primary sequence alignment of the SEQ ID NO:220 polypeptide with the SEQ ID NO:870 polypeptide showed only 35% amino acid sequence identity and 65% homology. The T242 residue in SEQ ID NO:870 aligned with a G240 residue in SEQ ID NO:220, which is followed by a T241 residue. Using Accelrys DS Modeler software for both proteins (with Bacillus YM-1 structures as templates), it was difficult to overlap the loop region of the SEQ ID NO:870 polypeptide with the SEQ ID NO:220 polypeptide. Therefore, both amino acids were chosen as targets for site-directed mutagenesis.

A mutant polypeptide designated SEQ ID NO:220 G240N and SEQ ID NO:220 T241N were generated by site-directed mutagenesis of the corresponding nucleic acid (SEQ ID NO:219) as described in Example 4. The two mutant polypeptides were expressed and purified as 6×His-tagged fusion proteins that were used in the monatin formation assay. Yellow precipitation was observed at the desalting step for both of the mutant SEQ ID NO:220 polypeptides. Results are shown in Tables 30 and 31 for monatin formation assays. The amount of D-aminotransferase used is indicated in the left-hand column in parentheses. Different preparations of the SEQ ID NO:220 polypeptide were utilized in the assay. “ferm” indicates that the SEQ ID NO:220 polypeptide used was produced in a fermentor as described in Example 15.

TABLE 30 Monatin Produced (ppm) Polypeptide (SEQ ID NO) 2 hrs 4 hrs 8 hrs 24 hrs B. sphaericus (0.5 mg/mL) 440 777 1510 2621 870 T242N (0.5 mg/mL) 961 1913 2793 3904 ferm 220 (0.1 mg/mL) 1396 2379 3217 3770 ferm 220 (0.2 mg/mL) 2301 3277 3789 4328 ferm 220 w/ DTT (0.1 mg/mL) 1434 2384 3109 3730 ferm 220 w/ DTT (0.2 mg/mL) 2423 3568 3859 4755 220 (0.1 mg/mL) 1109 1912 2809 3713 220 T241N (0.1 mg/mL) 554 856 1084 1986

TABLE 31 Monatin Formed (ppm) Polypeptide (SEQ ID NO) 2 hr 4 hr 8 hr 24 hr B. sphaericus (0.5 mg/mL) 634 938 1651 2754 870 T242N (0.5 mg/mL) 1422 1922 3211 3793 ferm 220 (0.1 mg/mL) 1976 2505 3442 4211 ferm 220 (0.2 mg/mL) 3198 3430 4452 4639 220 G240N (0.1 mg/mL) 3 5 14 42 220 G240N (0.2 mg/mL) 9 17 46 94

A very small amount of monatin (95.7% R,R monatin) was formed in the reaction catalyzed by the mutant SEQ ID NO:220 G240N polypeptide. The mutant SEQ ID NO:220 T241N polypeptide lost about 50% of the activity, but still maintained the stereospecificity (99.7% R,R monatin produced). These results, together with the homology modeling and alignments, suggest that, in the region surrounding residues 242-243 (and potentially beyond), the structure of the SEQ ID NO:220 polypeptide is not similar to the structure of the SEQ ID NO:870 polypeptide or the structure of the Bacillus-like DAT polypeptide in the literature. Since there is no x-ray crystal structure, random mutagenesis, combinatorial approaches and other directed evolution approaches of the SEQ ID NO:220 polypeptide and related DAT polypeptides are expected to be highly productive in further improving the enzyme's activity.

Example 15 Production of a DAT in a Fermentor

Bacterial growth media components were from Difco, Fisher Scientific, or VWR; other reagents were of analytical grade or the highest grade commercially available. The fermentation was run in a New Brunswick Scientific (Edison, N.J.) BioFlo 3000® fermenter. Centrifugation was carried out using a Beckman (Fullerton, Calif.) Avanti® J-25I centrifuge with a JLA-16.250 or JA-25.50 rotor.

The DAT nucleic acid encoding the polypeptide having the sequence in SEQ ID NO:220 with a C-terminal His-tag was cloned using Nde I/Xho I restriction sites into the pMet1a vector described in Example 16. The antibiotic marker (bla gene) can further be removed using Psi I restriction enzyme digestion, gel purification of the vector band, self-ligation of the vector ends, transformation into the E. coli host, and selection on minimal medium plates that do not contain methionine. Typically, Neidhardt's medium with 15 amino acids is used. The cloning sites were NdeI/XhoI to insert the SEQ ID NO:220 nucleic acid sequence into pMET1a (see Example 16).

The SEQ ID NO:220 DAT polypeptide carrying a C-terminal His-purification tag was produced in a fermentor at the 2.5-L scale, in a fed-batch process that achieves high cell densities and high levels of expression of the desired protein. The protocol and results for growth of E. coli strain B834(DE3)::SEQ ID NO:220cHIS pMET1 are described as follows: Starting from a fresh culture plate (Neidhardt's+15 amino acids, no methionine), the cells were grown in 5 mL of Neidhardt's medium supplemented with 15 amino acids, at 30° C. and 225 rpm for 6-8 h. One mL of the culture was transferred to each of 2 125-mL aliquots of the production medium supplemented with 5 g/L of glucose. The flasks were grown at 30° C. and 225 rpm overnight (16-18 h). A fermentor was charged with 2.5 liters of the production medium, containing (per liter): 2.0 g/L (NH₄)₂SO₄; 8.0 g/L K₂HPO₄; 2.0 g/L NaCl; 1.0 g/L Na₃Citrate.2H₂O; 1.0 g/L MgSO₄. 7H₂O; 0.025 g/L CaCl₂.2H₂O; 0.05 g/L FeSO₄.7H₂O; 0.4 mL/L Neidhardt micronutrients, and 2.0 g/L glucose. The fermenter was inoculated with 10% v/v of the overnight culture. Three hours after inoculation, an exponential glucose feed was set up using a 60% w/v glucose solution. The feed was supplied at the required rate to support microbial growth at an exponential rate of 0.15 h⁻¹. When the carbon dioxide evolution rate (CER) had reached a value of 100 mmoles/L/h (approximately 21 hours after inoculation; corresponding to a cell biomass of 15-16 g DCW/L), gene expression was induced with a bolus addition of 2 g/L lactose (fed as a 20% solution). The feed was changed from 60% w/v glucose to 50% w/v glucose+10% w/v lactose while the feed rate was fixed to the rate at time of induction. The “50% w/v glucose+10% w/v lactose” feed was maintained for 6 hours. At the end of the fermentation, the cells were harvested by centrifugation at 5000-7000×g for 10 min and frozen as a wet cell paste at −80° C. Cell paste (318 grams) was harvested from 2.8 L of cell broth.

To prepare cell free extract containing the SEQ ID NO:220 polypeptide, 50 g of wet cell paste was suspended in 150 mL of 50 mM EPPS buffer (pH 8.4) containing 50 μM pyridoxal phosphate (PLP) and then disrupted using a Microfluidics homogenizer (Microfluidics, Newton, Mass.) (3 passes at 18,000 psi), maintaining the temperature of the suspension at less than 15° C. The cell debris was removed by centrifugation (20,000×g for 30 minutes). Two mM DTT was added to the clarified cell extract.

To prepare purified SEQ ID NO:220, 2×25 mL aliquots of clarified cell extract were loaded each onto a 45 mL Chelating Sepharose™ Fast Flow resin (nickel(II) form) column that had been previously equilibrated with 50 mM EPPS (pH 8.4) containing 0.05 mM PLP and 200 mM sodium chloride. After loading the sample, the column was washed/eluted successively with 3-5 volumes of the equilibration buffer, 3-5 volumes of the equilibration buffer containing 25 mM imidazole, 3-5 volumes of the equilibration buffer containing 100 mM imidazole and 3-5 volumes of the equilibration buffer containing 500 mM imidazole. The 500 mM imidazole eluent was concentrated 10× with an Amicon (Billerica, Mass.) Centricon-70 centrifugal filter device (MWCO 10 kDa). The imidazole and sodium chloride were removed by passage through disposable GE Healthcare PD10 desalting columns previously equilibrated with 50 mM EPPS (pH 8.4) containing 0.05 mM PLP. The protein concentration of the desalted solution was determined using the Pierce BCA assay kit (Rockford, Ill.). The purity of each fraction and the level of expression in the cell free extract fraction were determined by SDS-PAGE with 4-15% gradient gels. Approximately 450 mg of protein that was ˜90% pure was recovered from the 50 mL of clarified cell extract. Two mM DTT was added to 10 mL of the purified protein. The purified protein was dispensed into aliquots (0.5-5 mL) and stored at −80° C.

Bench scale reactions (250 mL) were carried out in 0.7 L Sixfors agitated fermenters (Infors AG, Bottmingen, Switzerland) under a nitrogen headspace. The reaction mix contained 10 mM potassium phosphate, 1 mM MgCl₂, 0.05 mM PLP, 200 mM sodium pyruvate and 100 mM D-tryptophan. The reaction mix was adjusted to the appropriate temperature, and adjusted to the appropriate pH with potassium hydroxide. The aldolase described in Example 6 was added as a clarified cell extract at 0.02 mg/mL of target protein. The SEQ ID NO:220 DAT polypeptide was added (either as purified enzyme or as a clarified cell extract) at 0.25 mg/mL of target protein.

The progress of the reactions was followed by measuring monatin concentration using the LC/MS/MS methodology described in Example 3.

Starting with D-tryptophan and under the conditions tested, the pH optimum of the monatin formation reactions using the SEQ ID NO:220 polypeptide was found to be approximately pH 8.0 and the temperature optimum of the monatin formation reactions utilizing the SEQ ID NO:220 polypeptide was found to be approximately 25° C. These reactions have complex dynamics and the optimum reaction conditions for the full monatin production reaction may not be the same as the optimal conditions for individual reactions catalyzed by the DAT polypeptide.

Example 16 The Co-Expression of Chaperones to Increase the Soluble Expression of a DAT Polypeptide

Because the soluble expression of the SEQ ID NO:894 DAT polypeptide was low using the standard expression protocols (either 1 mM IPTG in LB or Novagen Overnight Express Autoinduction System2, see Example 8), co-expression of the SEQ ID NO:894 polypeptide and a variety of commercially available chaperones was examined.

Chaperones:

The TaKaRa Chaperone Set (TAKARA BIO catalog #3340) consists of five different sets of chaperones developed by HSP Research Institute, Inc. They are designed to enable efficient expression of multiple molecular chaperones known to work in cooperation in the folding process. The set contained the following:

Resistance Plasmid Chaperone Promoter Inducer Marker pG- dnaK-dnaJ-grpE; araB; L-Arabinose; chloramphenicol KJE8 groES-groEL Pzt1 tetracycline pGro7 groES-groEL araB L-Arabinose chloramphenicol pKJE7 dnaK-dnaJ-grpE araB L-Arabinose chloramphenicol pG-Tf2 groES-groEL-tig Pzt1 Tetracycline chloramphenicol pTf16 tig araB L-Arabinose chloramphenicol Transformation Protocol

Chemically competent BL21 (DE3) cells (EMD Biosciences/Novagen catalog #69450) were transformed with 20 ng of one of the TaKaRa chaperone plasmids and 20 ng of SEQ ID NO:893/pET30a (encoding the SEQ ID NO:894 polypeptide; see Example C2 for the plasmid construction) by heat shock for 30 seconds at 42° C. The transformed cells were recovered in 0.5 mL of SOC medium for 1 hr at 37° C. and plated on LB plates containing 50 mg/L kanamycin and 25 mg/L chloramphenicol. The plates were incubated overnight at 37° C. Colonies were picked from the overnight plates and used to inoculate 5 mL of 2×YT medium containing 50 mg/L kanamycin and 25 mg/L chloramphenicol. After overnight incubation at 37° C., the plasmids were isolated from the cell pellets using a QUIAprep Spin Miniprep Kit (Qiagen catalog #27104). The plasmids were analyzed by restriction digestion with one-cutter enzymes from New England Biolabs (Beverly, Mass.) for both the chaperone plasmid and the SEQ ID NO:893/pET30a plasmid following the manufacture's recommended protocol.

Plasmid Restriction Enzyme pG-KJE8 XhoI pGro7 XbaI pKJE7 NheI pG-Tf2 XhoI pTf16 XbaI

The isolated DNA containing both SEQ ID NO:893/pET30a and pKJE7 was digested with NheI and XbaI.

Expression Studies

Flasks of Novagen Overnight Express™ AutoinductionSystem 2 (EMD Biosciences/Novagen catalog #71366) containing solutions 1-6, 50 mg/L kanamycin and 25 mg/L chloramphenicol (25 mL in each flask) were inoculated from fresh plates of the cells co-transformed with a chaperone plasmid and SEQ ID NO:893/pET30. At inoculation the inducers required for the chaperone plasmids were also added.

Plasmid Inducer concentration pG-KJE8  2 mg/mL L-arabinose; 10 ng/mL tetracycline pGro7  2 mg/mL L-arabinose pKJE7  2 mg/mL L-arabinose pG-Tf2 10 ng/mL tetracycline pTf16  2 mg/mL L-arabinose

The cells were incubated at 30° C. overnight and harvested by centrifugation when the OD at 600 nm reached 6 or greater. The cells were washed with cold 50 mM EPPS buffer (pH 8.4), centrifuged again, and either used immediately or frozen at −80° C.

Cell extracts were prepared by adding 5 mL per g of cell pellet of BugBuster® (primary amine-free) Extraction Reagent (EMD Biosciences/Novagen catalog #70923) with 5 μL/mL of Protease Inhibitor Cocktail II (EMD Bioscience/Calbiochem catalog #539132), 1 μl/mL of Benzonase® Nuclease (EMD Biosciences/Novagen catalog #70746), and 0.033 μl/mL of r-Lysozyme solution (EMD Biosciences/Novagen catalog #71110) to the cells. The cell suspensions were incubated at room temperature with gentle mixing for 15 min; spun at 14,000 rpm for 20 min (4° C.) and the supernatants carefully removed. Total protein concentrations were determined using the Pierce BCA protein assay kit (Pierce catalog #23225) with bovine serum albumin as the standard and a microtiter plate format. The expression of the D-aminotransferase was analyzed by SDS-PAGE using Bio-Rad Ready Gel® Precast 4-15% polyacrylamide gradient gels (Bio-Rad Laboratories catalog #161-1104). BioRad SDS-PAGE low range standards (catalog #161-0304) were run as standards on each gel. Aliquots of the cell extracts (15 μg protein) were mixed with protein loading buffer containing 2% SDS, 10% glycerol, 12.5% 2-mercaptoethanol, 0.1% bromophenol blue and 62.5 mM Tris-HCl (pH 8), incubated at 95° C. for 5 min, cooled and then loaded on the gel. In addition, the combined soluble and insoluble protein expression (total protein) was analyzed for each transformant. A 10 μl aliquot of each cell suspension before centrifugation was diluted in 90 μL protein loading buffer, incubated at 95° C. for 10 min, and cooled. Ten μl of each cooled solution was loaded on the gel.

The soluble protein gel showed that the best soluble expression of the polypeptide having the sequence of SEQ ID NO:894 occurred when chaperones GroEL-GroES (pGro7) were co-expressed.

The expression of the SEQ ID NO:894 polypeptide using an alternative plasmid in the presence of the GroEL-GroES chaperones was also examined. The SEQ ID NO:893 nucleic acid was subcloned into the pMET1a plasmid using the restriction enzymes NdeI and XhoI from New England Biolabs. This plasmid is a derivative of pET23a (EMD Biosciences/Novagen catalog #69745-3) and carries the metE gene (inserted at the NgoMIV site of the plasmid) and can complement the methionine auxotrophy of E. coli strains B834(DE3) and E. coli BW30384 (DE3) ompTmetE (“EE2D”). The construction of the “EE2D” strain is described in WO 2006/066072. The construction of an analogous plasmid to pMET1a that is a derivative of pET23d is described in the same PCT application.

The SEQ ID NO:893/pMET1a plasmid (25 ng) was transformed into “EE2D” electrocompetent cells singly or was co-transformed with pGro7 (20 ng) using the standard Bio-Rad electroporation protocol for E. coli cells with a Bio-Rad Gene Pulser II system (catalog #165-2111). The transformed cells were recovered in 0.5 mL of SOC medium for 1 h at 37° C. and plated on LB plates containing 100 mg/L ampicillin or on plates containing 100 mg/L ampicillin and 25 mg/L chloramphenicol (double plasmid transformants). The plates were incubated overnight at 37° C. One colony from each plate set was used to inoculate 50 mL of Novagen Overnight Express™ AutoinductionSystem 2 containing solutions 1-6, 100 mg/L ampicillin and 2 mg/mL L-arabinose. The culture inoculated with cells containing the pGro7 plasmid also contained 25 mg/L chloramphenicol. The cells were incubated at 30° C. overnight and harvested by centrifugation when the OD₆₀₀ reached 6 or greater. The cell pellets were washed with cold 50 mM EPPS buffer (pH 8.4), centrifuged again, and either used immediately or frozen at −80° C. Cell extracts were prepared as described above using the Novagen BugBuster® (primary amine-free) Extraction Reagent. The expression of soluble and total D-aminotransferase was analyzed by SDS-PAGE as described above.

The gel showed that expression of soluble SEQ ID NO:894 polypeptide was greater when the GroEL-GroES proteins were co-expressed. However, the soluble expression was not as high as with the pET30a construct described above.

The effect of incubation temperature during expression was also examined. A 5 mL culture of LB containing 100 mg/L ampicillin and 25 mg/L chloramphenicol was inoculated from a fresh plate of EE2D::SEQ ID NO:894PMET1a+pGro7. The culture was incubated overnight at 30° C. and then used to inoculate 3 flasks, each containing 50 mL of Novagen Overnight Express™ Autoinduction System 2 containing solutions 1-6, 100 mg/mL ampicillin, 25 mg/L chloramphenicol, and 2 mg/mL L-arabinose. One flask was incubated at 20° C., the second at 25° C. and the third at 30° C. The cells were harvested when the OD₆₀₀ reached 6 or greater. The cells were harvested and cell extracts were prepared as described above. Total protein concentrations were determined using the Pierce BCA protein assay kit (Pierce catalog #23225) with bovine serum albumin as the standard and a microtiter plate format. The expression of the D-aminotransferase was analyzed using the Bio-Rad Experion Pro260 Automated Electrophoresis Station following the manufacturer's protocol with the cell extract solutions diluted to 1 mg/mL. The results are shown in Table 32. It appears that the lowest temperature gave the maximum amount of expression of the SEQ ID NO:894 polypeptide.

TABLE 32 Estimated DAT Lane Sample Temp Expression 1 Pro260 Ladder 2 EE2D::23463pMET1 + pGRO7 20° C. 23% cell extract 3 EE2D::23463pMET1 + pGRO7 25° C. 21% cell extract 4 EE2D::23463pMET1 + pGRO7 30° C. 19% cell extract Activity Assay Protocol

The enzymatic activity of the SEQ ID NO:894 DAT co-expressed with the GroEL-GroES chaperones was tested following the standard monatin reaction protocol. Briefly, each assay tube contained the following (in a total of 2 mL): 0.050 mg/mL aldolase in cell extract (assuming 20% soluble expression); 1.0 mg/mL D-aminotransferase in cell extract (assuming 20% soluble expression for an extract containing the SEQ ID NO:894 polypeptide) or purified B. sphaericus D-aminotransferase; 0.01% Tween-80; 200 mM sodium pyruvate; 100 mM D-tryptophan; 100 mM EPPS (pH 8.2); 1 mM MgCl₂; 0.05 mM PLP; and 10 mM potassium phosphate.

The reactions were incubated at room temperature in a Coy Laboratory Products, Inc. anaerobic chamber to minimize exposure to oxygen. All components except the enzymes were mixed together (the tryptophan did not completely dissolve until at least 1 h after the addition of the enzymes). The reactions were initiated by the addition of the enzymes. Samples were withdrawn at 1, 4, 8 and 22 h. A control reaction using 1 mg/mL purified B. sphaericus DAT was also run. The construction, expression and purification of this DAT are described in Example 6. The concentrations of the substrates and products were measured as described in Example 3.

The results are shown in Table 33. At 22 h, the concentration of monatin was 9.2 mM when the SEQ ID NO:894 polypeptide was present and 12.4 mM when the B. sphaericus enzyme was used. The concentration of the co-product HMG was significantly less when the SEQ ID NO:894 polypeptide was in the assay mixture (<⅓ the concentration when compared to the assay sample containing B. sphaericus enzyme). The HMG concentrations were evaluated by comparing the peak areas of the OPA derivatized samples.

TABLE 33 Monatin Formation (mM) Polypeptide (SEQ ID NO) 1 h 4 h 8 h 22 h 894 DAT + GroEL-ES 1.3 4.5 6.8 9.2 B. sphaericus DAT 1.6 5.7 8.3 12.4

Example 17 Use of ArcticExpress™ System to Increase the Soluble Expression of a DAT Polypeptide

Because the soluble expression of the SEQ ID NO:894 polypeptide was low using the standard expression protocols (either 1 mM IPTG in LB or Novagen Overnight Express™ AutoinductionSystem 2—see Example 8), expression of the SEQ ID NO:893/pMET1a plasmid in the Stratagene ArcticExpress™ system was examined.

The Stratagene ArcticExpress™ system contains E. coli competent cells that carry the psychrophilic chaperones Cpn10 and Cpn60. These are chaperones isolated from the psychrophilic organism Oleispira antarctica. Cpn10 and Cpn60 show high sequence similarity to the E. coli chaperones GroEL and GroES, respectively, and have high protein folding activities at 4-12° C. The ArcticExpress™ host cells are derived from the E. coli BL21 strain. Not only do these cells lack the Lon protease, but they have been engineered to be deficient in OmpT protease as well.

Transformation Protocol

The plasmid SEQ ID NO:893/pMET1a (described in Example 16) was transformed into chemically competent ArcticExpress™ (DE3) cells (catalog #230192) following the manufacturer's protocol. The transformed cells were recovered in 0.5 mL of SOC medium for 1 h at 37° C. and plated on LB plates containing 100 mg/L ampicillin. The plates were incubated overnight at 37° C. and then stored at 4° C.

Expression Protocol

Colonies from the transformation plates were used to inoculate 5 mL of 2×YT medium containing 100 mg/L ampicillin and 10 mg/L gentamycin and incubated overnight at 30° C. Flasks of Novagen Overnight Express™ AutoinductionSystem 2 (EMD Biosciences/Novagen catalog #71366) containing solutions 1-6, with 100 mg/L ampicillin and 12 mg/L gentamycin were inoculated using the overnight cultures. After incubation at 30° C. for 6 h and the Overnight Express™ cultures were moved to either 15° C. or 20° C. or 25° C. incubators. The incubations were continued until the OD at 600 nm of the cultures reached 6 or greater. The cells were harvested by centrifugation, washed with cold 50 mM EPPS, pH 8.4, and the cell pellets were frozen at −80° C.

Cell extracts were prepared by adding 5 mL per g of cell pellet of BugBuster® (primary amine-free) Extraction Reagent (EMD Biosciences/Novagen catalog #70923) with 5 μL/mL of Protease Inhibitor Cocktail II (EMD Bioscience/Calbiochem catalog #539132), 1 μl/mL of Benzonase Nuclease (EMD Biosciences/Novagen catalog #70746), and 0.033 μl/mL of r-Lysozyme™ solution (EMD Biosciences/Novagen catalog #71110) to the cells. The cell suspensions were incubated at room temperature with gentle mixing for 15 min; spun at 14,000 rpm for 20 min (4° C.) and the supernatants were carefully removed. Total protein concentrations were determined using the Pierce BCA protein assay kit (Pierce catalog #23225) with bovine serum albumin as the standard and a microtiter plate format. The expression of the D-aminotransferase was analyzed using the Bio-Rad Experion Pro260 Automated Electrophoresis Station following the manufacturer's protocol with the cell extracts solutions diluted to 1 mg/mL.

The electrophoresis results show that the ArcticExpress™ system significantly increased the soluble expression of the SEQ ID NO:894 polypeptide when compared to the expression without chaperones or when co-expressed with the E. coli GroEL-GroES chaperones described in Example 16. The soluble expression was higher at lower temperatures, but still very high at 25° C.

Incubation Estimated Lane Sample Temperature DAT Expression 1 Pro260 Ladder 2 ArcticExpress(DE3)::894pMET1 15° C. 58% cell extract 3 ArcticExpress(DE3)::894pMET1 20° C. 46% cell extract 4 ArcticExpress(DE3)::894pMET1 25° C. 47% cell extract Activity Assay Protocol:

The enzymatic activity of the SEQ ID NO:894 polypeptide expressed in the ArcticExpress™ system was tested by following monatin formation in the presence of the aldolase described in Example 6. Each assay tube contained the following (in a total of 2 mL): 0.010 mg/mL aldolase in cell extract (assuming 20% soluble expression); 1.0 or 2.0 mg/mL of the SEQ ID NO:894 polypeptide in cell extract (assuming 50% soluble expression for the extract containing the SEQ ID NO:894 polypeptide) or purified B. sphaericus D-aminotransferase; 0.01% Tween-80; 200 mM sodium pyruvate; 100 mM D-tryptophan; 100 mM EPPS, pH 8.2; 1 mM MgCl₂; 0.05 mM PLP; and 10 mM potassium phosphate.

The reactions were incubated at room temperature in a Coy Laboratory Products, Inc. anaerobic chamber to minimize exposure to oxygen. All components except the enzymes were mixed together (the tryptophan did not completely dissolve until at least 1 h after the addition of the enzymes). The reactions were initiated by the addition of the enzymes. Samples were withdrawn at 1, 4, 7 and 22 h. Control reactions using 1 or 2 mg/mL purified B. sphaericus D-aminotransferase were also run. The concentrations of the substrates and products were measured as described in Example 3. The results are shown in Table 34. At 22 h, the concentration of monatin was 8.2 mM when the SEQ ID NO:894 polypeptide was present at 1 mg/mL and 10.5 mM at 2 mg/mL DAT polypeptide. When the B. sphaericus enzyme was added at 1 mg/mL, the concentration of monatin at 22 h was 10.7 mg/mL; at 2 mg/mL, the monatin concentration was 14.5 mM. The stereopurity (as determined by the FDAA derivatization protocol in Example 3) of the product was >98% R,R with both enzymes and enzyme concentrations. The concentration of the co-product HMG was significantly less when the SEQ ID NO:894 polypeptide was used (˜⅓ the concentration when compared to the assay samples containing B. sphaericus enzyme at either enzyme concentration). The HMG concentrations were evaluated by comparing the peak areas of the OPA derivatized samples.

TABLE 34 Monatin Formation (mM) Polypeptide (SEQ ID NO) 1 h 4 h 7 h 22 h 894 DAT (1 mg/mL) 0.9 2.3 3.6 8.3 894 DAT (2 mg/mL) 1.4 3.7 6.3 10.5 B. sphaericus DAT (1 mg/lmL) 1.0 4.2 6.1 10.7 B. sphaericus DAT (2 mg/lmL) 1.5 6.7 8.2 14.5

Example 18 Use of Stratagene ArcticExpress™ System to Increase the Soluble Expression of DATs

Transformation Protocol:

The plasmids SEQ ID NO:891/pET30a (encoding the SEQ ID NO:892 polypeptide), SEQ ID NO:873/pET30a (encoding the SEQ ID NO:874 polypeptide) and the Clostridium beijerinckii DAT (CbDAT) in pET30a were transformed into chemically competent Stratagene ArcticExpress™ (DE3) cells (catalog #230192) following the manufacturer's protocol. The cloning of these genes is described in Example 4 and assay results are in Example 9. The transformed cells were recovered in 0.5 mL of SOC medium for 1 h at 37° C. and plated on LB plates containing 100 mg/L ampicillin and 13 mg/L gentamycin. The plates were incubated at room temperature for 2 days and then stored at 4° C.

Expression Protocol:

Colonies from the transformation recovery plates were used to inoculate 5 mL of 2×YT medium containing 50 mg/L kanamycin and 13 mg/L gentamycin; the liquid cultures were incubated for 6 h at 30° C. Flasks of Novagen Overnight Express™ AutoinductionSystem 2 (EMD Biosciences/Novagen catalog #71366) containing solutions 1-6, with 100 mg/L ampicillin and 13 mg/L gentamycin were inoculated from the 5 mL cultures. After incubation at 30° C. for 5-6 h, the cultures were moved to a 15° C. incubator. The 15° C. incubations were continued until the OD₆₀₀ of the cultures reached 6 or greater. The cells were harvested by centrifugation, washed with cold 50 mM EPPS, pH 8.4, and then the cell pellets were frozen at −80° C.

Cell extracts were prepared by adding 5 mL per g of cell pellet of BugBuster (primary amine-free) Extraction Reagent (EMD Biosciences/Novagen catalog #70923) with 5 μL/mL of Protease Inhibitor Cocktail II (EMD Bioscience/Calbiochem catalog #539132), 1 μl/mL of Benzonase Nuclease (EMD Biosciences/Novagen catalog #70746), and 0.033 μl/mL of r-Lysozyme™ solution (EMD Biosciences/Novagen catalog #71110) to the cells. The cell suspensions were incubated at room temperature with gentle mixing for 15 min; spun at 14,000 rpm for 20 min (4° C.) and the supernatants were carefully removed. Total protein concentrations were determined using the Pierce BCA protein assay kit (Pierce catalog #23225) with bovine serum albumin as the standard and a microtiter plate format. The expression of the DAT was analyzed using the Bio-Rad Experion Pro260 Automated Electrophoresis Station following the manufacturer's protocol with the cell extracts solutions diluted to 1 mg/mL. The results are shown in Tables 35 and 36.

The electrophoresis results show that the SEQ ID NO:874 polypeptide expressed in a soluble form slightly better than the SEQ ID NO:892 polypeptide using the ArcticExpress™ system. The soluble expression of the Cb DAT varied depending on the colony picked in the transformation plate. None of these DAT polypeptides expressed in a soluble form using the ArcticExpress™ system as well as the SEQ ID NO:894 polypeptide described in Example 16 was expressed.

TABLE 35 Incubation Estimated DAT Lane Sample Temperature Expression 1 Pro260 Ladder 2 ArcticExpress(DE3)::891/pET30a 15° C. 11% cell extract (colony #1) 3 ArcticExpress(DE3)::891/pET30a 15° C. 9% cell extract (colony #2) 4 ArcticExpress(DE3)::873/pET30a 15° C. 15% cell extract (colony #1) 5 ArcticExpress(DE3)::873/pET30a 15° C. 10% cell extract (colony #2) 6 ArcticExpress(DE3)::891/pET30a 15° C. 9% cell extract (colony #1) 7 ArcticExpress(DE3)::891/pET30a 15° C. 8% cell extract (colony #2) 9 ArcticExpress(DE3)::873/pET30a 15° C. 15% cell extract (colony #1) 10 ArcticExpress(DE3)::873/pET30a 15° C. 11% cell extract (colony #2)

TABLE 36 Incubation Estimated DAT Lane Sample Temperature Expression 1 Pro260 Ladder 2 ArcticExpress(DE3)::CbDAT in 15° C. 9% pET30a cell extract (colony #1) 3 ArcticExpress(DE3)::CbDAT in 15° C. 19% pET30a cell extract (colony #2) 4 ArcticExpress(DE3)::CbDAT in 15° C. 13% pET30a cell extract (colony #3) 5 ArcticExpress(DE3)::CbDAT in 15° C. 4% pET30a cell extract (colony #4) Activity Assay Protocol

The enzymatic activity of the DAT polypeptides expressed in the ArcticExpress™ system were tested by following monatin formation in the presence of the aldolase described in Example 6. Each assay tube contained the following (in a total of 3 mL): 0.050 mg/mL aldolase in cell extract (estimating 20% soluble expression); 0.5 mg/mL DAT polypeptide in cell extract (estimating the soluble expression from the Experion data) or purified B. sphaericus DAT; 0.01 Tween-80; 200 mM sodium pyruvate; 100 mM D-tryptophan; 50 mM EPPS, pH 8.2; 1 mM MgCl₂; 0.05 mM PLP; and 10 mM potassium phosphate.

The reactions were incubated at room temperature in a Coy Laboratory Products, Inc. anaerobic chamber to minimize exposure to oxygen All components except the enzymes were mixed together (the tryptophan did not completely dissolve until at least 1 h after the addition of the enzymes). The reactions were initiated by the addition of the enzymes. Samples were withdrawn at 2, 4.5, 9 and 24 h. Control reactions with purified B. sphaericus D-aminotransferase were also run. The concentrations of the substrates and products were measured as described in Example 3. At 24 h, the assays containing the SEQ ID NO:892 or 894 polypeptide had produced approximately the same titer of monatin as the control B. sphaericus DAT reaction. The C. beijerinckii DAT reaction produced less than one-eighth of the monatin product, while the SEQ ID NO:874 polypeptide produced about half. The stereopurity of the product at 24 h (as determined by the FDAA derivatization protocol in Example 3) was 96% R,R monatin or greater for the SEQ ID NO:892 polypeptide. The concentration of the by-product HMG (4-hydroxy-4-methyl glutamic acid) was measured for the reactions with the SEQ ID NO:892, the SEQ ID NO:894 and the B. sphaericus DAT polypeptides. The assay with polypeptides having the sequence shown in SEQ ID NO:894 produced far less of the HMG by-product than the other two (about 20% of that produced by the assay containing the B. sphaericus DAT and about 40% of that produced by the assay with the SEQ ID NO:892 polypeptide). The HMG concentrations were estimated by comparing the peak areas of the OPA post-column derivatized samples.

TABLE 37 Monatin Formation (mM) Polypeptide (SEQ ID NO) 2 h 4.5 h 9 h 24 h C. beijerinckii DAT 0.3 0.7 0.8 0.9 874 DAT 1.8 3.2 4.1 4.4 892 DAT 1.6 3.7 5.4 8.4 894 DAT 1.4 2.8 4.5 8.4 B. sphaericus DAT 1.2 2.9 4.3 8.1

These results indicate that when expressed under the appropriate conditions, the polypeptides having the sequence of SEQ ID NO:892 and 894 can be utilized in reactions to produce highly pure R,R monatin at a titer as high as the positive control enzyme.

Example 19 Evaluation of Alternative Expression Hosts to Increase the Soluble Expression of a DAT

Because the soluble expression of the SEQ ID NO:894 polypeptide was low when the nucleic acid was expressed in BL21(DE3) (see Example 8), alternative expression hosts were evaluated for improving soluble expression. The OverExpress™ C41(DE3) and C43(DE3) hosts contain genetic mutations phenotypically selected for conferring toxicity tolerance and express some toxic proteins at higher titers than other E. coli hosts.

Transformation Protocol

The plasmid SEQ ID NO:893/pET30a was transformed into electrocompetent cells of the OverExpress™ C41(DE3) and C43(DE3) (Lucigen catalog #60341 and 60345, respectively) using a Bio-Rad Gene Pulsar II system following the manufacturer's protocol. The transformation mixtures were recovered in 1 mL SOC medium for 1 hr at 37° C. and plated on LB plates containing 50 mg/L kanamycin. The plates were incubated overnight at 37° C. Multiple colonies were patched onto fresh plates and analyzed for the appropriate insert size using colony PCR. A small aliquot of cells was suspended in 0.025 mL H₂O and incubated at 95° C. for 10 min. After cooling, 2 μL of each of the suspensions were used as the template in 0.025 mL reactions, each also containing 0.5 μL T7 promoter primer (0.1 mM), 0.5 μL T7 terminator primer (0.1 mM), 0.5 μL PCR Nucleotide Mix (Roche #12526720; 10 mM each nucleotide), 2.5 μL Roche Expand DNA polymerase buffer #2, and 0.5 μL Expand DNA polymerase (Roche Expand High Fidelity PCR System catalog #1732650). The 3-step thermocycler program was run for 25-30 cycles: 1 min at 94° C.; 1 min at 54° C., 1.3 min at 72° C. with a final polishing step of 7 min at 72° C.

Expression Studies

Flasks of Novagen Overnight Express™ AutoinductionSystem 2 (EMD Biosciences/Novagen catalog #71366) containing solutions 1-6 and 50 mg/L kanamycin (40 mL in each flask) were inoculated from the patch plates of the transformed C41(DE3) and C43(DE3) cells (2 patches for each of the transformations). The cells were incubated at 30° C. overnight and harvested by centrifugation when the OD₆₀₀ reached 6 or greater. The cells were washed with cold buffer, were centrifuged again, and either used immediately or frozen at −80° C.

Cell extracts were prepared by adding 5 mL per g of cell pellet of BugBuster® (primary amine-free) Extraction Reagent (EMD Biosciences/Novagen catalog #70923) with 5 μL/mL of Protease Inhibitor Cocktail II (EMD Bioscience/Calbiochem catalog #539132), 1 μl/mL of Benzonase® Nuclease (EMD Biosciences/Novagen catalog #70746), and 0.033 μl/mL of r-Lysozyme™ solution (EMD Biosciences/Novagen catalog #71110) to the cells. The cell suspensions were incubated at room temperature with gentle mixing for 15 min; spun at 14,000 rpm for 20 min (4° C.) and the supernatants carefully removed. Total protein concentrations were determined using the Pierce BCA protein assay kit (Pierce catalog #23225) with bovine serum albumin as the standard and a microtiter plate format. The expression of the DAT polypeptide was analyzed by SDS-PAGE using Bio-Rad Ready Gel® Precast 4-15% polyacrylamide gradient gels (Bio-Rad Laboratories catalog #161-1104). BioRad SDS-PAGE low range standards (catalog #161-0304) were run as standards on each gel. Aliquots of the cell extracts (15 μg protein) were mixed with protein loading buffer containing 2% SDS, 10% glycerol, 12.5% 2-mercaptoethanol, 0.1% bromophenol blue and 62.5 mM Tris-HCl (pH 8), incubated at 95° C. for 5 min, cooled and then loaded on the gel. In addition, the combined soluble and insoluble protein expression (total protein) was analyzed for the transformants. A 10 μl aliquot of each cell suspension before centrifugation was diluted in 90 μL protein loading buffer, incubated at 95° C. for 10 min, and cooled. Ten μL of each cooled solution was loaded on the gel.

The electrophoresis gel shows that the protein expressed better in the C41(DE3) host than in the C43(DE3) host, however the apparent soluble expression was not higher than when BL21(DE3) cells were used.

Example 20 Evaluation of Low Temperature Expression to Increase the Soluble Expression of DAT

Because the soluble expression of SEQ ID NO:894 D-aminotransferase was low when the gene was expressed in the E. coli strain BL21(DE3) (see Example 8), the gene was inserted in vectors with cold shock Protein A promoters to evaluate low temperature expression.

The Takara pCold Expression Vectors are four different vectors that utilize the cold shock Protein A (cspA) promoter for expression of high purity, high yield recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15° C.) where the synthesis of other proteins is suppressed and protease activity is decreased. In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (amp′), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Three of the vectors also contain a translation enhancing element (TEE), a His-Tag sequence, and/or Factor Xa cleavage site.

Cloning Protocol

The SEQ ID NO:893 DAT nucleic acid from plasmid SEQ ID NO:893/pET30a (encoding the polypeptide having the sequence of SEQ ID NO:894) was subcloned into the Takara pCold vectors at the NdeI and XhoI sites of the pCOLDII (contains a TEE and a His-tag sequence), pCOLDIII (contains a TEE) and pCOLDIV vectors. The digested vector and insert bands were gel purified using a QIAquick Gel Extraction Kit (Qiagen catalog #28704) and ligated using a Roche Rapid DNA Ligation Kit (catalog #1635379) following the manufacturer's protocol. The ligation mixtures were transformed into Invitrogen OneShot TOP10 chemically competent cells (catalog #C404003) by heat shock at 42° C. After recovery in 500 μL SOC medium for 1 h at 37° C., the transformation mixtures were plated on LB plates containing 100 mg/L ampicillin and incubated at 37° C. overnight. Colonies were picked from the transformation plates and used to inoculate 5 mL cultures of LB containing 100 mg/mL ampicillin that were incubated overnight at 37° C. Plasmid DNA was purified from the 5 mL cultures using a QIAprep® Spin Miniprep Kit (Qiagen catalog #27104). The inserts were verified restriction digestion with NdeI and XhoI by sequencing (Agencourt Bioscience Corp, Beverly, Mass.).

The SEQ ID NO:894dat pCOLD plasmids were transformed into chemically competent Stratagene ArcticExpress™ (DE3) cells and Novagen BL21(DE3) cells following the manufacturers' protocols. The transformation mixtures were recovered in 0.5-1 mL SOC medium for 1 h at 37° C. and plated on LB plates containing 100 mg/mL ampicillin and 13 mg/L gentamycin (ArcticExpress™ (DE3)) or 100 mg/mL ampicillin (BL21(DE3)). The plates were incubated overnight at 37° C.

Expression Studies

Flasks of Novagen Overnight Express™ AutoinductionSystem 2 (EMD Biosciences/Novagen catalog #71366) containing solutions 1-6 and 100 mg/L ampicillin and 13 mg/L gentamycin (ArcticExpress™ (DE3)) or 100 mg/mL ampicillin (BL21(DE3) were inoculated from the patch plates of the transformed cells (2 patches for each of the SEQ ID NO:893/pCOLDII, SEQ ID NO:893/pCOLDIII and SEQ ID NO:893/pCOLDIV transformations).

After incubation at 30° C. for 3-5 hr the cultures were moved to a 15° C. incubator. The 15° C. incubations were continued until the OD at 600 nm of the cultures reached 6 or greater. The cells were harvested by centrifugation, washed with cold buffer, and then the cell pellets were frozen at −80° C.

Cell extracts were prepared by adding 5 mL per g of cell pellet of BugBuster® (primary amine-free) Extraction Reagent (EMD Biosciences/Novagen catalog #70923) with 5 μL/mL of Protease Inhibitor Cocktail II (EMD Bioscience/Calbiochem catalog #539132), 1 μl/mL of Benzonase® Nuclease (EMD Biosciences/Novagen catalog #70746), and 0.033 μl/mL of r-Lysozyme™ solution (EMD Biosciences/Novagen catalog #71110) to the cells. The cell suspensions were incubated at room temperature with gentle mixing for 15 min; spun at 14,000 rpm for 20 min (4° C.) and the supernatants were carefully removed. Total protein concentrations were determined using the Pierce BCA protein assay kit (Pierce catalog #23225) with bovine serum albumin as the standard and a microtiter plate format. The expression of the D-aminotransferase was analyzed using the Bio-Rad Experion™ Pro260 Automated Electrophoresis Station following the manufacturer's protocol with the cell extract solutions diluted to 1 mg/mL. The results are shown in Tables 38 and 39.

TABLE 38 Estimated % DAT Lane Sample Expression L Pro260 Ladder 1 BL21(DE3)::SEQ ID NO: 893/pCOLDII#1 8.7 2 BL21(DE3):: SEQ ID NO: 893/pCOLDII#2 8.5 3 ArcticExpress ™(DE3):: SEQ ID NO: 893/ 9.8 pCOLDII#1 4 ArcticExpress ™(DE3):: SEQ ID NO: 893/ 6.4 pCOLDII#2

TABLE 39 Estimated % Lane Sample DAT Expression L Pro260 Ladder 1 BL21(DE3):: SEQ ID NO: 893/pCOLDIII#1 4.3 2 BL21(DE3):: SEQ ID NO: 893/pCOLDIII#2 2.3 3 BL21(DE3):: SEQ ID NO: 893/pCOLDIV#1 14.6 4 BL21(DE3):: SEQ ID NO: 893/pCOLDIV#2 14.2 5 BL21(DE3):: SEQ ID NO: 893/pCOLDIII#1 4.4 6 BL21(DE3):: SEQ ID NO: 893/pCOLDIII#2 5.2 7 BL21(DE3):: SEQ ID NO: 893/pCOLDIV#1 13.8 8 BL21(DE3):: SEQ ID NO: 893/pCOLDIV#2 16.1 9 BL21(DE3):: SEQ ID NO: 893/pCOLDIV#1 16.2 10 BL21(DE3):: SEQ ID NO: 893/pCOLDIV#2 16.8

The Experion Pro260 results show that the SEQ ID NO:894 DAT protein expressed better when the nucleic acid was incorporated in the pCOLDIV vector than in either the pCOLDII or pCOLDIII vector. From the experiments shown above, the average expression level for SEQ ID NO:893/pCOLDII was about 8%, regardless of expression host used; the average expression level for SEQ ID NO:893/pCOLDIII was about 4%, while the average expression level for SEQ ID NO:893/pCOLDIV was ˜15%. These expression levels are significantly less than those described in Examples 16 when the SEQ ID NO:893 nucleic acid was co-expressed with the GroEL-GroES chaperones and in Example 17 when the nucleic acid was expressed in the Stratagene ArcticExpress™ system.

Example 21 Codon Modification of a DAT

An attempt to improve the solubility of the SEQ ID NO:894 polypeptide expressed in E. coli was undertaken with the presumption that, slowing the rate of translation in E. coli would allow more time for proper folding of the SEQ ID NO:894 polypeptide, thereby giving a higher expression of soluble protein. A BLAST search (NCBI) of the SEQ ID NO:894 polypeptide sequence revealed that some of the most closely related public sequences were from Clostridium species, specifically Clostridium beijerinckii. Example 9 describes the results from cloning, expressing, and assaying the CbDAT and its use in monatin formation reactions. Specifically, expression was high in the total protein fraction but very low in the soluble protein fraction.

The codon usage tables of C. beijerinckii and E. coli K12 were compared. Several rarely used codons in C. beijerinckii were found to be highly abundant in E. coli K12 (Table 40). It is possible that these rare codons cause translational pauses in C. beijerinckii, whereas in an E. coli K12 host, there may not be a pause. In the SEQ ID NO:894 sequence, 4 doublets were identified in which tandem rare codons for C. beijerinckii had become “non-rare” (i.e. abundant) in E. coli K12. The goal was to change these codons into rare codons for expression in E. coli K12 host using the E. coli K12 codon usage table. Primers were designed to change these doublets. SEQ ID NO:893/pET30a (described in Example 4) was used as a template and mutation was carried out according to the Stratagene QuickChange kit instructions. The primers utilized to modify the SEQ ID NO:893 nucleic acid sequence are shown below, along with the native gene (the targeted doublet sequences are underlined).

TABLE 40 Codon Usage Codon Original (per thousand) Altered Usage Codons C. beijerinckii E. coli Codons E. coli GCC 3.7 25.6 GCT 15.3 CTG 1.4 52.9 CTA 3.9 ACC 2.5 23.5 ACA 7.0 CGC 0.8 22.0 CGA 3.5 GCG 2.9 33.8 GCT 15.3 CCG 1.1 23.3 CCC 5.4

native sequence >SEQ ID NO: 893 ATGGACGCACTGGGATATTACAACGGAAAATGGGGGCCTCTGGACGAGATGACCGTGCCGATGAACGACAG GGGTTGTTTCTTTGGGGACGGAGTGTACGACGCTACCATCGCCGCTAACGGAGTGATCTTTGCCCTGGACGAGCACA TTGACCGGTTTTTAAACAGCGCAAAGCTCCTGGAAATAGAAATCGGTTTTACAAAAGAGGAATTAAAAAAAACTTTT TTTGAAATGCACTCCAAAGTGGATAAAGGGGTGTACATGGTTTATTGGCAGGCGACTCGCGGAACAGGCCGTCGAAG CCATGTATTTCCGGCAGGTCCCTCAAATCTCTGGATTATGATTAAGCCCAATCACGTCGACGATCTTTATAGAAAAA TCAAGCTCATTACCATGGAAGATACCCGCTTCCTCCACTGCAACATCAAGACCCTTAACCTTATTCCCAATGTCATT GCCTCCCAGCGGGCGCTGGAAGCGGGCTGCCACGAGGCGGTCTTTCACCGGGGTGAAACAGTAACCGAGTGCGCCCA CAGCAATGTCCACATCATTAAAAACGGCAGGTTTATCACCCACCAGGCGGACAACCTAATCCTTCGGGGCATAGCCC GTAGCCATTTATTGCAAGCCTGTATCAGGCTGAACATTCCATTTGACGAACGGGAATTTACCCTTTCGGAATTATTT GACGCGGATGAGATTCTTGTGTCCAGCAGCGGCACACTCGGCCTTAGCGCCAATACAATTGATGGAAAAAACGTGGG GGGAAAAGCGCCGGAACTGCTAAAAAAAATTCAGGGCGAAGTGTTGAGGGAATTTATCGAAGCGACAGGCTACACGC CTGAGTGGAGCACAGTATAG Primers for Doublet 1 mutant (SEQ ID NO: 1074) CTAACGGAGTGATCTTTGCTCTAGACGAGCACATTGAC (SEQ ID NO: 1075) GTCAATGTGCTCGTCTAGAGCAAAGATCACTCCGTTAG Primers for Doublet 2 mutant (SEQ ID NO: 1076) CATGGAAGATACACGATTCCTCCACTGCAACATCAAGAC (SEQ ID NO: 1077) GTCTTGATGTTGCAGTGGAGGAATCGTGTATCTTCCATG Primers for Doublet 3 mutant (SEQ ID NO: 1078) ATTGCCTCCCAGCGGGCTCTAGAAGCGGGCTGCCACG (SEQ ID NO: 1079) CGTGGCAGCCCGCTTCTAGAGCCCGCTGGGAGGCAAT Primers for Doublet 4 mutant (SEQ ID NO: 1080) GGGGGGAAAAGCTCCCGAACTGCTAAAAAAAATTCAGG (SEQ ID NO: 1081) CCTGAATTTTTTTTAGCAGGTCGGGAGCTTTTCCCCCC

Clones with the correctly altered sequence were transformed into BL21(DE3) host for enzyme expression assays. Enzyme expression was determined by growing the cells overnight in Overnight Express II and lysing the cells with BugBuster reagent followed by SDS PAGE analyses of crude cell extract and soluble protein.

It appeared that there was a slight improvement in soluble protein expression with codon changes to doublets 1, 2 and 3. Codon changes at doublet 4 were not beneficial for soluble protein expression. Codon changes for doublets 1, 2 and 3 were combined in pairs using the Stratagene QuickChange kit and the primers designed for the initial codon changes. Clones with the correctly altered sequence were transformed into BL21(DE3) host for enzyme expression assays. Enzyme expression was determined by growing the cells overnight in Overnight Express II and lysing the cells with BugBuster reagent followed by SDS PAGE analyses of crude cell extract and soluble protein. The combinations of mutations to doublets 1 and 2, 2 and 3 and 1 and 3 yielded soluble protein bands visible on an SDS-PAGE gel. However, there still appeared to be more protein in the total protein fractions.

Example 22 The Evaluation of Periplasmic Expression of a DAT Polypeptide

Because the soluble expression of the SEQ ID NO:894 polypeptide was low when the gene product was expressed as a cytoplasmic protein in the E. coli host BL21(DE3) (see Example 8), the gene was cloned into vectors to generate fusion proteins that should be exported into the periplasmic space. The periplasm provides conditions that promote proper folding and disulfide bond formation and may enhance the solubility and activity of certain target proteins.

Cloning into EMD Biosciences/Novagen pET26b allows production of the target protein with a periplasmic signal sequence. The signal sequence is cleaved by signal peptidase concomitant with export. The EMD Biosciences/Novagen pET39b and pET40b are designed for cloning and expression of target proteins fused with a 208 amino acid DsbA-Tag™ or 236 amino acid DsbC-Tag™. DsbA and DsbC are periplasmic enzymes that catalyze the formation and isomerization of disulfide bonds, respectively. The fusion proteins are typically localized in the periplasm.

Cloning Protocol

The SEQ ID NO:893 nucleic acid from plasmid SEQ ID NO:893/pET30a (described in Example 4) was cloned into the EMD Biosciences/Novagen pET26b (catalog #69862-3), pET39b (catalog #70090-3), and pET40b (catalog #70091-3) vectors at the EcoRI and NotI sites of the vectors. The DATs nucleic acid with a 5′ EcoRI site and a 3′ NotI site was generated using the amplification protocol described in Example 4 and the following primers:

(SEQ ID NO: 1082) 5′-CGCA

GGACGCACTGGGATATTACAAC-3′ (SEQ ID NO: 1083) 5′-GTTA

TATACTGTGCTCCACTCAG-3′

The restriction sites are in italics in the primer sequences. The resulting DNA product and the pET26b, pET39b and pET40b vectors were digested with EcoR1 and Not I (New England Biolabs) following the suggested manufacturer's protocol. The vector reaction mixtures were subsequently treated with Shrimp Alkaline Phosphatase (Roche catalog #1758250). The digested vector and insert bands were gel purified from a 1% agarose gel using a Qiagen QIAquick® Gel Extraction Kit (catalog #28704) and ligated using a Roche Rapid Ligation Kit (catalog #1635379) following the manufacturer's protocol. The ligation mixtures were transformed into Invitrogen OneShot® TOP10 chemically competent cells (catalog #C404003) by heat shock at 42° C. After recovery in 500 μL SOC medium for 1 h at 37° C., the transformation mixtures were plated on LB plates containing 50 mg/L kanamycin and incubated at 37° C. overnight. Colonies were picked from the transformation plates and used to inoculate 5 mL cultures of LB containing 50 mg/mL kanamycin that were incubated overnight at 37° C. Plasmid DNA was purified from the 5 mL cultures using a Qiagen QIAprep spin miniprep kit (catalog #27104). The nucleic acid sequences were verified by sequencing (Agencourt Bioscience Corp, Beverly, Mass.). Plasmids with the correct insert sequences were transformed into EMD Biosciences/Novagen BL21(DE3) chemically competent cells (catalog #69450) by heat shock as described above.

Expression Studies

Flasks of Novagen Overnight Express™ AutoinductionSystem 2 (EMD Biosciences/Novagen catalog #71366) containing solutions 1-6 and 50 mg/L kanamycin (50 mL in each flask) were inoculated from fresh plates of BL21(DE3) cells carrying the SEQ ID NO:893 DAT nucleic acid (encoding the polypeptide of SEQ ID NO:894) in either pET26b, pET39b or pET40b. The cells were incubated at 30° C. overnight and harvested by centrifugation when the OD₆₀₀ reached 6 or greater. The cells were washed with cold buffer, were centrifuged again, and used immediately or the cell pellets were frozen at −80° C. Before harvesting, 2 mL culture aliquots were withdrawn from each flask for soluble and total protein (soluble and insoluble) expression analyses. Cell extracts were prepared as described in Example 16. Total protein samples were prepared by suspending a small amount of cell pellet in protein loading buffer containing 2% SDS, 10% glycerol, 12.5% 2-mercaptoethanol, 0.1% bromophenol blue and 62.5 mM Tris-HCl, pH 8, and incubating at 95° C. for 10 min.

The periplasmic cellular fractions were prepared from the remainder of the cells from each culture following the protocol described in the EMD Biosciences/Novagen pET System Manual. The resulting fractions were concentrated 30-fold using Amicon Ultracel 10 k centrifugal filter devices (Millipore catalog #UFC901024). Total protein concentrations of the cell extracts and the periplasmid fractions were determined using the Pierce BCA protein assay kit (Pierce catalog #23225) with Bovine Serum Albumin as the standard and a microtiter plate format. Fifteen μg protein samples of the cell extracts and 10 μg protein samples of the periplasmic fractions were analyzed by SDS-PAGE using Bio-Rad Ready Gel® Precast 4-15% polyacrylamide gradient gels (Bio-Rad Laboratories catalog #161-1104). In addition, the total protein samples were analyzed by SDS-PAGE. BioRad SDS-PAGE low range standards (catalog #161-0304) were run as standards on each gel.

Analysis of the total protein SDS-PAGE gel shows that proteins with the predicted molecular weights expressed using the Overnight Express™ AutoinductionSystem 2. However, analysis of the SDS-PAGE gel loaded with the cell extract fractions or with the periplasmic fractions suggests that these proteins did not express solubly nor were they exported into the periplasm.

Example 23 Production of Monatin from Indole-3-pyruvate

The maximum concentration of monatin obtained when D-tryptophan and pyruvic acid are the starting raw materials in the monatin formation assay described in Example 5 is limited by the solubility of tryptophan. In order to explore the potential of using an aldolase and the SEQ ID NO:220 DAT polypeptide (described in Example 14) in reaching higher monatin concentrations, the reaction starting with indole-3-pyruvatr (I3P) and pyruvate acid as raw materials were analyzed. In this case, it was also necessary to provide an amino donor such as D-alanine or both D-alanine and D-tryptophan.

The test was conducted using purified SEQ ID NO:220 DAT polypeptide (production and purification described in Example 15) and an aldolase (described in Example 6). The reaction was set up as follows (in a total of 1 mL): 200 mM Indole-3-pyruvate (I3P); 200 mM sodium pyruvate; 400 mM D-alanine; 100 mM EPPS, pH 8.0; 1 mM MgCl₂; 0.05 mM PLP; and 10 mM potassium phosphate.

Both enzymes were added in excess to facilitate conversion to monatin to minimize completion from non-enzymatic degradation reactions. The reactions were incubated at room temperature in a Coy Laboratory Products, Inc. anaerobic chamber to minimize exposure to oxygen All components except the enzymes were mixed together and the pH was adjusted to 8.0. The reactions were initiated by the addition of the enzymes (0.04 mg/mL aldolase as cell extract (assuming 20% expression) and 0.40 mg/mL purified SEQ ID NO:220 DAT polypeptide).

In some tests as indicated in the table below, D-tryptophan was also added at either 50 or 100 mM in addition to the D-alanine to act as amino donor and also to limit the amount of indole-3-pyruvate consumed in the formation of D-tryptophan. The monatin formation was measured after 18 hours using the LC/MS/MS methodology described in Example 3, and the results are presented in Table 41 below.

TABLE 41 Monatin Formation from I3P (mM) Reactant initial concentrations (mM) Monatin concentration (mM) 200 I3P; 200 pyr: 400 D-ala 44.9 200 I3P; 200 pyr: 400 D-ala, 50 D-trp 47.8 200 I3P; 200 pyr: 400 D-ala, 100 D-trp 61.0

As shown above, the aminotransferase and aldolase enzymes were active at the higher reactant concentrations and a much higher monatin concentration was achieved.

At 18 h, while using 200 mM indole-3-pyruvate, 200 mM sodium pyruvate and 100 mM D-tryptophan, the concentration of monatin was 61 mM. A small increase in monatin production (47.8 mM) was observed under the conditions of the assay, with the addition of 50 mM D-tryptophan vs. just using 400 mM D-alanine.

Example 24 Homology Table

Table 42 shows some of the most active DAT polypeptides and the corresponding closest homologs from the published databases or literature.

TABLE 42 DAT polypeptide % (SEQ Sequence ID NO) Closest Hit from Database Identity 896 Bacillus sp. YM-1 76 874 Serine glyoxylate transaminase from 51 Acidiphilium cryptum JF-5 (NR Accession No: 148260372) 878 Putative glutamate-1-semialdehyde 2,1- 43 aminomutase from Planctomyces maris DSM 8797 (NR Accession No. 149173540) 882 D-alanine transaminase from Oceanobacter sp. 57 RED65 (NR Accession No: 94500389) 910 DAT from B. macerans 91 176 D-amino acid aminotransferase from 62 Clostridium beijerincki (NCIMB 8052) 220 D-amino acid aminotransferase from 62 Clostridium beijerincki (NCIMB 8052) 156 Aminotransferase class-III (leadered) from 46 Chloroflexus aggregans DSM 9485 (NR Accession No: 118045454) 214 D-amino acid aminotransferase from 61 Clostridium beijerincki (NCIMB 8052) 918 Aminotransferase class IV from Robiginitalea 57 biformata HTCC2501 (NR Accession No: 88806011) 902 Putative glutamate-1-semialdehyde 2;1- 46 aminomutase from Planctomyces maris DSM 8797 (same protein as above) 884 D-alanine transaminase from Thiobacillus 40 denitrificans ATCC 25259 (NR Accession No: 74316285) 866 D-alanine aminotransferase from Lactobacillus 49 salivarius subsp. salivarius UCC118 946 D-amino acid aminotransferase from 63 Clostridium beijerincki (NCIMB 8052)

As shown in Example 9, homologs of the polypeptides having the sequence shown in SEQ ID NO:866, 946, 220, and 176 were cloned and also had activity in the production of R,R monatin, despite a sequence identity among those polypeptides of between 49% and 63%. Similarly, the predicted D-alanine transaminase from Oceanobacter species and the Robinginitalea biformata aminotransferase are expected to have broad D-amino acid aminotransferase activity like the DAT polypeptides having the sequence of SEQ ID NO:882 and 918.

Appendix I shows a table that describes selected characteristics of exemplary nucleic acids and polypeptides of the invention, including sequence identity comparison of the exemplary sequences to public databases. By way of example and to further aid in understanding Appendix I, the first row, labeled “SEQ ID NO:”, the numbers “1, 2” represent the exemplary polypeptide of the invention having a sequence as set forth in SEQ ID NO:2, encoded by, e.g., SEQ ID NO:1. The sequences described in Appendix I (the exemplary sequences of the invention) have been subject to a BLAST search (as described herein) against two sets of databases. The first database set is available through NCBI (National Center for Biotechnology Information). The results from searches against these databases are found in the columns entitled “NR Description”, “NR Accession Code”, “NR E-value” or “NR Organism”. “NR” refers to the Non-Redundant nucleotide database maintained by NCBI. This database is a composite of GenBank, GenBank updates, and EMBL updates. The entries in the column “NR Description” refer to the definition line in any given NCBI record, which includes a description of the sequence, such as the source organism, gene name/protein name, or some description of the function of the sequence. The entries in the column “NR Accession Code” refer to the unique identifier given to a sequence record. The entries in the column “NR E-value” refer to the Expected value (E-value), which represents the probability that an alignment score as good as the one found between the query sequence (the sequences of the invention) and that particular database sequence would be found in the same number of comparisons between random sequences as was done in the present BLAST search. The entries in the column “NR Organism” refer to the source organism of the sequence identified as the closest BLAST hit.

The second database set is collectively known as the GENESEQ™ database, which is available through Thomson Derwent (Philadelphia, Pa.). The results from searches against this database are found in the columns entitled “GENESEQ Protein Description”, “GENESEQ Protein Accession Code”, “E-value”, “GENESEQ DNA Description”, “GENESEQ DNA Accession Code” or “E-value”. The information found in these columns is comparable to the information found in the NR columns described above, except that it was derived from BLAST searches against the GENESEQ™ database instead of the NCBI databases.

In addition, this table includes the column “Predicted EC No.”. An EC number is the number assigned to a type of enzyme according to a scheme of standardized enzyme nomenclature developed by the Enzyme Commission of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (IUBMB). The results in the “Predicted EC No.” column are determined by a BLAST search against the Kegg (Kyoto Encyclopedia of Genes and Genomes) database. If the top BLAST match has an E-value equal to or less than e-6, the EC number assigned to the top match is entered into the table. The columns “Query DNA Length” and “Query Protein Length” refer to the number of nucleotides or the number amino acids, respectively, in the sequence of the invention that was searched or queried against either the NCBI or GENESEQ™ databases. The columns “Subject DNA Length” and “Subject Protein Length” refer to the number of nucleotides or the number amino acids, respectively, in the sequence of the top match from the BLAST searches. The results provided in these columns are from the search that returned the lower E-value, either from the NCBI databases or the GENESEQ™ database. The columns “% ID Protein” and “% ID DNA” refer to the percent sequence identity between the sequence of the invention and the sequence of the top BLAST match. The results provided in these columns are from the search that returned the lower E-value, either from the NCBI databases or the GENESEQ™ database.

Part C Example 25 Construction and Testing of GSSM^(SM) Mutants

This example describes the construction of exemplary nucleic acids and polypeptides, and describes their enzymatic activity. The nucleotide sequence (SEQ ID NO:219) was subcloned into pSE420-C-His vector and expressed in E. coli XL1-BLUE host (Stratagene, La Jolla, Calif.) to produce the exemplary D-aminotransferase (DAT) enzyme having the amino acid sequence shown in SEQ ID NO:220. The pSE420-C-His vector was created by adding a C-terminal His-tag to the pSE420 vector from Invitrogen (Carlsbad, Calif.). Construct SEQ ID NO:220 (in E. coli XL1-BLUE), was used as a starting sequence into which modifications were introduced and is referred to herein as the wild type (WT) sequence. A first round of modification (i.e., single-residue mutations) was performed using Gene Site Saturated Mutagenesis^(SM) (GSSM^(SM)) technology (see, for example, U.S. Pat. No. 6,171,820). The mutants made using GSSM^(SM) technology were expressed in the pSE420-C-His vector in E. coli host XL1-BLUE, arrayed into 384-well plates and grown at 37° C. overnight. Samples were subcultured and grown at 30° C. for two nights (36-48 hours). Cultures were frozen at −20° C. until cell lysates could be prepared.

Cells were lysed by addition of 10 μL of BPER II (Thermo Scientific, Rockford, Ill.) to each well. Samples were mixed three times and lysed on ice for one hour. Crude lysates were then assayed in the primary screen. 25 μL of crude lysate was assayed with 1 mM R,R-monatin, 25 mM pyruvic acid sodium salt, 0.08 mM PLP in 50 mM sodium phosphate (pH 8) at room temperature. After three hours, an aliquot was taken and formic acid was added to a final concentration of 2%. Samples were diluted with water to be within the range of the standard curve. Samples were analyzed for monatin consumption and alanine formation using the LC/MS/MS methods described in Example 1 (LC/MS/MS Method for Detecting D-alanine or R,R-monatin). Sample performance was compared to the performance of the wild type control (i.e., SEQ ID NO:220).

Mutants that outperformed the wild type control were selected as hits from the GSSM^(SM) primary screen. Glycerol stocks of the primary hits were used to inoculate new 384-well plates. The hits were arrayed in quadruplicate, grown and lysed as indicated for the primary screen. The primary hits were then tested in a secondary screen. The secondary screen method was the same as for the primary screen except the mutants were tested with 1 mM and 15 mM R,R-monatin substrate. Samples were analyzed for monatin consumption and alanine formation using LC/MS/MS. Sample performance was compared to the performance of the wild type control.

Sample performance was evaluated using a scoring system based on alanine production and monatin consumption. The maximum score for a single sample was six. A maximum of three points were assigned for alanine production and a maximum of three points were assigned for monatin consumption. The scoring criteria were as follows: 1 point assigned for a value between average and one standard deviation of the positive control; 2 points assigned for a value between one and two standard deviations of the positive control; and 3 points assigned for a value beyond two standard deviations of the positive control.

The highest potential total score for a mutant was 48 (since the samples were screened in quadruplicate at 1 and 15 mM monatin). In general, mutants scoring 20 points or more were selected as secondary hits. However, some exceptions were made for samples scoring less than 20 points. Samples with alanine formation and monatin consumption values on the verge of the threshold requirements were also selected as hits. This prevented the premature elimination of hits and allowed for further testing and characterization in the tertiary screen.

Samples identified as secondary screen hits, using the criteria above, are listed in Table 43. Secondary hits were streaked from glycerol stocks onto LB agar plates containing 100 μg/mL carbenicillin and grown overnight at 37° C. Single colonies were used to inoculate 1 mL LB containing carbenicillin (100 μg/mL). Cultures were grown overnight at 37° C. DNA was isolated from the cultures, and then prepared and sequenced using 3730XL automated sequencers (Applied Biosystems, Foster City, Calif.).

Mutations and the amino acid position of the mutation for secondary hits are listed below in Table 43. Numbering of the amino acid positions starts with the N-terminal methionine. For example, the first mutation listed “Y6L” refers to changing the tyrosine in amino acid position 6 of the wild type enzyme (SEQ ID NO:220) to leucine. At the nucleic acid level, any codon which codes for the desired (mutated) amino acid can be used.

All of the amino acid sequences described in Tables 43, 44 and 50, below, are exemplary polypeptides; also provided are nucleic acid sequences that encode such polypeptides.

Example 26 List of GSSM^(SM) Mutations

TABLE 43 GSSM^(SM) Mutants Identified as Secondary Screen Hits Mutant name Mutation   1 Y6L   2 Y6C; SILENT MUTATION AT AA31 (GGC → GGT)   3 Y6F   4 Y6L   5 Y6H   6 Y6L   7 Y6M   8 N10S   9 N10W  10 N10T  11 N10R  12 N10T  13 L14V  14 L14L  15 G41G  16 T18W  17 N40N  18 V19T  19 V42V  20 I62C  21 V82A  22 A57M  23 V42M  24 G41Y  25 A45L  26 V93Y  27 V93G  28 L46A  29 L46H  30 G98A  31 P20S  32 V93A  33 V103T  34 P108F  35 V93L  36 S101S  37 A106G  38 S101Q  39 P108T  40 N118G  41 P108C  42 I120L  43 A106W  44 N118R  45 N110A; N118G  46 N118A  47 N118R  48 P117W; N118K  49 D133N  50 K126Q  51 K126R  52 K128S  53 I127M  54 T131T  55 D133L  56 M132A  57 D133E  58 L129V  59 K126K  60 I130M  61 M132Y  62 K128R  63 M132R  64 L129I  65 K128L; D2D (GAC → GAT)  66 F137W  67 I152V  68 N55L  69 N150S  70 L138L  71 P149P  72 G161G  73 A165T  74 H163R  75 H163K  76 H168A  77 E171S  78 E171R  79 E171R  80 T172I  81 C176G  82 A177S  83 A177S  84 S80L; R156W  85 H182G  86 N186S  87 K185R  88 K185T  89 D2H  90 D2T; E260G  91 D2Y  92 D2G  93 D2Q  94 D2F  95 D2A  96 D2T  97 D2N  98 D2R  99 D2I 100 D2V; G9A 101 G12A 102 D47W 103 S56S 104 I64H 105 L66L 106 I64C 107 L66G 108 E69Y 109 T74L 110 K73L 111 T74V 112 T74M 113 T74R 114 T74A 115 N76C 116 E77R 117 R156A 118 K72M 119 S205A 120 Q209S 121 V212E 122 R213W 123 I216T 124 P217H 125 P217V 126 D219F 127 E220V 128 R221E 129 F223C 130 S226P 131 L228F 132 V234A 133 S238S 134 V236T 135 V236T 136 T241R 137 L242F 138 T241R 139 T241C 140 E248F 141 D250E 142 K257V 143 G256K 144 E260G 145 L262R 146 K263M 147 D267G 148 D267R 149 I265L 150 E268S 151 L270S 152 L270G 153 L270W 154 R271S 155 I274W 156 G278S 157 Y279C 158 S284R 159 E282G 160 T280N 161 V286G 162 R285F 163 V286R 164 G240G 165 E61R 166 E61D 167 E61Y 168 G85G 169 G85D 170 S80R 171 Y79R 172 Y79V 173 W283V 174 W283E 175 W283A 176 W283S 177 W283G 178 W283A 179 W283R 180 W283T 181 P281W  182* V236T; T241R *Mutant 182 was created using site-directed mutagenesis, using Mutant 136 DNA as a template and then introducing the V236T mutation. One skilled in the art can synthesize this gene using site-directed mutagenesis techniques.

Samples listed in Table 43 were then prepared for the tertiary screen. Glycerol stocks were used to inoculate 5 mL of LB containing 100 μg/mL carbenicillin. Cultures were grown overnight at 37° C. The overnight cultures were then used to inoculate 50 mL cultures of LB containing 100 μg/mL carbenicillin in 250 mL baffled flasks to OD_(600nm) of 0.05. IPTG was added to a final concentration of 1 mM when the OD_(600nm) reached 0.4-0.8. Cultures were induced overnight at 30° C. Cell pellets were harvested by centrifugation at 6,000 rpm for 20 minutes. Cell pellets were frozen at −20° C. until cell lysates could be prepared. Cells were lysed with BPER II (Thermo Scientific, Rockford, Ill.) on ice for 1 hour. Clarified lysates were prepared by centrifugation at 12,000 rpm for 30 minutes.

Protein was quantified by Bio-Rad Bradford Protein Assay (Bio-Rad, Hercules, Calif.) per the manufacturer's instructions. SDS-PAGE analysis and densitometry were used to determine the amount of expressed D-aminotransferase. Samples were normalized for expressed D-aminotransferase. 0.02 mg/mL D-aminotransferase was tested in the tertiary screen. The tertiary screening method was the same as the secondary screening method except that samples were taken at 0, 5, 10, 15, 30, 60, 120 and 210 minutes to develop a timecourse. Alanine production and monatin consumption values were measured by LC/MS/MS analysis and compared to a standard curve. Samples were compared to the wild type control.

Samples with higher final titers or faster initial rates than the wild type control were identified as hits and are referred to as upmutants. The GSSM^(SM) upmutants identified in the tertiary screen are listed in Table 44. These upmutants are further described in Example 27 below.

Example 27 Enzymatic Activity of Polypeptides Upmutants

This example describes data demonstrating the enzymatic activity of exemplary upmutant polypeptides disclosed herein, e.g., the polypeptides having amino acid sequences described in Table 44. Table 44 shows the activity of the upmutants relative to the wild type control at the 15 minute time point in reactions using 1 mM and 15 mM R,R-Monatin substrate. Relative activity is the amount of alanine produced by the sample divided by the amount of alanine produced by the wild type control.

TABLE 44 Activity of GSSM Upmutants in Tertiary Screen Activity relative to wild type control (SEQ ID NO: 220) Reaction with 1 mM Reaction with 15 mM Mutant Mutation monatin substrate monatin substrate 23 V42M 1.28 1.04 24 G41Y 1.37 1.31 27 V93G 1.73 1.98 31 P20S 1.29 1.60 35 V93L 1.14 0.96 40 N118G 2.61 1.52 44 N118R 1.55 0.47 45 N110A; N118G 2.50 2.02 46 N118A 2.28 0.69 48 P117W; N118K 2.54 1.12 58 L129V 1.04 0.85 66 F137W 1.25 1.44 67 I152V 1.19 1.24 81 C176G 1.11 1.27 82 A177S 1.24 1.02 104 I64H 1.37 1.07 109 T74L 1.37 1.31 110 K73L 2.83 3.75 111 T74V 1.99 2.19 112 T74M 1.78 2.01 135 V236T 3.44 2.88 136 T241R 2.64 1.79 152 L270G 1.24 0.89 153 L270W 2.00 1.54 174 W283E 1.23 0.84 175 W283A 1.61 1.09 177 W283G 1.71 1.06 6 Y6L 2.52 2.21 88 K185T 1.04 0.95 107 L66G 1.08 1.02

Several samples were identified that outperformed the wild type control under the conditions tested. Potential K_(m) and V_(max) upmutants were identified. These results indicate that the wild type control (SEQ ID NO:220) is further evolvable for increased specific D-aminotransferase activity on monatin.

Example 28 Activity of GSSM^(SM) Mutants in Monatin Process

Analysis of GSSM DATs in pSE420-C-His

This example describes data demonstrating the enzymatic activity of exemplary polypeptides disclosed herein. Mutant 27, Mutant 44, Mutant 58, Mutant 119, Mutant 135, Mutant 136, Mutant 152, Mutant 154 and the wild type control (in vector pSE420-C-His in E. coli XL1-Blue, as described in Examples 25 and 26) were streaked onto agar plates containing LB medium with ampicillin (100 μg/mL). Single colonies were used to inoculate 5 mL of LB medium containing ampicillin (100 μg/mL). Five hundred μl were used to inoculate 50 mL of the same medium in a 250 mL baffled flask. The cells were grown at 30° C. to approximately an OD_(600nm) of 0.4. IPTG was added to a final concentration of 1 mM. Cells were grown at 30° C. for 4 hours and collected by centrifugation. Cells were immediately frozen at −80° C. until cell extracts were prepared.

Cell extracts were prepared as described in Example 4. Protein concentrations were determined using the BCA (Pierce, Rockford, Ill.) microtiter plate assay with BSA (Pierce Rockford, Ill.) as the standard, per the manufacturer's instructions. To estimate the concentration of the D-aminotransferase in the cell-free extracts, SDS-PAGE analysis was done and visual estimation was used to estimate percentage of expression. The DAT proteins were soluble in the range of 10-25% expression as percentage of total protein and this was used to calculate the dosage of the assays.

An R,R monatin formation assay was performed containing 100 mM EPPS buffer pH 7.8, 1 mM MgCl², 0.05 mM PLP, 200 mM sodium pyruvate, 10 mM potassium phosphate, 0.01% Tween-80 with 0.1 mg/mL aldolase and 0.2 mg/mL of DAT in a 4 mL reaction at room temperature. Mutant 27 used 0.15 mg/mL of DAT enzyme instead of 0.2 mg/mL. After 0.5, 1, 2, 4 and 23 hours, an aliquot was taken, formic acid was added to a final concentration of 2%, and the samples spun and filtered. Samples were analyzed for monatin using the LC/MS/MS methodology described in Example 36. Results are shown in Table 45.

TABLE 45 Activity of DATs (cloned into pSE420-C-His) Monatin DAT Monatin (mM) Monatin (mM) Monatin (mM) (mM) polypeptide 0.5 hr 1 hr 2 hr 4 hr wild type 2.12 5.26 9.34 13.05 control Mutant 27 4.74 9.55 14.72 18.06 Mutant 44 3.73 6.61 10.38 13.23 Mutant 58 3.61 7.51 11.85 14.56 Mutant 135 3.50 7.72 12.50 16.17 Mutant 136 1.40 4.63 6.59 — Mutant 152 4.79 9.19 13.08 14.85 Mutant 154 3.76 7.66 11.85 14.38

As can be seen from the data shown in Table 45, a number of DAT mutants obtained through GSSM^(SM) evolution showed improved initial rates of monatin formation over the wild type control under the conditions of the assay.

Analysis of GSSM^(SM) DATs in pMet1a

This example describes data demonstrating the enzymatic activity of exemplary polypeptides disclosed herein. Mutant 2, Mutant 6, Mutant 11, Mutant 27, Mutant 40, Mutant 44, Mutant 45, Mutant 58, Mutant 110, Mutant 135, and Mutant 136 were recreated by site directed mutagenesis using QuikChange Multi Site-Directed Mutagenesis Kit (Stratagene, La Jolla, Calif.) according to the manufacturer's instructions. To generate the mutants, the pMET1a tagged construct described in Example 16 (pMET1a:SEQ ID NO:220(WT)) was used as the template. The mutagenic primers used are listed below in Table 46. The PCR fragments were digested with Dpn1 (Invitrogen, Carlsbad, Calif.) for 1 hour and transformed into E. coli Top 10 cells (Invitrogen, Carlsbad, Calif.). The resultant purified plasmid preparations were sequenced (Agencourt, Beverly, Mass.) to verify that the correct mutations were incorporated. The plasmids were then transformed into E. coli B834(DE3) expression host (Novagen, San Diego, Calif.).

TABLE 46 Primers for Mutagenesis Mutant produced PCR primers Template Mutant 2 5′-ATG GAC GCA CTG GGA TGT TAC AAC GGA AAT TGG-3′ SEQ ID (SEQ ID NO: 1084) NO: 220 5′-CCA ATT TCC GTT GTA ACA TCC CAG TGC GTC CAT-3′ (SEQ ID NO: 1085) Mutant 6 5′-ATG GAC GCA CTG GGA CTT TAC AAC GGA AAT TGG SEQ ID GGG-3′ (SEQ ID NO: 1086) NO: 220 5′-CCC CCA ATT TCC GTT GTA AAG TCC CAG TGC GTC CAT-3′ (SEQ ID NO: 1087) Mutant 5′-TAC CTG GTT TAT TGG CAG GGT ACT CGC GGA ACA SEQ ID 27 GGC CGG-3′ (SEQ ID NO: 1088) NO: 220 5′-CCG GCC TGT TCC GCG AGT ACC CTG CCA ATA AAC CAG GTA-3′ (SEQ ID NO: 1089) Mutant 5′-CTC TGG ATT ATA ATT AAG CCC GGC CAC ATC GAC SEQ ID 40 AAT CTT TAT AG-3′ (SEQ ID NO: 1090) NO: 220 5′-CTA TAA AGA TTG TCG ATG TGG CCG GGC TTA ATT ATA ATC CAG AG-3′ (SEQ ID NO: 1091) Mutant 5′-CTC TGG ATT ATA ATT AAG CCC AGG CAC ATC GAC SEQ ID 44 AAT CTT TAT AG-3′ (SEQ ID NO: 1092) NO: 220 5′-CTA TAA AGA TTG TCG ATG TGC CTG GGC TTA ATT ATA ATC CAG AG-3′ (SEQ ID NO: 1093) Mutant 5′-GTA TTT CCG GCA GGC CCT TCA GCG CTC TGG ATT ATA Mutant 40 45 ATT AAG CC-3′ (SEQ ID NO: 1094) 5′-GGC TTA ATT ATA ATC CAG AGC GCT GAA GGG CCT GCC GGA AAT AC-3′ (SEQ ID NO: 1095) Mutant 5′-CAA TCT TTA TAG AAA AAT CAA GGT TAT TAC CAT SEQ ID 58 GGA TGA TAC CCG C 3′ (SEQ ID NO: 1096) NO: 220 5′-GCG GGT ATG ATC CAT GGT AAT AAC CTT GAT TTT TCT ATA AAG ATT G-3′ (SEQ ID NO: 1097) Mutant 5′-CTT AAC AAA AGA GGA ATT GAA ACT GAC TTT AAA SEQ ID 110 TGA AAT GTA CTC C-3′ (SEQ ID NO: 1098) NO: 220 5′-GGA GTA CAT TTC ATT TAA AGT CAG TTT CAA TTC CTC TTT TGT TAA G-3′ (SEQ ID NO: 1099) Mutant 5′-TTC GAC GCG GAC GAG GTG CTT ACT TCC AGC AGC SEQ ID 135 GGC ACA CTC G-3′ (SEQ ID NO: 1100) NO: 220 5′-CGA GTG TGC CGC TGC TGG AAG TAA GCA CCT CGT CCG CGT CGA A-3′ (SEQ ID NO: 1101) Mutant 5′-TGC TTG TGT CCA GCA GCG GCC GGC TCG GCC TTA SEQ ID 136 GCG CCG-3′ (SEQ ID NO: 1102) NO: 220 5′-CGG CGC TAA GGC CGA GCC GGC CGC TGC TGG ACA CAA GCA-3′ (SEQ ID NO: 1103)

Mutant 2, Mutant 6, Mutant 27, Mutant 40, Mutant 45, Mutant 58, Mutant 110, Mutant 119, Mutant 131, Mutant 135, Mutant 136, Mutant 152, Mutant 154 were generated in the pMET1a vector and transformed into the compatible E. coli expression host B834(DE3) (Novagen, San Diego, Calif.) described in Example 2. Overnight cultures in LB medium containing carbenicillin (100 μg/mL) were diluted 1:100 in 100 mL of the same medium and grown in a 500 mL baffled flask. The culture was grown at 30° C. overnight to an OD_(600nm) of 10 in Overnight Express II medium (Solution 1-6, Novagen). Samples for total protein were taken immediately prior to harvesting. Cells were harvested by centrifugation and washed once with 10 mL of potassium phosphate buffer pH 7.8. Cells were immediately frozen at −80° C. until cell extracts were prepared. It is noted that, in addition to site-directed mutagenesis, one skilled in the art can synthesize the genes encoding these D-aminotransferases using multi-change mutagenesis PCR techniques such as those described in Example 25.

Cell extracts were prepared and desalted as described in Example 4 using 100 mM potassium phosphate as the buffer to elute and equilibrate the PD10 column. Total protein and DAT concentrations were determined as described.

Transamination of R,R monatin with pyruvate as the amino acceptor were performed as described in Example 5 except that 15 mM R,R monatin was utilized. Initial analyses of alanine, monatin, and monatin precursor levels identified Mutant 40, Mutant 135 and Mutant 136 as superior mutants resulting in the highest levels of alanine production as shown in Table 47. DAT Mutant 136 appeared to have the highest activity for conversion of R,R monatin to R-MP. The alanine production numbers (in mM) for the various time points are shown in Table 47.

TABLE 47 Alanine formation (mM) from R,R monatin transamination reactions from DATs cloned into pMET1a Alanine Alanine Alanine DAT (mM) Alanine (mM) (mM) (mM) polypeptide 15 minutes 30 minutes 60 minutes 120 minutes wild type 3.08 5.47 8.19 10.07 control Mutant 2 3.38 5.74 8.85 10.52 Mutant 6 3.51 5.97 8.99 10.81 Mutant 27 4.36 8.00 10.72 10.52 Mutant 40 7.89 10.37 11.79 12.50 Mutant 44 2.65 4.58 7.18 — Mutant 58 3.90 6.95 9.93 10.52 Mutant 110 3.50 6.17 9.53 10.52 Mutant 135 5.35 8.64 10.82 10.91 Mutant 136 6.24 9.46 11.24 11.15 Mutant 152 4.26 7.12 9.83 10.32 Mutant 154 4.16 7.13 10.07 10.76 —: not determined under present conditions

To further assess activity, a monatin formation assay was done as described in Example 1 with a DAT concentration of approximately 0.2 mg/mL. As a control, 0.2 mg/mL concentration of purified wild type DAT was evaluated. After 0.5, 1, 2, and 4 hrs, an aliquot was taken and formic acid was added to a final concentration of 2%, and the samples were spun and filtered. Samples were analyzed for monatin using the LC/MS/MS methodology described herein and for tryptophan and alanine using the LC/OPA post-column fluorescence methodology described in Example 36.

TABLE 48 Activity of DATs in pMET1a Monatin DAT Monatin (mM) Monatin (mM) Monatin (mM) (mM) polypeptide 0.50 hr 1.00 hr 2.00 hr 4.00 hr wild type 3.96 7.83 9.70 11.18 control Mutant 2 1.56 3.78 8.77 12.68 Mutant 27 4.70 9.70 n.d. 13.80 Mutant 44 3.03 5.61 8.50 12.28 Mutant 45 1.40 4.00 7.70 11.50 Mutant 58 3.83 7.23 11.33 14.12 Mutant 110 2.60 5.90 9.90 12.70 Mutant 119 4.12 7.87 11.37 13.50 Mutant 131 3.75 7.41 11.40 13.90 Mutant 135 6.39 10.65 13.49 13.15 Mutant 136 3.36 8.02 12.86 13.16 Mutant 154 3.00 6.06 10.67 13.17

All the DATs shown in Table 48 produced monatin. DAT mutants Mutant 58, Mutant 135 and Mutant 136 had faster initial rates than the wild type control. Mutant 136 was slower for reaction one (conversion of D-Trp to I3P) but had better overall monatin production than the wild type control.

For the final time point, an additional aliquot was taken (without the addition of formic acid) to determine the stereoisomeric distribution of the monatin produced using the FDAA derivatization methodology described in Example 36. For the select mutants tested, there was little to no impact on stereopurity. In all cases, the mutants produced over 98.8% R,R under the assay conditions tested. These results are shown in Table 49.

TABLE 49 Stereopurities of Monatin Produced by Select Mutants at 4 hours DAT polypeptide % SS % RS % RR % SR wild type (pMet1a) 0.00 0.40 99.30 0.20 control Mutant 6 0.00 0.40 99.50 0.10 Mutant 27 0.00 0.80 98.80 0.30 Mutant 40 0.00 0.20 99.80 0.00 Mutant 45 0.00 0.50 99.40 0.10 Mutant 110 0.10 0.40 99.30 0.10 Mutant 135 0.00 0.40 99.50 0.10 Mutant 136 0.02 1.00 99.00 0.03

Example 29 Construction and Testing of Tailored Multi-Site Combinatorial Assembly (TMCA^(SM)) Mutants

This example describes the construction of exemplary nucleic acids and polypeptides, and describes their enzymatic activity. A subset of GSSM mutations were selected for combination using Tailored Multi-Site Combinatorial Assembly^(SM) (TMCA^(SM)) technology. The top ten performers from the GSSM evolution in either the 1 or 15 mM monatin reactions were selected for TMCA^(SM) evolution. The wild type sequence (SEQ ID NO:220) was threaded onto a model of 3DAA-D amino acid aminotransferase (FIG. 4). The model in FIG. 4 is shown with pyridoxyl-5′-phosphate D-alanine, with the numbered residues indicating those sites selected for TMCA^(SM) evolution. Table 50 also lists the mutations that were selected for inclusion in the TMCA^(SM) library. TMCA^(SM) evolution was performed on wild type (SEQ ID NO:220) and Mutant 45 using the methods described in PCT Application No. PCT/US08/071,771.

TMCA evolution is described in PCT Application Number PCT/US08/071,771 and comprises a method for producing a plurality of progeny polynucleotides having different combinations of various mutations at multiple sites. The method can be performed, in part, by a combination of at least one or more of the following steps:

Obtaining sequence information of a (“first” or “template”) polynucleotide. For example, the first or template sequence can be a wild type (e.g. SEQ ID NO:220) or mutated (e.g. Mutant 45) sequence. The sequence information can be of the complete polynucleotide (e.g., a gene or an open reading frame) or of partial regions of interest, such as a sequence encoding a site for binding, binding-specificity, catalysis, or substrate-specificity.

Identifying three or more mutations of interest along the first or template polynucleotide sequence. For example, mutations can be at 3, 4, 5, 6, 8, 10, 12, 20 or more positions within the first or template sequence. The positions can be predetermined by absolute position or by the context of surrounding residues or homology. For TMCA of DAT polypeptides, the top 10 codon changes that resulted in improved enzyme performance were included as mutations of interest. The sequences flanking the mutation positions on either side can be known. Each mutation position may contain two or more mutations, such as for different amino acids. Such mutations can be identified by using Gene Site Saturation Mutagenesis^(SM) (GSSM^(SM)) technology, as described herein and in U.S. Pat. Nos. 6,171,820; 6,562,594; and 6,764,835.

Providing primers (e.g., synthetic oligonucleotides) comprising the mutations of interest. In one embodiment, a primer is provided for each mutation of interest. Thus, a first or template polynucleotide having 3 mutations of interest can use 3 primers at that position. The primer also can be provided as a pool of primers containing a degenerate position so that the mutation of interest is the range of any nucleotide or naturally occurring amino acid, or a subset of that range. For example, a pool of primers can be provided that favor mutations for aliphatic amino acid residues.

The primers can be prepared as forward or reverse primers, or the primers can be prepared as at least one forward primer and at least one reverse primer. When mutations are positioned closely together, it can be convenient to use primers that contain mutations for more than one position or different combinations of mutations at multiple positions.

Providing a polynucleotide containing the template sequence. The first or template polynucleotide can be circular, or can be supercoiled, such as a plasmid or vector for cloning, sequencing or expression. The polynucleotide may be single-stranded (“ssDNA”), or can be double-stranded (“dsDNA”). For example, the TCMA method subjects the supercoiled (“sc”) dsDNA template to a heating step at 95° C. for 1 min (see Levy, Nucleic Acid Res., 28(12):e57(i-vii) (2000)).

Adding the primers to the template polynucleotide in a reaction mixture. The primers and the template polynucleotide are combined under conditions that allow the primers to anneal to the template polynucleotide. In one embodiment of the TMCA protocol, the primers are added to the polynucleotide in a single reaction mixture, but can be added in multiple reactions.

Performing a polymerase extension reaction. The extension products (e.g., as a “progeny” or “modified extended polynucleotide”) may be amplified by conventional means. The products may be analyzed for length, sequence, desired nucleic acid properties, or expressed as polypeptides. Other analysis methods include in-situ hybridization, sequence screening or expression screening. The analysis can include one or more rounds of screening and selecting for a desired property.

The products can also be transformed into a cell or other expression system, such as a cell-free system. The cell-free system may contain enzymes related to DNA replication, repair, recombination, transcription, or for translation. Exemplary hosts include bacterial, yeast, plant and animal cells and cell lines, and include E. coli, Pseudomonas fluorescens, Pichia pastoris and Aspergillus niger. For example, XL1-Blue or Stb12 strains of E. coli can be used as hosts.

The method of the invention may be used with the same or different primers under different reaction conditions to promote products having different combinations or numbers of mutations.

By performing the exemplary method described above, this protocol also provides one or more polynucleotides produced by this TMCA evolution method, which then can be screened or selected for a desired property. One or more of the progeny polynucleotides can be expressed as polypeptides, and optionally screened or selected for a desired property. Thus, this embodiment of the TMCA evolution protocol provides polynucleotides and the encoded polypeptides, as well as libraries of such polynucleotides encoding such polypeptides. This embodiment of the TMCA evolution protocol further provides for screening the libraries by screening or selecting the library to obtain one or more polynucleotides encoding one or more polypeptides having the desired activity.

Another embodiment of the TMCA evolution protocol described in PCT/US08/071,771 comprises a method of producing a plurality of modified polynucleotides. Such methods generally include (a) adding at least three primers to a double stranded template polynucleotide in a single reaction mixture, wherein the at least three primers are not overlapping, and wherein each of the at least three primers comprise at least one mutation different from the other primers, wherein at least one primer is a forward primer that can anneal to a minus strand of the template and at least one primer is a reverse primer that can anneal to a plus strand of the template, and (b) subjecting the reaction mixture to a polymerase extension reaction to yield a plurality of extended modified polynucleotides from the at least three primers.

Another embodiment of the TMCA evolution protocol described in PCT/US08/071,771 comprises a method wherein a cell is transformed with the plurality of extended products that have not been treated with a ligase. In another embodiment of the invention, the plurality of extended modified polynucleotides is recovered from the cell. In another embodiment, the recovered plurality of extended modified polynucleotides is analyzed, for example, by expressing at least one of the plurality of extended modified polynucleotides and analyzing the polypeptide expressed therefrom. In another embodiment, the plurality of extended modified polynucleotides comprising the mutations of interest is selected.

In another embodiment of the TMCA evolution protocol, sequence information regarding the template polynucleotide is obtained, and three or more mutations of interest along the template polynucleotide can be identified. In another embodiment, products obtained by the polymerase extension can be analyzed before transforming the plurality of extended modified products into a cell.

In one embodiment of the TMCA evolution protocol, products obtained by the polymerase extension are treated with an enzyme, e.g., a restriction enzyme, such as a Dpn1 restriction enzyme, thereby destroying the template polynucleotide sequence. The treated products can be transformed into a cell, e.g., an E. coli cell.

In one embodiment of the TMCA evolution protocol, at least two, or at least three, or at least four, or at least five, or at least six, or at least seven, or at least eight, or at least nine, or at least ten, or at least eleven, or at least twelve, or more primers can be used. In one embodiment, each primer comprises a single point mutation. In another embodiment, two forward or two reverse primers comprise a different change in the same position on the template polynucleotide. In another embodiment, at least one primer comprises at least two changes in different positions on the template polynucleotide. In yet another embodiment, at least one primer comprises at least two changes in different positions and at least two forward or two reverse primers comprise a different change in the same position on the template polynucleotide.

In one embodiment of the TMCA evolution protocol, the forward primers are grouped into a forward group and the reverse primers are grouped into a reverse group, and the primers in the forward group and the primers in the reverse group, independent of one another, are normalized to be equal concentration in the corresponding group regardless of positions on the template polynucleotide, and wherein after the normalization an equal amount of the forward and reverse primers is added to the reaction. In this normalization method, a combination of some positions may be biased. The bias can be due to, for example, a relatively low primer concentration at one position containing a single primer compared to a position containing multiple primers. “Positional bias” refers to resulting polynucleotides which show a strong preference for the incorporation of primers at a single position relative to the other positions within its forward or reverse primer group. This results in a combination of modified polynucleotides which may have a high percentage of mutations within a single primer position but a low percentage of mutations at another position within its forward or reverse primer group. This bias is unfavorable when the goal of the TMCA is to generate progeny polynucleotides comprising all possible combinations of changes to the template. The bias can be corrected, for example, by normalizing the primers as a pool at each position to be equal.

In one embodiment of the TMCA evolution protocol, the primer normalization is performed by organizing the primers into multiple groups depending on their location on the template polynucleotide, wherein the primers covering the same selected region on the template are in one group; normalizing the grouped primers within each group to be equal concentration; pooling the forward primers within one group into a forward group and normalizing concentration between each group of the forward primers to be equal; pooling the reverse primers within one group into a reverse group and normalizing concentration between each group of the reverse primers to be equal; and adding an equal amount of the pooled forward and reversed primers into the reaction. No bias has been observed for position combinations.

In one embodiment of the TMCA evolution protocol, a set of degenerate primers each comprising a degenerate position is provided, wherein the mutation of interest is a range of different nucleotides at the degenerate position. In another embodiment, a set of degenerate primers is provided comprising at least one degenerate codon corresponding to at least one codon of the template polynucleotide and at least one adjacent sequence that is homologous to a sequence adjacent to the codon of the template polynucleotide sequence. In another embodiment, the degenerated codon is N,N,N and encodes any of 20 naturally occurring amino acids. In another embodiment, the degenerated codon encodes less than 20 naturally occurring amino acids.

Another embodiment of the TMCA evolution protocol described in PCT/US08/071,771 comprises a method of producing a plurality of modified polynucleotides comprising the mutations of interest. Such methods generally include (a) adding at least two primers to a double stranded template polynucleotide in a single reaction mixture, wherein the at least two primers are not overlapping, and wherein each of the at least two primers comprise at least one mutation different from the other primer(s), wherein at least one primer is a forward primer that can anneal to a minus strand of the template and at least one primer is a reverse primer that can anneal to a plus strand of the template, (b) subjecting the reaction mixture to a polymerase extension reaction to yield a plurality of extended modified polynucleotides from the at least two primers, (c) treating the plurality of extended modified polynucleotides with an enzyme, thereby destroying the template polynucleotide, (d) transforming the treated extended modified polynucleotides that have not been treated with a ligase into a cell, (e) recovering the plurality of extended modified polynucleotides from the cell, and (f) selecting the plurality of extended modified polynucleotides comprising the mutations of interest.

TABLE 50 List of Sites for TMCA evolution Mutation New Codon P20S AGT K73L TTG T74V GTG V93G GGT N110A GCT P117W TGG N118G GGG N118A GCG V236T ACT T241R CGG L270W TGG

TMCA mutants were grown, arrayed, assayed and sequenced using the same method as described for the GSSM evolution in Example 25. Sample performance was compared to the performance of the top candidate from GSSM evolution—Mutant 135—using the same scoring system as described in Example 25. Table 52 lists the TMCA secondary screen hits with unique DNA sequences (TMCA mutants are designated with alphabetic characters to distinguish them from GSSM mutants, which are designated numerically).

TABLE 52 TMCA Mutants Identified as Secondary Screen Hits Mutant name Mutation A P20S-N118G B T74V-V93G-L270W C P20S-T74V-L270W D T74V-L270W E P20S-K73L-T241R-L270W F K73L-V93G-V236T-T241R G P20S-T74V-V236T H P20S-K73L-V93G I K73L-V236T J P20S-L270W K 2N-P20S-K73L-V93G-N118G L P20S-T74V-N118A M P20S-V236T N P20S-T241R-L270W O P20S-T241R P T74V-V93G-V236T-T241R Q P20S-K73L-T74V-L270W R P20S-V93G-V236T S P20S-K73L-T74V-T241R-L270W T P20S-K73L-L270W U T74V-V93G-N118G-V236T-T241R V P20S-K73L-210A (SILENT-GCC → GCT)-V236T W N118A-L270W X P20S-58K (SILENT AAG → AAA)-L270W Y P20S-V93G-N118G Z P20S-V236T-T241R AA P20S-P117W-N118A-V236T- L270W BB V93G-V236T CC V236T-L270W DD P20S-N118G-L270W EE P20S-N110A-N118G FF N110A-N118G-T241R-L270W GG P20S-T74V-V93G-N110A-N118G- V236T-L270W HH V93G-N110A-N118G-V236T II P20S-T74V-N110A-N118G JJ N110A-N118G KK P20S-V93G-N110A-N118G-T241R LL N110A-N118A-L270W MM P20S-N110A-N118G-L270W NN N110A-N118A-V236T-T241R OO N110A-N118G-L270W PP V93G-N110A-N118G-T241R QQ P20S-V93G-N110A-N118G RR V93G-N110A SS P20S-N110A-N118G-V236T TT T74V-N110A-N118A-V236T UU P20S-K73L-T74V-N110A-N118G- V236T-T241R VV 86E (SILENT GAG → GAA)- N110A-N118A-V236T WW T74V-N118G XX P20S-T241R-L270W-277T (SILENT ACA → ACG) YY T74V-N118A-L270W ZZ P20S-K73L-N118A-L270W AAA P20S-V93G-T241R BBB T74V-V93G-N110A-T241R CCC V93G-N110A-N118A DDD P20S-T74V-V93G-N110A-N118G- T241R EEE T74V-N110A-N118G-L270W FFF P20S-231A (SILENT GCG → GCA)-V236T GGG V93G-V236T-T241R

The samples identified in Table 52 were grown, normalized and assayed in the tertiary screen using the same method as described for the GSSM evolution in Example 26. Monatin and alanine values were determined by LC/MS/MS and compared to a standard curve. Sample performance was compared to the activity of Mutant 135 (the top performer from GSSM evolution). TMCA upmutants identified in the tertiary screen are listed in Table 53.

Example 31 Activity of TMCA Hits

This example describes data demonstrating the enzymatic activity of exemplary polypeptides. Table 53 below shows the activity of the upmutants relative to Mutant 135 at the minute time point in reactions using 1 mM and 15 mM R,R-monatin substrate. Relative activity is the amount of alanine produced by the sample divided by the amount of alanine produced by Mutant 135.

TABLE 53 Activity of TMCA Upmutants in Tertiary Screen Activity relative to GSSM Mutant 135 Reactions Reactions with 1 mM with 15 mM monatin monatin Mutant Mutation substrate substrate C P20S-T74V-L270W 1.02 0.93 E P20S-K73L-T241R-L270W 1.32 1.31 F K73L-V93G-V236T-T241R 1.29 0.64 G P20S-T74V-V236T 1.28 1.30 I K73L-V236T 1.24 1.29 J P20S-L270W 0.79 1.01 L P20S-T74V-N118A 1.62 0.83 M P20S-V236T 1.27 1.46 O P20S-T241R 1.33 1.71 R P20S-V93G-V236T 1.22 1.02 S P20S-K73L-T74V-T241R- 1.16 1.18 L270W V P20S-K73L-210A 1.03 1.00 (SILENT-GCC → GCT)- V236T Z P20S-V236T-T241R 1.02 0.89 BB V93G-V236T 1.55 1.98 CC V236T-L270W 1.24 1.40 DD P20S-N118G-L270W 1.54 1.78 PP V93G-N110A-N118G-T241R 1.40 1.53 TT T74V-N110A-N118A-V236T 1.10 0.42 VV 86E (SILENT GAG → 1.31 0.52 GAA)-N110A-N118A-V236T WW T74V-N118G 1.23 1.49 YY T74V-N118A-L270W 1.97 1.30 ZZ P20S-K73L-N118A-L270W 1.01 0.44 AAA P20S-V93G-T241R 1.86 3.49 CCC V93G-N110A-N118A 1.26 0.56

Several samples were identified that outperformed Mutant 135 under the conditions tested. Potential K_(m) and V_(max) upmutants were identified. The results of the GSSM and TMCA evolutions indicate that wild type SEQ ID NO:220 is further evolvable for increased specific activity on monatin.

Example 32 Evaluation of TMCA Mutant DATs in pMET1a

This example describes data demonstrating the enzymatic activity of exemplary polypeptides disclosed herein. Mutant E, Mutant G, Mutant I, Mutant M, Mutant O, Mutant P, Mutant BB, Mutant PP, Mutant WW, and Mutant AAA (DATs created using TMCA technology, see Examples 29 and 30) were recreated by site-directed mutagenesis using QuikChange Multi Site-Directed Mutagenesis Kit (Stratagene, La Jolla, Calif.) according to the manufacturer's instructions. To generate the mutants, pMET1a tagged constructs described in Example 16 and Example 28 were used as templates. The mutagenic primers used are listed below in Table 54. The PCR fragments were digested with Dpn1 (Invitrogen, Carlsbad, Calif.) for 1 hour and transformed into E. coli XL10-Gold cells (Stratagene, La Jolla, Calif.). The resultant purified plasmid preparations were sequenced (Agencourt, Beverly, Mass.) to verify that the correct mutations were incorporated. The plasmids were then transformed into E. coli B834(DE3) expression host (Novagen, San Diego, Calif.).

TABLE 54 Primers for Mutants in pMET1a Vector TMCA mutant polypeptide produced PCR primers Template Mutant E 5′-CTG GAC GAG ATG ACT GTG AGT ATG AAC GAC AGG Mutant GGC TGC TAC-3′ (SEQ ID NO: 1104) 110 5′-TGC TTG TGT CCA GCA GCG GCC GGC TCG GCC TTA GCG CCG-3′ (SEQ ID NO: 1105) 5′CTA AAA AAA ATC CAG GAT GAA GTG TGG AGG GAA TTT ATC GAA GCG ACA GG3′ (SEQ ID NO: 1106) Mutant G 5′-CAA AAG AGG AAT TGA AAA AAG TGT TAA ATG AAA Mutant M TGT ACT CC-3′ (SEQ ID NO: 1107) 5′-GGA GTA CAT TTC ATT TAA CAC TTT TTT CAA TTC CTC TTT TG-3′ (SEQ ID NO: 1108) Mutant I 5′-CTT AAC AAA AGA GGA ATT GAA ACT GAC TTT AAA Mutant TGA AAT GTA CTC C-3′ (SEQ ID NO: 1109) 135 5′-GGA GTA CAT TTC ATT TAA AGT CAG TTT CAA TTC CTC TTT TGT TAA G-3′ (SEQ ID NO: 1110) Mutant M 5′-CTG GAC GAG ATG ACT GTG AGT ATG AAC GAC AGG Mutant GGC TGC TAC-3′ (SEQ ID NO: 1111) 135 5′-GTA GCA GCC CCT GTC GTT CAT ACT CAC AGT CAT CTC GTC CAG-3′ (SEQ ID NO: 1112) Mutant O 5′-CTG GAC GAG ATG ACT GTG AGT ATG AAC GAC AGG Mutant GGC TGC TAC-3′ (SEQ ID NO: 1113) 136 5′-GTA GCA GCC CCT GTC GTT CAT ACT CAC AGT CAT CTC GTC CAG-3′ (SEQ ID NO: 1114) Mutant P 5′-CAA AAG AGG AAT TGA AAA AAG TGT TAA ATG AAA Mutant TGT ACT CC-3′ (SEQ ID NO: 1115) 27 5′-TTC GAC GCG GAC GAG GTG CTT ACT TCC AGC AGC GGC ACA CTC G-3′ (SEQ ID NO: 1116) 5′-TGC TTG TGT CCA GCA GCG GCC GGC TCG GCC TTA GCG CCG-3′ (SEQ ID NO: 1117) Mutant BB 5′-TAC CTG GTT TAT TGG CAG GGT ACT CGC GGA ACA Mutant GGC CGG-3′ (SEQ ID NO: 1118) 135 5′-CCG GCC TGT TCC GCG AGT ACC CTG CCA ATA AAC CAG GTA-3′ (SEQ ID NO: 1119) Mutant PP 5′-TAC CTG GTT TAT TGG CAG GGT ACT CGC GGA ACA Mutant GGC CGG-3′ (SEQ ID NO: 1120) 45 5′-TGC TTG TGT CCA GCA GCG GCC GGC TCG GCC TTA GCG CCG-3′ (SEQ ID NO: 1121) Mutant 5′-CAA AAG AGG AAT TGA AAA AAG TGT TAA ATG AAA Mutant M WW TGT ACT CC-3′ (SEQ ID NO: 1122) 5′-GGA GTA CAT TTC ATT TAA CAC TTT TTT CAA TTC CTC TTT TG-3′ (SEQ ID NO: 1123) Mutant 5′-CTG GAC GAG ATG ACT GTG AGT ATG AAC GAC AGG Mutant AAA GGC TGC TAC-3′ (SEQ ID NO: 1124) 27 5′-TGC TTG TGT CCA GCA GCG GCC GGC TCG GCC TTA GCG CCG-3′ (SEQ ID NO: 1125)

E. coli B834(DE3) (Novagen, San Diego, Calif.) cultures expressing carboxy-terminal His-tagged Mutant 110, Mutant 135, Mutant 136, Mutant E, Mutant G, Mutant I, Mutant M, Mutant O, Mutant P, Mutant BB, Mutant PP, Mutant WW, Mutant AAA and wild type (SEQ ID NO:220) proteins were grown in 200 mL of Overnight Express II medium (Solution 1-6, Novagen) in a 500 mL baffled flask overnight at 30° C. to an OD₆₀₀ of 10. Samples for total protein were taken immediately prior to harvesting. Cells were harvested by centrifugation and immediately frozen at −80° C. until cell extracts were prepared as described in Example 4.

Cell extracts were created by the addition of 50 mL of Bug Buster Primary Amine Free (Novagen, San Diego, Calif.) with 50 μl of Benzonase Nuclease (Novagen, San Diego, Calif.), 0.75 μl of rLysozyme (Novagen, San Diego, Calif.), and 250 μl of Protease Inhibitor Cocktail II (Calbiochem, San Diego, Calif.). The cells were incubated for 15 minutes at room temperature with gentle rocking. The extracts were centrifuged at 45,000×g for 10 minutes.

His-tagged proteins were purified as described in Example 4 using GE Healthcare (Piscataway, N.J.) Chelating Sepharose™ Fast Flow resin. The exception was Mutant 182, which was analyzed as CFE as described in Example 4. Purified protein was desalted using a PD10 column into 100 mM potassium phosphate, pH 7.8 with 0.050 mM PLP. Total protein and DAT concentrations were determined as described in Example 4.

A 3-step monatin formation assay was done as described in Example 5 with a DAT concentration of approximately 0.2 mg/mL and the aldolase at a concentration of 0.1 mg/mL. As a control, 0.2 mg/mL concentration of purified wild type DAT (SEQ ID NO:220) was evaluated. After 0.5, 1, 2, 4 and 24 hours, an aliquot was taken, formic acid was added to a final concentration of 2% and the samples were spun and filtered. Samples were analyzed for monatin using LC/MS/MS methodology and for tryptophan and alanine using the LC/OPA post-column fluorescence methodology described in Example 36. At the last time point, an additional aliquot was taken (without pH adjustment) to determine % R,R monatin by the FDAA-derivatization method described in Example 36. The amount of monatin (mM) produced at various time points can be found in Table 55. Stereopurity was also determined and the percent of the R,R stereoisomer can be found in the far right hand column. The stereoisomer R,S made up the majority of the balance.

TABLE 55 Activity of Select DAT Mutants Monatin Monatin Monatin Monatin DAT (mM) (mM) (mM) (mM) polypeptide 0.25 hr 0.5 hr 1 hr 4 hr % RR Wild type 1.60 (±0.42) 2.95 (±0.64) 5.00 (±0.85) 11.40 (±0.42) 99.50 (±0.08) control (SEQ ID NO: 220) Mutant 110 1.70 (±0.00) 3.20 (±0.85) 5.75 (±0.21) 12.60 (±0.14) 99.48 (±0.32) Mutant 135 3.65 (±0.35) 6.17 (±0.65) 10.33 (±0.32)  13.20 (±0.56) 99.42 (±0.11) Mutant 136 2.60 5.00 8.10 12.90 98.98 Mutant 182 — 3.20 6.80 14.30 99.50 Mutant E 1.80 3.80 8.60 18.60 99.45 Mutant G 3.10 6.50 9.90 12.90 99.05 Mutant I 2.90 5.30 8.50 12.90 99.46 Mutant M 4.20 8.10 11.20 13.80 98.96 Mutant O 2.60 5.70 9.50 14.00 98.59 Mutant BB 4.20 8.20 11.40 13.70 98.97 Mutant PP 2.40 3.20 6.20 17.40 97.25 Mutant AAA 2.80 6.80 11.80 14.80 97.98 —= not determined under conditions tested

The relative rates of monatin production under the conditions tested indicate the greatest improvement in initial activity from Mutant 135, Mutant 136, Mutant E, Mutant G, Mutant M, Mutant O, Mutant BB, and Mutant AAA as determined by comparing the rate of monatin formation with purified protein over the first hour between the mutants and the wild type control (SEQ ID NO:220) DAT. DATs Mutant E and Mutant AAA had high activity but were not well expressed (less than 5% of the total protein) nor very soluble under the conditions tested.

The assay samples were also analyzed for intermediates such as monatin precursor, I3P, and byproduct 4-hydroxy-4-methyl glutmatic acid (HMG) as described in Example 36. The analysis of the amount of HMG formed was determined for the mutants Mutant E, Mutant G, Mutant 1, Mutant M, Mutant O, Mutant BB, Mutant PP, Mutant AAA and Mutant 110, Mutant 135, and Mutant 136. It appears that at the 4 hour time point, more HMG were formed by the mutants Mutant 135, Mutant G, Mutant 1, Mutant M and Mutant BB. These mutants all contained the change V236T. HMG was also present above the levels of the wild type control (SEQ ID NO:220) with mutants Mutant E, Mutant G, Mutant M and Mutant AAA likely due to the change in residue P20S.

TABLE 56 HMG Formation by DAT Mutants after 4 hours HMG (mM) DAT polypeptide 4 hr Wild type control nd (SEQ ID NO: 220) Mutant 110 nd Mutant 135 1.0 Mutant 136 nd Mutant E 0.2 Mutant G 1.6 Mutant I 0.8 Mutant M 1.6 Mutant O nd Mutant BB 1.5 Mutant AAA 0.6 nd = not detected DAT Assay Monitoring I3P Formation

The formation of I3P from tryptophan was detected and monitored at a wavelength of 340 nm. Reactions were carried out in 1 mL reaction volume containing 900 μL, of a 25 mM D-tryptophan, 25 mM pyruvic acid sodium salt, 0.05 mM PLP, 100 mM potassium phosphate (pH 7.8) solution combined with 100 μL dilutions of DAT (total protein) prepared as described above. Enzymes were diluted 1:100 and 1:200 with cold 50 mM potassium phosphate (pH 7.8) and 50 μM PLP prior to addition to the assay. Enzyme was added to the reaction mixture 1:100 and monitored in increments of 15 seconds for 3 minutes. The formation of indole-3-pyruvate (I3P) was monitored at a wavelength of 340 nm for 3 minutes on a BioRad Spectrophotometer (GE Healthscience, Piscataway, N.J.) and rates were measured within the dynamic range of a standard curve. The standard curve was generated with purified wild type (SEQ ID NO:220) DAT protein and the concentration of DAT in cell extract was determined based on the equation of the line for the standard curve. The effective concentration of DAT with respect to the wild type DAT for the first reaction is reported in Table 57.

TABLE 57 Activity of DAT (Conversion of Tryptophan to I3P) Concentration of Rate of 13P DAT formation (determined by Activity relative to DAT (Δ Abs340 nm/ activity) Wild type of first Polypeptide minute) mg/mL reaction Wild type 0.058 0.065 1.0 control (SEQ ID NO: 220) Mutant 135 0.067 0.075 1.2 Mutant 136 0.017 0.019 0.3 Mutant E 0.000 0.002 0.1 Mutant G 0.027 0.030 0.5 Mutant M 0.050 0.055 0.9 Mutant O 0.031 0.033 0.8 Mutant BB 0.045 0.050 0.8 Mutant AAA 0.002 0.004 0.2

The wild type DAT (SEQ ID NO:220) and mutants 136, E, G, M, O, BB and AAA can facilitate the conversion of both tryptophan to I3P and of monatin precursor to monatin. Table 57 shows that these mutants had lower activity for the conversion of tryptophan to I3P relative to the wild type DAT (SEQ ID NO:220). Yet, according to Table 55, the same mutants produced more total monatin from tryptophan than did the wild type DAT (SEQ ID NO:220). Thus, under the conditions of the assay described herein, there appears to be a beneficial effect on monatin production through controlling the conversion of tryptophan to I3P in the monatin biosynthetic pathway. For example, although Mutant E showed the lowest relative activity for conversion of tryptophan to I3P (see Table 57), it also produced the highest amount of monatin at 4 hours (see Table 55). Without being bound by theory, the beneficial effects of controlling the first step in the reaction could be attributed to a reduction of I3P buildup and subsequent potential I3P degradation to products other than monatin. Generally, it also appears that controlling the rate of one or more of the reactions involved in the production of monatin from tryptophan, using, for example, one or more mutant DATs, can have a beneficial effect on the total amount of monatin produced.

Example 33 Evaluation of Mutant DATs at 35° C.

This example describes data demonstrating the enzymatic activity of exemplary polypeptides disclosed herein. Starter cultures were grown overnight at 37° C. with shaking at 250 rpm until the OD_(600nm) reached 0.05. 200 mL of Overnight Express II medium (Novagen, San Diego, Calif.) was inoculated and grown as described in Example 3. Cultures were grown in duplicate and the cell pellets were combined. The pellets were resuspended in 40 mL of 50 mM sodium phosphate buffer (pH 7.8) with 0.05 mM PLP and lysed using a French Press (Sim Aminco, Rochester, N.Y.) per the manufacturer's instructions. The supernatant was collected in a clean tube and stored at −80° C. until used.

A 3-step monatin formation assay was performed as described in the methods with a DAT concentration of approximately 0.2 mg/mL and the aldolase at a concentration of 0.1 mg/mL in glass vials. Duplicate samples were incubated at either 25° C. or 35° C. and after 1, 3, and 4 hours, an aliquot was taken and formic acid was added to a final concentration of 2%, and the samples were spun and filtered. Samples were analyzed for monatin using LC/MS/MS methodology and for tryptophan and alanine using the LC/OPA post-column fluorescence methodology described in Example 36. Samples were also analyzed for intermediates such as monatin precursor, I3P, and 4-hydroxy-4-methyl glutmatic acid (HMG) as described in Example 36. The amount of monatin (mM) produced at various time points is shown in Table 58.

The monatin formation assay was repeated for the wild type control (SEQ ID NO:220), Mutant 135 and Mutant M under similar conditions except the reactions were carried out in plastic vials. Monatin production at various time points can be found in Table 58.

TABLE 58 Monatin Formation at 25° C. and 35° C. Monatin Monatin (mM) Monatin (mM) (mM) DAT polypeptide 1 hr 3 hr 4 hr 25° C. wild type control (pMet1a) 2.0 6.8 8.4 Mutant 135 (V236T) 10.0 14.4 14.2 Mutant 136 (241R) 4.0 10.8 12.4 Mutant E (20S, 73L, 241R, 270W) 0.8 4.2 5.6 Mutant M (20S, 236T) 10.0 13.4 14.2 Mutant O (20S, 241R) 8.0 13.8 13.6 Mutant BB (93G, 236T) 4.0 11.4 12.4 Mutant AAA (20S, 93G, 241R) 0.2 1.0 1.6 35° C. wild type control (pMet1a) 2.2 5.4 6.2 Mutant 135 (V236T) 9.4 9.2 9.8 Mutant 136 (241R) 4.8 9.4 10.4 Mutant E (20S, 73L, 241R, 270W) 0.6 3.4 4.2 Mutant M (20S, 236T) 9.2 13.6 14.6 Mutant O (20S, 241R) 9.6 10.6 10.8 Mutant BB (93G, 236T) 4.6 9.0 9.2 Mutant AAA (20S, 93G, 241R) 0.2 1.6 2.2

Lower monatin titers were observed using the DAT enzymes described here at 35° C. under the conditions of the assay. However, select mutants Mutant 135, Mutant 136, Mutant M, Mutant O and Mutant BB showed increased initial monatin production rates and greater 4 hour monatin titers than the wild type control (SEQ ID NO:220) at 35° C. under the assay conditions.

Example 34 Evaluation of Mutant DATs in BioReactors

This example describes data demonstrating the enzymatic activity of exemplary polypeptides disclosed herein in bioreactors. Glycerol stocks of the wild type control (SEQ ID NO:220), Mutant 135, Mutant 136, Mutant M, Mutant O, and Mutant BB were used to streak plates for single colonies. Single colonies were used to inoculate flasks containing 5 mL of LB medium with the appropriate antibiotic. The starter cultures were grown overnight at 37° C. with shaking at 250 rpm and the OD_(600nm) was checked. When the OD_(600nm) reached 0.05, the 5 mL culture was inoculated into a 200 mL of Overnight Express II medium (Novagen, San Diego, Calif.) and then incubated at 30° C. with shaking at 250 rpm. Each culture was grown in duplicate and the cell pellets were combined. Cultures were harvested by pelleting cells by centrifugation at 4000 rpm for 15 minutes. The supernatant was poured off and the pellet was either frozen for later use or resuspended in 40 mL of 50 mM sodium phosphate buffer (pH 7.8) and lysed using a French Press (Sim Aminco, Rochester, N.Y.) or a microfluidizer (Microfluidics Corporation, Newton, Mass.) per the manufacturer's instructions. The supernatant was collected in a clean tube and stored at −80° C. until used. Approximately 1 mL of the clarified lysate was retained for protein quantitation using the BCA assay (Pierce, Rockford, Ill.) and SDS-PAGE analysis.

Bench scale reactions (250 mL) were carried out in 0.7 L Sixfors agitated fermentors (Infors AG, Bottmingen, Switzerland) under a nitrogen headspace as described in Example 15. The reaction mix contained 10 mM potassium phosphate, 1 mM MgCl₂, 0.05 mM PLP, 200 mM sodium pyruvate and 130 mM D-tryptophan. The reaction mix was adjusted to 25° C. and adjusted to pH 7.8 with potassium hydroxide. The aldolase described in Example 6 was added as a clarified cell extract at 0.02 mg/mL of target protein. Wild type control (SEQ ID NO:220), Mutant 135, Mutant 136, Mutant M, Mutant O, and Mutant BB DATs have soluble protein expressions ranging from 15-35% based on visual estimation. The clarified cell extracts were added at 0.20 mg/mL of target protein.

The progress of the reactions was followed by measuring monatin production at 1, 2, 4 and 24 hours using the LC/MS/MS methodology described in Example 36. The results are shown in Table 59.

TABLE 59 Monatin Production in Fermentors Monatin Monatin Monatin Monatin Protein (mM) (mM) (mM) (mM) DAT polypeptide Expression 1 hr 2 hr 4 hr 24 hr wild type control 25% 0.90 2.80 12.40 12.80 Mutant 135 30% 0.50 8.80 12.40 12.40 Mutant 136 35% 3.80 7.80 11.60 12.80 Mutant M 15% 3.40 6.80 12.10 12.20 Mutant O 15% 5.20 8.60 10.90 9.80 Mutant BB 15% 3.40 6.20 10.50 12.60

The initial rate of monatin production observed with mutants Mutant 136, Mutant M, Mutant O, and Mutant BB was faster than the rate with the wild type control (SEQ ID NO:220). All the mutants showed improved monatin formation at 2 hours under the conditions tested. The lower than expected monatin titer at 1 hour for Mutant 135 was attributed to the inadvertent exposure to oxygen during the first hour. After 4 hours, the monatin titer was comparable between the mutants and the control under the conditions tested.

Example 35 Evaluation of the Impact of Temperature on Mutant DATs in BioReactors

This example describes data demonstrating the enzymatic activity of exemplary polypeptides disclosed herein under different temperature conditions. The wild type control (SEQ ID NO:220), Mutant 135 and Mutant M were produced in a fermentor at the 2.5 L scale as described in Example 15. At the end of fermentation, the cells were harvested by centrifugation at 5000-7000×g for 10 minutes and frozen as a wet cell paste at −80° C.

To prepare cell free extract containing the wild type control, Mutant 135 and Mutant M D-aminotransferases, 50 g of wet cell paste was suspended in 150 mL of 50 mM potassium phosphate buffer (pH 7.8) containing 0.05 mM pyridoxal phosphate (PLP) and then disrupted using a Microfluidics homogenizer (Microfluidics, Newton, Mass.) (3 passes at 18,000 psi), maintaining the temperature of the suspension at less than 15° C. Cellular debris was removed by centrifugation (20,000×g for 30 minutes).

The rate of formation of I3P from tryptophan was monitored at 340 nm for three minutes as described in Example 32. The concentration of the wild type control was determined to be 6.8 mg/mL, the concentration of Mutant 135 was determined to be 7.0 mg/mL and Mutant M was determined to be 5.6 mg/mL based on a standard curve generated with purified DAT wild type control. The DAT concentrations determined by I3P formation were used to dose the Infors to 0.2 mg/mL DAT. The aldolase was added as a cell free extract at 0.02 mg/mL aldolase. The reaction mix contained 10 mM potassium phosphate, 1 mM MgCl₂, 0.05 mM PLP, 200 mM sodium pyruvate and 130 mM D-tryptophan under a nitrogen headspace. Each of the DATs was evaluated for monatin production in a bioreactor at 35° C. and at 25° C.

Samples were taken at 0.5, 1, 3, 4 and 24 hours and analyzed using the LC/MS/MS methodology described in Example 36. The results are shown in Table 60.

TABLE 60 Fermenters at 25° and 35° C. Monatin Monatin Monatin Monatin Monatin DAT (mM) (mM) (mM) (mM) (mM) polypeptide 0.5 hr 1 hr 3 hr 4 hr 24 hr 25° C. Wild type 0.9 2.4 5.6 7.9 19.1 control (SEQ ID NO: 220) Mutant 135 1.6 4.4 10.9 12.1 18.6 Mutant M 2.1 4.5 9.4 12.4 17.4 35° C. Wild type 2.3 3.9 6.5 7.9 10.7 control (SEQ ID NO: 220) Mutant 135 4.1 6.1 9.8 11.5 14.9 Mutant M 4.1 6.3 9.9 11.3 14.9

As seen in Example 34, select mutant DATs yielded higher monatin titers at 35° C. compared to the wild type control DAT (SEQ ID NO:220). The wild type control DAT had a slower initial rate of monatin production but a higher final titer at 25° C. under the conditions tested. Both mutants Mutant 135 and Mutant M showed improved activity over the wild type control at 25° C. and 35° C. Mutants Mutant 135 and Mutant M had both a higher initial rate of monatin production and a higher final titer at 35° C. compared to the control under the conditions tested. The selected mutants were more stable than the wild type control at the higher temperatures. This indicates the advantages of GSSM and TMCA technologies in producing mutants with greater thermostability than the wild type control. One skilled in the arts could screen these GSSM or TMCA libraries for mutants with, for example, increased temperature tolerance.

Example 36 Detection of Monatin, MP, Tryptophan, Alanine, and HMG

This example describes the analytical methodology associated with the further characterization of exemplary D-aminotransferase (DAT) enzymes disclosed herein.

UPLC/UV Analysis of Monatin and Tryptophan

Analyses of mixtures for monatin and tryptophan derived from biochemical reactions were performed using a Waters Acquity UPLC instrument including a Waters Acquity Photo-Diode Array (PDA) absorbance monitor. UPLC separations were made using an Agilent XDB C8 1.8 μm 2.1×100 mm column (part #928700-906) (Milford, Mass.) at 23° C. The UPLC mobile phase consisted of A) water containing 0.1% formic acid B) acetonitrile containing 0.1% formic acid.

The gradient elution was linear from 5% B to 40% B, 0-4 minutes, linear from 40% B, to 90% B, 4-4.2 minutes, isocratic from 90% B to 90% B, 4.2-5.2 minutes, linear from 90% B to 5% B, 5.2-5.3 minutes, with a 1.2 minute re-equilibration period between runs. The flow rate was 0.5 mL/min, and PDA absorbance was monitored at 280 nm.

Sample concentrations are calculated from a linear least squares calibration of peak area at 280 nm to known concentration, with a minimum coefficient of determination of 99.9%.

Derivatization of Monatin Intermediates (Indole-3-Pyruvic Acid (I3P), Hydroxymethyloxyglutaric Acid, Monatin Precursor, and Pyruvate) with O-(4-Nitrobenzyl)hydroxylaminehydrochloride (NBHA)

In the process of monatin production, various intermediate compounds are formed and utilized. These compounds include: Indole-3-Pyruvic Acid (I3P), Hydroxymethyloxyglutaric Acid, Monatin Precursor, and Pyruvate. The ketone functional group on these compounds can be derivatized with O-(4-Nitrobenzyl)hydroxylamine hydrochloride (NBHA).

To 20 μL of sample or standard, 140 μL of NBHA (40 mg/mL in pyridine) was added in an amber vial. Samples were sonicated for 15 min in the presence of heat with occasional mixing. A 1:3 dilution in 35% Acetonitrile in water was performed.

UPLC/UV Analysis of Monatin Intermediates (Indole-3-Pyruvic Acid, Hydroxymethyloxyglutaric Acid, Monatin Precursor, and Pyruvate)

A Waters Acquity UPLC instrument including a Waters Acquity Photo-Diode Array (PDA) absorbance monitor (Waters, Milford, Mass.) was used for the analysis of the intermediate compounds. UPLC separations were made using a Waters Acquity HSS T3 1.8 mm×150 mm column (Waters, Milford, Mass.) at 50° C. The UPLC mobile phase consisted of A) water containing 0.3% formic acid and 10 mM ammonium formate and B) 50/50 acetonitrile/methanol containing 0.3% formic acid and 10 mM ammonium formate.

The gradient elution was linear from 5% B to 40% B, 0-1.5 minutes, linear from 40% B, to 50% B, 1.5-4.5 minutes, linear from 50% B to 90% B, 4.5-7.5 minutes, linear from 90% B to 95% B, 7.5-10.5 minutes, with a 3 minute re-equilibration period between runs. The flow rate was 0.15 mL/min from 0-7.5 minutes, 0.18 mL/min from 7.5-10.5 minutes, 0.19 mL/min from 10.5-11 minutes, and 0.15 mL/min from 11-13.5 minutes. PDA absorbance was monitored at 270 nm.

Sample concentrations were calculated from a linear least squares calibration of peak area at 270 nm to known concentration, with a minimum coefficient of determination of 99.9%.

Chiral LC/MS/MS (MRM) Measurement of Monatin

Determination of the stereoisomer distribution of monatin in biochemical reactions was accomplished by derivatization with 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide 30 (FDAA), followed by reversed-phase LC/MS/MS MRM measurement.

Derivatization of Monatin with FDAA

100 μL of a 1% solution of FDAA in acetone was added to 50 μL of sample or standard. Twenty μL of 1.0 M sodium bicarbonate was added, and the mixture was incubated for 1 hour at 40° C. with occasional mixing. The sample was removed and cooled, and neutralized with 20 μL of 2.0 M HCl (more HCl may be required to effect neutralization of a buffered biological mixture). Samples were analyzed by LC/MS/MS.

LC/MS/MS Multiple Reaction Monitoring for the Determination of the Stereoisomer Distribution of Monatin

Analyses were performed using the Waters/Micromass® liquid chromatography-tandem mass spectrometry (LC/MS/MS) instrument including a Waters 2795 liquid chromatograph with a Waters 996 Photo-Diode Array (PDA) absorbance monitor (Waters, Milford, Mass.) placed in series between the chromatograph and a Micromass® Quattro Ultima® triple quadrupole mass spectrometer. The LC separations capable of separating all four stereoisomers of monatin (specifically FDAA-monatin) were performed on a Phenomenex Luna® 2.0×250 mm (3 μm) C18 reversed phase chromatography column at 40° C. The LC mobile phase consisted of A) water containing 0.05% (mass/volume) ammonium acetate and B) acetonitrile. The elution was isocratic at 13% B, 0-2 minutes, linear from 13% B to 30% B, 2-15 minutes, linear from 30% B to 80% B, 15-16 minutes, isocratic at 80% B 16-21 minutes, and linear from 80% B to 13% B, 21-22 minutes, with a 8 minute re-equilibration period between runs. The flow rate was 0.23 mL/min, and PDA absorbance was monitored from 200 nm to 400 nm. All parameters of the ESI-MS were optimized and selected based on generation of deprotonated 20 molecular ions ([M−H]−) of FDAA-monatin, and production of characteristic fragment ions. The following instrumental parameters were used for LC/MS analysis of monatin in the negative ion ESI/MS mode: Capillary: 3.0 kV; Cone: 40 V; Hex 1: 15 V; Aperture: 0.1 V; Hex 2: 0.1 V; Source temperature: 120° C.; Desolvation temperature: 350° C.; Desolvation gas: 662 L/h; Cone gas: 42 L/h; Low mass resolution (Q1): 14.0; High mass resolution (Q1): 15.0; Ion energy: 0.5; Entrance: 0 V; Collision Energy: 20; Exit: 0 V; Low mass resolution (Q2): 15; High mass resolution (Q2): 14; Ion energy (Q2): 2.0; Multiplier: 650. Three FDAA-monatin-specific parent-to-daughter transitions were used to specifically detect FDAA-monatin in in vitro and in vivo reactions. The transitions monitored for monatin were 542.97 to 267.94, 542.97 to 499.07, and 542.97 to 525.04. Identification of FDAA-monatin stereoisomers was based on chromatographic retention time as compared to purified monatin stereoisomers, and mass spectral data.

Liquid Chromatography-Post Column Derivatization with OPA, Fluorescence Detection of Amino Acids, Including: Hydroxymethyl Glutamate (HMG) and Alanine

Analyses of mixtures for HMG and alanine derived from biochemical reactions were performed using a Waters Alliance 2695 and a Waters 600 configured instrument with a Waters 2487 Dual Wavelengths Absorbance Detector and Waters 2475 Fluorescence Detector as a detection system (Waters, Milford, Mass.). HPLC separations were made using two Phenomenex Aqua C18 125A, 150 mm×2.1 mm, 3μ, Cat #00F-4311B0 columns in series as the analytical columns, and a Phenomenex Aqua C18 125A, 30 mm×2.1 mm, 3μ, Cat #00A-4311B0 as an on-line solid phase extraction (SPE) column. Temperature for the two analytical columns was set at 55° C., and the on-line SPE column was at room temperature. The HPLC mobile phase consisted of A) 0.6% acetic acid with 1% methanol. The flow rate was (100% A) 0.2 mL/min from 0-3.5 minutes, 0.24 mL/min from 3.5-6.5 minutes, 0.26 mL/min from 6.5-10.4 minutes, and 0.2 mL/min from 10.4-11 minutes. UV-VIS absorbance detector was set to monitor at 336 nm wavelength. Fluorescence detector was set at 348 nm and 450 nm to monitor the excitation and emission wavelengths respectively.

Other Embodiments

It is to be understood that while the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other aspects, advantages, and modifications are within the scope of the following claims.

APPENDIX 1 Geneseq Geneseq Predic- SEQ NR Geneseq Protein Geneseq DNA ted Query Query Subject Subject % ID NR Accession NR NR Protein Accession DNA Accession EC DNA Protein DNA Protein % ID ID NO: Description Code Evalue Organism Description Code Evalue Description Code Evalue Number Length Length Length Length Protein DNA  1, 2 conserved 115385557 1.00E−125 Aspergillus Amino- ADS78245 1.00E−168 Amino- ADS78244 0 2.6.1.42 879 292 954 317 hypothetical terreus transferase/ transferase/ protein NIH2624 mutase/ mutase/ [Aspergillus deaminase deaminase terreus enzyme #14. enzyme #14. NIH2624]  3, 4 amino- 99078146 1.00E−96 Silicibacter Bacterial ADF03944 9.00E−69 Plant cDNA ADJ41018 2.4 2.6.1.21 855 284 0 286 66 transferase; sp. polypeptide #31 .class IV TM1040 #19. [Silicibacter sp TM1040]  5, 6 D-amino acid 39935662 8.00E−77 Rhodo- Bacterial ADF03944 1.00E−53 S. ADI39160 0.61 2.6.1.21 855 284 0 285 51 amino- pseudomonas polypeptide hygroscopicus transferase palustris #19. geldanamycin [Rhodo- CGA009 PKS AT1 pseudomonas mutant palustris fragment, CGA009] SEQ ID NO:83.  7, 8 D-alanine 119896473 1.00E−92 Azoarcus sp. P. stutzeri 4 AEM18040 7.00E−55 Klebsiella ABZ69309 0.16 2.6.1.21 870 289 0 285 58 transaminase BH72 D-HPG AT multi- [Azoarcus sp. outer forward copper BH72] N-term PCR oxidase. primer 1.  9, 10 amino- 114330773 2.00E−46 Nitrosomonas Prokaryotic ABU33175 2.00E−20 Prokaryotic ACA26397 0.28 2.6.1.21 435 144 0 286 60 transferase eutropha essential gene essential gene class IV C91 #34740. #34740. [Nitrosomonas eutropha C91] 11, 12 D-alanine 89360213 5.00E−85 Xanthobacter Bacterial aminotransferase autotrophicus polypeptide ADF03944 7.00E−55 Drosophila ABL17260 2.5 2.6.1.21 879 292 0 285 56 [Xanthobacter Py2 #19. melanogaster autotrophicus Dolypeptide Py2] SEQ ID gi|89350945|gb|E NO 24465 AS 16227.11 D-alanine aminotransferase [Xanthobacter autotrophicus Py2] 13, 14 D-amino acid 82745661 2.00E−99 Clostridium P. stulzeri 4 AEM18031 2.00E−46 Human gene AAH32576 0.039 2.6.1.21 855 284 0 282 63 aminotransferase beijerincki D-HPG 18-encoded [Clostridium NCIMB 8052 AT outer secreted beijerincki forward protein NCIMB 8052] N-term PCR HCUGC55, gi|82726488|gb|E primer 1 SEQ ID AP61226.1| D- NO: 185 amino acid aminotransferase [Clostridium Deijerincki NCIMB 8052] 15, 16 d-alanine 110834821 4.00E−56 Alcanivorax Bacillus AAY13560 2.00E−54 Bacterial ADE99771 0.038 2.6.1.21 837 278 0 294 45 aminotransferase borkumensis D-amino acid polypeptide [Alcanivorax SK2 amino- #19. borkumensis transferase. SK2] 17, 18 PUTATIVE 3122274 6.00E−49 Methano- Prokaryotic ABU21638 2.00E−45 0 2.6.1.42 918 305 0 306 34 BRANCHED- thermobacter essential gene CHAIN AMINO therm- #34740. ACID autotrophicus AMINOTRANSFE RASE (TRANSAMINAS E B) (BCAT). 19, 20 D-amino acid 82745661 7.00E−97 Clostridium P. stutzeri 4 AEM18031 1.00E−47 Drosophila ABL03988 2.4 2.6.1.21 861 286 0 282 61 aminotransferase beijerincki D-HPG melanogaster [Clostridium NCIMB AT outer polypeptide beijerincki NCIMB 8052 forward SEQ ID 8052] N-term NO 24465. gi|82726488|gb|E PCR AP61226.1| D- primer 1. amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 21, 22 D-amino acid 82745661 1.00E−100 Clostridium Mutant ABB08244 2.00E−47 Photorhabdus ACF71263 2.4 2.6.1.21 861 286 0 282 63 aminotransferase beijerincki Bacillus luminescens [Clostridium NCIMB sphaericus dat protein beijerincki NCIMB 8052 protein. sequence 8052] #59. gi|82726488|gb|E AP61226.1| D- amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 23, 24 D-amino acid 82745661 6.00E−96 Clostridium Mutant ABB08244 5.00E−46 Mouse ADA66322 0.16 2.6.1.21 861 286 0 282 61 aminotransferase beijerincki Bacillus mCG15938 [Clostridium NCIMB sphaericus gene beijerincki 8052 dat coding DNA NCIMB 8052] protein. (cDNA) gi|82726488|gb|E sequence. AP61226.1|D- amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 25, 26 D-amino acid 53802655 3.00E−86 Methylo- Staphylococcus ABM71198 2.00E−53 Human ABN23130 0.6 2.6.1.21 840 279 0 283 55 aminotransferase coccus aureus ORFX putative capsulatus protein #10. protein [Methylococcus str. Bath sequence capsulatus str. SEQ ID Bath] NO: 19716. 27, 28 aminotransferase, 119716594 9.00E−33 Nocardioides Escherichia AEK20408 3.00E−27 Human ADL15020 0.57 2.6.1.42 801 266 0 274 37 class IV sp. coli Toll/ [Nocardioides sp. JS614 amino- interleukin JS614]. transferase receptor-like gi|119537255|gb| ilvE protein for ABL81872.1| SEQ ID NO 2. cancer aminotransferase, treatment. class IV [Nocardioides sp. JS614] 29, 30 D-amino acid 15894079 3.00E−81 Clostridium Prokaryotic ABU32980 1.00E−45 Human AAK71577 0.6 2.6.1.21 849 282 843 280 51 51 aminotransferase aceto- essential gene immune/ [Clostridium butylicum #34740. haematopo acetobutylicum] ietic entigen genomic sequence SEQ ID NO: 41436. 31, 32 histidinol- 145644535 1.00E−40 Methano- Bacterial ADS43070 2.00E−37 Human ABA15896 0.003 2.6.19 1062 353 0 371 32 phosphate coccus polypeptide nervous aminotransferase maripaludis #10001. system related [Methanococcus c7 poly- maripaludis C7] nucleotide gi|145278069|gb| SEQ ID EDK17867.1| NO: 115893 histidinol- phosphate aminotransferase [Methanococcus maripaludis C7] 33, 34 D-ALANINE 118222 1.00E−108 Bacillus sp. P. stutzeri 4 AEM18018 1.00E−108 Listeria ABQ69245 4.00E−08 2.6.1.21 852 283 0 283 66 AMINOTRANSFE YM-1 D-HPGAT innocua RASE (D- outer DNA ASPARTATE forward sequence AMINOTRANSFE N-term #303. RASE) (D-AMINO PCR primer 1. ACID AMINOTRANSFE RASE) (D-AMINO ACID TRANSAMINASE) (DAAT). 35, 36 pyridoxal 126353148 2.00E−53 Caldivirga Klebsiella ABO62434 2.00E−31 Pseudomonas ABD13518 0.21 2.9.1.1 1143 380 0 388 35 phosphate- maquilin- pneumoniae aeruginosa dependent gensis polypeptide polypeptide enzyme, IC-167 seq id 7178. #3. putative [Caldivirga maquilingensis IC-167] gi|126311802|gb| EAZ64256.1| pyridoxal phosphate- dependent enzyme, putative [Caldivirga maquilingensis IC-167) 37, 38 glutamate-1- 149173540 1.00E−104 Planctomyces Bacterial ADN26446 2.00E−94 Human ABN18447 0.017 5.4.3.8 1389 462 0 455 43 semialdehyde 2;1- maris DSM polypeptide ORFX aminomutase; 8797 #10001. protein putative sequence [Planctomyces SEQ ID maris DSM 8797] NO: 19716. 39, 40 Serine-glyoxylate 148260372 1.00E−94 Acidiphilium Prokaryotic ABU21492 1.00E−68 Plant full ADO84697 0.05 2.6.1.45 1080 359 0 397 48 transaminase cryptum JF-5 essential gene length insert [Acidiphilium #34740. poly- cryptum JF-5] nucleotide seqid 4980. 41, 42 Chain A, 1127164 1.00E−126 Bacillus sp. P. stutzeri 4 AEM18037 1.00E−126 D-amino AAN81507 1.00E−20 2.6.1.21 852 283 0 282 76 Crystallographic YM-1 D-HPG acid Structure Of AT outer transaminase. D-Amino Acid forward Aminotransferase N-term PCR Complexed With primer 1. Pyridoxal-5 Phosphate. 43, 44 glutamate-1- 149173540 1.00E−115 Planctomyces Bacterial ADN26446 1.00E−95 Drosophila ABL08182 0.99 5.4.3.8 1350 449 0 455 48 semialdehyde 2;1- maris DSM polypeptide melanogaster aminomutase; 8797 #10001. Polypeptide putative SEQ ID [Planctomyces NO 24465. maris DSM 8797] 45, 46 Serine--glyoxylate 148260372 1.00E−111 Acidiphilium Prokaryotic ABU21492 1.00E−82 Prokaryotic ACA25362 6.00E−08 2.6.1.45 1170 389 0 397 51 transaminase cryptum JF-5 essential gene essential gene [Acidiphilium #34740. #34740. cryptum JF-5] 47, 48 glutamate-1- 149173540 1.00E−103 Planctomyces Bacterial ADN26446 1.00E−94 Human ACA62990 0.066 5.4.3.8 1401 466 0 455 42 semialdehyde 2:1- maris DSM polypeptide metal ion aminomuatse; 8797 #10001. transporter, putative 85080. [Planctomyces maris DSM 8797] 49, 50 aminotransferase 118061613 1.00E−108 Roseiflexus Bacterial ADN26446 4.00E−93 Bacterial ADS63519 7.00E−08 5.4.3.8 1353 450 0 454 49 class-III castenholzii Polypeptide Polypeptide [Roseiflexus DSM 13941 #10001. #10001. castenholzii DSM 13941] gi|118014341|gb| EAV28318.1| aminotransferase class-III [Roseiflexus castenholzii DSM 13941] 51, 52 aminotransferase 118061613 1.00E−107 Roseiflexus Bacterial ADN26446 8.00E−98 Clone ADH48029 1.00E−09 5.4.3.8 1344 447 0 454. 47 class-III castenholzii Polypeptide FS3-135 [Roseiflexus DSM 13941 #10001. DNA castenholzii DSM sequence 13941] SEQ ID gi|118014341|gb| NO: 2. EAV28318.1| aminotransferase class-III [Roseiflexus castenholzii DSM 13941] 53, 54 D-alanine 90962639 3.00E−70 Lactobacillus Staphylococcus ABM71198 2.00E−36 Prokaryotic ACA53304 2.4 2.6.1.21 861 286 0 281 49 aminotransferase salivarius aereus essential gene [Lactobacillus subsp. protein #10. #34740. salivarius subsp. salivarius salivarius UCC118 UCC118] 55, 56 aminotransferase; 148547264 2.00E−66 Pseudomonas Pseudomonas ABO84364 2.00E−55 Pseudomonas ABD17818 4.00E−34 2.6.1.1 399 132 0 396 94 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 57, 58 aminotransferase; 148547264 5.00E−66 Pseudomonas Pseudomonas ABO84364 6.00E−55 Pseudomonas ABD17818 6.00E−36 2.6.1.1 399 132 0 396 93 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 59, 60 aminotransferase; 148547264 1.00E−65 Pseudomonas Pseudomonas ABO84364 2.00E−54 Pseudomonas ABD17818 9.00E−29 2.6.1.1 399 132 0 396 93 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 61, 62 aminotransferase; 148547264 2.00E−66 Pseudomonas Pseudomonas ABO84364 2.00E−55 Pseudomonas ABD17818 1.00E−34 2.6.1.1 399 132 0 396 94 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 63, 64 aminotransferase; 148547264 1.00E−65 Pseudomonas Pseudomonas ABO84364 7.00E−56 Pseudomonas ABD17818 7.00E−48 2.6.1.1 399 132 0 396 93 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 65, 66 COG0436: 84317581 6.00E−59 Pseudomonas Pseudomonas ABO84364 2.00E−59 Pseudomonas ABD17818 5.00E−52 2.6.1.1 399 132 615 410 Aspartate/ aeruginosa aeruginosa aeruginosa tyrosine/ C3719 polypeptide #3. polypeptide aromatic #3. aminotransferase [Pseudomonas aeruginosa C3719] gi|126170242|gb| EAZ55753.1| aspartate transaminase [Pseudomonas aeruginosa C3719] 67, 68 COG0436: 84317581 1.00E−57 Pseudomonas Pseudomonas ABO84364 3.00E−58 Pseudomonas ABD17818 4.00E−46 2.6.1.1 399 132 615 410 Aspartate/ aeruginosa aeruginosa aeruginosa tyrosine/ C3719 polypeptide #3. polypeptide aromatic #3. aminotransferase [Pseudomonas aeruginosa C3719] gi|126170242|gb| EAZ55753.1| aspartate transaminase [Pseudomonas aeruginosa C3719] 69, 70 COG0436: 84317581 2.00E−58 Pseudomonas Pseudomonas ABO84364 5.00E−59 Pseudomonas ABD17818 1.00E−43 2.6.1.1 399 132 615 410 Aspartate/ aeruginosa aeruginosa aeruginosa tyrosine/ C3719 polypeptide #3. polypeptide aromatic #3. aminotransferase [Pseudomonas aeruginosa C3719] gi|126170242|gb| EAZ55753.1| 71, 72 aspartate 26990429 5.00E−68 Pseudomonas Pseudomonas ABO84364 1.00E−57 Pseudomonas ABD17818 5.00E−52 2.6.1.1 399 132 0 396 96 transaminase putida aeruginosa aeruginosa [Pseudomonas KT2440 polypeptide #3. polypeptide aeruginosa #3. C3719] aspartate aminotransferase [Pseudomonas putida KT2440] 73, 74 aspartate 26990429 3.00E−68 Pseudomonas Pseudomonas ABO84364 1.00E−57 Pseudomonas ABD17818 5.00E−52 2.6.1.1 399 132 0 396 96 aminotransferase putida aeruginosa aeruginosa [Pseudomonas KT2440 polypeptide polypeptide putida KT2440] #3. #3. 75, 76 D-alanine 94500389 1.00E−101 Oceanobacter L. AEB37927 2.00E−50 Pseudomonas ABD03911 2.5 2.6.1.21 873 290 0 265 57 transaminase sp. RED65 pneumophila aeruginosa [Oceanobacter sp. protein polypeptide RED65] SEQ ID #3. gi|94427424|gb|E NO 3367. AT12402.1| D- alanine transaminase [Oceanobacter sp. RED65] 77, 78 D-alanine 94500389 1.00E−101 Oceanobacter L. AEB37927 2.00E−50 Pseudomonas ABD03911 2.5 2.6.1.21 873 290 0 265 57 transaminase sp. RED65 pneumophila aeruginosa [Oceanobacter sp. protein SEQ polypeptide RED65] ID NO 3367. #3. gi|94427424|gb|E AT12402.1| D- alanine transaminase [Oceanobacter sp. RED65] 79, 80 aspartate 26990429 3.00E−64 Pseudomonas Pseudomonas ABO84364 8.00E−54 Pseudomonas ABD17818 7.00E−42 2.6.1.1 396 131 0 396 93 aminotransferase putida aeruginosa aeruginosa [Pseudomonas KT2440 polypeptide polypeptide putida KT2440] #3. #3. 81, 82 aspartate 26990429 4.00E−66 Pseudomonas Pseudomonas ABO84364 1.00E−55 Pseudomonas ABD17818 1.00E−40 2.6.1.1 396 131 0 396 94 aminotransferase putida aeruginosa aeruginosa [Pseudomonas KT2440 polypeptide polypeptide putida KT2440] #3. #3. 83, 84 glutamate-1- 149173540 1.00E−101 Planctomyces Bacterial ADN26446 2.00E−93 Bacterial ADS58845 0.017 5.4.3.8 1398 465 0 455 41 semialdehyde 2;1- maris DSM polypeptide polypeptide aminomutase; 8797 #10001. #10001. putative [Planctomyces maris DSM 8797] 85, 86 aminotransferase; 114319339 1.00E−69 Alkalilim- L. pneumophila AEB37927 1.00E−46 Myco- AAI99682 0.62 2.6.1.21 873 290 0 286 46 class IV nicola protein SEQ bacterium [Alkalilimnicola ehrlichei ID NO 3367. tuberculosis ehrlichei MLHE-1 strain H37Rv MLHE-1] genome SEQ ID NO 2. 87, 88 D-alanine 74316285 3.00E−55 Thiobacillus P. stutzeri 4 AEM18040 2.00E−49 DNA done ACL13803 9.8 2.6.1.21 879 292 0 282 40 transminase denitrificans D-HPG originating [Thiobacillus ATCC 25259 AT outer in barley denitrificans forward N-term containing ATCC 25259] SNP PCR primer 1. sequence #14. 89, 90 aminotransferase; 148547264 5.00E−66 Pseudomonas Mycobacterium ABO84364 1.00E−54 Pseudomonas ABD17818 4.00E−34 2.6.1.1 399 132 0 396 93 class I and II putida F1 tuberculosis aeruginosa [Pseudomonas strain polypeptide putida F1] Mycobacterium #3. 91, 92 aminotransferase; 148547264 4.00E−65 Pseudomonas Pseudomonas ABO84364 4.00E−56 Pseudomonas ABD17818 3.00E−53 2.6.1.1 399 132 0 396 93 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 93, 94 aminotransferase; 148547264 4.00E−65 Pseudomonas Pseudomonas ABO84364 4.00E−56 Pseudomonas ABD17818 8.00E−54 2.6.1.1 399 132 0 396 93 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 95, 96 aminotransferase; 148547264 4.00E−65 Pseudomonas Pseudomonas ABO84364 4.00E−56 Pseudomonas ABD17818 8.00E−54 2.6.1.1 399 132 0 396 93 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 97, 98 aminotransferase; 148547264 1.00E−65 Pseudomonas Pseudomonas ABO84364 1.00E−56 Pseudomonas ABD17818 5.00E−55 2.6.1.1 399 132 0 396 93 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 99, aspartate 26990429 1.00E−65 Pseudomonas Pseudomonas ABO84364 3.00E−56 Pseudomonas ABD17818 3.00E−44 2.6.1.1 393 130 0 396 96 100 aminotransferase putida aeruginosa aeruginosa [Pseudomonas KT2440 polypeptide #3. polypeptide putida KT2440] #3. 101, aspartate 26990429 7.00E−68 Pseudomonas Pseudomonas ABO84364 3.00E−57 Pseudomonas ABD17818 8.00E−54 2.6.1.1 396 131 0 396 97 102 aminotransferase putida aeruginosa aeruginosa [Pseudomonas KT2440 polypeptide #3. polypeptide putida KT2440] #3. 103, aspartate 26990429 5.00E−66 Pseudomonas Pseudomonas ABO84364 3.00E−57 Pseudomonas ABD17818 8.00E−60 2.6.1.1 399 132 0 396 95 104 aminotransferase putida aeruginosa aeruginosa [Pseudomonas KT2440 polypeptide #3. polypeptide putida KT2440] #3. 105, aspartate 26990429 2.00E−66 Pseudomonas Pseudomonas ABO84364 1.00E−57 Pseudomonas ABD17818 5.00E−55 2.6.1.1 399 132 0 396 94 106 aminotransferase putida aeruginosa aeruginosa [Pseudomonas KT2440 polypeptide #3. polypeptide putida KT2440] #3. 107, aminotransferase; 148547264 7.00E−65 Pseudomonas Pseudomonas ABO84364 3.00E−55 Pseudomonas ABD17818 8.00E−54 2.6.1.1 399 132 0 396 93 108 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 109, aminotransferase; 148547264 1.00E−65 Pseudomonas Pseudomonas ABO84364 7.00E−56 Pseudomonas ABD17818 5.00E−49 2.6.1.1 399 132 0 396 93 110 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 111, aminotransferase; 148547264 3.00E−66 Pseudomonas Pseudomonas ABO84364 1.00E−56 Pseudomonas ABD17818 5.00E−52 2.6.1.1 393 132 0 396 94 112 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 113, aminotransferase; 148547264 1.00E−65 Pseudomonas Pseudomonas ABO84364 1.00E−56 Pseudomonas ABD17818 3.00E−50 2.6.1.1 396 132 0 396 93 114 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 115, aspartate 26990429 1.00E−65 Pseudomonas Pseudomonas ABO84364 2.00E−56 Pseudomonas ABD17818 5.00E−52 2.6.1.1 399 130 0 396 96 116 aminotransferase putida aeruginosa aeruginosa [Pseudomonas KT2440 polypeptide #3. polypeptide putida KT2440] #3. 117, aminotransferase; 148547264 4.00E−65 Pseudomonas Pseudomonas ABO84364 4.00E−56 Pseudomonas ABD17818 5.00E−55 2.6.1.1 399 132 0 396 93 118 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 119, aminotransferase; 148547264 1.00E−65 Pseudomonas Pseudomonas ABO84364 1.00E−56 Pseudomonas ABD17818 5.00E−55 2.6.1.1 399 132 0 396 93 120 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. 121, aminotransferase; 148547264 4.00E−65 Pseudomonas Pseudomonas ABO84364 4.00E−56 Pseudomonas ABD17818 3.00E−56 2.6.1.1 399 132 0 396 93 122 class I and II putida F1 aeruginosa aeruginosa [Pseudomonas polypeptide #3. polypeptide putida F1] #3. aspartate 123, aminotransferase 26990429 8.00E−66 Pseudomonas Pseudomonas ABO84364 2.00E−56 Pseudomonas ABD17818 3.00E−38 2.6.1.1 399 132 0 396 93 124 [Pseudomonas putida aeruginosa aeruginosa putida KT2440] KT2440 polypeptide #3. polypeptide D-alanine #3. 125, aminotransferase 29833346 4.00E−77 Streptomyces Escherichia AEK20408 2.00E−33 Bacterial ADS45796 0.038 2.6.1.42 831 132 0 273 57 126 [Streptomyces avermitilis coli polypeptide avermitilis MA-4680 amino- #10001. MA-4680] transferase ilvE SEQ ID NO 2. 127, glutamate-1- 149173540 1.00E−143 Planctomyces Bacterial ADN26446 1.00E−100 Clone FS-135 ADH48029 1.00E−09 5.4.3.8 1362 132 0 455 55 128 semialdehyde 2;1- maris DSM polypeptide DNA aminomutase; 8797 #10001. sequence putative SEQ ID [Planctomyces NO: 2. maris DSM 8797] 129, D-alanine 77465457 1.00E−87 Rhodobacter Bacterial ADF03944 3.00E−68 Drosophila ABL03211 2.4 2.6.1.21 864 132 0 285 57 130 aminotransferase sphaeroides polypeptide melanogaster [Rhodobacter 2.4.1 #19. polypeptide sphaeroides SEQ ID 2.4.1] NO 24465. 131, D-Amino Acid 126651304 1.00E−158 Bacillus sp. P. taetrolens ADW43694 1.00E−159 B. sphaericus ADP27941 0 2.6.1.21 855 284 1709 284 132 Aminotransferase B14905 aldolase 2. D-amino- [Bacillus sp. transferase B14905] BSDAT SEQ gi|126591833|gb| ID NO: 4. EAZ85916.1| D- Amino Acid Aminotransferase [Bacillus sp. B14905] 133, aminotransferase 56477154 4.00E−67 Azoarcus sp. Prokayotic ABU33175 4.00E−45 M. xanthus ACL64145 0.16 2.6.1.21 876 291 0 285 45 134 class-IV EbN1 essential gene protein [Azoarcus sp. #34740. sequence, EbN1] seq id 9726. 135, D-alanine 77465457 1.00E−87 Rhodobacter Bacterial ADF03944 3.00E−68 Drosophila ABL03211 2.4 2.6.1.21 864 287 0 285 57 136 aminotransferase sphaeroides polypeptide melanogaster [Rhodobacter 2.4.1 #19. polypeptide sphaeroides SEQ ID 2.4.1] NO 24465. 137, glutamate-1- 149173540 1.00E−111 Planctomyces Bacterial ADN26446 1.00E−104 P. cellulosum AEC75821 0.001 5.4.3.8 1386 461 0 455 46 138 semialdehyde 2;1- maris polypeptide ambruticin aminomutase; DSM 8797 #10001. ambA putative protein. [Planctomyces maris DSM 8797] 139, glutamate-1- 149173540 1.00E−108 Planctomyces Bacterial ADN26446 7.00E−99 Bacterial ADS57580 0.065 5.4.3.8 1383 460 0 455 43 140 semialdehyde 2;1- maris DSM polypeptide polypeptide aminomutase; 8797 #10001. #10001. putative [Planctomyces maris DSM 8797] 141, Putative D-alanine 146342961 2.00E−71 Bradyr- Bacterial ADF03944 1.00E−61 N. ABZ41029 0.15 2.6.1.21 855 284 0 286 51 142 aminotransferase hizobium polypeptide gonorrhoeae [Bradyrhizobium sp. ORS278 #19. nucleotide sp. ORS278] sequence SEQ ID 4691. 143, Aminotransferase, 88806011 1.00E−101 Robiginitalea Bacillus AAY13560 2.00E−48 DNA AAS82939 0.66 2.6.1.21 915 304 0 255 57 144 class IV biformata D-amino acid encoding [Robiginitalea HTCC2501 amino- novel human biformata transferase diagnostic HTCC2501] protein gi|88783620|gb|E #20574. AR14791.1| Aminotransferase, class IV [Robiginitalea biformata HTCC2501] 145, glutamate-1- 149173540 1.00E−114 Planctomyces Bacterial ADN26446 1.00E−93 M. xanthus ACL64704 0.99 5.4.3.8 1350 449 0 455 48 146 semialdehyde 2;1- maris DSM polypeptide protein aminomutase; 8797 #10001. sequence, putative seq id [Planctomyces 9726. maris DSM 8797] 147, aminotransferase 118061613 1.00E−104 Roseiflexus Bacterial ADN26446 2.00E−87 Glutamate-1- AAQ63611 2.00E−05 5.4.3.8 1383 460 0 454 44 148 class-III castenholzii polypeptide semialdehyde [Roseiflexus DSM 13941 #10001. amino- castenholzii DSM transferase 13941] gi|118014341 |gb| EAV28318.1| aminotransferase class-III [Roseiflexus castenholzii DSM 13941] 149, glutamate-1- 149173540 1.00E−114 Planctomyces Bacterial ADN26446 1.00E−90 Bacterial ADS57112 0.004 5.4.3.8 1377 458 0 455 46 150 semialdehyde 2;1- maris polypeptide polypeptide aminomutase; DSM 8797 #10001. #10001. putative [Planctomyces maris DSM 8797] 151, D-alanine 52079452 1.00E−56 Bacillus P. stutzeri 4 AEM18039 2.00E−56 Human ADE53957 2.4 2.6.1.21 852 283 0 283 40 152 aminotransferase licheniformis D-HPG prostate [Bacillus ATCC 14580 AT outer cancer cDNA licheniformis forward #473. ATCC 14580] N-term PCR primer 1. 153, glutamate-1- 149173540 1.00E−97 Planctomyces Bacterial ADN26446 2.00E−74 M. xanthus ACL64518 0.24 5.4.3.8 1269 422 0 455 46 154 semialdehyde 2;1- maris DSM polypeptide protein aminomutase; 8797 #10001. sequence, putative seq id [Planctomyces 9726 maris DSM 8797] 155, aminotransferase 118045454 1.00E−107 Chloroflexus Bacterial ADN26446 1.00E−104 Bacterial ADT43944 3.00E−04 5.4.3.8 1362 453 0 443 46 156 class-III aggregans polypeptide polypeptide [Chloroflexus DSM 9485 #10001. #10001. aggregans DSM 9485] gi|117997930|gb| EAV12112.1| aminotransferase class-III [Chloroflexus aggregans DSM 9485] 157, aminotransferase, 12675091 2.00E−78 Rhodo- Bacterial ADF03944 9.00E−53 Oligonudeo- ABQ42663 2.5 2.6.1.21 879 292 0 283 48 158 class IV bacterales polypeptide tide for [Rhodobacterales bacterium #19. detecting bacterium HTCC2150 cytosine HTCC2150] methylation gi|126706255|gb| SEQ ID NO EBA05345.1| 20311. aminotransferase, class IV [Rhodobacterales bacterium HTCC2150] 159, hypothetical 126646718 6.00E−43 Algoriphagus prokaryotic ABU18963 7.00E−26 prokaryotic ACA38856 0.58 2.6.1.42 822 273 0 277 36 160 protein sp. essential essential ALPR1J8298 PR1 gene gene [Algoriphagus #34740. #34740. sp. PR1] gi|126576766|gb| EAZ81014.1| hypothetical protein ALPR1J8298 [Algoriphagus sp. PR1] 161, D-amino acid 86140221 1.00E−139 Roseobacter Bacterial ADF03944 5.00E−75 Bacterial ADS56887 2.4 2.6.1.21 861 286 0 287 87 162 aminotransferase, sp. polypeptide polypeptide putative MED193 #19. #19. [Roseobacter sp. MED193] gi|85823158|gb|E AQ43371.1| D- amino acid aminotransferase, putative [Roseobacter sp. MED193] 163, glutamate-1- 149173540 1.00E−105 Planctomyces Bacterial ADN26446 6.00E−96 M. xanthus ACL64750 0065 5.4.3.8 1377 458 0 455 44 164 semialdehyde maris DSM polypeptide protein 2;1- 8797 #10001. sequence, aminomutase; seq id 9726. putative [Planctomyces maris DSM 8797] 165, aminotransferase 148656729 1.00E−105 Roseiflexus Bacterial ADN26446 1.00E−95 Bacterial ADS63519 7.00E−11 5.4.3.8 1314 437 0 455 46 166 class-III sp. RS-1 polypeptide polypeptide [Roseiflexus sp. #10001. #10001. RS-1] 167, D-amino acid 82745661 8.00E−96 Clostridium P. stutzeri 4 AEM18031 2.00E−47 Drosophila ABL03988 2.4 2.6.1.21 861 286 0 282 60 168 aminotransferase beijerincki D-HPG melanogaster [Clostridium NCIMB AT outer polypeptide beijerincki 8052 forward SEQ ID NO NCIMB 8052] N-term 24465. gi|82726488|gb|E PCR AP61226.1| D- primer 1. amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 169, putative amino 120406056 1.00E−144 Myco- Prokaryolic ABU33708 1.00E−124 Prokaryolic ACA37578 2.00E−53 4.1.3.38 882 293 0 293 65 170 acid bacterium essential essential aminotransferase vanbaalenii gene gene [Mycobacterium PYR-1 #34740. #34740. vanbaalenii PYR- gi|119958874|gb| ABM 15879.11 putative amino acid aminotransferase [Mycobacterium vanbaalenii PYR-1] 171, D-amino acid 82745661 3.00E−99 Clostridium P. stutzeri 4 AEM18031 1.00E−47 Drosophila ABL03988 2.4 2.6.1.21 864 267 0 262 62 172 aminotransferase beijerincki D-HPG melanogaster [Clostridium NCIMB AT outer polypeptide beijerincki 8052 forward SEQ ID NCIMB N-term NO 24465. 8052] PCR gi|82726488|gb|E primer 1. AP61226.1] D-amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 173, D-amino acid 82745661 1.00E−98 Clostridium Mutant ABB08244 2.00E−47 Fibrotic AED18099 0.039 2.6.1.21 861 286 0 282 62 174 aminotransferase beijerincki Bacillus disorder [Clostridium NCIMB sphaericus associated beijerincki 8052 dat protein. poly- NCIMB nudeotide 8052] SEQ ID gi|82726488|gb|E NO 9. AP61226.1| D-amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 175, D-amino acid 82745661 7.00E−98 Clostridium Mutant ABB08244 1.00E−46 Human AAF81809 0.61 2.6.1.21 861 266 0 282 62 176 aminotransferase beijerincki Bacillus secreted [Clostridium NCIMB sphaericus protein beijerincki 8052 dat protein. sequence NCIMB 8052] encoded by gi|82726488|gb|E gene 11 AP61226.1) D- SEQ ID amino acid NO: 76. aminotransferase [Clostridium beijerincki NCIMB 8052] 177, aminotransferase 118061613 1.00E−110 Roseiflexus Bacterial ADN26446 9.00E−89 Hyper- ADM27081 0.004 5.4.3.8 1374 457 0 454 47 178 class-III castenholzii polypeptide thermophile [Roseiflexus DSM 13941 #10001. Methanopyrus castenholzii kandleri DSM 13941] protein #26. gi|118014341 |gb| EAV28318.1| aminotransferase class-III [Roseiflexus castenholzii DSM 13941] 179, D-amino acid 124520974 4.00E−54 Bacillus Staphyloco- ABM71198 5.00E−54 Plant full ADX27642 2.4 2.6.1.21 855 264 0 288 41 180 aminotransferase coagulans ccus length [Bacillus 36D1 aureus insert coagulans 36D1] protein #10. polynudeotide gi|124497181|gb| seqid 4980. EAY44748.1| D-amino acid aminotransferase [Bacillus coagulans 36D1] 181, branched-chain 22299586 7.00E−53 Thermo- Prokaryolic ABU18055 1.00E−47 Prostate ABL61996 0.62 2.6.1.42 876 291 861 286 39 51 182 amino acid synecho- essential cancer aminotransferase coccus gene related gene [Thermo- elongatus #34740. sequence synechococcus BP-1 SEQ ID elongatus NO: 8120. BP-1]. 183, D-alanine 23098538 2.00E−53 Oceano- P. stutzeri 4 AEM18040 1.00E−51 Plasmid ADY80523 2.3 2.6.1.21 825 274 867 288 41 51 184 aminotransferase bacillus D-HPG pMRKAd5 [Oceanobacillus iheyensis AT outer HIV-1 gag iheyensis]. forward DNA N-term noncoding PCR sequence primer 1. SEQ ID NO: 26. 185, D-alanine 23098538 2.00E−53 Oceano- P. stutzeri 4 AEM18040 1.00E−51 Plasmid ADY80523 2.3 2.6.1.21 825 274 867 288 41 51 186 aminotransferase bacillus D-HPG pMRKAd5 [Oceanobacillus iheyensis AT outer HIV-1 gag iheyensis]. forward DNA N-term noncoding PCR sequence primer 1. SEQ ID NO: 26. 187, D-amino acid 82745661 1.00E−96 Clostridium P. stutzeri 4 AEM18031 3.00E−46 Human AAL04589 2.4 2.6.1.21 861 286 0 282 61 188 aminotransferase beijerincki D-HPG repdroductive [Clostridium NCIMB AT outer system beijerincki 8052 forward related NCIMB 8052] N-term antigen gi|82726488|gb|E PCR DNA SEQ AP61226.1] D- primer 1. ID NO: 8114. amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 189, D-amino acid 82745661 9.00E−98 Clostridium Mutant ABB08244 2.00E−49 Drosophila ABL25490 0.62 2.6.1.21 864 287 0 282 62 190 aminotransferase beijerincki Bacillus melanogaster [Clostridium NCIMB sphaericus polypeptide beijerincki 8052 dat protein. SEQ ID NCIMB 8052] NO 24465. gi|82726488|gb|E AP61226.1] D- amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 191, D-amino acid 82745661 1.00E−96 Clostridium P. stutzeri 4 AEM18031 3.00E−46 Human AAL04589 2.4 2.6.1.21 861 286 0 262 61 192 aminotransferase beijerincki D-HPG repdroductive [Clostridium NCIMB AT outer system beijerincki 8052 forward related NCIMB 8052] N-term antigen gi|82726488|gb|E PCR DNA SEQ AP61226.1] D- primer 1. ID NO: 8114. amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 193, D-amino acid 82745661 1.00E−96 Clostridium P. stutzeri 4 AEM18031 3.00E−46 Human AAL04589 2.4 2.6.1.21 861 286 0 282 61 194 aminotransferase beijerincki D-HPG repdroductive [Clostridium NCIMB AT outer system beijerincki 8052 forward related antigen NCIMB 8052] N-term DNA SEQ gi|82726488|gb|E PCR ID NO: 8114. AP61226.1] D- primer 1. amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 195, putative 149182609 1.00E−59 Bacillus sp. Bacillus AAY13560 1.00E−57 Human ADL41705 0.61 2.6.1.21 858 265 0 282 42 196 D-alanine SG-1 D-amino acid ovarian aminotransferase amino- cancer DNA [Bacillus sp. transferase. marker #5. SG-1] 197, putative 149182609 3.00E−57 Bacillus sp. P. stutzeri 4 AEM18039 6.00E−56 P. stutzeri 4 AEM18017 0.003 2.6.1.21 858 285 0 282 40 198 D-alanine SG-1 D-HPG D-HPG aminotransferase AT outer AT outer [Bacillus sp. forward forward SG-1] N-term N-term putative PCR PCR primer 1. primer 1. 199, D-alanine 149182609 5.00E−59 Bacillus sp. Bacillus AAY13560 1.00E−57 Bacillus ABK78375 2.4 2.6.1.21 858 285 0 282 42 200 aminotransferase SG-1 D-amino acid licheniformis [Bacillus sp. amino- genomic SG-1] transferase. sequence tag (GST) #933. 201, D-alanine 51892468 6.00E−57 Symbio- Bacillus AAY13560 2.00E−51 Photorhabdus ACF67498 0.15 2.6.1.21 855 284 0 281 40 202 aminotransferase bacterium D-amino acid luminescens [Symbiobacterium thermophilum amino- protein thermophilum IAM 14863 transferase. sequence #59. IAM 14863] 203, PUTATIVE 3122274 2.00E−46 Methano Prokaryotic ABU23351 6.00E−42 Prokaryotic ACA40289 0.17 2.6.1.42 921 306 0 306 34 204 BRANCHED- thermobacter essential essential gene CHAIN AMINO thermauto- gene #34740. ACID trophicus #34740. AMINO- TRANSFERASE (TRANSAMINAS E B) (BCAT). 205, D-alanine 23098538 2.00E−53 Oceano- P. stutzeri 4 AEM18040 1.00E−51 Plasmid ADY80523 2.3 2.6.1.21 825 274 867 288 41 51 206 aminotransferase bacillus D-HPG pMRKAd5 [Oceanobacillus iheyensis AT outer HIV-1 gag iheyensis]. forward DNA N-term noncoding PCR sequence primer 1. SEQ ID NO: 26. 207, D-alanine 23098538 7.00E−51 Oceano- P. stutzeri 4 AEM18040 4.00E−50 Human ABO99281 0.15 2.6.1.21 825 274 867 288 40 48 208 aminotransferase bacillus D-HPG protein [Oceanobacillus iheyensis AT outer SEQ ID 537. iheyensis]. forward N-term PCR primer 1. 209, aminotransferase 118061613 1.00E−105 Roseiflexus Bacterial ADN26446 3.00E−88 Clone ADH48029 0.001 5.4.3.8 1374 457 0 454 44 210 class-III castenholzii polypeptide FS3-135 [Roseiflexus DSM 13941 #10001. DNA castenholzii sequence DSM 13941] SEQ ID gi|118014341 |gb| NO: 2 EAV28318.1| aminotransferase class-III [Roseiflexus castenholzii DSM 13941] 211, hypothetical 145952948 6.00E−42 Clostridium Bacterial ADS43070 4.00E−25 Human AAI92131 0.053 2.6.1.9 1137 378 0 367 29 212 protein difficile polypeptide polynucleoide CdifQ_04002916 QCD- #10001. SEQ ID NO [Clostridium 32g58 13646. difficile QCD- 32g58] 213, D-amino acid 82745661 3.00E−95 Clostridium P. stutzeri 4 AEM18018 2.00E−52 Plant full ADX37088 0.6 2.6.1.21 849 262 0 282 61 214 aminotransferase beijerincki D-HPG length [Clostridium NCIMB AT outer insert beijerincki 8052 forward poly- NCIMB 8052] N-term nudeotide gi|82726488|gb|E PCR seqid 4980. AP61226.1] D- primer 1. amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 215, aminotransferase 118061613 1.00E−116 Roseiflexus Bacterial ADN26446 1.00E−102 Bacterial ADS57580 0.004 5.4.3.8 1386 461 0 454 49 216 class-III castenholzii polypeptide polypeptide [Roseiflexus DSM 13941 #10001. #10001. castenholzii DSM 13941] gi|118014341 |gb| EAV28318.1] aminotransferase class-III [Roseiflexus castenholzii DSM 13941] 217, D-alanine 23098538 2.00E−53 Oceano- P. stutzeri 4 AEM18040 1.00E−51 Plasmid ADY80523 2.3 2.6.1.21 825 274 867 288 41 51 218 aminotransferase bacillus D-HPG pMRKAd5 [Oceanobacillus iheyensis AT outer HIV-1 gag iheyensis]. forward DNA N-term noncoding PCR sequence SEQ primer 1. ID NO: 26. 219, D-amino acid 82745661 3.00E−98 Clostridium Mutant ABB08244 4.00E−48 Human ABZ36033 0.16 2.6.1.21 861 286 0 282 62 220 aminotransferase beijerincki Bacillus secretory [Clostridium NCIMB sphaericus poly- beijerincki 8052 dat protein. nucleotide NCIMB 8052] SPTM gi|82726488|gb|E SEQ ID NO AP61226.1] D- 534. amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 221, D-alanine 51892468 1.00E−57 Symbio- Bacillus AAY13560 3.00E−54 Human ACN44196 2.3 2.6.1.21 840 279 0 281 43 222 aminotransferase bacterium D-amino acid protein [Symbiobacterium thermophilum amino- sequence thermophilum IAM 14863 transferase. hCP39072. IAM 14863] 223, D-alanine 23098538 3.00E−97 Oceano- Heat ABB06297 7.00E−90 L. salivarius AFB66287 0.62 2.6.1.21 867 288 867 288 60 63 224 aminotransferase bacillus resistant D- SIA [Oceanobacillus iheyensis amino acid protein gene iheyensis]. amino- LSL1401b transferase (partial). encoding DNA. 225, D-amino acid 82745661 7.00E−97 Clostridium Mutant ABB08244 6.00E−47 Fibrotic AED18099 0.039 2.6.1.21 861 266 0 282 61 226 aminotransferase beijerincki Bacillus disorder [Clostridium NCIMB sphaericus associated beijerincki 8052 dat protein. poly- NCIMB 8052] nucleotide gi|82726488|gb|E SEQ ID AP61226.1] D- NO 9. amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 227, D-amino acid 82745661 4.00E−98 Clostridium P. stutzeri 4 AEM18031 9.00E−47 Fibrotic AED18099 0.61 2.6.1.21 861 286 0 282 62 228 aminotransferase beijerincki D-HPG disorder [Clostridium NCIMB 8052 AT outer associated beijerincki forward poly- NCIMB 8052] N-term nucleotide gi|82726488|gb|E PCR SEQ ID AP61226.1| D- primer 1. NO 9. amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 229, D-amino acid 82745661 3.00E−94 Clostridium Mutant ABB08244 9.00E−45 Fibrotic AED18099 0.62 2.6.1.21 873 290 0 282 60 230 aminotransferase beijerincki Bacillus disorder [Clostridium NCIMB 8052 sphaericus associated beijerincki dat protein. poly- NCIMB 8052] nucleotide gi|82726400|gb|E SEQ ID AP61226.1| D- NO 9. amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 231, D-amino acid 82745661 2.00E−97 Clostridium P. stutzeri 4 AEM18031 5.00E−48 Fibrotic AED18099 0.62 2.6.1.21 864 287 0 282 61 232 aminotransferase beijerincki D-HPG disorder [Clostridium NCIMB AT outer associated beijerincki 8052 forward poly- NCIMB 8052] N-term nucleotide gi|82726488|gb|E PCR SEQ ID AP61226.1] D- primer 1. NO 9. amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 233, hypothetical 126646718 2.00E−43 Algoriphagus B. AEJ13860 1.00E−26 Drosophila ABL22310 0.58 2.6.142 822 273 0 277 35 234 protein sp. PR1 licheniformis melanogaster ALPR1_18298 butyryl-CoA polypeptide [Algoriphagus dehy- SEQ ID sp. PR1] drogenase/acy NO 24465. gi|126576766|gb| I-CoA EAZ81014.1| dehy- hypo- drogenase theticalprotein pro. 1. ALPR1_18298 [Algoriphagus sp. PR1] 235, branched-chain 18313971 1.00E−43 Pyrobaculum Aquifex ABU57358 4.00E−44 Bacterial ADT43796 0.65 2.6.1.42 906 301 1992 303 236 amino acid aerophilum aspartate polypeptide aminotransferase amino- #10001. (ilvE) transferase B [Pyrobaculum DNA. aerophilum]. 237, d-alanine 110834821 1.00E−55 Alcanivorax Bacillus AAY13560 4.00E−55 Wheat AAI70509 2.3 2.6.1.21 837 278 0 294 44 238 aminotransferase borkumensis D-amino tryptophan [Alcanivorax SK2 acid amino- decarboxylase. borkumensis transferase. SK2] 239, d-alanine 110834821 2.00E−54 Alcanivorax Bacillus AAY13560 4.00E−44 P. stutzeri 4 AEM18017 0.038 2.6.1.21 843 280 870 282 240 aminotransferase borkumensis D-amino D-HPG [Alcanivorax SK2 acid amino- AT outer borkumensis transferase. forward SK2] N-term PCR primer 1. 241, D-alanine 23098538 2.00E−53 Oceano- P. stutzeri 4 AEM18040 1.00E−51 Plasmid ADY80523 2.3 2.6.1.21 825 274 867 288 41 51 242 aminotransferase bacillus D-HPG pMRKAd5 [Oceanobacillus iheyensis AT outer HIV-1 gag iheyensis]. forward DNA N-term noncoding PCR sequence primer 1. SEQ ID NO: 26. 243, D-alanine 23098538 2.00E−53 Oceano- P. stutzeri 4 AEM18040 1.00E−51 Plasmid ADY80523 2.3 2.6.1.21 825 274 867 288 41 51 244 aminotransferase bacillus D-HPG pMRKAd5 [Oceanobacillus iheyensis AT outer HIV-1 gag iheyensis]. forward DNA N-term noncoding PCR sequence primer 1. SEQ ID NO: 26. 245, aromatic amino 104781758 1.00E−153 Pseudomonas Klebsiella ABO61955 1.00E−130 Bacterial ADS56672 2.00E−28 2.6.1.57 909 302 0 398 90 246 acid entomophila pneumoniae polypeptide aminotransferase L48 polypeptide #10001. [Pseudomonas seqid 7178. entomophila L48] 247, D-amino acid 82745661 6.00E−99 Clostridium Mutant ABB08244 5.00E−46 Mouse ABA93421 0.61 2.6.1.21 861 286 0 282 62 248 aminotransferase beijerincki Bacillus arylacetamide [Clostridium NCIMB sphaericus deacetylase beijerincki 8052 dat protein. related NCIMB 8052] protein gi|82726488|gb|E SEQ AP61226.1| D- ID NO: 5. amino acid aminotransferase [Clostridium beijerincki NCIMB 8052] 249, putative 72162511 8.00E−29 Thermobifida Escherichia AEK20408 4.00E−23 M. xanthus ACL64761 0.15 2.6.1.42 837 278 0 280 34 250 aminotransferase fusca YX coli protein [Thermobifida amino- sequence, seq fusca YX] transferase id 9726. ilvE SEQ ID NO 2 251, D-amino-acid 91775144 6.00E−17 Methylo- Enterobacter AEH60497 5.00E−14 P. ADQ03059 8.00E−04 1.4.99.1 138 45 0 417 93 252 dehydrogenase bacillus cloacae aeruginosa [Methylobacillus flagellatus protein virulence flagellatus KT] KT amino acid gene, sequence - VIR14, SEQ ID 5666. protein. 253, D-amino acid 32473614 1.00E−152 Rhodo- N. ABP80542 6.00E−46 Arabidopsis ADA71348 0.92 1.4.99.1 1260 419 0 456 61 254 dehydrogenase; pirellula gonorrhoeae thaliana small chain baltica SH 1 nucleotide protein, [Rhodopirellula sequence SEQ SEQ ID 1971. baltica SH 1] ID 4691. 255, D-amino-acid 91779297 0 Burkholderia Glyphosate AAR22262 3.00E−48 Prokaryotic ACA26961 0.004 1.4.99.1 1233 410 0 410 84 256 dehydrogenase xenovorans oxido- essential [Burkholderia LB400 reductase gene xenovorans gene #34740. LB400] downstream flanking region. 257, putative D- 146341475 1.00E−174 Bradyrhizo- Glyphosate AAR22262 3.00E−42 M. xanthus ACL70702 0.058 1.4.99.1 1233 410 0 412 70 258 amino-acid bium sp. oxido- protein dehydrogenase ORS278 reductase sequence, (DadA-like) gene seq id 9726. [Bradyrhizobium downstream sp. ORS278] flanking region. 259, D-amino acid 27377333 1.00E−163 Bradyrhizo- P. ADQ03060 1.00E−152 Human ABN17150 4.00E−12 1.4.99.1 1254 417 0 421 66 260 dehydrogenase bium aeruginosa ORFX small subunit japonicum virulence gene protein [Bradyrhizobium USDA 110 VIR14, protein. sequence japonicum SEQ ID USDA 110] NO:19716. 261, D-amino-acid 11871743 1.00E−166 Sinorhizo- Glyphosate AAR22262 2.00E−42 SigA2 AEB45551 3.6 1. . . 1248 415 0 417 65 262 dehydrogenase bium oxido- without [Sinorhizobium medicae reductase bla gene medicae WSM419 gene amplifying WSM419] downstream PCR primer, gi|113726415|gb| flanking SigA2- EAU07507.1| region. NotD-P, D-amino-acid SEQ ID dehydrogenase NO: 52. [Sinorhizobium medicae WSM419] 263, D-amino acid 13473406 1.00E−164 Mesorhizo- P. ADQ03060 1.00E−142 Rhizobium AAV30459 1.00E−12 1.4.99.1 1254 417 1257 418 66 67 264 dehydrogenase, bium aeruginosa species small subunit loti virulence gene symbiotic [Mesorhizobium VIR14, protein. plasmid loti]. pNGR234. 265, Methylenetetrahy 92114170 1.00E−112 Chromo- Bacterial ADS25020 2.00E−97 Bacterial ADS63414 7.00E−22 1.5.1.5 747 248 0 287 79 266 drofolate halobacter polypeptide polypeptide dehydrogenase salexigens #10001. #10001. (NADP+) DSM 3043 [Chromo- halobacter salexigens DSM 3043] 267, D-amino acid 33596520 1.00E−172 Bordetella P. ADQ03060 7.00E−95 Enterobacter AEH3102 0.001 1.4.99.1 1254 417 0 418 71 268 dehydrogenase parapertussis aeruginosa cloacae small subunit 12822 virulence gene protein [Bordetella VIR14, amino acid parapertussis protein. sequence - 12822] SEQ ID 5666. 269, D-amino acid 146280878 0 Pseudomonas P. ADQ03060 0 Pseudomonas ABD08815 3.00E−90 1.4.99.1 1299 432 0 432 78 270 dehydrogenase; stutzeri aeruginosa aeruginosa small subunit A1501 virulence gene polypeptide [Pseudomonas VIR14, #3. stutzeri A1501] protein. 271, D-amino-acid 73541345 1.00E−144 Ralstonia Glyphosate AAR22262 6.00E−46 Ramoplanin AAL40781 0.23 1.4.99.1 1239 412 0 414 59 272 dehydrogenase eutropha oxido- biosynthetic [Ralstonia JMP134 reductase ORF eutropha gene 20 protein. JMP134] downstream flanking region. 273, D-amino acid 21243478 1.00E−106 Xanthomonas P. ADQ03060 3.00E−47 A. orientalis ADY72597 0.23 1.4.99.1 1257 418 1251 416 48 60 274 dehydrogenase axonopodis aeruginosa polyene subunit pv. virulence polyketide [Xanthomonas citri str. 306 gene VIR14, ORF 14 axonopodis pv. protein. protein. citri str. 306]. 275, D-amino-acid 92118208 1.00E−174 Nitrobacter Glyphosate AAR22262 8.00E−54 M. xanthus ACL64753 0.92 1.4.99.1 1254 417 0 433 70 276 dehydrogenase hamburgensis oxido- protein [Nitrobacter X14 reductase sequence, hamburgensis gene seq id 9726. X14] downstream flanking region. 277, D-amino-acid 92118208 1.00E−170 Nitrobacter Glyphosate AAR22262 2.00E−56 Drosophila ABL23528 0.92 1.4.99.1 1254 417 0 433 69 278 dehydrogenase hamburgensis oxido- melanogaster [Nitrobacter X14 reductase polypeptide hamburgensis gene SEQ ID X14] downstream NO 24465. flanking region. 279, D-amino-acid 86750758 1.00E−116 Rhodo- Glyphosate AAR22262 1.00E−53 Bacterial ADS56371 3.6 1.4.99.1 1251 416 0 417 50 280 dehydrogenase pseudomonas oxido- polypeptide [Rhodo- palustris reductase #10001. pseudomonas HaA2 gene palustris downstream HaA2] flanking region. 281, D-amino-acid 121530396 1.00E−151 Ralstonia Glyphosate AAR22262 1.00E−45 Streptococcus AEC16041 0.058 1.4.99.1 1242 413 0 416 63 282 dehydrogenase pickettii 12J oxido- pyogenes [Ralstonia reductase protein pickettii 12J] gene G, SEQ gi|121302471|gb| downstream ID NO: 28. EAX43440.1| flanking region. 283, D-amino-acid 27377333 1.00E−158 Bradyrhizo- P. ADQ03060 1.00E−147 P. ADQ03059 4.00E−12 1.4.99.1 1263 420 0 421 62 284 dehydrogenase bium aeruginosa aeruginosa [Ralstonia japonicum virulence virulence pickettii 12J] USDA 110 gene VIR14, gene VIR14, D-amino acid protein. protein. dehydrogenase small subunit [Bradyrhizobium japonicum USDA 110] 285, D-amino acid 86141912 2.00E−87 Flavo- M. ADL05210 9.00E−53 Human ORF ABQ98347 3.7 1.4.99.1 1263 420 0 416 40 286 dehydrogenase bacterium catarrhalis DNA [Flavobacterium sp. MED217 protein #1. sequence #4. sp. MED217] 287, D-amino acid 87309573 1.00E−128 Blasto- Glyphosate AAR22262 3.00E−47 Drosophila ABL11756 0.92 1.4.99.1 1257 418 0 416 52 288 dehydrogenase, pirellula oxido- melanogaster small chain marina DSM reductase polypeptide [Blastopirellula 3645 gene SEQ ID marina DSM downstream NO 24465. 3645] flanking gi|87287337|gb|E region. AQ79237.1| D-amino acid dehydrogenase, small chain [Blastopirellula marina DSM 3645] 289, possible 39936835 1.00E−120 Rhodo- Glyphosate AAR22262 2.00E−52 M. xanthus ACL64803 0.06 1.4.99.1 1284 427 0 417 52 290 D-amino-acid pseudomonas oxido- protein dehydrogenase palustris reductase sequence, [Rhodopseudomo CGA009 gene seq id 9726. nas palustris downstream CGA009] flanking region. 291, D-amino acid 87309573 1.00E−136 Blasto- P. ADQ03060 3.00E−42 Enterobacter AEH54372 0.92 1.4.99.1 1263 420 0 416 56 292 dehydrogenase, pirellula aeruginosa cloacae small chain marina DSM virulence protein [Blastopirellula 3645 gene VIR14, amino acid marina DSM protein. sequence - 3645] SEQ ID gi|87287337|gb|E 5666. AQ79237.1| D- amino acid dehydrogenase, small chain [Blastopirellula marina DSM 3645] 293, D-amino acid 104781752 0 Pseudomonas Glyphosate AAR22262 1.00E−47 Arabidopsis ADT06724 0.23 1.4.99.1 1245 414 0 414 91 294 dehydrogenase; entomophila oxido- thaliana small subunit L48 reductase axidative family protein gene stress- [Pseudomonas downstream associated entomophila flanking protein #12. L48] region. 295, D-amino acid 124009931 4.00E−86 Microscilla Glyphosate AAR22262 5.00E−38 Microscilla AAV06555 0.24 1.4.99.1 1296 431 0 427 42 296 dehydrogenase marina ATCC oxido- furvescens small subunit, 23134 reductase catalase- putative gene 53CA1 [Microscilla downstream gene. marina ATCC flanking 23134] region. gi|123984082|gb| EAY24455.1| D-amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 297, D-amino acid 146284421 1.00E−177 Pseudomonas Acinetobacter ADA36279 1.00E−124 Acinetobacter ADA32153 3.6 1.4.99.1 1242 413 0 419 74 298 dehydrogenase; stutzeri baumannii baumannii small subunit A1501 protein protein [Pseudomonas #19. #19. stutzeri A1501] 299, D-amino acid 146284421 1.00E−176 Pseudomonas Acinetobacter ADA36279 1.00E−123 Acinetobacter ADA32153 3.6 1.4.99.1 1242 413 0 419 74 300 dehydrogenase; stutzeri baumannii baumannii small subunit A1501 protein protein [Pseudomonas #19. #19. stutzeri A1501] 301, putative d-amino 116250556 1.00E−168 Rhizobium Glyphosate AAR22262 3.00E−48 Pseudomonas ABD16228 0.92 1. . . 1254 417 0 415 66 302 acid legumino- oxidoreductase aeruginosa dehydrogenase sarum gene polypeptide small subunit bv. viciae downstream #3. [Rhizobium 3841 flanking leguminosarum region. bv. viciae 3841] 303, D-amino-acid 148553731 4.00E−90 Sphingomonas P. ADQ03060 3.00E−87 Enterobacter AEH53102 0.059 1.4.99.1 1269 422 0 416 42 304 dehydrogenase wittichii aeruginosa cloacae [Sphingomonas RW1 virulence protein wittichii RW1] gene VIR14, amino acid protein. sequence - SEQ ID 5666. 305, D-amino acid 26247503 0 Escherichia Enterobacter AEH60497 0 Enterobacter AEH53102 0 1.4.99.1 1299 432 0 434 93 306 dehydrogenase coli CFT073 cloacae cloacae small subunit protein protein [Escherichia coli amino acid amino acid CFT073] sequence - sequence - SEQ ID 5666. SEQ ID 5666. 307, putative d-amino 116250556 1.00E−129 Rhizobium Glyphosate AAR22262 9.00E−40 Aspergillus ADR84929 0.24 1. . . 1320 439 0 415 51 308 acid legumino- oxidoreductase fumigatus dehydrogenase sarum gene essential small subunit bv. viciae downstream gene [Rhizobium 3841 flanking protein #10. leguminosarum region. bv. viciae 3841] 309, D-amino acid 124009931 5.00E−98 Microscilla H. pylori AAW98270 9.00E−48 Human ADC87621 0.001 1.4.99.1 1242 413 0 427 44 310 dehydrogenase marina GHPO GPCR small subunit, ATCC 1099 gene. protein putative 23134 SEQ ID [Microscilla NO: 68. marina ATCC 23134] gi|123984082|gb| EAY24455.1| D- amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 311, D-amino acid 27377333 0 Bradyrhizo- P. ADQ03060 1.00E−150 Klebsiella ADB01189 3.00E−13 1.4.99.1 1266 421 0 421 95 312 dehydrogenase bium aeruginosa pneumoniae small subunit japonicum virulence polypeptide [Bradyrhizobium USDA 110 gene VIR14, seqid 7178. japonicum protein. USDA 110] 313, D-amino-acid 86750758 1.00E−119 Rhodo- Glyphosate AAR22262 3.00E−55 Bacterial ADS56475 3.6 1.4.99.1 1245 414 0 417 52 314 dehydrogenase pseudomonas oxidoreductase polypeptide [Rhodo- palustris gene #10001. pseudomonas HaA2 downstream palustris flanking HaA2] region. 315, D-amino-acid 73541345 1.00E−145 Ralstonia Glyphosate AAR22262 1.00E−45 Myco- AAI99682 0.058 1.4.99.1 1236 411 0 414 60 316 dehydrogenase eutropha oxidoreductase bacterium [Ralstonia JMP134 gene tuberculosis eutropha downstream strain JMP134] flanking H37Rv region. genome SEQ ID NO 2. 317, D-aminoacid 88712395 5.00E−96 Flavo- M. catarrhalis ADL05210 1.00E−43 Salmonella ADZ00150 0.91 1.4.99.1 1245 414 0 416 43 318 dehydrogenase bacteriales protein #1. typhi VexC [Flavobacteriales bacterium gene, reverse bacterium HTCC2170 PCR primer. HTCC2170] gi|88708933|gb|E AR01167.1] D- amino acid dehydrogenase [Flavobacteriales bacterium HTCC2170] 319, Enoyl-CoA 126727316 2.00E−96 Rhodo- Bacterial ADN25932 2.00E−77 Prokaryotic ACA26563 0.052 1.1.1.35 1110 369 0 646 47 320 hydra tase/ bacterales polypeptide essential gene isomerase:3- bacterium #10001. #34740. hydroxyacyl-CoA HTCC2150 dehydrogenase, 3-hydroxyacyl- CoA dehydrogenase, NAD-binding protein [Rhodobacterales bacterium HTCC2150] gi|126703311|gb| EBA02409.1| Enoyl-CoA hydratase/isomer ase:3- hydroxyacyl-CoA dehydrogenase, 3-hydroxyac 321, D-amino-acid 121530396 1.00E−170 Ralstonia Glyphosate AAR22262 1.00E−42 Prokaryotic ACA26625 0.004 1.4.99.1 1248 415 0 416 68 322 dehydrogenase pickettii 12J oxidoreductase essential gene [Ralstonia gene #34740. pickettii 12J] downstream gi|121302471|gb| flanking EAX43440.1| region. D-amino-acid dehydrogenase [Ralstonia pickettii 12J] 323, D-amino-acid 126355875 0 Pseudomonas Glyphosate AAR22262 2.00E−46 Bacterial ADS56305 0.015 1.4.99.1 1245 414 0 414 97 324 dehydrogenase putida GB-1 oxidoreductase polypeptide [Pseudomonas gene #10001. putida GB-1] downstream gi|126320385|gb| flanking EAZ71237.1| D- region. amino-acid dehydrogenase [Pseudomonas putida GB-1] 325, D-amino-acid 126356306 0 Pseudomonas P. ADQ03060 0 Pseudomonas ABD08815 ######## 1.4.99.1 1302 433 0 434 98 326 dehydrogenase putida GB-1 aeruginosa aeruginosa [Pseudomonas virulence polypeptide putida GB-1] gene VIR14, #3. gi|126319114|gb| protein. EAZ69967.1| D-amino-acid dehydrogenase [Pseudomonas putida GB-11 327, D-amino acid 21243478 1.00E−112 Xanthomonas P. ADQ03060 4.00E−47 Bacterial ADT47109 0.059 1.4.99.1 1254 417 1251 416 49 61 328 dehydrogenase axonopodis aeruginosa polypeptide subunit pv. virulence #10001. [Xanthomonas citri str. 306 gene VIR14, axonopodis pv. protein. citri str. 306]. 329, D-amino acid 88712395 4.00E−95 Flavo- H. pylori AAW98270 5.00E−46 Prokaryotic ACA32282 0.91 1.4.99.1 1251 416 0 416 43 330 dehydrogenase bacteriales GHPO essential gene [Flavobacteriales bacterium 1099 gene. #34740. bacterium HTCC2170 HTCC2170] gi|88708933|gb|E AR01167.1] D-amino acid dehydrogenase [Flavobacteriales bacterium 331, HTCC2170] 27377333 0 Bradyrhizo- P. ADQ03060 1.00E−144 Klebsiella ABD01189 1.00E−09 1.4.99.1 1256 421 0 421 79 332 D-amino acid bium aeruginosa pneumoniae dehydrogenase japonicum virulence polypeptide small subunit USDA 110 gene VIR14, seqid 7178. [Bradyrhizobium protein. japonicum USDA 110] 333, Enoyl-CoA 126727316 2.00E−96 Rhodo- Bacterial ADN25932 2.00E−77 Prokaryotic ACA26563 0.052 1.1.1.35 1110 369 0 648 47 334 hydratase/isomer bacterales polypeptide essential gene ase:3- bacterium #10001. #34740. hydroxyacyl-CoA HTCC2150 dehydrogenase, 3-hydroxyacyl- CoA dehydrogenase, NAD-binding protein [Rhodobacterales bacterium HTCC2150] gi|126703311|gb| EBA02409.1| Enoyl-CoA hydratase/isomer ase:3- hydroxyacyl-CoA dehydrogenase, 3-hydroxyac 335, D-amino-acid 121530396 1.00E−170 Ralstonia Glyphosate AAR22262 1.00E−42 Prokaryotic ACA26625 0.004 1.4.99.1 1248 415 0 416 68 336 dehydrogenase pickettii 12J oxidoreductase essential gene [Ralstonia gene #34740. pickettii 12J] downstream gi|121302471|gb| flanking EAX43440.1| region. D-amino-acid dehydrogenase [Ralstonia pickettii 12J] 337, D-amino-acid 86750758 1.00E−114 Rhodo- Glyphosate AAR22262 1.00E−50 Thale cress AEI59268 0.91 1.4.99.1 1248 415 0 417 49 338 dehydrogenase pseudomonas oxidoreductase polypeptide, [Rhodo- palustris gene SEQ ID pseudomonas HaA2 downstream NO: 32. palustris flanking HaA2] region. 339, D-amino acid 124009931 1.00E−100 Microscilla M. catarrhalis ADL05210 7.00E−47 Mouse ADC85461 0.91 1.4.99.1 1245 414 0 427 42 340 dehydrogenase marina ATCC protein #1. Tnfrsf6 small subunit, 23134 genomic putative sequence. [Microscilla marina ATCC 23134] gi|123984082|gb| EAY24455.1| D-amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 341, D-amino-acid 121530396 1.00E−170 Ralstonia Glyphosate AAR22262 2.00E−42 Prokaryotic ACA26625 0.91 1.4.99.1 1248 415 0 416 68 342 dehydrogenase pickettii 12J oxidoreductase essential gene [Ralstonia gene #34740. pickettii 12J] downstream gi|121302471|gb| flanking EAX43440.1| region. D-amino-acid dehydrogenase [Ralstonia pickettii 12J] 343, short-chain 146275754 2.00E−51 Novos- Bacterial ADS24609 5.00E−26 Bacterial ADS58499 0.008 1.1.1.184 693 230 0 228 48 344 dehydrogenase/ phingobium polypeptide polypeptide reductase SDR aromatici- #10001. #10001. [Novos- vorans phingobium DSM 12444 aromaticivorans DSM 12444] 345, D-amino-acid 114570652 2.00E−91 Maricaulis P. ADQ03060 4.00E−78 Human ABN17150 4.00E−09 1.4.99.1 1260 419 0 427 43 346 dehydrogenase maris MCS10 aeruginosa ORFX [Maricaulis virulence protein maris MCS10] gene VIR14, sequence gi|114341114|gb| protein. SEQ ID ABI66394.1| D- NO: 19716. amino-acid dehydrogenase [Maricaulis maris MCS1Q] 347, D-amino-acid 113871743 1.00E−152 Sinorhizobium Glyphosate AAR22262 1.00E−48 M. xanthus ACL64399 0.23 1. . . 1245 414 0 417 63 348 dehydrogenase medicae oxidoreductase protein [Sinorhizobium WSM419 gene sequence, medicae downstream seq id 9726. WSM419] flanking gi|113726415|gb| region. EAU07507.1| D- amino-acid dehydrogenase [Sinorhizobium medicae WSM419] 349, D-amino-acid 86750758 1.00E−117 Rhodo- Glyphosate AAR22262 3.00E−55 M. xanthus ACL64798 0.91 1.4.99.1 1251 416 0 417 51 350 dehydrogenase pseudomonas oxidoreductase protein [Rhodo- palustris gene sequence, pseudomonas HaA2 downstream seq id 9726. palustris flanking HaA2] region. 351, D-amino acid 32473614 1.00E−155 Rhodo- N. ABP80542 2.00E−48 Plant ADT17628 0.92 1.4.99.1 1260 419 0 456 61 352 dehydrogenase; pirellula gonorrhoeae polypeptide, small chain baltica SH 1 nucleotide SEQ ID [Rhodopirellula sequence SEQ 5546. baltica SH 1] ID 4691. 353, D-amino acid 88712395 1.00E−88 Flavo- Bacterial ADF07894 9.00E−53 Sorangium AED50859 0.059 1.4.99.1 1263 420 0 416 40 354 dehydrogenase bacteriales polypeptide cellulosum [Flavobacteriales bacterium #19. jerangolid bacterium HTCC2170 biosynthetic HTCC2170] cluster gi|88708933|gb|E jerB protein. AR01167.1] D- amino acid dehydrogenase [Flavobacteriales bacterium HTCC21701 355, aldehyde 108761092 2.00E−97 Myxococcus Bacterial ADN25785 1.00E−87 Bacterial ADS56451 8.00E−07 1.2.1.39 1020 339 0 524 55 356 dehydrogenase xanthus DK polypeptide polypeptide family protein 1622 #10001. #10001. [Myxococcus xanthus DK 1622] 357, D-amino acid 124009931 1.00E−96 Microscilla M. ADL05210 1.00E−42 Human ABD32968 0.91 1.4.99.1 1251 416 0 427 40 358 dehydrogenase marina ATCC catarrthalis cancer- small subunit, 23134 protein #1. associated putative protein [Microscilla HP13-036.1. marina ATCC 23134] gi|123984082|gb| EAY24455.1| D-amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 359, D-amino-acid 111019145 1.00E−163 Rhodococcus C AAG93079 1.00E−103 Klebsiella ABD00632 0.23 1.4.99.1 1251 416 0 415 68 360 dehydrogenase sp. RHA1 glutamicum pneumoniae small subunit coding polypeptide [Rhodococcus sequence seqid 7178. sp. RHA1] fragment SEQ ID NO: 1935. 361, D-amino-acid 111019145 1.00E−163 Rhodococcus C AAG93079 1.00E−103 Klebsiella ABD00632 0.23 1.4.99.1 1251 416 0 415 68 362 dehydrogenase sp. RHA1 glutamicum pneumoniae small subunit coding polypeptide [Rhodococcus sequence seqid 7178. sp. RHA1] fragment SEQ ID NO: 1935. 363, D-amino-acid 111019145 1.00E−163 Rhodococcus C AAG93079 1.00E−103 Klebsiella ABD00632 0.23 1.4.99.1 1251 416 0 415 68 364 dehydrogenase sp. RHA1 glutamicum pneumoniate small subunit coding polypeptide [Rhodococcus sequence seqid 7178. sp. RHA1] fragment SEQ ID NO: 1935. 365, D-amino acid 27377333 0 Bradyrhizo- P. ADQ03060 1.00E−149 Klebsiella ABD01189 2.00E−11 1.4.99.1 1266 421 0 421 93 366 dehydrogenase bium aeruginosa pneumoniate small subunit japonicum virulence polypeptide [Bradyrhizobium USDA 110 gene VIR14, seqid 7178. japonicum protein. USDA 110] 367, D-amino acid 134093986 0 Herminii- P. ADQ03060 1.00E−101 Klebsiella ABD01189 3.00E−07 1.4.99.1 1317 438 0 443 80 368 dehydrogenase monas aeruginosa pneumoniae small subunit arsenico- virulence polypeptide [Herminiimonas xydans gene VIR14, seqid 7178. arsenicoxydans] protein. gi|133737889|em b|CAL60934.1| D-amino acid dehydrogenase small subunit [Herminiimonas arsenicoxydans] 369, D-amino acid 124009931 1.00E−101 Microscilla H. pylori AAW98270 1.00E−148 Soybean AEI27664 0.23 1.4.99.1 1245 414 0 427 44 370 dehydrogenase marina ATCC GHPO polymorphic small subunit, 23134 1099 gene. locus, putative SEQ ID 6. [Microscilla marina ATCC 23134] gi|123984082|gb| EAY24455.1| D-amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 371, D-amino acid 27377333 0 Bradyrhizo- P. ADQ03060 1.00E−148 Rhizobium AAV30459 1.00E−15 1.4.99.1 1266 421 0 421 94 372 dehydrogenase bium aeruginosa species small subunil japonicum virulence symbiotic [Bradyrhizobium USDA 110 gene VIR14, plasmid japonicum protein. pNGR234. USDA 110] 373, D-amino-acid 73541345 1.00E−136 Ralstonia Glyphosate AAR22262 3.00E−47 Bacterial ADS56859 0.23 1.4.99.1 1260 419 0 414 57 374 dehydrogenase eutropha oxidoreductase polypeptide [Ralstonia JMP134 gene #10001. eutropha downstream JMP134] flanking region. 375, D-amino acid 91217360 3.00E−98 Psychroflexus Acinetobacter ADA33588 2.00E−47 Cyclin- ADX06332 3.6 1.4.99.1 1257 418 0 415 43 376 dehydrogenase torquis ATCC baumannii dependent [Psychroflexus 700755 protein kinase torquis ATCC #19. modulation 700755] biomarker gi|91184468|gb|E SEQ ID AS70851.1| D- NO 24. amino acid dehydrogenase [Psychroflexus torquis ATCC 700755] 377, D-amino acid 88712395 1.00E−122 Flavo- M. ADL05210 4.00E−40 Human AAK69489 0.23 1.4.99.1 1245 414 0 416 51 378 dehydrogenase bacteriales catarrthalis immune/ [Flavobacteriales bacterium protein #1. haemato- bacterium HTCC2170 poietic HTCC2170] antigen gi|88708933|gb|E genomic AR01167.1| D- sequence amino acid SEQ ID dehydrogenase NO: 41436. [Flavobacteriales bacterium HTCC2170] 379, D-amino-acid 114570652 8.00E−91 Maricaulis P. ADQ03060 6.00E−78 B. AED48875 0.015 1.4.99.1 1257 418 0 427 43 380 dehydrogenase maris MCS10 aeruginosa circulans [Maricaulis virulence putative maris MCS10] gene VIR14, CapJ gi|114341114|gb| protein. protein. ABI66394.1| D- amino-acid dehydrogenase [Maricaulis maris MCS1Q] 381, D-amino-acid 111019145 1.00E−163 Rhodococcus C AAG93079 1.00E−103 Klebsiella ABD00632 0.23 1.4.99.1 1251 416 0 415 68 382 dehydrogenase sp. RHA1 glutamicum pneumoniae small subunit coding polypeptide [Rhodococcus sequence seqid 7178. sp. RHA1] fragment SEQ ID NO: 1935. 383, D-amino acid 13473406 0 Mesorhizo- P. ADQ03060 1.00E−140 P. ADQ03059 7.00E−11 1.4.99.1 1260 419 1257 418 88 87 384 dehydrogenase, bium aeruginosa aeruginosa small subunit loti virulence virulence [Mesorhizobium gene VIR14, gene VIR14, loti]. protein. protein. 385, D-amino acid 124009931 5.00E−99 Microscilla H. pylori AAW98270 1.00E−47 Human ABA19863 0.91 1.4.99.1 1245 414 0 427 42 386 dehydrogenase marina ATCC GHPO nervous small subunit, 23134 1099 gene. system putative related [Microscilla poly- marina ATCC nucleotide 23134] SEQ ID NO gi|123984082|gb| 11589. EAY24455.1| D-amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 387, D-amino acid 13474742 0 Mesorhizo- Pseudomonas ABO75104 2.00E−90 M. AAZ19073 0.91 1.4.99.1 1251 416 1251 416 75 74 388 dehydrogenase, bium aeruginosa tuberculosis small subunit loti polypeptide recombinant [Mesorhizobium #3. antigen DNA loti]. encoding 3′ XP32 389, D-amino acid 134093986 0 Herminii- Klebsiella ABO67618 2.00E−95 Klebsiella ABD01189 2.00E−08 1.4.99.1 1317 438 0 443 80 390 dehydrogenase monas pneumoniae pneumoniae small subunit arsenico- polypeptide polypeptide [Herminiimonas xydans seqid 7178. seqid 7178. arsenicoxydans] gi|133737889|em b|CAL60934.1| D-amino acid dehydrogenase small subunit [Herminiimonas arsenicoxydans] 87309573 1.00E−139 Blasto- N. ABP80542 1.00E−44 Myco- ADB80216 0.92 1.4.99.1 1257 418 0 416 55 391, D-amino acid pirellula gonorrhoeae bacterium 392 dehydrogenase, marina DSM nucleotide tuberculosis small chain 3645 sequence SEQ nitrient [Blastopirellula ID 4691. starvation- marina DSM inducible 3645] protein #8. gi|87287337|gb|E AQ79237.1| D-amino acid dehydrogenase, small chain [Blastopirellula marina DSM 3645] 393, probable D- 119897258 1.00E−111 Azoarcus sp. Pseudomonas ABO84309 1.00E−74 Enterobacter AEH53102 0.001 1.4.99.1 1257 418 0 416 51 394 amino acid BH72 aeruginosa cloacae dehydrogenase polypeptide protein small subunit #3. amino acid [Azoarcus sp. sequence - BH72] SEQ ID 5666. 395, D-amino-acid 73541345 0 Ralstonia Glyphosate AAR22262 1.00E−45 Plant full ADX30055 0.058 1.4.99.1 1242 413 0 414 88 396 dehydrogenase eutropha oxidoreductase length [Ralstonia JMP134 gene insert eutropha downstream polynuceotide JMP134] flanking seqid 4980. region. 397, D-aminoacid 86750758 1.00E−121 Rhodo- Glyphosate AAR22262 1.00E−56 Bacterial ADT43936 0.015 1.4.99.1 1245 414 0 417 52 398 dehydrogenase pseudomonas oxidoreductase polypeptide [Rhodo- palustris gene #10001. pseudomonas HaA2 downstream palustris flanking HaA2] region. 399, D-amino acid 124009931 2.00E−97 Microscilla H. pylori AAW98270 1.00E−45 Human ADL62778 0.23 1.4.99.1 1242 413 0 427 44 400 dehydrogenase marina ATCC GHPO ovarian small subunit, 23134 1099 gene. cancer DNA putative marker #5. [Microscilla marina ATCC 23134] gi|123984082|gb| EAY24455.1] D-amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 401, D-amino-acid 114570652 3.00E−66 Maricaulis M. ADL05210 9.00E−56 A. thaliana AAD06652 3.6 1.4.99.1 1250 419 0 427 33 402 dehydrogenase maris catarrthalis transcription [Maricaulis MCS1Q protein #1. factor maris MCS10] G207 gi|114341114|gb| homolog, ABI66394.1| G227 cDNA. D-amino-acid dehydrogenase [Maricaulis maris MCS1Q] 403, D-amino acid 124009931 1.00E−111 Microscilla H. pylori AAW98270 3.00E−46 A. ABQ80343 3.6 1.4.99.1 1257 418 0 427 47 404 dehydrogenase marina ATCC GHPO fumigatus small subunit, 23134 1099 gene. AfGOX3. putative [Microscilla marina ATCC 23134] gi|123984082|gb| EAY24455.1| D-amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 405, D-amino acid 88806240 2.00E−95 Robiginitalea Glyphosate AAR22262 3.00E−45 Human 7a5 ADW91994 0.91 1.4.99.1 1239 412 0 417 42 406 dehydrogenase biformata oxidoreductase prognostin [Robiginitalea HTCC2501 gene protein biformata downstream sequence HTCC2501] flanking SeqID2. gi|88783849|gb|E region. AR15020.1| 407, D-amino acid 27377333 0 Bradyrhizo- P. ADQ03060 1.00E−149 Klebsiella ABD01189 2.00E−11 1.4.99.1 1266 421 0 421 94 408 dehydrogenase bium aeruginosa pneumoniae [Robiginitalea japonicum virulence polypeptide biformata USDA 110 gene VIR14, seqid 7178. HTCC2501] protein. D-amino acid dehydrogenase small subunit [Bradyrhizobium japonicum USDA 110] 409, D-amino acid 124009931 2.00E−98 Microscilla P. ADQ03060 4.00E−52 Human soft ADQ18897 0.92 1.4.99.1 1260 419 0 427 45 410 dehydrogenase marina ATCC aeruginosa tissue small subunit, 23134 virulence sarcoma- putative gene VIR14, upregulated [Microscilla protein. protein - marina ATCC SEQ ID 40. 23134] gi|123984082|gb| EAY24455.1| D-amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 411, ketoglutarate 104783034 1.00E−103 Pseudomonas Bacterial ADN25785 1.00E−103 Bacterial ADS55665 4.00E−09 1.2.1.4 1242 413 0 526 50 412 semialdehyde entomophila polypeptide polypeptide dehydrogenase L48 #10001. #10001. [Pseudomonas entomophila L48] 413, D-amino-acid 86750758 1.00E−119 Rhodo- Glyphosate AAR22262 3.00E−57 Prokaryotic ACA25332 0.23 1.4.99.1 1248 415 0 417 52 414 dehydrogenase pseudomonas oxidoreductase essential gene [Rhodo- palustris gene #34740. pseudomonas HaA2 downstream palustris flanking HaA2] region. 415, D-amino-acid 86750758 1.00E−125 Rhodo- Glyphosate AAR22262 1.00E−47 Drosophila ABL20166 0.015 1.4.99.1 1242 413 0 417 53 416 dehydrogenase pseudomonas oxidoreductase melanogaster [Rhodo- palustris gene polypeptide pseudomonas HaA2 downstream SEQ ID palustris flanking NO 24465. HaA2] region. 417, AGR_L_3050p 15891640 1.00E−179 Agro- P. ADQ03060 1.00E−150 E. coli ACD81455 3.00E−10 1.4.99.1 1248 415 1257 418 73 73 418 [Agrobacterium bacterium aeruginosa K12 MG1655 tumefaciens]. tumefaciens virulence biochip gene VIR14, probe protein. SEQ ID 1. 419, D-amino-acid 91786059 0 Polaromonas Photorhabdus ABM69115 1.00E−84 P. ADQ03059 2.00E−05 1.4.99.1 1326 441 0 445 76 420 dehydrogenase sp. JS666 luminescens aeruginosa [Polaromonas protein virulence sp. JS666] sequence gene VIR14, #59. protein. 421, D-amino-acid 108804652 1.00E−108 Rubrobacter C AAG93079 8.00E−81 Prokaryotic ACA37735 0.24 1.4.99.1 1293 430 0 419 49 422 dehydrogenase xylanophilus glutamicum essential gene [Rubrobacter DSM 9941 coding #34740. xylanophilus sequence DSM 9941] fragment SEQ ID NO: 1935. 423, D-amino acid 124009931 4.00E−94 Microscilla H. pylori AAW98270 4.00E−44 Chemically ABL70446 0.91 1.4.99.1 1245 414 0 427 43 424 dehydrogenase marina ATCC GHPO treated small subunit, 23134 1099 gene. cell putative signalling [Microscilla DNA marina ATCC sequence#234. 23134] gi|123984082|gb| EAY24455.1| D-amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 425, D-amino-acid 121530396 1.00E−122 Ralstonia Glyphosate AAR22262 2.00E−44 Plant ADJ44795 0.92 1.4.99.1 1260 419 0 416 53 426 dehydrogenase pickettii 12J oxidoreductase cDNA #31. [Ralstonia gene pickettii 12J] downstream gi|121302471|gb| flanking EAX43440.1| region. D-amino-acid dehydrogenase [Ralstonia pickettii 12J] 427, D-amino-acid 92118208 0 Nitrobacter Glyphosate AAR22262 2.00E−49 Bacterial ADS57588 0.92 1.4.99.1 1254 417 0 433 72 428 dehydrogenase hamburgensis oxidoreductase polypeptide [Nitrobacter X14 gene #10001. hamburgensis downstream X14] flanking region. 429, D-amino acid 124009931 2.00E−97 Microscilla Glyphosate AAR22262 4.00E−48 Haemophilus AAT42063 0.059 1.4.99.1 1263 420 0 427 44 430 dehydrogenase marina ATCC oxidoreductase influenzae small subunit, 23134 gene complete putative downstream genome [Microscilla flanking sequence. marina ATCC region. 23134] gi|123984082|gb| EAY24455.1| D-amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 431, D-amino acid 124009931 1.00E−119 Microscilla Glyphosate AAR22262 2.00E−49 Lactic ADF77343 0.91 1.4.99.1 1242 413 0 427 49 432 dehydrogenase marina ATCC oxidoreductase acid small subunit, 23134 gene bacteria putative downstream Lactobacillus [Microscilla flanking johnsonii marina ATCC region. La1 23134] genomic gi|123984082|gb| DNA EAY24455.1| SEQ ID D-amino acid NO: 1. dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 433, D-amino-acid 86750758 1.00E−123 Rhodo Glyphosate AAR22262 4.00E−45 Drosophila AEF68383 0.23 1.4.99.1 1245 414 0 417 52 434 dehydrogenase pseudomonas oxidoreductase melanogaster [Rhodo paluslris gene modified pseudomonas HaA2 downstream translational paluslris flanking start HaA2] region. site DNA. 435, D-amino-acid 86750758 1.00E−121 Rhodo- Glyphosate AAR22262 3.00E−41 Human ADB80390 0.92 1.4.99.1 1254 417 0 417 51 436 dehydrogenase pseudomonas oxidoreductase MDDT [Rhodo- palustris gene protein SEQ pseudomonas HaA2 downstream ID NO: 2. palustris flanking HaA2] region. 437, carbon monoxide 56697247 1.00E−66 Silicibacter Bacterial ADS27598 0.12 Rice abiotic ACL28407 1.2 1.2.99.2 453 150 0 150 84 438 dehydrogenase pomeroyi polypeptide stress G protein; DSS-3 #10001. responsive putative polypeptide [Silicibacter SEQ ID pomeroyi NO: 4152. DSS-3] 439, carbon monoxide 56697248 1.00E−87 Silicibacter DNA ABG25235 9.00E−09 Bacterial ADS63732 2.2 780 259 0 260 64 440 dehydrogenase pomeroyi encoding polypeptide F protein DSS-3 novel #10001. [Silicibacter human pomeroyi diagnostic DSS-3] protein #20574. 441, carbon monoxide 56697249 1.00E−171 Silicibacter Human AAU75888 3.00E−25 Neisseria AEB49411 0.22 1182 393 0 393 76 442 dehydrogenase pomeroyi adhesion PCR E protein DSS-3 molecule primer SEQ [Silicibacter protein ID NO 1078. pomeroyi AD6/ DSS-3] CAA17374.11 443, D-amino-acid 113871743 1.00E−150 Sinorhizo- Glyphosate AAR22262 4.00E−55 Prokaryotic ACA26463 0.06 1. . . 1272 423 0 417 61 444 dehydrogenase bium oxidoreductase essential gene [Sinorhizobium medicae gene #34740. medicae WSM419 downstream WSM419] flanking gi|113726415|gb| region. EAU07507.1| 445, D-amino-acid 126646463 1.00E−175 Algoriphagus H. pylon AAW98270 1.00E−45 Tp1 peptide AEK18770 0.015 1.4.99.1 1245 414 0 415 69 446 dehydrogenase sp. PR1 GHPO fragment [Sinorhizobium 1099 gene. SEQ ID medicae NO: 78. WSM419] D-amino acid dehydrogenase [Algoriphagus sp. PR1] gi|126578095|gb| EAZ82315.1| D-amino acid dehydrogenase [Algoriphagus sp. PR1] 447, D-amino-acid 119877440 0 Steno- P. ADQ03060 0 Pseudomonas ABD08815 4.00E−37 1.4.99.1 1308 435 0 434 84 448 dehydrogenase trophomonas aeruginosa aeruginosa [Steno- maltophilia virulence polypeptide trophomonas R551-3 gene VIR14, #3. maltophilia protein. R551-3] gi|119820020|gb| EAX22641.1| D-amino-acid dehydrogenase [Steno- trophomonas maltophilia R551-3] 449, D-amino-acid 121530396 1.00E−104 Ralstonia Glyphosate AAR22262 9.00E−37 Pseudomonas ABD03414 0.015 1.4.99.1 1248 415 0 416 48 450 dehydrogenase pickettii 12J oxidoreductase aeruginosa [Ralstonia gene polypeptide pickettii 12J] downstream #3. gi|121302471|gb| flanking EAX43440.1| region. D-amino-acid dehydrogenase [Ralstonia pickettii 12J] 451, D-amino-acid 121530396 1.00E−143 Ralstonia Glyphosate AAR22262 2.00E−49 Prokaryotic ACA23380 0.23 1. . . 1245 414 0 416 62 452 dehydrogenase pickettii 12J oxidoreductase essential gene [Ralstonia gene #34740. pickettii 12J] downstream gi|121302471 |gb| flanking EAX43440.1| region. D-amino-acid dehydrogenase [Ralstonia pickettii 12J] 453, D-amino-acid 113871743 1.00E−125 Sinorhizo- Glyphosate AAR22262 9.00E−37 Human ABN24675 0.92 1. . . 1254 417 0 417 53 454 dehydrogenase bium oxidoreductase ORFX [Sinorhizobium medicae gene protein medicae WSM419 downstream sequence WSM419] flanking SEQ ID gi|113726415|gb| region. NO: 19716. EAU07507.1| D-amino-acid dehydrogenase [Sinorhizobium medicae WSM419] 455, D-amino acid 124009931 1.00E−104 Microscilla H. pylori AAW98270 2.00E−50 Human ACA64961 0.91 1.4.99.1 1242 413 0 427 45 456 dehydrogenase marina ATCC GHPO IMAGE small subunit, 23134 1099 gene. 249058 putative DNA [Microscilla corresponding marina ATCC to 23134] AW006742. gi|123984082|gb| EAY24455.1| D-amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 457, D-amino acid 88712395 1.00E−173 Flavo- M. ADL05210 2.00E−48 Drosophila ABL10568 0.23 1.4.99.1 1251 416 0 416 68 458 dehydrogenase bacteriales catarrhalis melanogaster [Flavobacteriales bacterium protein #1. polypeptide bacterium HTCC2170 SEQ ID HTCC2170) NO 24465. gi|88708933|gb|E AR01167.11 D-amino acid dehydrogenase [Flavobacteriales bacterium HTCC2170] 459, D-amino acid 126666221 0 Marinobacter Acinetobacter ADA36279 1.00E−129 Drosophila ABL12550 3.7 1.4.99.1 1266 421 0 410 78 460 dehydrogenase sp. ELB17 baumannii melanogaster small subunit protein polypeptide [Marinobacter #19. SEQ ID sp. ELB17] NO 24465. gi|126629543|gb| EBA00161.1| D-amino acid dehydrogenase small subunit [Marinobacter sp. ELB17] 461, Short-chain 67154056 1.00E−109 Azotobacter Klebsiella ABO66363 1.00E−104 Klebsiella ACH99914 6.00E−04 1. . . 762 253 0 254 76 462 dehydrogenase/ vinelandii pneumoniae pneumoniae reductase SDR AvOP polypeptide polypeptide [Azotobacter seqid 7178. seqid 7178. vinelandii AvOP] 463, D-amino acid 91217360 1.00E−167 Psychroflexus H. pylori AAW98270 2.00E−52 Novel ADQ64455 0.23 1.4.99.1 1248 415 0 415 69 464 dehydrogenase torquis ATCC GHPO human [Psychroflexus 700755 1099 gene. protein torquis ATCC sequence #4. 700755] gi|91184468|gb|E AS70851.1] D-amino acid 465, dehydrogenase 56420489 4.00E−18 Geobacillus Hyper- ADM25691 6.00E−11 AB005287 AAC90078 8.00E−05 1.1.1.95 447 148 0 334 34 466 [Psychroflexus kaustophilus thermophile cDNA torquis ATCC HTA426 Methano- clone. 700755] pyrus dehydrogenase kandleri [Geobacillus protein #28. kaustophilus HTA426] 467, D-amino-acid 73541345 1.00E−133 Ralstonia Glyphosate AAR22262 1.00E−38 Thale cress AEI59858 0.23 1.4.99.1 1242 413 0 414 55 468 dehydrogenase eutropha oxidoreductase polypeptide, [Ralstonia JMP134 gene SEQ ID eutropha downstream NO: 32. JMP134] flanking region. 469, putative 116250556 1.00E−166 Rhizobium Glyphosate AAR22262 5.00E−46 Murine ADZ13449 0.91 1. . . 1245 414 0 415 66 470 d-amino acid legurnino- oxidoreductase cancer- dehydrogenase sarum gene associated small subunit bv. viciae downstream genomic [Rhizobium 3841 flanking DNA #5. legurninosarum region. bv. viciae 3841] 471, D-amino acid 26247503 0 Escherichia Enterobacter AEH60497 0 DNA AAS77111 0 1.4.99.1 1299 432 0 434 100 472 dehydrogenase coli cloacae encoding small subunit CFT073 protein novel [Escherichia coli amino acid human CFT073] sequence - diagnostic SEQ ID 5666. protein #20574. 473, D-amino-acid 113871743 1.00E−159 Sinorhizo- Glyphosate AAR22262 3.00E−47 G sorghi ACF04822 0.91 1. . . 1248 415 0 417 64 474 dehydrogenase bium oxidoreductase nitrilase [Sinorhizobium medicae gene protein medicae WSM419 downstream fragment #2. WSM419) flanking gi|113726415|gb| region. EAU07507.1| D-amino-acid dehydrogenase [Sinorhizobium medicae WSM419] 475, D-amino-acid 114570652 1.00E−155 Maricaulis P. ADQ03060 4.00E−75 S ADN97550 0.25 1.4.99.1 1341 446 0 427 62 476 dehydrogenase maris aeruginosa ambofaciens [Maricaulis MCS10 virulence spiramycin maris MCS10] gene VIR14, biosynthetic gi|114341114|gb| protein. enzyme ABI66394.1| encoded D-amino-acid by ORF10*. dehydrogenase [Maricaulis maris MCS10] 477, D-amino acid 88712395 4.00E−85 Flavo- P. ADQ03060 3.00E−51 Human ACN44608 0.001 1.4.99.1 1239 412 0 416 38 478 dehydrogenase bacteriales aeruginosa protein [Flavobacteriales bacterium virulence sequence bacterium HTCC2170 gene VIR14, hCP39072. HTCC2170] protein. gi|88708933|gb|E AR01167.11 D-amino acid dehydrogenase [Flavobacteriales bacterium HTCC2170] 479, D-amino acid 88712395 4.00E−85 Flavo- P. ADQ03060 3.00E−51 Human ACN44608 0.001 1.4.99.1 1239 412 0 416 38 480 dehydrogenase bacteriales aeruginosa protein [Flavobacteriales bacterium virulence sequence bacterium HTCC2170 gene VIR14, hCP39072. HTCC2170] protein. gi|88708933|gb|E AR01167.11 D-amino acid dehydrogenase [Flavobacteriales bacterium HTCC2170] 481, D-amino acid 88712395 1.00E−175 Flavo- M. ADL05210 3.00E−50 Human ADF69167 0.059 1.4.99.1 1251 416 0 416 69 482 dehydrogenase bacteriales catarrhalis MP53 [Flavobacteriales bacterium protein #1. protein bacterium HTCC2170 sequence HTCC2170] SEQ ID gi|88708933|gb|E NO: 69. AR01167.1] D-amino acid dehydrogenase [Flavobacteriales bacterium HTCC2170] 483, D-amino acid 134093986 0 Herminii- P. ADQ03060 4.00E−94 Pseudomonas ABD08815 0.001 1.4.99.1 1311 436 0 443 82 484 dehydrogenase monas aeruginosa aeruginosa small subunit arsenicoxy- virulence polypeptide [Herminiimonas dans gene VIR14, #3. arsenicoxydans] protein. gi|133737889|em b|CAL60934.1| 485, D-amino acid 27377333 1.00E−167 Bradyrhizo- P. ADQ03060 1.00E−154 Human ABN17150 2.00E−11 1.4.99.1 1269 422 0 421 67 486 dehydrogenase bium aeruginosa ORFX small subunit japonicum virulence protein [Herminiimonas USDA 110 gene VIR14, sequence arsenicoxydans] protein. SEQ ID D-amino acid NO: 19716. dehydrogenase small subunit [Bradyrhizobium japonicum USDA 110] 487 D-amino-acid 113871743 1.00E−129 Sinorhizo- Glyphosate AAR22262 1.00E−35 M. ADI37347 3.7 1. . . 1263 420 0 417 53 488 dehydrogenase bium oxidoreductase tuberculosis [Sinorhizobium medicae gene low oxygen medicae WSM419 downstream induced WSM419] flanking antigen gi|113726415|gb| region. Rv0363c EAU07507.1| SEQ ID D-amino-acid NO: 4. dehydrogenase [Sinorhizobium medicae WSM419] 489, D-amino-acid 115523700 1.00E−123 Rhodo- Glyphosate AAR22262 5.00E−49 Bacterial ADS57628 0.91 1.4.99.1 1242 413 0 417 54 490 dehydrogenase pseudomonas oxidoreductase polypeptide [Rhodo- paluslris gene #10001. pseudomonas BisA53 downstream paluslris flanking BisA53] region. 491, D-amino-acid 114570652 1.00E−78 Maricaulis P. ADQ03060 5.00E−59 Prokaryotic ACA26589 0.015 1.4.99.1 1290 429 0 427 41 492 dehydrogenase maris aeruginosa essential gene [Maricaulis MCS10 virulence #34740. maris MCS10] gene VIR14, gi|114341114|gb| protein. ABI66394.1| D-amino-acid dehydrogenase [Maricaulis maris MCS10] 493, D-amino acid 124009931 6.00E−90 Microscilla C AAG93079 2.00E−37 Plan full ADO81756 0.063 1.4.99.1 1344 447 0 427 41 494 dehydrogenase marina ATCC glutamicum length small subunit, 23134 coding insert putative sequence polynucleotide [Microscilla fragment seqid 4980. marina ATCC SEQ ID 23134] NO: 1935. gi|123984082|gb| EAY24455.1| D-amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 495, D-amino-acid 115523700 1.00E−112 Rhodo- Glyphosate AAR22262 2.00E−54 Plan full ADO81304 0.92 1.4.99.1 1254 417 0 417 49 496 dehydrogenase pseudomonas oxidoreductase length [Rhodo- palustris gene insert pseudomonas BisA53 downstream polynucleotide palustris flanking seqid 4980. BisA53] region. 497, D-amino-acid 113871743 1.00E−129 Sinorhizo- Glyphosate AAR22262 1.00E−35 Drosophila ABL27820 3.7 1. . . 1263 420 0 417 53 498 dehydrogenase bium oxidoreductase melanogaster [Sinorhizobium medicae gene polypeptide medicae WSM419 downstream SEQ ID WSM419] flanking NO 24465. gi|113726415|gb| region. EAU07507.1| D-amino-acid dehydrogenase [Sinorhizobium medicae WSM419] 499, D-amino-acid 115523700 1.00E−112 Rhodo- Glyphosate AAR22262 2.00E−36 Human AAL04340 0.91 1.4.99.1 1245 414 0 417 49 500 dehydrogenase pseudomonas oxidoreductase reproductive [Rhodo- palustris gene system related pseudomonas BisA53 downstream antigen DNA palustris flanking SEQ ID BisA53] region. NO: 8114. 501, delta-1-pyrroline- 68535524 1.00E−174 Coryne- Coryne- AAB79787 1.00E−170 Coryne- AAF71906 0.11 1.5.1.12 2334 777 0 1158 48 502 5-carboxylate bacterium bacterium bacterium dehydrogenase jeikeium glutamicum glutamicum [Corynebacterium K411] MP MP jeikeium K411] protein protein sequence sequence SEQ ID SEQ ID NO: 1148. NO: 1148. 503, D-amino acid 87309573 1.00E−129 Blasto- C AAG93079 8.00E−44 Bacterial ADS59567 0.015 1.4.99.1 1263 420 0 416 49 504 dehydrogenase, pirellula glutamicum polypeptide small chain marina DSM coding #10001. [Blastopirellula 3645 sequence marina DSM fragment 3645] SEQ ID gi|87287337|gb|E NO: 1935. AQ79237.1| D-amino acid dehydrogenase, small chain [Blastopirellula marina DSM 3645] 505, D-amino acid 87309573 1.00E−127 Blasto- P. ADQ03060 2.00E−43 Propioni- ACF64502 0.93 1.4.99.1 1266 421 0 416 50 506 dehydrogenase, pirellula aeruginosa bacterium small chain marina DSM virulence acnes [Blastopirellula 3645 gene VIR14, predicted marina DSM protein. ORF-encoded 3645] polypeptide gi|87287337|gb|E #300. AQ79237.1| D-amino acid dehydrogenase, small chain [Blastopirellula marina DSM 3645] 507, D-amino-acid 92118208 1.00E−174 Nitrobacter Glyphosate AAR22262 6.00E−55 M. ACL63896 0.059 1.4.99.1 1254 417 0 433 71 508 dehydrogenase hamburgensis oxidoreductase xanthus [Nitrobacter X14 gene protein hamburgensis downstream sequence, X14] flanking seq id 9726. region. 509, D-amino-acid 86750758 1.00E−115 Rhodo- Glyphosate AAR22262 3.00E−53 Maize ADP48960 3.6 1.4.99.1 1245 414 0 417 49 510 dehydrogenase pseudomonas oxidoreductase carotenoid [Rhodo- palustris gene cleavage pseudomonas HaA2 downstream dioxygenase palustris flanking (CCD1) HaA2] region. protein SEQ ID NO: 4. 511, D-amino-acid 73541345 1.00E−166 Ralstonia Glyphosate AAR22262 1.00E−42 Klebsiella ACH97397 0.015 1.4.99.1 1266 421 0 414 65 512 dehydrogenase eutropha oxidoreductase pneumoniae [Ralstonia JMP134 gene polypeptide eutropha downstream seqid 7178. JMP134] flanking region. 513 D-amino acid 134093986 0 Herminii- P. ADQ03060 4.00E−94 Prokaryotic ACA35076 2.00E−04 1.4.99.1 1269 422 0 443 77 dehydrogenase monas aeruginosa essential gene small subunit arsenico- virulence #34740. [Herminiimonas xydans gene VIR14, arsenicoxydans] protein. gi|133737889|em b|CAL60934.1| D-amino acid dehydrogenase small subunit [Herminiimonas arsenicoxydans] 515, D-amino acid 146284421 1.00E−177 Pseudomonas Acinetobacter ADA36279 1.00E−124 Alpha1- AEF51726 0.91 1.4.99.1 1242 413 0 419 74 516 dehydrogenase; stutzeri baumannii antitrypsin small subunit A1501 protein specific [Pseudomonas #19. probe, stutzeri A1501] AATpro. 517, D-amino acid 126646463 1.00E−110 Algoriphagus Acinetobacter ADA33588 5.00E−46 Human ACA63029 0.058 1.4.99.1 1239 412 0 415 48 518 dehydrogenase sp. PR1 baumannii DICE-1-like [Algoriphagus protein RNA sp. PR1] #19. helicase. gi|126578095|gb| EA282315.1| D-amino acid dehydrogenase [Algoriphagus sp. PR1] 519, D-amino acid 88712395 3.00E−98 Flavo- N. ABP80542 4.00E−45 PCR primer AAX91990 0.015 1.4.99.1 1251 416 0 416 43 520 dehydrogenase bacteriales gonorrhoeae used to [Flavobacteriales bacterium nucleotide amplify an bacterium HTCC2170 sequence SEQ ORF of HTCC2170] ID 4691. Chlamydia gi|88708933|gb|E pneumoniae. AR01167.1] D-amino acid dehydrogenase [Flavo- bacteriales bacterium HTCC2170] 521, D-amino-acid 111019145 0 Rhodococcus C AAG93079 1.00E−102 Ryegrass ABK13581 0.015 1.4.99.1 1254 417 0 415 79 522 dehydrogenase sp. RHA1 glutamicum caffeic acid small subunit coding O-methyl [Rhodococcus sequence transferase sp. RHA1] fragment (OMT) SEQ ID genomic NO: 1935. sequence #2. 523, D-amino acid 126646463 7.00E−87 Algoriphagus H. pylori AAW98270 7.00E−48 Human ACN44966 0.23 1.4.99.1 1245 414 0 415 40 524 dehydrogenase sp. PR1 GHPO protein [Algoriphagus 1099 gene. sequence sp. PR1] hCP39072. gi|126578095|gb| EAZ82315.1] D-amino acid dehydrogenase [Algoriphagus sp. PR1] 525, D-amino-acid 121530396 1.00E−146 Ralstonia Glyphosate AAR22262 2.00E−45 PCR primer AAA58472 0.91 1. . . 1242 413 0 416 61 526 dehydrogenase pickettii 12J oxidoreductase used to [Ralstonia gene amplify pickettii 12J] downstream bleomycin gi|121302471 |gb| flanking (BLM) gene EAX43440.1| region. cluster ORF15. D-amino-acid dehydrogenase [Ralstonia pickettii 12J] 527, putative 116250556 0 Rhizobium Glyphosate AAR22262 4.00E−47 Bacterial ADS59863 0.058 1 . . . 1248 415 0 415 78 528 d-amino acid legumino- oxidoreductase polypeptide dehydrogenase sarum gene #10001. small subunit bv. viciae downstream [Rhizobium 3841 flanking leguminosarum region. bv. viciae 3841] 529, D-amino-acid 92118208 1.00E−116 Nitrobacter Glyphosate AAR22262 7.00E−48 RT-PCR ADZ14743 0.058 1.4.99.1 1245 414 0 433 53 530 dehydrogenase hamburgensis oxidoreductase primer to [Nitrobacter X14 gene amplify human hamburgensis downstream tumor X14] flanking associated region. antigen RNA Seq 28. 531, probable 149824671 1.00E−150 Limnobacter P. ADQ03060 1.00E−93 Pseudomonas ABD17880 0.001 1.4.99.1 1257 418 0 429 59 532 D-amino acid sp. MED105 aeruginosa aeruginosa dehydrogenase virulence polypeptide subunit gene VIR14, #3. [Limnobacter protein. sp. MED105] 533, D-amino acid 88712395 3.00E−88 Flavo- M. ADL05210 5.00E−46 Prokaryotic ACA35414 0.93 1.4.99.1 1269 422 0 416 40 534 dehydrogenase bacteriales catarrhalis essential gene [Flavobacteriales bacterium protein #1. #34740. bacterium HTCC2170 HTCC2170] gi|88708933|gb|E AR01167.1] D-amino acid dehydrogenase [Flavobacteriales bacterium HTCC2170] 535, Aldehyde 73537548 1.00E−143 Ralstonia Bacterial ADN25785 1.00E−140 Bacterial ADS55665 1.00E−12 1.2.1. 1581 526 0 525 52 536 dehydrogenase eutropha polypeptide polypeptide [Ralstonia JMP134 #10001. #10001. eutropha JMP134] 537, D-amino-acid 73541345 1.00E−136 Ralstonia Glyphosate AAR22262 1.00E−47 Streptomyces ABN88913 3.6 1.4.99.1 1260 419 0 414 57 538 dehydrogenase eutropha oxidoreductase sp. [Ralstonia JMP134 gene cytochrome eutropha downstream P450 JMP134] flanking related PCR region. primer SEQ ID NO: 18. 539, D-amino-acid 115359189 0 Burkholderia Glyphosate AAR22262 3.00E−47 Pseudomonas ABD17784 0.058 1.4.99.1 1242 413 0 413 96 540 dehydrogenase cepacia oxidoreductase aeruginosa [Burkholderia AMMD gene polypeptide cepacia downstream #3. AMMD] flanking region. 541, D-amino acid 21243478 1.00E−139 Xanthomonas P. ADQ03060 3.00E−40 M. ABQ90794 0.059 1.4.99.1 1266 421 1251 416 57 542 dehydrogenase axonopodis aeruginosa capsulatus subunit pv. citri str. virulence gene #766 [Xanthomonas 306 gene VIR14, for DNA axonopodis pv. protein. array. citri str. 306]. 543, D-amino acid 124009931 1.00E−107 Microscilla H. pylori AAW98270 3.00E−50 lactic acid ADF77343 3.6 1.4.99.1 1248 415 0 427 45 544 dehydrogenase marina ATCC GHPO bacteria small subunit, 23134 1099 gene. lactobacillus putative johnsonii [Microscilla La1 marina ATCC genomic 23134] DNA gi|123984082|gb| SEQ ID EAY24455.1| NO: 1. D-amino acid dehydrogenase small subunit, putative [Microscilla marina ATCC 23134] 545, D-amino-acid 86750758 1.00E−117 Rhodo- Glyphosate AAR22262 7.00E−51 Arabidopsis AAC41739 0.24 1.4.99.1 1296 431 0 417 50 546 dehydrogenase pseudomonas oxidoreductase thaliana [Rhodo- palustris gene protein pseudomonas HaA2 downstream fragment palustris flanking SEQ ID HaA2] region. NO: 76191. 547, D-amino acid 88712395 3.00E−86 Flavo- Photor- ABM69115 5.00E−53 Bacterial ADS50144 0.93 1.4.99.1 1266 421 0 416 39 548 dehydrogenase bacteriales habdus polypeptide [Flavobacteriales bacterium luminescens #10001. bacterium HTCC2170 protein HTCC2170] sequence gi|88708933|gb|E #59. AR01167.1] D-amino acid dehydrogenase [Flavobacteriales bacterium HTCC2170] 549, probable 149824671 1.00E−146 Limnobacter P. ADQ03060 9.00E−96 Human AAL13181 2.00E−04 1.4.99.1 1254 417 0 429 59 550 D-aminoacid sp. MED105 aeruginosa breast dehydrogenase virulence cancer subunit gene VIR14, expressed [Limnobacter protein. polynucleotide sp. MED105] 8440. 551, delta-1-pyrroline- 68535524 0 Coryne- Coryne- AAB79787 0 Propioni- ACF64460 0.003 1.5.1.12 3306 1101 0 1158 49 552 5-carboxylate bacterium bacterium bacterium dehydrogenase jeikeium glutamicum acnes [Coryne- K411 MP predicted bacterium protein ORF-encoded jeikeium K411] sequence polypeptide SEQ ID #300. NO: 1148. 553, D-amino-acid 115523700 1.00E−112 Rhodo- Glyphosate AAR22262 1.00E−53 Plan full ADO81304 0.92 1.4.99.1 1254 417 0 417 49 554 dehydrogenase pseudomonas oxidoreductase length [Rhodo- palustris gene insert pseudomonas BisA53 downstream polynucleotide palustris flanking seqid 4980. BisA53] region. 555, D-amino-acid 92118208 1.00E−118 Nitrobacter Glyphosate AAR22262 2.00E−49 RT-PCR ADZ14743 0.058 1.4.99.1 1242 413 0 433 53 556 dehydrogenase hamburgensis oxidoreductase primer to [Nitrobacter X14 gene amplify hamburgensis downstream human tumor X14] flanking associated region. antigen RNA Seq 28. 557, D-amino-acid 113871743 1.00E−132 Sinorhizo- Glyphosate AAR22262 5.00E−41 Human ACN44650 0.23 1. . . 1260 419 0 417 55 558 dehydrogenase bium oxidoreductase protein [Sinorhizobium medicae gene sequence medicae WSM419 downstream hCP39072. WSM419] flanking gi|113726415|gb| region. EAU07507.1| D-amino-acid dehydrogenase [Sinorhizobium medicae WSM419] 559, D-amino-acid 113871743 1.00E−132 Sinorhizo- Glyphosate AAR22262 5.00E−41 Human ACN44650 0.23 1. . . 1260 419 0 417 55 560 dehydrogenase bium oxidoreductase protein [Sinorhizobium medicae gene sequence medicae WSM419 downstream hCP39072. WSM419] flanking gi|113726415|gb| region. EAU07507.1| D-amino-acid dehydrogenase [Sinorhizobium medicae WSM419] 561, NADH 21244546 1.00E−143 Xanthomonas M. ABM91806 1.00E−115 M. ACL64800 2.00E−05 1.6.99.3 1230 409 1293 430 62 69 562 dehydrogenase axonopodis xanthus xanthus [Xanthomonas pv. protein protein axonopodis pv. citri str. 306 sequence, sequence, citri str. 306]. seq id seq id 9726. 9726. 563, short chain 73541108 3.00E−66 Ralstonia M. ABM96904 1.00E−53 Cenar- AAA55186 5.00E−04 1.1.1.100 720 239 0 270 56 564 dehydrogenase eutropha xanthus chaeum [Ralstonia JMP134 protein symbiosum eutropha sequence, open JMP134] seq id reading 9726. frame protein sequence SEQ ID NO: 80. 565, D-amino-acid 115523700 1.00E−112 Rhodo- Glyphosate AAR22262 2.00E−54 Plan full ADO81304 0.92 1.4.99.1 1254 417 0 417 49 566 dehydrogenase pseudomonas oxidoreductase length [Rhodo- palustris gene insert pseudomonas BisA53 downstream polynucleotide palustris flanking seqid 4980. BisA53] region. 567, D-amino-acid 120608839 0 Acidovorax P. ADQ03060 2.00E−91 Klebsiella ACH97385 0.016 1.4.99.1 1344 447 0 444 69 568 dehydrogenase avenae subsp. aeruginosa pneumoniae [Acidovorax citrulli virulence polypeptide avenae subsp. AAC00-1 gene VIR14, seqid 7178. citrulli AAC00-1] protein. gi|120587303|gb| ABM30743.1| D-amino-acid dehydrogenase [Acidovorax avenae subsp. citrulli AAC00-1] 569, D-amino-acid 114570652 8.00E−91 Maricaulis P. ADQ03060 9.00E−80 Lung ABX92073 0.92 1.4.99.1 1260 419 0 427 42 570 dehydrogenase maris aeruginosa specific [Maricaulis MCS10 virulence protein (LSP) maris MCS10] gene VIR14, #21. gi|114341114|gb| protein. ABI66394.1| D-amino-acid dehydrogenase [Maricaulis maris MCS10] 571, AGR_L_3050p 15891640 1.00E−180 Agro- P. ADQ03060 1.00E−141 Klebsiella ABD01189 3.00E−13 1.4.99.1 1248 415 1257 418 76 74 572 [Agrobacterium bacterium aeruginosa pneumoniae tumefaciens]. tumefaciens virulence polypeptide gene VIR14, seqid 7178. protein. 573, D-amino acid 88806240 5.00E−85 Robiginitalea Enterobacter AEH60497 2.00E−47 Prokaryotic ACA26568 0.059 1.4.99.1 1263 420 0 417 39 574 dehydrogenase biformala cloacae essential gene [Robiginitalea HTCC2501 protein #34740. biformala amino acid HTCC2501] sequence - gi|88783849|gb|E SEQ ID 5666. AR15020.1| D-amino acid dehydrogenase [Robiginitalea biformata HTCC2501] 575, Aldehyde 72384235 1.00E−141 Ralstonia Bacterial ADN22241 1.00E−133 Bacterial ADS56451 2.00E−08 1.2.1 1584 527 0 530 53 576 dehydrogenase eutropha polypeptide polypeptide [Ralstonia JMP134 #10001. #10001. eutropha JMP134] 577, putative 116250556 1.00E−166 Rhizobium Glyphosate AAR22262 8.00E−44 M. ACL64632 0.91 1. . . 1242 413 0 415 67 578 d-amino acid legumino- oxidoreductase xanthus dehydrogenase sarum gene protein small subunit bv. viciae downstream sequence, [Rhizobium 3841 flanking seq id leguminosarum region. 9726. bv. viciae 3841] 579, D-amino-acid 86361166 0 Rhizobium P. ADQ03060 1.00E−143 Rhizobium AAV30459 7.00E−51 1.4.99.1 1254 417 0 422 83 580 dehydrogenase; etli aeruginosa species small subunit CFN 42 virulence symbolic protein gene VIR14, plasmid [Rhizobium etli protein. pNGR234. CFN 42] 581, D-amino-acid 73541345 1.00E−136 Ralstonia Glyphosate AAR22262 4.00E−47 Thalecress AEG64174 0.92 1.4.99.1 1260 419 0 414 57 582 dehydrogenase autropha oxidoreductase stress [Ralstonia JMP134 gene related autropha downstream protein SEQ JMP134] flanking ID NO: 80. region. 583, D-amino-acid 91779297 0 Burkholderia Glyphosate AAR22262 9.00E−48 Pseudomonas ABD13052 3.6 1.4.99.1 1233 410 0 410 83 584 dehydrogenase xenovorans oxidoreductase aeruginosa [Burkholderia LB400 gene polypeptide xenovorans downstream #3. LB400] flanking region. 585, D-amino-acid 121603011 0 Polaromonas Photor- ABM69115 3.00E−85 Klebsiella ABD01189 3.00E−04 1.4.99.1 1338 445 0 455 81 586 dehydrogenase naphthalen- habdus pneumoniae [Polaromonas ivorans CJ2 luminescens polypeptide naphthalen- protein seqid 7178. ivorans CJ2] sequence #59 587, D-amino acid 104781752 1.00E−139 Pseudomonas Glyphosate AAR22262 3.00E−50 Drosophila ABL10910 0.24 1.4.99.1 1290 429 0 414 57 588 dehydrogenase; entomophila oxidoreductase melanogaster small subunit L48 gene polypeptide family protein downstream SEQ ID [Pseudomonas flanking NO 24465. entomophila region. L48] 589, D-amino-acid 111019145 1.00E−166 Rhodococcus C AAG93079 1.00E−103 M. ACL64760 0.92 1.4.99.1 1254 417 0 415 78 590 dehydrogenase sp. RHA1 glutamicum xanthus small subunit coding protein [Rhodococcus sequence sequence, sp. RHA1] fragment seq id 9726. SEQ ID NO: 1935. 591, D-amino-acid 113871743 1.00E−145 Sinorhizo- Glyphosate AAR22262 9.00E−50 Plan full ADX35599 0.015 1. . . 1245 414 0 417 59 592 dehydrogenase bium oxidoreductase length [Sinorhizobium medicae gene insert medicae WSM419 downstream polynucleotide WSM419] flanking seqid 4980. gi|113726415|gb| region. EAU07507.1] D-amino-acid dehydrogenase [Sinorhizobium medicae WSM419] 593, D-amino-acid 86750758 1.00E−118 Rhodo- Glyphosate AAR22262 4.00E−56 Prokaryotic ACA26173 0.015 1.4.99.1 1272 423 0 417 50 594 dehydrogenase pseudomonas oxidoreductase essential gene [Rhodo- palustris gene #34740. pseudomonas HaA2 downstream palustris flanking HaA2] region. 595, D-amino-acid 92118208 0 Nitrobacter Glyphosate AAR22262 5.00E−56 Bacterial ADS55748 0.015 1.4.99.1 1257 418 0 433 73 596 dehydrogenase hamburgensis oxidoreductase polypeptide [Nitrobacter X14 gene #10001. hamburgensis downstream X14] flanking region. 597, D-amino acid 34497369 0 Chromo- P. ADQ03060 1.00E−166 Enterobacter AEH53102 3.00E−19 1.4.99.1 1317 438 0 435 71 598 dehydrogenase bacterium aeruginosa cloacae small subunit violaceum virulence protein [Chromo- ATCC gene VIR14, amino acid bacterium 12472 protein. sequence - violaceum SEQ ID 5666. ATCC 12472] 599, D-amino-acid 86361166 0 Rhizobium P. ADQ03060 1.00E−145 Rhizobium AAV30459 1.00E−61 1.4.99.1 1251 416 0 422 84 600 dehydrogenase; etli aeruginosa species small subunit CFN 42 virulence symbiotic protein gene VIR14, plasmid [Rhizobium etli protein. pNGR234. CFN 42] 601, D-amino-acid 86361166 0 Rhizobium P. ADQ03060 1.00E−145 Rhizobium AAV30459 2.00E−60 1.4.99.1 1254 417 0 422 81 602 dehydrogenase; etli CFN 42 aeruginosa species small subunit virulence symbiotic protein gene VIR14, plasmid [Rhizobium protein. pNGR234. etl iCFN 42] 603, D-amino acid 88712395 2.00E−93 Flavo- M. ADL05210 2.00E−54 Corn ear- ABX86147 0.23 1.4.99.1 1254 417 0 416 42 604 dehydrogenase bacteriales catarrhalis derived [Flavobacteriales bacterium protein #1. polynucleotide bacterium HTCC2170 (cpd) HTCC2170] #5394. gi|88708933|gb|E AR01167.1| D-amino acid dehydrogenase [Flavobacteriales bacterium HTCC2170] 605, D-amino-acid 113871743 1.00E−170 Sinorhizo- Glyphosate AAR22262 2.00E−48 Bacterial ADS56578 0.92 1. . . 1254 417 0 417 67 606 dehydrogenase bium oxidoreductase polypeptide [Sinorhizobium medicae gene #10001. medicae WSM419 downstream WSM419] flanking gi|113726415|gb| region. EAU07507.1] D-amino-acid dehydrogenase [Sinorhizobium medicae WSM419] 607, probable 149824671 1.00E−125 Limnobacter P. ADQ03060 1.00E−94 Insulin ADZ49334 0.91 1.4.99.1 1251 416 0 429 51 608 D-amino acid sp. MED105 aeruginosa signaling dehydrogenase virulence pathway subunit gene VIR14, related [Limnobacter protein. PCR primer, sp. MED105] SEQ ID 22. 609, D-amino-acid 121530396 1.00E−146 Ralstonia Glyphosate AAR22262 1.00E−45 Plant ADT18820 0.004 1. . . 1233 410 0 416 61 610 dehydrogenase pickettii 12J oxidoreductase polypeptide, [Ralstonia gene SEQ ID 5546. pickettii 12J] downstream gi|121302471|gb| flanking EAX43440.1| region. D-amino-acid dehydrogenase [Ralstonia pickettii 12J] 611, D-amino-acid 86361166 0 Rhizobium P. ADQ03060 1.00E−143 Rhizobium AAV30459 7.00E−51 1.4.99.1 1254 417 0 422 83 612 dehydrogenase; etli aeruginosa species small subunit CFN 42 virulence symbiotic protein gene VIR14, plasmid [Rhizobium etli protein. pNGR234. CFN 42] 613, D-amino acid 149377918 3.00E−98 Marinobacter N. ABP80542 3.00E−77 Arabidopsis ABQ66079 0.24 1.4.99.1 1287 428 0 423 43 614 dehydrogenase algicola gonorrhoeae thaliana small subunit DG893 nucleotide protein [Marinobacter sequence SEQ fragment algicola ID 4691. SEQ ID DG893] NO: 76191. 615, putative 116250556 0 Rhizobium Glyphosate AAR22262 3.00E−47 M. ACL64301 0.91 1. . . 1248 415 0 415 79 616 d-amino acid legumino- oxidoreductase xanthus dehydrogenase sarum gene protein small subunit bv. viciae downstream sequence, [Rhizobium 3841 flanking seq id 9726. leguminosarum region. bv. viciae 3841] 617, D-amino-acid 86750758 1.00E−113 Rhodo- Glyphosate AAR22262 1.00E−49 Plan full ADX64511 0.015 1.4.99.1 1245 414 0 417 49 618 dehydrogenase pseudomonas oxidoreductase length [Rhodo- palustris gene insert pseudomonas HaA2 downstream polynucleotide palustris flanking seqid 4980. HaA2] region. 619, aldehyde 118027543 1.00E−144 Burkholderia Bacterial ADN25785 1.00E−131 Bacterial ADS55665 1.00E−12 1.2.1.39 1593 530 0 526 51 620 dehydrogenase phymatum polypeptide polypeptide [Burkholderia STM815 #10001. #10001. phymatum STM815] gi|117986837|gb| EAV01212.1| aldehyde dehydrogenase [Burkholderia phymatum STM815] 621, D-amino acid 88712395 1.00E−92 Flavo- Pseudomonas ABO75104 1.00E−48 Bifido- ABQ81842 0.059 1.4.99.1 1266 421 0 416 42 622 dehydrogenase bacteriales aeruginosa bacterium [Flavobacteriales bacterium polypeptide longum bacterium HTCC2170 #3. NCC2705 HTCC2170] ORF amino gi|88708933|gb|E acid AR01167.1| sequence D-amino acid SEQ ID dehydrogenase NO: 408. [Flavobacteriales bacterium HTCC2170] 623, D-amino acid 77465126 1.00E−121 Rhodobacter Photorhabdus ABM69115 1.00E−111 Pseudomonas ABD08815 3.00E−07 1.4.99.1 1260 419 0 436 55 624 dehydrogenase sphaeroides luminescens aeruginosa small subunit 2.4.1 protein polypeptide [Rhodobacter sequence #59 #3. sphaeroides 2.4.1] 625, D-amino-acid 148553731 0 Sphingomonas P. ADQ03060 1.00E−143 P. ADQ03059 4.00E−09 1.4.99.1 1254 417 0 416 79 626 dehydrogenase wittichii aeruginosa aeruginosa [Sphingomonas RW1 virulence virulence wittichii RW1] gene VIR14, gene VIR14, protein. protein. 627, probable 149824671 1.00E−151 Limnobacter P. ADQ03060 2.00E−93 P. ADQ03059 0.059 1257 418 0 429 60 628 D-amino acid sp. aeruginosa aeruginosa dehydrogenase MED105 virulence virulence subunit gene VIR14, gene VIR14, [Limnobacter protein. protein. sp. MED105] 629, putative 39725441 5.00E−63 Streptomyces Streptomyces ADQ74690 1.00E−63 Streptomyces ADQ74672 0.17 1. . . 921 306 74787 305 630 dehydrogenase parvulus parvulus parvulus [Streptomyces borrelidin borrelidin parvulus] polyketide polyketide synthase synthase orfB8 orfB8 protein. protein. 631, FAD dependent 118037424 0 Burkholderia Glyphosate AAR22262 3.00E−49 Rice ACL30152 0.015 1.4.99.1 1233 410 0 465 86 632 oxidoreductase phytofirmans oxidoreductase abiotic [Burkholderia PsJN] gene stress phytofirmans downstream responsive PsJN] flanking polypeptide gi|117992233|gb| region. SEQ ID EAV06525.1| NO: 4152. FAD dependent oxidoreductase [Burkholderia phytofirmans PsJN] 633, FAD dependent 118734342 0 Delftia Acinetobacter ADA33588 1.00E−174 Pseudomonas ABD08815 8.00E−23 1.4.99.1 1293 430 0 432 74 634 oxidoreductase acidovorans baumannii aeruginosa [Delftia SPH-1 protein polypeptide acidovorans #19. #3. SPH-1] gi|118665742|gb| EAV72348.1| FAD dependent oxidoreductase [Delftia acidovorans SPH-1] 635, FAD dependent 118734342 0 Delftia Acinetobacter ADA33588 1.00E−175 Pseudomonas ABD08815 2.00E−32 1.4.99.1 1296 431 0 432 76 636 oxidoreductase acidovorans baumannii aeruginosa [Delftia SPH-1 protein polypeptide acidovorans #19. #3. SPH-1] gi|118665742|gb| EAV72348.1| FAD dependent oxidoreductase [Delftia acidovorans SPH-1] 77457196 0 Pseudomonas Pseudomonas ABO71517 1.00E−151 Pseudomonas ABD05088 2.00E−66 1.1.99. 1128 375 0 375 87 637, FAD dependent fluorescens aeruginosa aeruginosa 638 oxidoreductase PfO-1 polypeptide polypeptide [Pseudomonas #3. #3. fluorescens PfO-1] 639, oxidoreductase- 118061388 4.00E−72 Roseiflexus Hyper- ADM25642 2.00E−38 Prokaryotic ACA27410 0.18 1.1.1. 1002 333 0 345 44 640 like castenholzii thermophile essential gene [Roseiflexus DSM 13941 Methano- #34740. castenholzii pyrus DSM 13941] kandleri gi|118014488|gb| protein #28. EAV28464.1| oxidoreductase- like [Roseiflexus castenholzii DSM 13941] 641, FAD dependent 77457196 0 Pseudomonas Pseudomonas ABO71517 1.00E−151 Pseudomonas ADB05088 2.00E−66 1.1.99.1 1128 375 0 375 87 642 oxidoreductase fluorescens aeruginosa aeruginosa [Pseudomonas PfO-1 polypeptide polypeptide fluorescens #3. #3. PfO-1] 643, Glycine/D-amino 83311898 4.00E−86 Magneto- Pseudomonas ABO75104 8.00E−78 Human ABN17150 0.059 1.4.99.1 1266 421 0 422 42 644 acid oxidase spirillum aeruginosa ORFX [Magneto- magneticum polypeptide protein spirillum AMB-1 #3. sequence magneticum SEQ ID AMB-1] NO: 19716. 645, FAD dependent 92113847 1.00E−151 Chromo- Glyphosate AAR22262 2.00E−40 Silibacter AEM45684 0.23 1. . . 1245 414 0 414 62 646 oxidoreductase halobacter oxidoreductase sp. TM1040 [Chromo- salexigens gene gene SEQ halobacter DSM 3043 downstream ID NO: 3. salexigens DSM flanking 3043] region. 647, FAD dependent 118029195 1.00E−165 Burkholderia Enterobacter AEH60497 1.00E−150 Pseudomonas ABD08815 2.00E−06 1.4.99.1 1296 431 0 428 65 648 oxidoreductase phymatum cloacae aeruginosa [Burkholderia STM815 protein polypeptide phymatum amino acid #3. STM815] sequence - gi|117985258|gb| SEQ ID 5666. EAU99635.1| FAD dependent oxidoreductase [Burkholderia phymatum STM815] 649, FAD dependent 92113847 1.00E−146 Chromo- Glyphosate AAR22262 4.00E−43 Pseudomonas ABD05284 0.058 1.4.99.1 1245 414 0 414 61 650 oxidoreductase halobacter oxidoreductase aeruginosa [Chromo- salexigens gene polypeptide halobacter DSM 3043 downstream #3. salexigens DSM flanking 3043] region. 651, putative 27379412 1.00E−122 Bradyrhizo- Glyphosate AAR22262 1.00E−42 Prokaryotic ACA27392 0.058 1.4.99.1 1236 411 0 410 53 652 oxidoreductase bium oxidoreductase essential gene protein japonicum gene #34740. [Bradyrhizobium USDA 110 downstream japonicum flanking USDA 110] region. 653, reductase 66767863 1.00E−128 Xanthomonas Prokaryotic ABU14721 1.00E−119 Prokaryotic ACA36215 1.00E−15 1.8.1.7 1344 447 0 456 55 654 [Xanthomonas campestris essential gene essential gene campestris pv. pv. #34740. #34740. campestris str. campestris 8004] str. 8004 655, FAD dependent 75676467 1.00E−118 Nitrobacter Glyphosate AAR22262 5.00E−53 G sorghi ACF04822 0.94 1.4.99.1 1284 427 0 417 48 656 oxidoreductase winogradskyi oxidoreductase nitrilase [Nitrobacter Nb-255 gene protein winogradskyi downstream fragment #2. Nb-255] flanking region. 657, D-aspartate 115376852 3.00E−85 Stigmatella Human AED18771 6.00E−59 M. AAD55815 2.9 1.4.3.3 1008 335 0 314 45 658 oxidase aurantiaca D-aspartate carbonacea [Stigmatella DW4/3-1 oxidase polyketide aurantiaca active site. synthase DW4/3-1] (PKS) gi|115366155|gb| domain EAU65167.1| peptide #3. D-aspartate oxidase [Stigmatella aurantiaca DW4/3-1] 659, FAD dependent 71909453 1.00E−142 Dechloro- P. ADQ03060 6.00E−97 Human AAL13181 0.001 1.4.99.1 1245 414 0 418 62 660 oxidoreductase monas aeruginosa brest cancer [Dechloromonas aromatica virulence expressed aromatica RCB] RCB gene VIR14, polynucleotide protein. 8440. 661, putative secreted 78049397 1.00E−102 Xanthomonas Human AED18771 4.00E−07 Plant ADJ40271 0.056 1188 395 0 404 50 662 protein campestris D-aspartate cDNA #31. [Xanthomonas pv. oxidase campestris pv. vesicatoria active site. vesicatoria str. str. 85-10] 85-10 663, FAD dependent 75676467 1.00E−120 Nitrobacter Glyphosate AAR22262 6.00E−52 Plant full ADX45957 0.058 1.4.99.1 1245 414 0 417 52 664 oxidoreductase winogradskyi oxidoreductase length insert [Nitrobacter Nb-255 gene polynucleotide winogradskyi downstream seqid 4980. Nb-255] flanking region. 665, Glycine/D-amino 88810939 5.00E−83 Nitrococcus M. ADL05210 6.00E−71 Aspergillus ABT17713 0.92 1.4.99.1 1263 420 0 423 41 666 acid oxidase mobilis catarrhalis fumigatus [Nitrococcus Nb-231 protein #1. essential mobilis Nb-231] gene gi|88791478|gb|E protein #821. AR22589.1| Glycine/D-amino acid oxidase [Nitrococcus mobilis Nb-231] 667, FAD dependent 116624522 5.00E−82 Solibacter Human AED18771 5.00E−17 DNA AAS83512 0.81 1.4.3.1 1113 370 0 377 45 668 oxidoreductase usitatus D-aspartate encoding [Solibacter Ellin6076 oxidase nove human usitatus active site. diagnostic Ellin6076] protein gi|116227684|gb| #20574. ABJ86393.1| FAD dependent oxidoreductase [Solibacter usitatus Ellin6076] 669, FAD dependent 75676467 1.00E−122 Nitrobacter Glyphosate AAR22262 2.00E−46 A. AAV21187 0.92 1.4.99.1 1260 419 0 417 52 670 oxidoreductase winogradskyi oxidoreductase mediterranei- [Nitrobacter Nb-255 gene rifamycin winogradskyi downstream synthesis Nb-255] flanking gene cluster region. fragment protein F. 671, FAD dependent 118038076 0 Burkholderia Glyphosate AAR22262 6.00E−46 Oligo- ABQ19293 3.6 1.4.99.1 1242 413 0 413 82 672 oxidoreductase phytofirmans oxidoreductase nucleotide for [Burkholderia PsJN gene detecting phytofirmans downstream cytosine PsJN] flanking methylation gi|117991720|gb| region. SEQ ID EAV06013.1| NO 20311. FAD dependent oxidoreductase [Burkholderia phytofirmans PsJN] 673, FAD dependent 118038076 0 Burkholderia Glyphosate AAR22262 1.00E−45 Oligo- ABQ19293 3.6 1.4.99.1 1242 413 0 413 82 674 oxidoreductase phytofirmans oxidoreductase nucleotide for [Burkholderia PsJN gene detecting phytofirmans downstream cytosine PsJN] flanking methylation gi|117991720|gb| region. SEQ ID EAV06013.1| FAD NO 20311. dependent oxidoreductase [Burkholderia phytofirmans PsJN] 675, FAD dependent 118038076 0 Burkholderia Glyphosate AAR22262 1.00E−45 Oligo- ABQ19293 3.6 1.4.99.1 1242 413 0 413 82 676 oxidoreductase phytofirmans oxidoreductase nucleotide for [Burkholderia PsJN gene detecting phytofirmans downstream cytosine PsJN] flanking methylation gi|117991720|gb| region. SEQ ID EAV06013.1| NO 20311. FAD dependent oxidoreductase [Burkholderia phytofirmans PsJN] 677, D-amino-acid 119716015 2.00E−51 Nocardioides Human AED18771 4.00E−36 Prokaryotic ACA45556 3.6 1.4.3.3 909 302 0 310 42 678 oxidase sp. JS614 D-aspartate essential gene [Nocardioides oxidase #34740. sp. JS614] active site. gi|119536676|gb| ABL81293.11 D-amino-acid oxidase [Nocardioides sp. JS614] 679, FAD dependent 118731339 0 Delftia Pseudomonas ABO71517 1.00E−102 Pseudomonas ABD04986 4.00E−09 1.1.99. 1161 386 0 385 83 680 oxidoreductase acidovorans aeruginosa aeruginosa [Delftia SPH-1 polypeptide polypeptide acidovorans #3. #3. SPH-1] gi|118668198|gb| EAV74793.11 FAD dependent oxidoreductase [Delftia acidovorans SPH-1] 681, Glycine/D-amino 88810939 5.00E−94 Nitrococcus Pseudomonas ADQ03060 5.00E−85 N ADC76718 0.93 1.4.99.1 1266 421 0 423 43 682 acid oxidase mobilis aeruginosa benthamiana [Nitrococcus Nb-231 polypeptide phytopathogen mobilis Nb-231] #3. resistance- gi|88791478|gb|E related AR22589.1| contig Glycine/D-amin cDNA - acid oxidase SEQ ID 5. [Nitrococcus mobilis Nb-231] 683, FAD dependent 114319576 2.00E−86 Alkalilimni- Pseudomonas ABO75104 2.00E−77 C. AAF71130 0.059 1.4.99.1 1251 416 0 421 42 684 oxidoreductase cola aeruginosa glutamicum [Alkalilimnicola ehrlichei polypeptide SRT protein ehrlichei MLHE-1 #3. sequence MLHE-1] SEQ ID NO: 264. 685, FAD dependent 114319576 5.00E−86 Alkalilimni- M. ADL05210 3.00E−63 Human ABT07579 0.06 1.4.99.1 1281 426 0 421 40 686 oxidoreductase cola catarrhalis breast cancer [Alkalilimnicola ehrlichei protein #1. associated ehrlichei MLHE-1 coding MLHE-1] sequence SEQ ID NO: 79. 687, D-aspartate 115376852 2.00E−71 Stigmatella Human AED18771 2.00E−51 Bacterial ADS56436 0.044 1.4.3.3 957 318 0 314 44 688 oxidase aurantiaca D-aspartate polypeptide [Stigmatella DW4/3-1 oxidase #10001. aurantiaca active site. DW4/3-1] gi|115366155|gb| EAU65167.1| D-aspartate oxidase [Stigmatella aurantiaca DW4/3-1] 699, FAD dependent 118716641 1.00E−154 Burkholderia Glyphosate AAR22262 5.00E−53 Plant full ADX50476 0.23 1.4.99.1 1248 415 0 548 64 690 oxidoreductase multivorans oxidoreductase length insert [Burkholderia ATCC gene poly- multivorans 17616 downstream nucleotide ATCC 17616] flanking seqid 4980. gi|118660081 |gb| region. EAV66824.1| FAD dependent oxidoreductase [Burkholderia multivorans ATCC 17616] 691, FAD dependent 94314499 1.00E−159 Ralstonia Acinetobacter ADA36279 1.00E−128 N. AAA81470 0.058 1.4.99.1 1239 412 0 422 66 692 oxidoreductase metallidurans baumannii meningitides [Ralstonia CH34 protein partial DNA metallidurans #19. sequence CH34] gnm_640 SEQ ID NO: 640. 693, NADH: 110634071 9.00E−58 Mesorhizo- Drosophila ABB59730 3.00E−09 Prokaryotic ACA34135 3.9 1.6.99.3 396 131 0 132 73 694 ubiquinone bium sp. melanogaster essential gene oxidoreductase BNC1 polypeptide #34740. 17.2 kD subunit SEQ ID [Mesorhizobium NO 24465. sp. BNC1] 695, FAD dependent 116695075 1.00E−141 Ralstonia Pseudomonas ABO71517 1.00E−121 Pseudomonas ABD05088 1.00E−12 1.1.99. 1164 387 0 388 63 696 oxidoreductase eutropha aeruginosa aeruginosa [Ralstonia H16 polypeptide polypeptide eutropha H16] #3. #3. 697, Glycine/D-amino 83311898 1.00E−103 Magnet- P. ADQ03060 2.00E−50 Bacterial ADT43170 0.001 1.4.99.1 1257 418 0 422 47 698 acid oxidase ospirillum aeruginosa polypeptide [Magnet- magneticum virulence #10001. ospirillum AMB-1 gene VIR14, magneticum protein. AMB-1] 699, FAD dependent 75676467 1.00E−119 Nitrobacter Glyphosate AAR22262 1.00E−41 Prokaryotic ACA26065 0.015 1.4.99.1 1245 414 0 417 52 700 oxidoreductase winogradskyi oxidoreductase essential gene [Nitrobacter Nb-255 gene #34740. winogradskyi downstream Nb-255] flanking region. 701, D-aspartate 115376852 8.00E−51 Stigmatella Human AED18771 6.00E−44 Drosophila ABL13112 0.18 1.4.3.3 981 326 0 314 39 702 oxidase aurantiaca D-aspartate melanogaster [Stigmatella DW4/3-1 oxidase polypeptide aurantiaca active site. SEQ ID DW4/3-1] NO 24465. gi|115366155|gb| EAU65167.1| D-aspartate oxidase [Stigmatella aurantiaca DW4/3-1] 703, FAD dependent 91976294 1.00E−118 Rhodo- Glyphosate AAR22262 6.00E−44 Hyper- ADM27081 0.23 1.4.99.1 1248 415 0 417 50 704 oxidoreductase pseudomo oxidoreductase thermophile [Rhodo- nas palustris gene Methanopyrus pseudomo BisB5 downstream kandleri nas palustris flanking protein #28. BisB5] region. 705, putative 27379412 1.00E−133 Bradyrhizo- Glyphosate AAR22262 1.00E−42 Pseudomonas ABD08105 0.23 1.4.99.1 1233 410 0 410 56 706 oxidoreductase bium oxidoreductase aeruginosa protein japonicum gene polypeptide [Bradyrhizobium USDA 110 downstream #3. japonicum flanking USDA 110] region. 707, FAD dependent 75676467 1.00E−121 Nitrobacter Glyphosate AAR22262 6.00E−52 S. ADY66589 0.06 1.4.99.1 1275 424 0 417 52 708 oxidoreductase winogradskyi oxidoreductase mansoni [Nitrobacter Nb-255 gene protein winogradskyi downstream SEQ ID 18. Nb-255] flanking region. 709, FAD dependent 118034565 0 Burkholderia Glyphosate AAR22262 2.00E−49 Bacterial ADT43021 0.23 1.4.99.1 1233 410 0 410 75 710 oxidoreductase phymatum oxidoreductase polypeptide [Burkholderia STM815 gene #10001. phymatum downstream STM815] flanking gi|117979745|gb| region. EAU94152.1| 711, FAD dependent 114319576 1.00E−88 Alkalilim- P. ADQ03060 2.00E−81 Rice protein ADA48957 0.23 1.4.99.1 1266 421 0 421 43 712 oxidoreductase nicola aeruginosa conferring [Burkholderia ehrlichei virulence disease phymatum MLHE-1 gene VIR14, resistance STM815] protein. in plants/ FAD dependent oxidoreductase [Alkalilimnicola ehrlichei MLHE-1] 713, FAD dependent 118034565 0 Burkholderia Glyphosate AAR22262 2.00E−49 Bacterial ADT43021 0.23 1.4.99.1 1233 410 0 410 75 714 oxidoreductase phymatum oxidoreductase polypeptide [Burkholderia STM815 gene #10001. phymatum downstream STM815] flanking gi|117979745|gb| region. EAU94152.1| 715, FAD dependent 144897812 4.00E−87 Magneto- Glyphosate ABO75104 4.00E−80 Human AET00128 0.91 1.4.99.1 1251 416 0 420 43 716 oxidoreductase spirillum oxidoreductase acute [Burkholderia gryphiswal- gene myeloid phymatum dense downstream leukemia STM815] MSR-1 flanking prognosis FAD dependent region. target gene oxidoreductase PCR primer [Magnetospirillum #247. gryphiswaldense MSR-1] 717, FAD dependent 124006998 1.00E−29 Microscilla H. AAW98270 0.87 0 912 303 0 358 29 718 oxidoreductase, marina ATCC pylori putative 23134 GHPO [Microscilla 1099 gene. marina ATCC 23134] gi|123987451|gb| EAY27171.1| 719, FAD dependent 15676467 1.00E−137 Nitrobacter Glyphosate AAR22262 6.00E−51 Pseudomonas ABD10326 3.6 1.4.99.1 1251 416 0 417 57 720 oxidoreductase, winogradskyi oxidoreductase aeruginosa putative Nb-255 gene polypeptide [Microscilla downstream #3. marina ATCC flanking 23134] region. FAD dependent oxidoreductase [Nitrobacter winogradskyi Nb-255] 721, D-amino-acid 119716015 1.00E−53 Nocardioides Human AED18771 6.00E−40 Bacterial ADT44787 0.042 1.4.3.3 918 305 0 310 42 722 oxidase sp. JS614 D-aspartate polypeptide [Nocardioides oxidase #10001. sp. JS614] active stie. gi|119536676|gb| ABL81293.1| D-amino-acid oxidase [Nocardioides sp. JS614] 723, Glycine/D-amino 88810939 2.00E−84 Nitrococcus Pseudomonas ABO75104 2.00E−77 Human breast AAL13181 0.001 1.4.99.1 1251 416 0 423 41 724 acid oxidase mobilis aeruginosa cancer [Nitrococcus Nb-231 polypeptide expressed mobilis Nb-231] #3. polynucleotide gi|88791478|gb|E 8440. AR22589.1] Glycine/D-amino acid oxidase [Nitrococcus mobilis Nb-231] 725, FAD dependent 71909453 1.00E−133 Dechloro- Enterobacter AEH60497 4.00E−95 Human ORFX ABN17150 1.00E−12 1.4.99.1 1260 419 0 418 56 726 oxidoreductase monas cloacae protein [Dechloromonas aromatica protein sequence aromatica RCB] RCB amino acid SEQ ID sequence - NO: 19716. SEQ ID 5666. 727, D-amino acid 149377918 1.00E−136 Marinobacter Photorhabdus ABM69115 7.00E−85 Drosophila ABL15242 3.9 1.4.99.1 1329 442 0 423 56 728 dehydrogenase algicola luminescens melanogaster small subunit DG893 protein polypeptide [Marinobacter sequence #59. SEQ ID algicola NO 24465. DG893] 729, pyruvate 114319858 1.00E−135 Alkalilim- Staphy- ABM73103 1.00E−57 Pseudomonas ABD01828 0.051 1.2.7.3 1098 365 0 576 65 730 flavodoxin/ nicola lococcus aeruginosa ferredoxin ehrlichei aureus polypeptide oxidoreductase MLHE-1 protein #10. #3. domain protein [Alkalilimnicola ehrlichei MLHE-1] 731, FAD dependent 144897812 4.00E−80 Magneto- Klebsiella ABO67618 4.00E−62 Human breast AAL13181 1.00E−06 1.4.99.1 1260 419 0 420 41 732 oxidoreductase spirillum pneumoniae cancer [Magnetospirillum gryphiswal- polypeptide expressed gryphiswaldense dense seqid 7178. polynucleotide MSR-1] MSR-1 8440. 733, FAD dependent 144897812 1.00E−89 Magneto- Pseudomonas ABO75104 8.00E−76 Bacterial ADS63429 0.23 1.4.99.1 1257 418 0 420 44 734 oxidoreductase spirillum aeruginosa polypeptide [Magnetospirillum gryphiswal- polypeptide #10001. gryphiswaldense dense #3. MSR-1] MSR-1 735, FAD linked 149124512 3.00E−61 Methylo- FAD- ADM97925 2.00E−57 M. ACL64584 2.00E−06 1.1.2.4 807 268 0 477 48 736 oxidase domain bacterium dependent- xanthus protein sp. 4-46 D-erythronate protein [Methylobacte- 4-phosphate sequence, rium sp. 4-46] dehydro- seq id 9726. genase. 737, FAD dependent 92113847 1.00E−150 Chromo- Glyphosate AAR20642 2.00E−43 Pseudomonas ABD10634 0.058 1.4.99.1 1242 413 0 414 62 738 oxidoreductase halobacter oxidoreductase aeruginosa [Chromohalo- salexigens gene polypeptide bacter salexigens DSM 3043 downstream #3. DSM 3043] flanking region. 739, FAD dependent 92113847 1.00E−153 Chromo- Glyphosate AAR22262 6.00E−41 Human AAD07816 0.91 1. . . 1245 414 0 414 63 740 oxidoreductase halobacter oxidoreductase secreted [Chromohalo- salexigens gene protein- bacter salexigens DSM 3043 downstream encoding gene DSM 3043] flanking 29 cDNA region. clone HCEFI77, SEQ ID NO: 103. 741, FAD dependent 92113847 1.00E−153 Chromo- Glyphosate AAR22262 6.00E−41 Human AAD07816 0.91 1. . . 1245 414 0 414 63 742 oxidoreductase halobacter oxidoreductase secreted [Chromohalo- salexigens gene protein- bacter salexigens DSM 3043 downstream encoding gene DSM 3043] flanking 29 cDNA region. clone HCEFI77, SEQ ID NO: 103. 743, FAD dependent 92113847 1.00E−150 Chromo- Glyphosate AAR20642 6.00E−43 Pseudomonas ABD10634 0.058 1.4.99.1 1242 413 0 414 62 744 oxidoreductase halobacter oxidoreductase aeruginosa [Chromohalo- salexigens gene polypeptide bacter salexigens DSM 3043 downstream #3. DSM 3043] flanking region. 745, FAD dependent 94314005 1.00E−101 Ralstonia Pseudomonas ABO71517 6.00E−85 E. coli AER28868 0.83 1.1.99. 1146 381 0 385 49 746 oxidoreductase metallidurans aeruginosa NCg12640 [Ralstonia CH34 polypeptide DNA. metallidurans #3. CH34] 747, glycine/D-amino 121525574 1.00E−125 Parvibaculum P. ADQ03060 6.00E−76 Pseudomonas ABD08815 0.015 1.4.99.1 1278 425 0 462 53 748 acid oxidase lavamenti- aeruginosa aeruginosa [Parvibaculum vorans virulence polypeptide lavamentivorans DS-1 gene VIR14, #3. DS-1] protein. gi|121298521|gb| EAX39710.1| glycine/D-amino acid oxidase [Parvibaculum lavamentivorans DS-1] 749, FAD dependent 71909453 1.00E−139 Dechloro- P. ADQ03060 2.00E−96 Human breast AAL13181 2.00E−07 1.4.99.1 1245 414 0 418 60 750 oxidoreductase monas aeruginosa cancer [Dechloromonas aromatica virulence expressed aromatica RCB] RCB gene VIR14, polynucleotide protein. 8440. 751, monooxygenase, 118050299 8.00E−81 Comamonas Farnesyl ADR01274 6.00E−18 Prokaryotic ACA23297 0.65 1.14.13. 915 304 0 543 49 752 FAD-binding testosteroni dibenzo- essential gene [Comamonas KF-1 diazepinone #34740. testosteroni KF-1] biosynthetic gi|118002385|gb| ORF9 protein EAV16539.1| HMGA, monooxygenase, SEQ ID 18. FAD-binding [Comamonas testosteroni KF-1] 753, FAD dependent 75676467 1.00E−120 Nitrobacter Glyphosate AAR22262 3.00E−53 Human ADD01267 0.23 1.4.99.1 1248 415 0 417 52 754 oxidoreductase winogradskyi oxidoreductase nucleic acid- [Nitrobacter Nb-255 gene associated winogradskyi downstream protein Nb-255] flanking NAAP-13 region. SEQ ID NO: 13. 755, FAD dependent 91976294 1.00E−121 Rhodo- Glyphosate AAR22262 1.00E−54 0 1.4.99.1 1245 414 0 417 51 756 oxidoreductase pseudomo oxidoreductase [Rhodo- nas palustris gene pseudomonas BisB5 downstream palustris flanking BisB5] region. 757, putative 126732124 1.00E−131 Sagittula Glyphosate AAR22262 1.00E−31 Human ADJ09909 3.6 1.4.99.1 1251 416 0 410 55 758 oxidoreductase stellate E-37 oxidoreductase prostate protein [Sagittula gene cancer stellate E-37] downstream associated gi|126707413|gb| flanking polypeptide EBA06477.1| region. SeqID273. putative oxidoreductase protein [Sagittula stellate E-37] 759, FAD dependent 75676467 1.00E−113 Nitrobacter Glyphosate AAR22262 2.00E−51 Human PRO AEH18256 0.23 1.4.99.1 1254 417 0 417 49 760 oxidoreductase winogradskyi oxidoreductase protein amino [Nitrobacter Nb-255 gene acid winogradskyi downstream sequence - Nb-255] flanking SEQ ID 59. region. 761, FAD dependent 92113847 1.00E−149 Chromohalo- Glyphosate AAR20642 2.00E−42 Nocardiopsis ADR47152 0.23 1.4.99.1 1242 413 0 414 62 762 oxidoreductase bacter oxidoreductase alba copper [Chromohalo- salexigens gene stable bacter salexigens DSM 3043 downstream protease 08. DSM 3043] flanking region. 763, FAD dependent 114319576 4.00E−84 Alkalilim- Pseudomonas ABO75104 9.00E−80 Human breast AAL13181 0.001 1.4.99.1 1251 416 0 421 41 764 oxidoreductase nicola aeruginosa cancer [Alkalilimnicola ehrlichei polypeptide expressed ehrlichei MLHE-1 #3. polynucleotide MLHE-1] 8440. 765, FAD linked 149124512 1.00E−106 Methylo- FAD- ADM97925 1.00E−94 Bacterial ADS59768 9.00E−07 1.1.2.4 1182 393 0 477 51 766 oxidase domain bacterium dependent-D- polypeptide protein sp. 4-46 erythronate 4- #10001. [Methylobacte- phosphate rium sp. 4-46] dehydrogenase. 767, FAD dependent 114319576 3.00E−84 Alkalilim- Pseudomonas ABO75104 2.00E−75 Human breast AAL13181 0.015 1.4.99.1 1257 418 0 421 41 768 oxidoreductase nicola aeruginosa cancer [Alkalilimnicola ehrlichei polypeptide expressed ehrlichei MLHE-1 #3. polynucleotide MLHE-1] 8440. 769, FAD dependent 92113847 1.00E−149 Chromohalo- Glyphosate AAR20642 2.00E−42 Prokaryotic ACA37735 0.015 1.4.99.1 1242 413 0 414 62 770 oxidoreductase bacter oxidoreductase essential gene [Chromohalo- salexigens gene #34740. bacter salexigens DSM 3043 downstream DSM 3043] flanking region. 771, FAD dependent 75676467 1.00E−121 Nitrobacter Glyphosate AAR22262 6.00E−54 Pseudomonas ABD1413 0.91 1.4.99.1 1245 414 0 417 53 772 oxidoreductase winogradskyi oxidoreductase aeruginosa [Nitrobacter Nb-255 gene polypeptide winogradskyi downstream #3. Nb-255] flanking region. 773, FAD dependent 92113847 1.00E−149 Chromohalo- Glyphosate AAR22262 3.00E−42 Nocardiopsis ADR47152 0.23 1.4.99.1 1242 413 0 414 62 774 oxidoreductase bacter oxidoreductase alba [Chromohalo- salexigens gene copper stable bacter salexigens DSM 3043 downstream protease 08. DSM 3043] flanking region. 775, Glycine/D-amino 88810939 5.00E−77 Nitrococcus N. ABP30542 7.00E−64 Aspergillus ABT17832 3.7 1.4.99.1 1272 423 0 423 38 776 acid oxidase mobilis gonorrhoeae fumigatus [Nitrococcus Nb-231 nucleotide essential mobilis Nb-231] sequence SEQ gene protein gi|88791478|gb|E ID 4691. #821. AR22589.1] Glycine/D-amino acid oxidase [Nitrococcus mobilis Nb-231] 777, putative 27379412 0 Bradyrhizo- Glyphosate AAR22262 3.00E−42 Prokaryotic ACA26366 0.9 1.4.99.1 1233 410 0 410 92 778 oxidoreductase bium oxidoreductase essential gene protein japonicum gene #34740. [Bradyrhizobium USDA 110 downstream japonicum flanking USDA 110] region. 779, FAD dependent 118037424 0 Burkholderia Glyphosate AAR22262 5.00E−49 Rice abiotic ACL30152 0.23 1.4.99.1 1233 410 0 465 86 780 oxidoreductase phytofirmans oxidoreductase stress [Burkholderia PsJN gene responsive phytofirmans downstream polypeptide PsJN] flanking SEQ ID gi|117992233|gb| region. NO: 4152. EAV06525.1| FAD dependent oxidoreductase [Burkholderia phytofirmans PsJN] 781, FAD dependent 118593299 1.00E−122 Stappia Glyphosate AAR22262 2.00E−47 Stress tolerant AEA26962 0.06 1.4.99.1 1272 423 0 422 52 782 oxidoreductase aggregata oxidoreductase plant-related [Stappia IAM 12614 gene transcription aggregata IAM downstream factor protein 12614] flanking SeqID10. gi|118434190|gb| region. EAV40846.1] FAD dependent oxidoreductase [Stappia aggregata IAM 12614] 783, FAD linked 149124512 7.00E−99 Methylo- FAD- ADM97925 9.00E−89 Bacterial ADT42301 9.00E−10 1.1.2.4 1137 378 0 477 51 784 oxidase domain bacterium dependent-D- polypeptide protein sp. 4-46 erythronate 4- #10001. [Methylobacte- phosphate rium sp. 4-46] dehydrogenase. 785, FAD dependent 114319576 1.00E−84 Alkalilim- Pseudomonas ABO75104 3.00E−76 Pseudomonas ABD08815 0.001 1.4.99.1 1251 416 0 421 41 786 oxidoreductase nicola aeruginosa aeruginosa [Alkalilimnicola ehrlichei polypeptide polypeptide ehrlichei MLHE-1 #3. #3. MLHE-1] 787, FAD dependent 144897812 9.00E−85 Magneto- Pseudomonas ABO75104 2.00E−81 Human breast AAL13181 0.001 1.4.99.1 1251 416 0 420 42 788 oxidoreductase spirillum aeruginosa cancer [Magnetospirillum gryphiswal- polypeptide expressed gryphiswaldense dense #3. polynucleotide MSR-1] MSR-1 8440. 789, putative 126732124 1.00E−107 Sagittula Glyphosate AAR22262 1.00E−40 Prokaryotic ACA39870 0.23 1.4.99.1 1248 415 0 410 46 790 oxidoreductase stellata E-37 oxidoreductase essential gene protein [Sagittula gene #34740. stellata E-37] downstream gi|126707413|gb| flanking EBA06477.1| region. putative oxidoreductase protein [Sagittula stellata E-37] 791, Oxidoreductase, 89211549 2.00E−98 Halo- Propioni- ABM37055 9.00E−80 Human soft ADQ24247 3 1.3.1.26 1044 347 0 333 50 792 N-terminal: thermothrix bacterium tissue Dihydrodi orenii acnes predicted sarcoma- picolinate H 168 ORF-encoded upregulated reductase polypeptide protein - [Halothermothrix #300. SEQ ID 40. orenii H 168] gi|89158855|gb|E AR78543.1| Oxidoreductase, N-terminal: Dihydrodi picolinate reductase [Halothermothrix orenii H 168] 793, PUTATIVE 15964495 1.00E−149 Sinorhizo- Glyphosate AAR22262 8.00E−46 Plant ADT18820 0.004 1. . . 1233 410 1254 417 63 65 794 OXIDO- bium oxidoreductase polypeptide, REDUCTASE meliloti gene SEQ ID PROTEIN downstream 5546. [Sinorhizobium flanking meliloti]. region. 795, indolepyruvate 118593297 0 Stappia Acinetobacter ADA35170 0 Silicibacter sp. AEM45684 0.007 1.2.7.8 2313 770 0 1173 61 796 ferredoxin aggregata baumannii TM1040 gene oxidoreductase IAM 12614 protein SEQ ID [Stappia #19. NO: 3. aggregata IAM 12614] gi|118434188|gb| EAV40844.1| indolepyruvate ferredoxin oxidoreductase [Stappia aggregata IAM 12614] 797, FAD linked 149124512 1.00E−128 Methylo- FAD- ADM97925 1.00E−113 Bacterial ADT47055 1.00E−06 1.1.2.4 1437 478 0 477 53 798 oxidase domain bacterium dependent-D- polypeptide protein sp. 4-46 erythronate 4- #10001. [Methylobacte- phosphate rium sp. 4-46] dehydrogenase. 799, FAD dependent 114319576 9.00E−90 Alkalilim- M. ADL05210 4.00E−63 Human ACH13524 0.015 1.4.99.1 1281 426 0 421 42 800 oxidoreductase nicola catarrhalis endothelial [Alkalilimnicola ehrlichei protein #1. cell cDNA ehrlichei MLHE-1 #2412. MLHE-1] 801, FAD dependent 144897812 1.00E−89 Magneto- Pseudomonas ABO75104 8.00E−76 Bacterial ADS63429 0.23 1.4.99.1 1257 418 0 420 44 802 oxidoreductase spirillum aeruginosa polypeptide [Magneto- gryphiswal- polypeptide #10001. spirillum dense #3. gryphiswaldense MSR-1 MSR-1] 803, FAD dependent 114319576 1.00E−86 Alkalilim- P. ADQ03060 4.00E−78 Plant ADJ41976 0.92 1.4.99.1 1257 418 0 421 42 804 oxidoreductase nicola aeruginosa cDNA [Alkalilimnicola ehrlichei virulence #31. ehrlichei MLHE-1 gene VIR14, MLHE-1] protein. 805, FAD linked 149124512 1.00E−121 Methylo- FAD- ADM97925 1.00E−104 N. ABZ40619 4.00E−09 1.1.2.4 1323 440 0 477 53 806 oxidase domain bacterium dependent-D- gonorrhoeae protein sp. 4-46 erythronate 4- nucleotide [Methylobacte- phosphate sequence rium sp. 4-46] dehydrogenase. SEQ ID 4691. 807, D-amino acid 149377918 1.00E−135 Marinobacter Photorhabdus ABM69115 5.00E−84 Novel human AAF66669 0.25 1.4.99.1 1329 442 0 423 55 808 dehydrogenase algicola luminescens polynucleotide, small subunit DG893 protein SEQ ID [Marinobacter sequence #59. NO: 1975. algicola DG893] 809, FAD dependent 118038076 0 Burkholderia Glyphosate AAR22262 6.00E−46 Bifido- ABQ81849 0.91 1.4.99.1 1242 413 0 413 81 810 oxidoreductase phytofirmans oxidoreductase bacterium [Burkholderia PsJN gene longum phytofirmans downstream NCC2705 PsJN] flanking ORF gi|117991720|gb| region. amino acid EAV06013.1| sequence FAD dependent SEQ ID oxidoreductase NO: 408. [Burkholderia phytofirmans PsJN] 811, FAD dependent 118038076 0 Burkholderia Glyphosate AAR22262 6.00E−46 Bifido- ABQ81849 0.91 1.4.99.1 1242 413 0 413 81 812 oxidoreductase phytofirmans oxidoreductase bacterium [Burkholderia PsJN gene longum phytofirmans downstream NCC2705 PsJN] flanking ORF gi|117991720|gb| region. amino acid EAV06013.1| sequence FAD dependent SEQ ID oxidoreductase NO: 408. [Burkholderia phytofirmans PsJN] 813, FAD dependent 118038076 0 Burkholderia Glyphosate AAR22262 6.00E−46 Bifido- ABQ81849 0.91 1.4.99.1 1242 413 0 413 81 814 oxidoreductase phytofirmans oxidoreductase bacterium [Burkholderia PsJN gene longum phytofirmans downstream NCC2705 PsJN] flanking ORF gi|117991720|gb| region. amino acid EAV06013.1| sequence FAD dependent SEQ ID oxidoreductase NO: 408. [Burkholderia phytofirmans PsJN] 815, FAD dependent 118038076 0 Burkholderia Glyphosate AAR22262 6.00E−46 Bifido- ABQ81849 0.91 1.4.99.1 1242 413 0 413 81 816 oxidoreductase phytofirmans oxidoreductase bacterium [Burkholderia PsJN gene longum phytofirmans downstream NCC2705 PsJN] flanking ORF gi|117991720|gb| region. amino acid EAV06013.1| sequence FAD dependent SEQ ID oxidoreductase NO: 408. [Burkholderia phytofirmans PsJN] 817, FAD dependent 114319576 5.00E−93 Alkalilim- P. ADQ03060 1.00E−79 Enterobacter AEH53102 0.004 1.4.99.1 1269 422 0 421 43 818 oxidoreductase nicola aeruginosa cloacae [Alkalilimnicola ehrlichei virulence protein ehrlichei MLHE-1 gene VIR14, amino acid MLHE-1] protein. sequence - SEQ ID 5666. 819, FAD dependent 144897812 1.00E−124 Magneto- P. ADQ03060 2.00E−99 Human ABN17150 0.015 1.4.99.1 1263 420 0 420 54 820 oxidoreductase spirillum aeruginosa ORFX [Magneto- gryphiswal- virulence protein spirillum dense gene VIR14, sequence gryphiswaldense MSR-1 protein. SEQ ID MSR-1] NO: 19716. 821, FAD dependent 92113847 1.00E−150 Chromohalo- Glyphosate AAR20642 2.00E−43 Pseudomonas ABD10634 0.058 1.4.99.1 1242 413 0 414 62 822 oxidoreductase bacter oxidoreductase aeruginosa [Chromohalo- salexigens gene polypeptide bacter salexigens DSM 3043 downstream #3. DSM 3043] flanking region. 823, hypothetical 13473721 1.00E−176 Mesorhizo- Human AAE00797 2.00E−76 Human AAV72125 2.00E−04 1.5.3.1 1170 389 1167 388 77 824 protein, contains bium Her-2/neu catalyc weak similarity to loti over telomerase sarcosine oxidase expression sub-unit [Mesorhizobium modulated PCR primer loti]. protein HTR2BAM. (HOMPS) H14 cDNA. 825, FAD dependent 118731339 0 Delftia Pseudomonas ABO71517 1.00E−100 Pseudomonas ABD04986 1.00E−12 1.1.99. 1146 381 0 385 90 826 oxidoreductase acidovorans aeruginosa aeruginosa [Delftia SPH-1 polypeptide polypeptide acidovorans #3. #3. SPH-1] gi|118668198|gb| EAV74793.1| FAD dependent oxidoreductase [Delftia acidovorans SPH-1] 827, FAD dependent 118593299 1.00E−122 Stappia Glyphosate AAR22262 2.00E−47 Stress tolerant AEA26962 0.06 1.4.99.1 1272 423 0 422 52 828 oxidoreductase aggregata oxidoreductase plant-related [Stappia IAM gene transcription aggregata IAM 12614 downstream factor protein 12614] flanking SeqID10. gi|118434190|gb| region. EAV40846.1| FAD dependent oxidoreductase [Stappia aggregata IAM 12614] 829, FAD dependent 114319576 2.00E−87 Alkalilim- Pseudomonas ABO75104 1.00E−66 Pseudomonas ABD08815 6.00E−05 1.4.99.1 1281 426 0 421 40 830 oxidoreductase nicola aeruginosa aeruginosa [Alkalilimnicola ehrlichei polypeptide polypeptide ehrlichei MLHE-1 #3. #3. MLHE-1] 831, hypothetical 13475565 7.00E−99 Mesorhizo- Pseudomonas ABO71517 1.00E−78 Pseudomonas ADB04986 2.00E−11 1.1.99. 1137 378 1152 383 49 59 832 protein bium aeruginosa aeruginosa [Mesorhizobium loti polypeptide polypeptide loti]. #3. #3. 833, FAD dependent 144897812 2.00E−89 Magneto- Pseudomonas ABO75104 8.00E−76 Bacterial ADS63429 0.23 1.4.99.1 1257 418 0 420 44 834 oxidoreductase spirillum aeruginosa polypeptide [Magneto- gryphiswal- polypeptide #10001. spirillum dense #3. gryphiswaldense MSR-1 MSR-1] 835, FAD linked 149124512 1.00E−123 Methylo- FAD- ADM97925 1.00E−110 Bacterial ADS56646 8.00E−14 1.1.2.4 1395 464 0 477 52 836 oxidase domain bacterium dependent-D- polypeptide protein sp. 4-46 erythronate 4- #10001. [Methylobacte- phosphate rium sp. 4-46] dehydrogenase. 837, D-aspartate 115376852 1.00E−56 Stigmatella Human AED18771 8.00E−45 Acinetobacter AEK59509 0.68 1.4.3.1 942 313 0 314 39 838 oxidase aurantiaca D-aspartate species rpoB [Stigmatella DW4/3-1 oxidase gene SEQ aurantiaca active site. ID NO: 19. DW4/3-1] gi|115366155|gb| EAU65167.1| D-aspartate oxidase [Stigmatella aurantiaca DW4/3-1] 839, FAD dependent 118038076 0 Burkholderia Glyphosate AAR22262 6.00E−46 Bifido- ABQ81849 0.91 1.4.99.1 1242 413 0 413 81 840 oxidoreductase phytofirmans oxidoreductase bacterium [Burkholderia PsJN gene longum phytofirmans downstream NCC2705 PsJN] flanking ORF gi|117991720|gb| region. amino acid EAV06013.1| sequence FAD dependent SEQ ID oxidoreductase NO: 408. [Burkholderia phytofirmans PsJN] 841, FAD dependent 71909453 1.00E−126 Dechloro- P. ADQ03060 9.00E−93 Human ABN17150 2.00E−05 1.4.99.1 1269 422 0 418 52 842 oxidoreductase monas aeruginosa ORFX [Dechloromonas aromatica virulence protein aromatica RCB] RCB gene VIR14, sequence protein. SEQ ID NO:. 19716 843, FAD dependent 118029195 1.00E−180 Burkholderia Enterobacter AEH60497 1.00E−171 Pseudomonas ABD08815 1.00E−43 1.4.99.1 1290 429 0 428 72 844 oxidoreductase phymatum cloacae aeruginosa [Burkholderia STM815 protein polypeptide phymatum amino acid #3. STM815] sequence - gi|117985258|gb| SEQ ID 5666. EAU99635.1| FAD dependent oxidoreductase [Burkholderia phymatum STM815] 845, putative 27379412 0 Bradyrhizo- Glyphosate AAR22262 1.00E−42 Peptide #3 AAD56788 0.23 1.4.99.1 1233 410 0 410 93 846 oxidoreductase bium oxidoreduc- used for protein japonicum tase gene purifying [Bradyrhizobium USDA 110 downstream peptidyl-tRNA japonicum flanking hydrolase USDA 110] region. (PTH) protein. 847, FAD dependent 77457196 1.00E−171 Pseudomonas Pseudomonas ADQ03060 7.00E−80 Pseudomonas ABD05088 2.00E−51 1.1.99. 1131 376 0 375 77 848 oxidoreductase fluorescens aeruginosa aeruginosa [Pseudomonas PfO-1 polypeptide polypeptide fluorescens #3. #3. PfO-1] 849, FAD dependent 114319576 7.00E−93 Alkalilim- P. Enterobacter AEH53102 0.004 1.4.99.1 1269 422 0 421 43 850 oxidoreductase nicola aeruginosa cloacae [Alkalilimnicola ehrlichei virulence protein ehrlichei MLHE-1 gene VIR14, amino acid MLHE-1] protein. sequence - SEQ ID 5666. 851, D-amino acid 32140775 1.00E−62 Arthrobacter Primer ADF68144 4.00E−63 Myco- AAI99682 0.18 1.4.3.3 975 324 4E+06 326 852 oxidase protophor- Aprev4 bacterium [Arthrobacter miae #SEQ ID 8. tuberculosis protophormiae]. strain H37Rv genome SEQ ID NO 2. 853, FAD dependent 77457196 1.00E−171 Pseudomonas Pseudomonas ABO71517 1.00E−151 Pseudomonas ABD05088 2.00E−51 1.1.99. 1131 376 0 375 77 854 oxidoreductase fluorescens aeruginosa aeruginosa [Pseudomonas PfO-1 polypeptide polypeptide fluorescens #3. #3. PfO-1] 855, FAD dependent 118051673 0 Comamonas Pseudomonas ABO71517 1.00E−99 Pseudomonas ABD04986 1.00E−05 1.1.99. 1173 390 0 390 99 856 oxidoreductase testosteroni aeruginosa aeruginosa [Comamonas KF-1 polypeptide polypeptide testosteroni #3. #3. KF-1] gi|118001016|gb| EAV15172.1| FAD dependent oxidoreductase [Comamonas testosteroni KF-1] 857, FAD dependent 118051673 0 Comamonas Pseudomonas ABO71517 1.00E−99 Pseudomonas ABD04986 1.00E−05 1.1.99. 1173 390 0 390 99 858 oxidoreductase testosteroni aeruginosa aeruginosa [Comamonas KF-1 polypeptide polypeptide testosteroni #3. #3. KF-1] gi|118001016|gb| EAV15172.1| FAD dependent oxidoreductase [Comamonas testosteroni KF-1] 859, FAD dependent 118051673 0 Comamonas Pseudomonas ABO71517 1.00E−99 Pseudomonas ABD04986 1.00E−05 1.1.99. 1173 390 0 390 99 860 oxidoreductase testosteroni aeruginosa aeruginosa [Comamonas KF-1 polypeptide polypeptide testosteroni #3. #3. KF-1] gi|118001016|gb| EAV15172.1| FAD dependent oxidoreductase [Comamonas testosteroni KF-1] 861, D-amino acid 108803375 3.00E−70 Rubrobacter Human AED18771 8.00E−62 Yeast AED11687 5.00E−08 1.4.3.3 987 328 0 326 46 862 oxidase xylanophilus D-aspartate D-amino [Rubrobacter DSM 9941 oxidase acid oxidase. xylanophilus active site. DSM 9941] 863, D-amino acid 66043505 0 Pseudomonas P. ADQ03060 0 Pseudomonas ABD08815 0 1.4.99.1 1299 432 0 433 88 864 dehydrogenase syringae pv. aeruginosa aeruginosa small subunit syringae virulence polypeptide [Pseudomonas B728a gene VIR14, #3. syringae pv. protein. syringae B728a] 867, 868 Methano- ADS43070 3.00E−37 0.003 2.6.1.9 1062 353 12392 373 30 96 coccus maripaludis 869, C7 AEM18037 1.00E−126 2.00E−25 2.6.1.21 852 283 1140 283 78 80 870 871, Planctomyces ADN26446 1.00E−95 1.1 5.4.3.8 1350 449 3784 417 46 96 872 maris DSM 8797 873, Planctomyces ADN26446 7.00E−94 0.071 5.4.3.8 1401 466 1358 417 44 96 874 maris DSM 8797 875, Roseiflexus ADN26446 8.00E−98 1.00E−09 5.4.3.8 1344 447 37500 417 48 88 876 castenholzii DSM 13941 877, Planctomyces ADN26446 2.00E−94 0.018 5.4.3.8 1389 462 231 417 43 100  878 maris DSM 8797 879, Planctomyces ADN26446 2.00E−93 0.018 5.4.3.8 1398 465 1356 417 43 96 880 maris DSM 8797 881, Oceanobacter AEB37927 3.00E−50 2.7 2.6.1.21 873 290 1653 282 39 100  882 sp. RED65 883, Thiobacillus AEM18040 2.00E−49 2.6.1.21 292 283 42 884 denitrificans ATCC 25259 885, Clostridium EEM18031 2.00E−47 2.7 2.6.1.21 861 286 19976 283 40 100  886 beijerinckii NCIMB 8052 887, Nocardioides AEK20408 3.00E−27 0.63 2.6.1.42 801 266 799 284 37 96 888 sp. JS614 889, Methylococcus ABM71198 2.00E−53 0.66 2.6.1.21 840 279 264 282 41 100  890 capsulatus str. Bath 891, Clostridium ABB08244 2.00E−47 0.67 2.6.1.21 861 286 599 283 39 100  892 beijerinckii NCIMB 8052 893, Clostridium ABB08244 5.00E−46 0.17 2.6.1.21 861 286 44577 283 37 100  894 beijerinckii NCIMB 8052 895, Clostridium ABU32980 1.00E−45 0.66 2.6.1.21 849 282 16714 289 39 100  896 aceto- butylicum ATCC 824 897, Roseiflexus ADN26446 5.00E−93 7.00E−08 5.4.3.8 1353 450 1287 417 47 82 898 castenholzii DSM 13941 899, Planctomyces ADN26446 1.00E−100 1.00E−09 5.4.3.8 1362 453 37500 417 47 91 900 maris DSM 8797 901, Planctomyces ADN26446 1.00E−104 0.001 5.4.3.8 1386 461 1341 417 49 96 902 maris DSM 8797 903, Planctomyces ADN26446 7.00E−99 0.07 5.4.3.8 1383 460 870 417 47 84 904 maris DSM 8797 905, Rhodobacter ADF03944 3.00E−68 2.7 2.6.1.21 864 287 5766 298 46 100  906 sphaeroides 2.4.1 907, Streptomyces AEK20408 2.00E−33 0.042 2.6.1.42 831 276 591 284 36 100  908 avermitilis MA-4680 909, Bacillus sp. ADW43694 1.00E−159 0 2.6.1.21 855 284 1709 284 97 88 910 B14905 911, Azoarcus sp. ABU33175 4.00E−45 0.17 2.6.1.21 876 291 4862 278 36 100  912 EbN1 913, Bacillus AEM18039 2.00E−56 2.6 2.6.1.21 852 283 7166 282 43 100  914 licheniformis ATCC 14580 915, Roseiflexus ADN26446 2.00E−87 2.00E−05 5.4.3.8 1383 460 1701 417 41 81 916 castenholzii DSM 13941 917, Robiginitalea AAY13560 3.00E−48 0.72 2.6.1.21 915 304 652 282 39 100  918 biformata HTCC2501 919, Planctomyces ADN26446 1.00E−93 1.1 5.4.3.8 1350 449 18471 417 46 96 920 maris DSM 8797 921, Planctomyces ADN26446 6.00E−96 0.07 5.4.3.8 1377 458 20250 417 48 100  922 maris DSM 8797 923, Roseobacter ADF03944 5.00E−75 2.7 2.6.1.21 861 286 864 298 50 100  924 sp. MED193 925, Planctomyces ADN26446 1.00E−90 0.004 5.4.3.8 1377 458 1251 417 44 96 926 maris DSM 8797 927, Aquifex ADN17496 0 0 2.6.1.1 1185 394 1185 394 100 100  928 aeolicus VF5 929, Aspergillus ADS78245 0 2.6.1.42 322 317 100 930 terreus NIH2624 931, Oceanicola ADS78325 0 2.6.1.62 519 461 100 932 granulosus HTCC2516 933, Pyrococcus ADS41897 5.00E−53 3.8 2.6.1.1 1206 401 9502 387 33 100  934 horikoshii OT3 935, Aeromonas ADS78291 0 0 2.6.1.62 1383 460 1383 460 100 100  936 hydrophila subsp. hydrophila ATCC7966 937, Silibacter sp. ADF03944 9.00E−69 2.6 2.6.1.21 855 284 2000 298 48 95 938 TM1040 939, Rhodo- ADF03944 1.00E−53 0.67 2.6.1.21 855 284 86941 298 39 100  940 pseudomonas palutris CGA009 941, Xanthobacter ADF03944 8.00E−55 2.7 2.6.1.21 879 292 2840 298 41 100  942 autotrophicus Py2 943, Azoarcus sp. AEM18040 8.00E−53 0.17 2.6.1.21 870 289 1899 283 40 100  944 BH72 945, Clostridium AEM18031 2.00E−46 0.011 2.6.1.21 855 284 636 283 39 100  946 beijerinckii NCIMB 8052 947, Alcnivorax AAY13560 2.00E−54 0.042 2.6.1.21 837 278 897 282 40 91 948 borkumensis SK2 951, aspartate 14590640 2.00E−52 Pyrococcus Bacterial ADS41897 5.00E−53 Tumour AAS46730 3.6 2.6.1.1 1206 401 9502 387 952 aminotransferase horikoshii polypeptide suppressor [Pyrococcus #10001. gene derived horikoshii]. chemiclly modified sequence #530. 953, aspartate 15606968 0 Aquifex Bacterial ADN17496 0 Bacterial ADS45406 0 2.6.1.1 1185 394 0 394 100 954 aminotransferase aeolicus polypeptide polypeptide [Aquifex aeolicus]. #10001. #10001. 955, conserved 115385557 1.00E−126 Aspergillus Amino- ADS78245 1.00E−170 Amino- ADS78244 0 2.6.1.42 879 292 954 317 956 hypothetical terreus transferase/ transferase/ protein NIH2624 mutase/ mutase/ [Aspergillus deaminase deaminase terreus enzyme #14. enzyme #14. NIH2624] 957, hypothetical 89070918 0 Oceanicola Amino- ADS78325 0 Amino- ADS78324 0 2.6.1.62 1366 461 0 460 72 958 protein granulosus transferase/ transferase/ OG2516J 5919 HTCC2516 mutase/ mutase/ [Oceanicola deaminase deaminase granulosus enzyme #14. enzyme #14. HTCC2516] gi|89043511|gb|E AR49723.1| hypothetical protein OG2516J5919 [Oceanicola granulosus HTCC2516] 959, aminotrans- 117619456 1.00E−158 Aeromonas Amino- ADS78291 0 Amino- ADS78290 0 2.6.1.62 1383 460 1383 460 960 ferase; class III hydrophila transferase/ transferase/ [Aeromonas subsp. mutase/ mutase/ hydrophila hydrophila deaminase deaminase subsp. ATCC 7966 enzyme #14. enzyme #14. hydrophila ATCC 7966] 

What is claimed is:
 1. A method of converting tryptophan to indole-3-pyruvate or indole-3-pyruvate to tryptophan, comprising combining tryptophan or indole-3-pyruvate, respectively, with a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:219, wherein said nucleic acid molecule encodes the polypeptide comprising SEQ ID NO:220; or b) a variant of a), wherein said variant comprises a variant nucleic acid molecule that encodes a variant polypeptide, wherein said variant polypeptide has at least 95% sequence identity to SEQ ID NO:220 and has aminotransferase activity.
 2. The method of claim 1, wherein said variant encodes a variant polypeptide that has at least 99% sequence identity to SEQ ID NO:220 and has aminotransferase activity.
 3. The method of claim 1, wherein said variant polypeptide is a mutant and the mutant is selected from one or more of the mutations shown in Table 43 or Table
 52. 4. The method of claim 1, wherein the variant nucleic acid molecule has been codon optimized.
 5. The method of claim 1, wherein the nucleic acid molecule or variant nucleic acid molecule is contained within an expression vector.
 6. The method of claim 5, wherein the nucleic acid molecule or variant nucleic acid molecule is overexpressed in an isolated transformed host cell.
 7. The method of claim 1, wherein the polypeptide or variant polypeptide is immobilized on a solid support.
 8. The method of claim 1, wherein the variant polypeptide is a chimeric polypeptide.
 9. A method of converting tryptophan to indole-3-pyruvate or indole-3-pyruvate to tryptophan, comprising combining tryptophan or indole-3-pyruvate, respectively, with a polypeptide comprising the amino acid sequence of SEQ ID NO:220.
 10. The method of claim 9, wherein the polypeptide is immobilized on a solid support.
 11. A method of converting tryptophan to indole-3-pyruvate or indole-3-pyruvate to tryptophan, comprising combining tryptophan or indole-3-pyruvate, respectively, with a variant polypeptide that has mat least 95% sequence identity to SEQ ID NO:220 and (ii) aminotransferase activity.
 12. The method of claim 11, wherein the variant polypeptide is a mutant selected from one or more mutations shown in Table
 52. 13. The method of claim 11, wherein said variant polypeptide has (i) at least 99% sequence identity to SEQ ID NO:220 and (ii) aminotransferase activity.
 14. The method of claim 13, wherein the variant polypeptide is a mutant selected from one or more mutations shown in Table
 43. 15. The method of claim 11, wherein the variant polypeptide is immobilized on a solid support.
 16. The method of claim 11, wherein the variant polypeptide is a chimeric polypeptide. 